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财税政策对数字产业不同政策目标下的激励差异分析

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商业经济 2024年第4期 45-49,157页

作者：侯艺轩李齐云山东大学经济学院山东济南250100

我国数字产业在发展上面临着创新不足和效益低下的问题,需要采用税收优惠和政府补助政策来更好地引导数字产业的发展,但由于信息不对称的存在,导致两种政策的激励效果存在差异。针对两种政策对企业研发投入和企业利润的影响进行比较分析... 详细信息

我国数字产业在发展上面临着创新不足和效益低下的问题,需要采用税收优惠和政府补助政策来更好地引导数字产业的发展,但由于信息不对称的存在,导致两种政策的激励效果存在差异。针对两种政策对企业研发投入和企业利润的影响进行比较分析,研究发现:税收优惠、政府补助的激励效果存在差异;税收优惠、政府补助对不同数字产业的激励效果存在差异;在同一产业中,政府的不同政策目标也会影响到税收优惠和政府补助的政策效果。具体而言,当政府以提高企业研发投入为目标时,政府补助的效果要强于税收优惠。当政府以增加企业净利润为政策目标时,税收优惠的政策效果要强于政府补助。同时从宏观层面和微观层面提供相关建议。

关键词：税收优惠政府补助研发投入净利润

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斜纹夜蛾高致病力生防真菌的筛选与鉴定

中国生物防治学报 2024年第2期40卷 282-290页

作者：田艺帆张正坤隋丽赵宇路杨孟钊石旺鹏李启云吉林省农业科学院植物保护研究所/农业农村部东北作物有害生物综合治理重点实验室/吉林省农业微生物重点实验室长春130033 中国农业大学植物保护学院北京100083 公主岭市气象局公主岭136100

本研究从斜纹夜蛾幼虫僵虫中分离并纯化虫生真菌,得到6株昆虫病原真菌,利用形态学和分子生物学方法对上述真菌进行鉴定,采用浸渍法测定6株真菌对斜纹夜蛾2龄幼虫的毒力。结果表明,6株虫生真菌分别为2株球孢白僵菌Beauveria bassiana,以... 详细信息

本研究从斜纹夜蛾幼虫僵虫中分离并纯化虫生真菌,得到6株昆虫病原真菌,利用形态学和分子生物学方法对上述真菌进行鉴定,采用浸渍法测定6株真菌对斜纹夜蛾2龄幼虫的毒力。结果表明,6株虫生真菌分别为2株球孢白僵菌Beauveria bassiana,以及莱氏绿僵菌Metarhizium rileyi,木贼镰刀菌Fusarium equiseti,曲霉菌Aspergillus nomiae和糙孢蓝状菌Talaromyces trachyspermus各1株。在1×10^(8)个孢子/m L浓度下,LT50分别为4.737 d,4.543 d,1.343 d,2.086 d,2.212 d和2.351 d,6株虫生真菌对斜纹夜蛾2龄幼虫均表现出较强致病力,其中莱氏绿僵菌表现最好。本研究首次报道了镰刀菌和曲霉菌能够感染斜纹夜蛾,并首次发现糙孢蓝状菌能够感染昆虫。

关键词：斜纹夜蛾虫生真菌毒力

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真菌病毒Beauveria bassiana chrysovirus 2(BbCV2)外壳蛋白多克隆抗体制备及应用

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中国生物防治学报 2024年第3期40卷 583-591页

作者：路杨郭文博隋丽张庆贺赵宇张正坤李启云吉林省农业科学院植物保护研究所/吉林省农业微生物重点实验室/农业农村部东北作物有害生物综合治理重点实验室长春136100 吉林农业大学植物保护学院长春130118 吉林省农业环境保护与农村能源管理总站长春130021 吉林农业科技学院吉林132101

球孢白僵菌Beauveriabassiana是一种在害虫生物防治中应用广泛的虫生真菌,真菌病毒能够影响球孢白僵菌的致病力和生物学性状,但真菌病毒相关蛋白的抗体制备和利用其进行病毒检测和定位鲜有报道。本研究原核表达了一株普遍流行并且造成... 详细信息

