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Adrenomedullin inhibits the endothelin production induced by urotensin Ⅱ in rat vascular smooth muscle cells

Chinese Science Bulletin 2002年第17期47卷 1457-1461页

作者： ql yongfen BU Dingfang YANG Jun ZHANG Zhaokang SHI Yanrong PANG Yongzheng TANG Chaoshu Institute of Cardiovascular Diseases The First HospitalPeking UniversityBeijing 100034China Phoenix Pharmaceutical Inc.2438 Wyandotte Street Montain View Department of Physiology Healh Science CenterPeking UniversityBeijing 100083China

The aim of this study was to observe the effects of adrenomedullin (ADM) on endothelin (ET) production induced by urotensin Ⅱ (UⅡ) in rat vascular smooth muscle cells (VSMCs). Cultured VSMCs which were incubated with UⅡ (10-8 mol/L) and with various concentrations of ADM were used to measure the VSMCs 3H-TdR incorporation, the activity of extracellular signal-regulated kinase (ERK), the amount of ET mRNA and ET production in VSMCs. In this work we found that incubation with UⅡ (10-8 mol/L) increased obviously the amount of ET mRNA in VSMCs and ET production in medium, however, co-incubation with ADM (10-10-10-8 mol/L) and UⅡ(10-8 mol/L) reduced the amount of ET mRNA by 15%, 24% and 45% (P< 0.01) respectively, compared with UⅡ alone. The content of ET in medium was 14.13, 11.38 and 11.00 pg/mL. ADM alone (10-8 mol/L) had no effect on ET production in VSMCs. UⅡ (10-8 mol/L) promoted the 3H-TdR incorporation and activity of ERK in VSMCs. ADM inhibited VSMCs 3H-TdR incorporation and activation of

关键词： adrenomedullin urotensin II endothelin mRNA pep-tide/interaction.

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Urotensin Ⅱ increases endothelin production by vascular smooth muscle cells in rats

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Chinese Science Bulletin 2002年第12期47卷 1007-1010页

作者： ql yongfen, BU Dingfang, YANG Jun, ZHANG Zhaokang, NIU Dadi, SHI Yanrong, ZHANG Zhenghao, TANG Chaoshu & DU JunbaoInstitute of Cardiovascular Diseases, The First Hospital of Peking University, Beijing 100083, China Department of Physiology, Health Science Institute of Cardiovascular Diseases First Hospital of Peking University Beijing 100083 China

The aim of this study was to observe the effects of urotensin Ⅱ (UII) on production of endothelin (ET) in rat aortic vascular smooth muscle cells (VSMC). Cultured VSMCs incubated with various concentrations of UII were used to measure the YSMC 3H-TdR incorporation, the amount of ET mRNA and ET production in VSMCs. In this work we found that UII (10-10-10-8 mol/L) promoted VSMC 3H-TdR incorporation (47%-83%, P<0.01) and increased the amount of ET mRNA by 17.1% (P < 0.05) to 112.8% (P < 0.01), respectively, in a concentration dependent manner compared with control. After 4 and 8 h incubation, 10-10-10-8 mol/L of UII elevated the ET synthesis and release in a concentration dependent manner. After 4 h incubation, the content of ET in medium was 4.9, 5.36 and 7.12 pg/mL (P < 0.01). After 8 h incubation, the ET content released from VSMCs was 12.6, 12.07 and 17.17 pg/mL (P < 0.01). In addition, it was found that BQ123, a specific ETA receptor antagonist, obviously decreased the VSMC DNA synthesis induced by

关键词： urotensin II endothelin mRNA peptide/interaction.

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