Deletion of an electron donor-binding subunit of the NDH-1 complex, NdhS, results in a heat-sensitive growth phenotype in Synechocystis sp. PCC 6803

作     者：Jiaohong Zhao Fudan Gao Zijian Qiu Quanxi Wang Weimin Ma 

作者机构：College of Life Sciences and Technology Harbin Normal University College of Life and Environment Sciences Shanghai Normal University 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2014年第59卷第33期

页      面：4484-4490页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the National Natural Science Foundation of China(31370270) National Basic Research Program of China(2009CB118500) Shanghai Natural Science Foundation(14ZR1430000) Project of Shanghai Education Committee(12ZZ132) 

主　　题：电子给体 复合物 集胞藻 表型 生长 热敏 亚单位 光系统II 

摘      要：In cyanobacteria and higher plants, NdhS is suggested to be an electron donor-binding subunit of NADPH dehydrogenase(NDH-1) complexes and its absence impairs NDH-1-dependent cyclic electron transport around photosystem I(NDH-CET). Despite significant advances in the study of NdhS during recent years, its functional role in resisting heat stress is poorly ***, our results revealed that the absence of NdhS resulted in a serious heat-sensitive growth phenotype in the unicellular cyanobacterium Synechocystis sp. strain PCC6803. Furthermore, the rapid and significant increase in NDH-CET caused by heat treatment was completely abolished, and the repair of photosystem II under heat stress conditions was greatly impaired when compared to that of other photosynthetic apparatus in the thylakoid membrane. We therefore conclude that NdhS plays an important role in resistance to heat stress, possibly by stabilizing the electron input module of cyanobacterial NDH-1 complexes.