球孢白僵菌Beauveriabassiana是一种在害虫生物防治中应用广泛的虫生真菌,真菌病毒能够影响球孢白僵菌的致病力和生物学性状,但真菌病毒相关蛋白的抗体制备和利用其进行病毒检测和定位鲜有报道。本研究原核表达了一株普遍流行并且造成寄主球孢白僵菌毒力下降的双链RNA(double stranded RNA,dsRNA)病毒Beauveria bassiana chrysovirus 2(Bb CV2)的外壳蛋白(Coat protein,CP),并制备了多克隆抗体,进行了病毒在寄主球孢白僵菌胞内和胞外的检测,以及利用间接免疫荧光进行病毒在寄主内的定位。结果表明,表达的BbCV2-CP蛋白主要以包涵体的形式存在,相对分子质量约为85.7kD;经间接EliSA方法测定,制备的BbCV2-CP兔源多抗效价达到1:8192000;Westernblot结果显示,制备的BbCV2兔源多抗能与抗原蛋白进行特异性结合,且能检测出感染寄主球孢白僵菌的BbCV2;EliSA测定结果表明病毒BbCV2可在寄主真菌体内复制,并能够游离到寄主体外;经间接免疫荧光观察,发现BbCV2病毒定位于真菌细胞核中。本研究建立了球孢白僵菌真菌病毒的血清学检测体系,为球孢白僵菌-真菌病毒互作机制研究提供材料。

关键词：球孢白僵菌真菌病毒BbCV2 多克隆抗体免疫荧光亚细胞定位

球孢白僵菌病毒BbCV2的流行及其遗传多样性

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中国生物防治学报 2024年第3期40卷 592-599页

作者：刘洪宇牛昕泽孙旸赵宇路杨隋丽李启云张正坤吉林农业大学生命科学学院长春130118 吉林省农业科学院植物保护研究所/农业农村部东北作物有害生物综合治理重点实验室/吉林省农业微生物重点实验室长春136100

球孢白僵菌Beauveria bassian是一种重要的虫生真菌,广泛用于害虫生物防治,毒力退化一直是制约其应用的重要因素。真菌病毒能够影响寄主真菌毒力。为明确一株能够降低寄主毒力的产黄青霉科病毒Beauveria bassiana chrysovirus2(BbCV2)... 详细信息

球孢白僵菌Beauveria bassian是一种重要的虫生真菌,广泛用于害虫生物防治,毒力退化一直是制约其应用的重要因素。真菌病毒能够影响寄主真菌毒力。为明确一株能够降低寄主毒力的产黄青霉科病毒Beauveria bassiana chrysovirus2(BbCV2)真菌病毒在田间球孢白僵菌种群中流行性规律,本试验收集了来源于吉林省7个不同地点采集的寄主为亚洲玉米螟Ostrinia furnacalis的球孢白僵菌菌株,通过dsRNA提取和PCR验证BbCV2的感染情况,并进行遗传多样性分析。结果表明,不同采集地的球孢白僵菌均检测到dsRNA病毒感染,尤其是BbCV2在吉林省内分布广泛,菌株带毒率为70%~100%。对BbCV2依赖RNA的RNA聚合酶基因(RdRp)进行遗传多样性分析发现,该病毒不同分离物间RdRp的核苷酸序列和氨基酸序列高度保守,相似度高达99.78%和99.98%,该病毒遗传相对稳定,并且其分布和遗传进化与地理位置无关。由此可见,BbCV2的流行是自然发生的,可能是造成球孢白僵菌田间毒力退化的重要因素,并且球孢白僵菌对该病毒适应性较强,对病毒的遗传选择压力不明显。

关键词：球孢白僵菌真菌病毒遗传进化生物防治

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“洪氏一针法”在胰十二指肠切除术中的应用

沈阳医学院学报 2024年第2期26卷 131-135页

作者：胡继龙李其云张秉韬郑志难孙丽江西省肿瘤医院腹部肿瘤外科江西南昌330029 江西省万年县中医院外一科江西省肿瘤医院药剂科

目的:探讨“洪氏一针法”在胰十二指肠切除术(PD)术中应用效果。方法:按照随机数字表法将2021年1月至2022年12月于我院行PD术治疗的40例患者分为2组,各20例。对照组采用胰腺空肠端端套入吻合术,观察组采用“洪氏一针法”行胰肠吻合术。... 详细信息

