Lack of ethylene does not affect reproductive success and synergid cell death in Arabidopsis
作者机构：State Key Laboratory for Protein and Plant Gene ResearchPeking-Tsinghua Center for Life Sciences at College of Life SciencesPeking UniversityBeijing 100871People's Republic of China The National Plant Gene Research Center(Beijing)Beijing 100101People's Republic of China The Waksman Institute of MicrobiologyRutgers the State University of New JerseyPiscatawayNJ 08854USA Cell Biology and Plant BiochemistryUniversity of Regensburg93053 RegensburgGermany
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2022年第15卷第2期
页 面：354-362页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：supported by the National Key R&D Program of China(grant no.2018YFE0204700)and National Natural Science Foundation of China(grant no.31991202 31830004 31620103903 and 31621001 toLJQ and 32070854 to Sheng Znong).The Qu laboratory is supported by the Peking-Tsinghua Joint Center for Life Sciences and work on frti-ization and eary embryogenesis in the Dresselhaus lab is supported by the German Research Foundation DFG via Collaborative Research Center SFB960
主 题：ethylene ACC PCD synergid cells triple response Arabidopsis
摘 要：The signaling pathway of the gaseous hormone ethylene is involved in plant reproduction,growth,devel-opment,and stress *** reproduction,the two synergid cells of the angiosperm female gametophyte both undergo programmed cell death(PCD)/degeneration but in a different manner:PCD/degeneration of one synergid facilitates pollen tube rupture and thereby the release of sperm cells,while PCD/degeneration of the other synergid blocks supernumerary pollen *** signaling was postu-lated to participate in some of the synergid cell functions,such as pollen tube attraction and the induction of PCD/***,ethylene-mediated induction of synergid PCD/degeneration and the role of ethylene itself have not been firmly ***,we employed the CRISPR/Cas9 technology to knock out the five ethylene-biosynthesis 1-aminocyclopropane-1-carboxylic acid oxidase(ACO)genes and created Arabidopsis mutants free of ethylene *** ethylene-free mutant plants showed normal triple responses when treated with ethylene rather than 1-aminocyclopropane-1-carboxylic acid,but had increased lateral root density and enlarged petal sizes,which are typical phenotypes of mutants defective in ethylene *** these ethylene-free plants,we further demonstrated that production of ethylene is not necessarily required to trigger PCD/degeneration of the two synergid cells,but certain com-ponents of ethylene signaling including transcription factors ETHYLENE-INSENSITIVE 3(EIN3)and EIN3-LIKE 1(EIL1)are necessary for the death of the persistent synergid cell.
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