Urotensin Ⅱ increases endothelin production by vascular smooth muscle cells in rats

作     者：Ql Yongfen, BU Dingfang, YANG Jun, ZHANG Zhaokang, NIU Dadi, SHI Yanrong, ZHANG Zhenghao, TANG Chaoshu & DU JunbaoInstitute of Cardiovascular Diseases, The First Hospital of Peking University, Beijing 100083, China Department of Physiology, Health Science 

作者机构：Institute of Cardiovascular Diseases First Hospital of Peking University Beijing 100083 China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2002年第47卷第12期

页      面：1007-1010页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：This work was supported by the state major basic research development program (Grant No. G2000056905) 

主　　题：urotensin II endothelin mRNA peptide/interaction. 

摘      要：The aim of this study was to observe the effects of urotensin Ⅱ (UII) on production of endothelin (ET) in rat aortic vascular smooth muscle cells (VSMC). Cultured VSMCs incubated with various concentrations of UII were used to measure the YSMC 3H-TdR incorporation, the amount of ET mRNA and ET production in VSMCs. In this work we found that UII (10-10-10-8 mol/L) promoted VSMC 3H-TdR incorporation (47%-83%, P0.01) and increased the amount of ET mRNA by 17.1% (P 0.05) to 112.8% (P 0.01), respectively, in a concentration dependent manner compared with control. After 4 and 8 h incubation, 10-10-10-8 mol/L of UII elevated the ET synthesis and release in a concentration dependent manner. After 4 h incubation, the content of ET in medium was 4.9, 5.36 and 7.12 pg/mL (P 0.01). After 8 h incubation, the ET content released from VSMCs was 12.6, 12.07 and 17.17 pg/mL (P 0.01). In addition, it was found that BQ123, a specific ETA receptor antagonist, obviously decreased the VSMC DNA synthesis induced by