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Muelleria pseudogibbula, a new species from a newly recorded genus(Bacillariophyceae) in China

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Journal of Oceanology and Limnology 2018年第2期36卷 556-558页

作者： LIU Qi LI Bo wang quanxi School of Life Science Shanxi University Taiyuan 030006 China College of Life and Environment Science Shanghai Normal University Shanghai 200234 China

The genus Muelleria is reported for the first time from China in this paper. The new diatom species Muelleria pseudogibbula sp. nov. was observed during a survey of diatoms from Zoig6 Wetland, Sichuan Province, China. The morphological characters of this new species were documented by light and scanning electron microscope and compared with similar species. M. pseudogibbula is characterized by its lanceolate to linear-lanceolate valve outline with rounded apices, two longitudinal canals running parallel to each side of the raphe, proximal ends of the raphe deflected to the same directions and finishing before the first row of areolae, and curved distal raphe ends, forming two divergent branches.

关键词： Muelleria new species new record genus Qinghai-Tibet wetland

Lineaperpetua ***.:a new diatom genus in the Thalassiosirales supported by morphology and molecular data

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Journal of Oceanology and Limnology 2024年第1期42卷 277-290页

作者： Pan YU Lin YANG Qingmin YOU John Patrick KOCIOLEK Kangyu wang Yonghong BI quanxi wang College of Life Sciences Shanghai Normal UniversityShanghai 200234China Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China Museum of Natural History and Department of Ecology and Evolutionary Biology University of ColoradoUCB 218Boulder CO 80309USA

Based on a combination of morphology and molecular data of ribosomal DNA genes,a new diatom genus Lineaperpetua ***,You,Kociolek&wang is *** features that help define Lineaperpetua at the level of genus include:a tangentially undulated valve face;continuous cribra areolae on the valve interior consisting of pores arranged as strips;single rimoportula located inside the ring of marginal ***,phylogenetic analysis based on nuclear small subunit(SSU)rDNA sequences and nuclear large subunit(LSU)rDNA gene placed the three strains of *** in a single,monophyletic clade at a considerable sequence distance from the other genera(Thalassiosira,Conticribra,Planktoniella,Shinodiscus,and other genera)belonging to *** the similarities with some species of Thalassiosira,Conticribra,and Spicaticribra,the suite of features found in Lineaperpetua differentiate it from these other *** molecular data and morphological characters suggest an affinity of the new genus to the Thalassiosiraceae.

关键词： diatom morphology new genus phylogeny taxonomy Thalassiosiraceae

Deletion of an electron donor-binding subunit of the NDH-1 complex, NdhS, results in a heat-sensitive growth phenotype in Synechocystis sp. PCC 6803

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Chinese Science Bulletin 2014年第33期59卷 4484-4490页

作者： Jiaohong Zhao Fudan Gao Zijian Qiu quanxi wang Weimin Ma College of Life Sciences and Technology Harbin Normal University College of Life and Environment Sciences Shanghai Normal University

In cyanobacteria and higher plants, NdhS is suggested to be an electron donor-binding subunit of NADPH dehydrogenase(NDH-1) complexes and its absence impairs NDH-1-dependent cyclic electron transport around photosystem I(NDH-CET). Despite significant advances in the study of NdhS during recent years, its functional role in resisting heat stress is poorly ***, our results revealed that the absence of NdhS resulted in a serious heat-sensitive growth phenotype in the unicellular cyanobacterium Synechocystis sp. strain PCC6803. Furthermore, the rapid and significant increase in NDH-CET caused by heat treatment was completely abolished, and the repair of photosystem II under heat stress conditions was greatly impaired when compared to that of other photosynthetic apparatus in the thylakoid membrane. We therefore conclude that NdhS plays an important role in resistance to heat stress, possibly by stabilizing the electron input module of cyanobacterial NDH-1 complexes.

关键词：电子给体复合物集胞藻表型生长热敏亚单位光系统II

Two new Stephanodiscaceae species(Bacillariophyta)from SW China

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Journal of Oceanology and Limnology 2024年第6期42卷 2024-2033页

作者： Pan YU Fen LUO Qingmin YOU John Patrick KOCIOLEK Wanting PANG Yue CAO quanxi wang School of Environmental and Geographical Sciences Shanghai Normal UniversityShanghai 200235China College of Life Sciences Shanghai Normal UniversityShanghai 200234China College of Chemistry Biology and Environment Yuxi Normal UniversityYuxi 653100China Museum of Natural History and Department of Ecology and Evolutionary Biology University of ColoradoUCB 218Boulder CO 80309USA

Two new freshwater centric diatom species were described from the southwest of China based on light and scanning electron *** weixiense *** a transversely undulate valve face,marginal fultoportulae situated at the end of the hyaline strips,central fultoportulae forming a ring positioned in a circular pattern,each with 3-4(usually 3)satellite pores,and a single rimoportula located at the base of the inner distal end of the *** aspera *** an uneven valve face with small granules that may be present on the marginal area and valve mantle,marginal fultoportulae situated on each costa close to the margin of the valve,6-7 central fultoportulae forming a ring near the valve center each with two satellite pores,and one rimoportula located on the valve face/mantle junction in the middle of costa on the internal *** *** the first reported extant species in the *** study played an important role in studying the taxonomic status and ecological information of the genus,and the establishment of the two new taxa will help demonstrate the unique nature of freshwater diatom flora in the southwest of China.

