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R2R3-type Lo MYB21 affects jasmonate-regulated development and dehiscence of anthers in lily(lilium oriental hybrids)

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Horticultural Plant Journal 2024年第1期10卷 239-251页

作者： Zheng Tong Tingting Dong qiuhua li Rui Wang Junna He Bo Hong Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences&Hainan Institute for Tropical Agricultural ResourcesHaikouHainan 571101China Beijing Key Laboratory of Development and Quality Control of Ornamental Crops Department of Ornamental HorticultureChina Agricultural UniversityBeijing 100193China Beijing Garden Expo Management Center Beijing 100072China

lilies are widely cultivated for cut flowers,but their large anthers carry a considerable amount of colored pollen that is dispersed easily.Studying the molecular mechanism of anther development and dehiscence could help solve this problem.LoMYB21,encoding a putative R2R3v-myb avian myeloblastosis viral oncogene homolog(MYB)transcription factor,was identified from oriental lilies(lilium‘Siberia’).Real-time quantitative PCR analysis showed that LoMYB21 was mainly expressed in the anther,filament and stigma and had high expression during the late stages of lily anther development.LoMYB21 had transactivation activity and was located in the nucleus through yeast one-hybrid assays and transient expression in Nicotiana benthamiana.Suppression of LoMYB21 by virus-induced gene silencing(VIGS)in lilium‘Siberia’led to anther indehiscence and reduced the expression of genes related to Jasmonate acid(JA)biosynthesis and signal transduction.Induction of LoMYB21 in DEX::LoMYB21 transgenic Arabidopsis caused procumbent inflorescences that became infertile,accompanied by higher expression of JA biosynthetic and signaling genes.These results demonstrated that JA content and signaling were abnormal in silenced lily and transgenic LoMYB21 Arabidopsis,which affected anther development.Our study indicated that LoMYB21 could regulate lily anther dehiscence through JA biosynthesis and signaling during the late stages of anther development.

关键词： lilium LoMYB21 Anther dehiscence Jasmonate acid

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Comparison in phytoplankton diversity-productivity-community stability between river-type reservoir and lake-type reservoir

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Journal of Oceanology and limnology 2022年第4期40卷 1485-1507页

作者： Qiong WU qiuhua li Huan LUO Qian CHEN Huaxiang CHEN Yanjun DONG Shenghua li College of Environment Hohai UniversityNanjing 210098China Pearl River Water Resources Research Institute Guangzhou 510611China Key Laboratory of the Pearl River Estuary Regulation and Protection of Ministry of Water Resources Guangzhou 510611China Guangdong Provincial Engineering Technology Research Center for life and Health of River&Lake Guangzhou 510611China Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province Guizhou Normal UniversityGuiyang 550001China Bureau of Ecological Environment of Xunwu County of Jiangxi Province Xunwu 342200China

Relationship between biodiversity and ecosystem function is one of the core issues in ecological research.Phytoplankton,as the main producer of aquatic ecosystem,its diversity,productivity,and community stability are of great signifi cance to reveal ecosystem function.There are signifi cant diff erences in hydrodynamics,water retention time,and phytoplankton community structure between river-type reservoir and newly built lake-type reservoir.The comparative analysis of phytoplankton community stability between the two types of reservoir has not been reported.Jiuquwan Reservoir(river-type)and Taihu Reservoir(lake-type),the two reservoirs in the Dongjiang River source area of Zhujiang(Pearl)River Basin,were selected for comparison in terms of multi-year operation vs.new impoundment,river-type vs.lake-type,and shallow water vs.sub-deep water reservoirs.Samples were collected in dry season(December 2019),normal season(March 2020),and wet season(August 2020),on which the phytoplankton diversity and productivity of the two reservoirs,and the relationship and diff erence of community stability were examined.Results show that(1)the number of phytoplankton species in Jiuquwan Reservoir decreased comparing that before algal bloom and the restoration treatment,while that in Taihu Reservoir increased compared with that before the impoundment of the reservoir.There was no signifi cant diff erence in functional groups and species number between the two reservoirs(P>0.05);(2)the biological stability,diversity,productivity,and resource utilization effi ciency of newly built lake-type reservoir were higher than those of multi-year river-type reservoir.In addition,the utilization effi ciency of phytoplankton resources was the highest in wet season in both reservoirs.The increases in biodiversity,richness,and evenness promoted the stability of the community,while increases in productivity and resource utilization effi ciency weakened the stability of the community;(3)community stability was aff ected by both biotic and abiotic fact