目的:探讨“洪氏一针法”在胰十二指肠切除术(PD)术中应用效果。方法:按照随机数字表法将2021年1月至2022年12月于我院行PD术治疗的40例患者分为2组,各20例。对照组采用胰腺空肠端端套入吻合术,观察组采用“洪氏一针法”行胰肠吻合术。比较2组围手术期指标、并发症、二次手术及死亡率、生活质量。结果:观察组胰肠吻合时间、手术时间及住院时间均短于对照组,胰瘘发生率低于对照组,差异有统计学意义(P0.05);观察组术后精神健康评分、情感职能评分、社会功能评分、精力评分、一般健康状况评分、躯体疼痛评分、生理职能评分、生理机能评分高于对照组,差异有统计学意义(P<0.05)。结论:PD术中采用“洪氏一针法”行胰肠吻合术有利于缩短胰肠吻合时间、手术时间、住院时间,加快患者术后恢复,减少胰瘘发生率,提高患者生活质量。

关键词：胰十二指肠切除术 “洪氏一针法” 胰腺空肠端端套入吻合术并发症胰肠吻合时间

Lack of ethylene does not affect reproductive success and synergid cell death in Arabidopsis

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Molecular Plant 2022年第2期15卷 354-362页

作者： Wenhao li qiyun li Mohan Lyu Zhijuan Wang Zihan Song Shangwei Zhong Hongya Gu Juan Dong Thomas Dresselhaus Sheng Zhong li-Jia Qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences at College of Life SciencesPeking UniversityBeijing 100871People's Republic of China The National Plant Gene Research Center(Beijing) Beijing 100101People's Republic of China The Waksman Institute of Microbiology Rutgers the State University of New JerseyPiscatawayNJ 08854USA Cell Biology and Plant Biochemistry University of Regensburg93053 RegensburgGermany

The signaling pathway of the gaseous hormone ethylene is involved in plant reproduction,growth,devel-opment,and stress *** reproduction,the two synergid cells of the angiosperm female gametophyte both undergo programmed cell death(PCD)/degeneration but in a different manner:PCD/degeneration of one synergid facilitates pollen tube rupture and thereby the release of sperm cells,while PCD/degeneration of the other synergid blocks supernumerary pollen *** signaling was postu-lated to participate in some of the synergid cell functions,such as pollen tube attraction and the induction of PCD/***,ethylene-mediated induction of synergid PCD/degeneration and the role of ethylene itself have not been firmly ***,we employed the CRISPR/Cas9 technology to knock out the five ethylene-biosynthesis 1-aminocyclopropane-1-carboxylic acid oxidase(ACO)genes and created Arabidopsis mutants free of ethylene *** ethylene-free mutant plants showed normal triple responses when treated with ethylene rather than 1-aminocyclopropane-1-carboxylic acid,but had increased lateral root density and enlarged petal sizes,which are typical phenotypes of mutants defective in ethylene *** these ethylene-free plants,we further demonstrated that production of ethylene is not necessarily required to trigger PCD/degeneration of the two synergid cells,but certain com-ponents of ethylene signaling including transcription factors ETHYLENE-INSENSITIVE 3(EIN3)and EIN3-liKE 1(EIL1)are necessary for the death of the persistent synergid cell.