关键词： diatom centric new species Pliocaenicus Tertiarius China

Morphological comparison and molecular marker screening of three Skeletonema species found in Changjiang(Yangtze)River Basin

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Journal of Oceanology and Limnology 2024年第3期42卷 923-942页

作者： Jingwen HU Yuxin YI Zhengxin YANG John Patrick KOCIOLEK Pan YU quanxi wang Qingmin YOU College of Life Sciences Shanghai Normal UniversityShanghai 200235China Museum of Natural History University of ColoradoBoulder 80309USA College of Environmental and Geographical Sciences Shanghai Normal UniversityShanghai 200235China

In our recent investigations of diatom diversity,we studied three species,namely,Skeletonema costatum,Skeletonema subsalsum,and Skeletonema *** they have been found frequently in Changjiang(Yangtze)River Basin,their morphological and molecular identification is difficult in ***,to integrate morphological and molecular biological approaches,we compared systematically their morphological characters and performed phylogenetic *** strains of Skeletonema were collected and isolated from Shanghai and Jiangsu,China,and their morphological characteristics were examined by light microscopy(LM)and the scanning electron microscopy(SEM).Based on morphological comparison,we determined that *** is easy to distinguish from the other two *** heavily silicified areolae,undulated or cleft distal ends of terminal fultoportula processes(TFPPs),absence of basal pores of fultoportula processes(FPPs),the rootlike protrusions of FPPs,and no interlocking connection are the stable characteristics that can be used to identify ***,there are only two features that can distinguish *** from ***,namely the location of terminal rimoportulae(TRPs)and the distal shape of *** addition,we amplified and sequenced nine common genetic markers from the strains,from which 101 sequences were obtained,constructed phylogenetic trees based on the nine genes and evaluated that seven genes can be used to identify ***,and revealed that *** is the closest known relative of ***,and only ATP synthetase beta-subunit gene(atp B)is able to distinguish them from each other,which strongly support that it is an effective molecular marker for *** work provided a theoretical basis for the taxonomic study of Skeletonema.

关键词： Skeletonema Changjiang River morphological change distinguishing morphological characteristics molecular marker

Qinia ***.(Bacillariophyceae:Cymbellales)from Yunnan Province,China

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Journal of Oceanology and Limnology 2023年第5期41卷 1965-1977页

作者： Yan LIU John Patrick KOCIOLEK Maxim KULIKOVSKIY Anton GLUSHCHENKO Pan YU quanxi wang Xinxin LU Yawen FAN College of Life Science and Technology Harbin Normal UniversityHarbin 150025China Museum of Natural History and Department of Ecology and Evolutionary Biology University of ColoradoBoulderCO 80309USA K.А.Timiryazev Institute of Plant Physiology RAS IPP RASMoscow 127276Russia College of Life Sciences Shanghai Normal UniversityShanghai 200234China

An interesting group of cymbelloid diatoms was collected from lakes in Yunnan Province,China,and observations on their valve morphology made using light and scanning electron *** cymbelloid species all have apical pore fields,slit-like areolae,and areolar occlusions,and they all lack of *** this paper we propose the new genus Qinia *** these taxa and describe them as new:*** ***.,*** ***.,and *** *** genus Qinia *** lanceolate valves asymmetrical about their apical axes,raphe located in the middle of valve,distal raphe ends bent to the dorsal margin that bisects the apical pore fields(APFs),no stigmata,areolae with slit-like external openings and projections occluding the areolar openings *** genus resembles the genus Cymbella in the presence of APFs,but differs from Cymbella by a lack of stigmata,and by having external distal raphe ends that bisect the apical pore *** Reimeria,the external distal raphe ends bisect the lobes of the APF,but Qinia species differ from Reimeria in that they have no stigmata and the distal raphe ends are deflected dorsally,not *** were made between Qinia and other cymbelloid genera whose members possess apical pore *** that distinguish each species of the new genus are also discussed.