关键词： community stability functional group stability phytoplankton diversity resource utilization effi ciency reservoir

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The key environmental factors driving the succession of phytoplankton functional groups in Hongfeng Reservoir, southwest China

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Journal of Oceanology and limnology 2022年第4期40卷 1472-1484页

作者： libin HAN qiuhua li Wensheng CHEN Xing WANG Shihui ZHOU Mengshu HAN Anton BRANCELJ Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province Guizhou Normal UniversityGuiyang 550001China Guizhou International Science&Technology Cooperation Base-International Joint Research Centre for Aquatic Ecology Guizhou Normal UniversityGuiyang 550001China Key Laboratory for Information and Computing Science of Guizhou Province Guizhou Normal UniversityGuiyang 550001China National Institute of Biology Vecna pot 111Ljubljana 1000Slovenia

Reservoirs are an important water source in many densely populated areas in southwest China.Phytoplankton play an essential role in maintaining the structure and function of reservoir ecosystems.Understanding the succession in phytoplankton communities and the factors driving it are essential for eff ective water quality management in drinking water reservoirs.In this study,water samples were collected monthly at the surface layers from March 2016 to December 2019 in Hongfeng Reservoir,southwest China.The relationship between functional group succession was analyzed based on nonmetric multidimensional scaling analysis(NMDS),redundancy analysis(RDA),succession rate,and other analysis methods.The results showed distinct shifts in the community structure of phytoplankton functional groups within study period.The Cyclotella sp.was dominant in 2016 and 2017,and Pseudanabaena limnetica was the dominant group in 2018 and 2019.It appears that the phytoplankton composition and biomass are closely related to the water temperature and nutrient status in this reservoir.The results clearly showed that the permanganate index(COD_(Mn))was the key factor of dramatic phytoplankton functional group succession,and the change in succession rates was closely caused by total nitrogen concentration(TN).Therefore,the succession pattern and key factors of Hongfeng Reservoir revealed in this study were important guidance for the management of drinking water reservoirs in southwest China.A reasonable limit on exogenous nutrient input should be a priority,especially in high water temperature period.

关键词： phytoplankton functional groups driving factors nonmetric multidimensional scaling succession rates Redundancy analysis

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Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro

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Chinese Herbal Medicines 2022年第3期14卷 449-458页

作者： Shuai Wen Ran An Dongli li Junxi Cao Zhigang li Wenji Zhang Ruohong Chen qiuhua li Xingfei Lai lingli Sun Shili Sun Tea Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation&UtilizationGuangzhou510640China School of Biotechnology and Health Sciences Wuyi UniversityJiangmen 529020China International Healthcare Innovation Institute(Jiangmen) Jiangmen 529040China

Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)were analyzed.The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored.Methods:National standard method and HPLC were used for active ingredient analysis.MTT,cell fow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apoptosis,and its underlying molecular mechanism.Results:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester catechins,non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM.The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM.Three T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells,especially YT-CM and GT-CM.Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax,Bcl-2 and Caspase-3/9.YT-CM and GT-CM had better ability to change the signal pathway than BT-CM.Conclusion:In short,T-CMs,which combined different degrees of fermentation tea with C.maxima,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and GT-CM,are healthy drinks that help to prevent and treat liver cancer.