关键词： ethylene ACC PCD synergid cells triple response Arabidopsis

Engineered extracellular vesicles efficiently deliver CRISPR-Cas9 ribonucleoprotein(RNP)to inhibit herpes simplex virus1 infection in vitro and in vivo

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1362-1379页

作者： Yuanda Wan liren li Ruilin Chen Jiajia Han qiyun Lei Zhipeng Chen Xiaodong Tang Wenyu Wu Shuwen liu Xingang Yao NMPA Key Laboratory for Research and Evaluation of Drug Metabolism Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Center of Clinical Pharmacy Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Key Laboratory of Infectious Diseases Research in South China(Southern Medical University) Ministry of EducationGuangzhou 510515China

Extracellular vesicles(EVs)have recently emerged as a promising delivery platform for CRISPR/Cas9 ribonucleoproteins(RNPs),owing to their ability to minimize off-target effects and immune ***,enhancements are required to boost the efficiency and safety of Cas9 RNP enrichment within *** response,we employed the Fc/Spa interaction system,in which the human Fc domain was fused to the intracellular domain of PTGFRN-Δ687 and anchored to the EV ***,the B domain of the Spa protein was fused to the C domain of cargos such as Cre or *** to the robust interaction between Fc and Spa,this method enriched nearly twice the amount of cargo within the *** loaded with spCas9 RNP targeting the HSV1 genome exhibited significant inhibition of viral replication in vitro and in ***,following neuron-targeting peptide RVG modification,the in vivo dosage in neural tissues substantially increased,contributing to the clearance of the HSV1 virus in neural tissues and exhibiting a lower off-target *** findings establish a robust platform for efficient EV-based SpCas9 delivery,offering potential therapeutic advantages for HSV1 infections and other neurological disorders.

关键词： Extracellular vesicles CRISPR/Cas9 Ribonucleoproteins PTGFRN Fc/Spa HSV1 Neuron-targeting Delivery

Degradation of a fullerene end-capped polycaprolactone by lipase AK

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Chinese Science Bulletin 2010年第29期55卷 3279-3282页

作者： PENG qiyun KANG Feng li Juan YANG Xinlin School of life Science and Technology Beijing Institute of Technology Beijing 100081 China

Using titrimetric method,enzymatic behavior of lipase AK against fullerene end-capped polycaprolactone(C60-PCL) was studied for the first time,compared with polycaprolactone(PCL).The results showed that degradation of both C60-PCL and PCL by lipase followed Mechaelis-Menten equation,with the degradation parameters of PCL and C60-PCL were 0.75 mg mL-1 and 0.16 mg mL-1 for Km,and 0.90 mL h-1 and 0.43 mL h-1 for Vmax,respectively.C60-PCL had a slower degradation rate than PCL under the same *** data indicated that the introduction of fullerene enabled PCL hard to be degraded by lipase.

关键词：聚己内酯脂肪酶富勒烯降解酶封端 PCL C60 米氏方程

Spatio-temporal evolution and topographic gradient effect of land use and ecosystem service value in the Lhasa River Basin

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Journal of Mountain Science 2024年第6期21卷 2059-2074页

作者： ZHOU Jianwei FENG Bin WU Hua XU Tong CHEN linna ZHAO Xinyong GUO qiyun li Jiatong ZHANG Chenguang ZHU Kangcheng KONG Yuzhong School of Ecology and Environment Tibet UniversityLhasa 850000China School of Engineering Tibet UniversityLhasa 850000China Joint Laboratory of Plateau Surface Remote Sensing Tibet UniversityLhasa 850000China

The Lhasa River Basin forms an essential human settlement area in the southern part of the Qinghai-Tibet *** study employed ecosystem service value(ESV)evaluation model,terrain gradient grading,and Geodetector to analyze land use and ESV in the Lhasa River Basin from 1985 to *** findings reveal that:(1)From 1985 to 2020,grassland was the dominant land *** was a trend of grassland reduction and the expansion of other land types.(2)ESV has increased over the research period(with a total increase of 0.84%),with higher values in the southeast and lower values in the *** contributed the most to ESV,and climate regulation and hydrological regulation were the ecosystem services that contribute the most to ESV.(3)Natural factors like NDVI and altitude,as well as economic factors like population density and distance from roads,influenced the spatial differentiation of ESV,the explanatory power of NDVI reached up to *** interaction between factors had a greater impact than individual *** research results can provide theoretical support for national spatial planning and ecological environment protection in the Lhasa River Basin and other similar areas.

关键词： Ecosystem services value Land use Terrain gradient effect Lhasa River Basin Driving factors