关键词： freshwater new genus cymbelloid diatom freshwater morphology

Detection and Characterization of a Novel Norovirus in Bats,China

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Virologica Sinica 2018年第1期33卷 100-103页

作者： Ling'en Yang quanxi wang Lin Xu Changchun Tu Xiaohong Huang Biao He College of Animal Science Fujian A & F UniversityFuzhou 350002 China Key Laboratory of Jilin Province for Zoonosis Prevention and Control Military Veterinary Institute Academy of Military Medical Sciences Changchun 130122 China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis Yangzhou 225009 China

Dear Editor,Noroviruses(No Vs)are second only to the rotaviruses as etiologic agents of acute fulminant gastroenteritis in infants and young children worldwide,with an estimated 200,000deaths per year in children younger than 5 years of age in developing countries(Patel et al.2008).No Vs are classified within the genus Norovirus of the family Caliciviridae with Norwalk virus as its prototype member(ICTV 2017).The virions are small(38–40 nm in diameter)nonenveloped,with an icosahedral capsidanda linear,positive-

关键词：蝙蝠中国世界范围肠胃炎暴发性代理人孩子病毒

A case study on Landscape Component Niche based on Landscape Pattern Indices: Yanchi,Ningxia Province, China

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Research in Cold and Arid Regions 2020年第1期12卷 22-33页

作者： XiaoNing Zhang XueLu Liu Ghanney Philip XiaoDan Li quanxi wang YaNan Xu TingTing Hui College of Resources and Environmental Sciences Gansu Agricultural UniversityLanzhouGansu 730070China College of Management Gansu Agricultural UniversityLanzhouGansu 730070China

Yanchi County is located in the agro-pastoral ecotone and belongs to the ecologically fragile area of Northwest *** is important to study the evolution of landscape pattern to curb its environmental *** order to intuitively show how the landscape pattern of the study area changes over time,Landsat Thematic Mappers(TM)and Landsat Operational Land Imager(OLI)data of 1991,2000,2010 and 2017 were *** paper attempts to apply niche theories and methods into landscape ecology,and constructs a niche model of landscape components by using"n-dimentional hypervolume niche theory"and landscape pattern *** evaluating the spatial and temporal evolution of niche from the perspective of two-dimensional space to reflect the changes of landscape pattern in the study area over the past 26 years,new theories and methods were introduced for the characterization of landscape *** results indicate that:1)The larger the attribute and dominance value of landscape components,the higher the ecological niche and the stronger the control effect on the overall landscape.2)The ecological niche of each landscape component was significantly different,just as its control effect on the overall landscape.3)The dynamic change of the ecological niche of each landscape component was different,with grassland,unused land and arable land always in a high dominant position,although the ecological niche of construction land and water area was always *** general,the introduction of niche theory into the landscape ecology provided a new method to study the changes in regional landscape pattern.

关键词： landscape pattern landscape component niche landscape stability landscape dominance

The response of electron transport mediated by active NADPH dehydrogenase complexes to heat stress in the cyanobacterium Synechocystis 6803

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Science China(Life Sciences) 2008年第12期51卷 1082-1087页

作者： MA WeiMin, WEI LanZhen & wang quanxi College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China 1. College of Life and Environment Sciences Shanghai Normal University Shanghai 200234 China

The electron-transport machinery in photosynthetic membranes is known to be very sensitive to heat. In this study, the rate of electron transport (ETR) driven by photosystem I (PSI) and photosystem II (PSII) during heat stress in the wild-type Synechocystis sp. strain PCC 6803 (WT) and its ndh gene inactiva-tion mutants △ndhB (M55) and △ndhD1/ndhD2 (D1/D2) was simultaneously assessed by using the novel Dual-PAM-100 measuring system. The rate of electron transport driven by the photosystems (ETRPSs) in the WT, M55, and D1/D2 cells incubated at 30℃ and at 55℃ for 10 min was compared. Incubation at 55 ℃ for 10 min significantly inhibited PSII-driven ETR (ETRPSII) in the WT, M55 and D1/D2 cells, and the ex-tent of inhibition in both the M55 and D1/D2 cells was greater than that in the WT cells. Further, PSI-driven ETR (ETRPSI) was stimulated in both the WT and D1/D2 cells, and this rate was increased to a greater extent in the D1/D2 than in the WT cells. However, ETRPSI was considerably inhibited in the M55 cells. Analysis of the effect of heat stress on ETRPSs with regard to the alterations in the 2 active NDH-1 complexes in the WT, M55, and D1/D2 cells indicated that the active NDH-1 supercomplex and medi-umcomplex are essential for alleviating the heat-induced inhibition of ETRPSII and for accelerating the heat-induced stimulation of ETRPSI, respectively. Further, it is believed that these effects are most likely brought about by the electron transport mediated by each of these 2 active NDH-1 complexes.

关键词： active NDH-1 complexes heat response photosystems-driven electron transport Synechocystis 6803