关键词： apoptosis Citrus maxima cv.Tomentosa liver cancer signaling pathway tea

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Effective CRISPR/Cas9-based genome editing in large yellow croaker (Larimichthys crocea)

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Aquaculture and Fisheries 2023年第1期8卷 26-32页

作者： qiuhua li Guangming Shao Yangyang Ding libing Xu Jianchun Shao Jingqun Ao Xinhua Chen Key Laboratory of Marine Biotechnology of Fujian Province Institute of OceanologyFujian Agriculture and Forestry UniversityFuzhou350002China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China Key Laboratory of Marine Biogenetic Resources Third Institute of OceanographyMinistry of Natural ResourcesXiamen361005China College of Ocean and Earth Sciences Xiamen UniversityXiamen361102China

The large yellow croaker (Larimichthys crocea) is an economically important marine species with the highest annual production among the farmed marine fishes in China. However, the aquaculture industry of this species is suffering from severe problems that include weakened disease resistance, decreased growth rate, and reduced meat quality due to frequent inbreeding. Genome editing, which has a huge potential for solving those problems by introducing favorable genetic changes, is not yet available for the large yellow croaker. Here, we pioneered the techniques of embryo microinjection and genome editing using the CRISPR/Cas9 system in this species. Recombinant plasmids encoding green fluorescent protein (GFP) were introduced into the fertilized eggs of L. crocea by microinjection before the chorion had hardened. A high survival rate (40%) and GFP-positive larvae rate (81.8%) were achieved, indicating that the microinjection technique in L. crocea was successfully established. On this basis, Cas9 mRNA and sgRNA targeting the tyrosinase a gene in L. crocea (Lc-tyra) were co-injected into fertilized eggs of L. crocea. Mutant individuals with insertion and deletion mutations of Lc-tyra were detected. These results indicated that the CRISPR/Cas9-based genome editing technology established herein could efficiently introduce mutations at a specific site in the L. crocea genome. This method provides the potential for genetic improvement and functional genomic study in this species. This is the first report on effective CRISPR/Cas9-based genome editing in L. crocea.

关键词： CRISPR/Cas9 Genome editing Large yellow croaker(Larimichthys crocea) Tyrosinase a gene

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Roles of Adinandra nitida(Theaceae) and camellianin A in HCl/ethanol-induced acute gastric ulcer in mice

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Food Science and Human Wellness 2022年第4期11卷 1053-1063页

作者： Erdong Yuan Yingyi lian qiuhua li Zhaoxiang Lai lingli Sun Xingfei Lai Ruohong Chen Shuai Wen Junquan Zhu Wenji Zhang Shili Sun School of Food Science and Engineering South China University of TechnologyGuangzhou 510641China Tea Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation&UtilizationGuangzhou 510640China Guangdong Society of Plant Protection Guangdong 510640China

Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.

关键词： Adinandra nitida(Theaceae) Camellianin A Gastric ulcer Oxidative stress Anti-inflammation

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Effects of typical algae species（Aphanizomenon flosaquae and Microcystis aeruginosa） on photoreduction of Hg2+ in water body

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Journal of Environmental Sciences 2019年第11期31卷 9-16页

作者： Rongguo Sun Yafei Mo Xinbin Feng Leiming Zhang lin Jin qiuhua li State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang China School of Chemistry and Material Guizhou Normal University Guiyang China Air Quality Research Division Science and Technology Branch Environment and Climate Change Canada Toronto Ontario Canada

Photoreduction characteristics of divalent inorganic mercury (Hg2+) in the presence of specific algae species are still not well known.Laboratory experiments were conducted in the present study to identify the effects of different concentrations of living/dead algae species,including Aphanizomenon flosaquae (AF) and Microcystis aeruginosa (MA),on the photoreduction rate of Hg2+ under various light conditions.The experimental results showed that percentage reduction of Hg2+ was significantly influenced by radiation wavelengths,and dramatically decreased with the presence of algae.The highest percentage reduction of Hg2+ was induced by UV-A,followed by UV-B,visible light and dark for both living and dead AF,and the order was dark > UV-A > UV-B > visible light for both living and dead MA.There were two aspects,i.e.,energy and attenuation rate of light radiation and excrementitious generated from algae metabolisms,were involved in the processes of Hg2+ photoreduction with the presence of algae under different light conditions.The percentage reduction of Hg2+ decreased from 15% to 11% when living and dead AF concentrations increased by 10 times (from 106 to 105 cells/mL),and decreased from11% to ~9% in the case of living and dead MA increased.Algae can adsorb Hg2+ and decrease the concentration of free Hg2+,thus inhibiting Hg2+ photoreduction,especially under the conditions with high concentrations of algae.No significant differences were found in percentage reduction of Hg2+ between living and dead treatments of algae species.The results are of great importance for understanding the role of algae in Hg2+ photoreduction.

关键词： Aphanizomenon flosaquae Microcystis aeruginosa Photoreduction Divalent inorganic mercury (Hg2+)

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2008—2022年结直肠癌的中医研究热点文献计量学分析

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中国医药科学 2024年第1期14卷 117-120页

作者：李戬李秋华辽宁中医药大学辽宁沈阳110032 辽宁中医药大学附属第二医院介入科辽宁沈阳110034

目的分析2008—2022年国内外结直肠癌的中医研究热点,为该领域未来的研究进展提供参考依据。方法从Web of Science核心合集数据库以及中国知网(CNKI)数据库中检索2008年1月1日至2022年10月1日结直肠癌中医相关领域的文献,使用VOSviewer... 详细信息

目的分析2008—2022年国内外结直肠癌的中医研究热点,为该领域未来的研究进展提供参考依据。方法从Web of Science核心合集数据库以及中国知网(CNKI)数据库中检索2008年1月1日至2022年10月1日结直肠癌中医相关领域的文献,使用VOSviewer以及CiteSpace软件对于文献的共被引网络以及高频关键词进行分析。结果Web of Science核心合集数据库检索文献917篇,CNKI数据库检索文献1257篇,中国为发文量最高的国家,上海中医药大学的研究数量居于国内前列。结论2008—2022年有关中医药治疗结直肠癌的研究逐年增长,未来该领域的研究热点可能以肠道菌群、肿瘤微环境以及结肠炎所致结直肠癌的研究为主。

关键词：中医药结直肠癌文献计量学可视化分析

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数字人类学视域下的产品创新设计

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设计 2024年第5期37卷 70-72页

作者：李秋华唐林涛清华大学美术学院

应用大数据思维为产品创新设计探索新方法,旨在推动大数据与产品创新设计理论的发展。运用跨学科的方法,归纳了数字人类学视域下大数据对产品创新设计的启发与价值。提出了大数据从生活方式、全景图和远读3个方面驱动了产品创新设计。... 详细信息

应用大数据思维为产品创新设计探索新方法,旨在推动大数据与产品创新设计理论的发展。运用跨学科的方法,归纳了数字人类学视域下大数据对产品创新设计的启发与价值。提出了大数据从生活方式、全景图和远读3个方面驱动了产品创新设计。产品创新设计将从对物的设计转向对数字化生活方式的设计,大数据驱动产品创新设计的变革。

关键词：大数据创新设计人类学生活方式全景图远读

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Monovalent cation perm-selective membranes(MCPMs)"New developments and perspectives

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Chinese Journal of Chemical Engineering 2017年第11期25卷 1606-1615页

作者： liang Ge Bin Wu Dongbo Yu Abhishek N.Mondal linxiao Hou Noor U1 Afsar qiuhua li Tingting Xu Jibin Miao Tongwen Xu CAS Key Laboratory of Soft Matter Chemistry Collaborative Innovation Center of Chemistry for Energy MaterialsSchool of Chemistry and Materials ScienceUniversity of Science and Technology of ChinaHefei 230026China

As one of the most typical and promising membrane processes, electrodialysis(ED) technique plays a more and more significant role in industrial separation. Especially, the separation of monovalent cations and multivalent cations is currently a hot topic, which is not only desirable for many industries but also challenging for academic explorations. The main aim of the present contribution is to view the advances of a wide variety of monovalent cation perm-selective membranes(MCPMs) and their preparation technologies including(1) covalent crosslinking,(2) surface modification,(3) polymer blending,(4) electrospinning,(5) nanofiltration alike membrane,and(6) organic–inorganic hybrid. The relevant advantages and disadvantages with respect to some specific cases have been discussed and compared in detail. Furthermore, we elaborately discuss the opportunities and challenges of MCPMs, the fabricating strategies to take and the future perspectives.

关键词： Ion exchange membranes Monovalrnt cation perm-selective membranes Electrodialysis Ion separation Electro- nanofiltr ation

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