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Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera

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International Journal of Ophthalmology(English edition) 2015年第1期8卷 39-45页

作者： Qing Wang Mei-Lan Xue Gui-qiu zhao Mei-Guang Liu Yu-Na Ma Yan Ma Department of Ophthalmology the Affiliated Hospital of Qingdao University Department of Biochemistry and Molecular Biology Medical College Qingdao University

AIM: To identify the presence of various bone morphogenetic proteins(BMPs) and their receptors in normal sclera of human, rat and guinea pigs, and to determine whether their expression changed with form-deprivation myopia(FDM) in guinea pig ***: The expression of BMPs and BMP receptors were detected using reverse transcription polymerase chain reaction(RT-PCR) and immunofluorescence. Two-week-old guinea pigs were monocularly form-deprived with a translucent lens. After fourteen days induction of FDM, total RNA was isolated and subjected to RT-PCR to examine the changes of BMPs and BMP receptors in tissues from the posterior sclera. Western blotting analysis was used to investigate their changes in protein ***: Human sclera expressed m RNAs for BMP-2,-4,-5,-7,-RIA,-RIB and BMP-RII. Conversely, rat sclera only expressed m RNA for BMP-7 and BMP-RIB,while the expression of BMPs and BMP receptors in guinea pigs were similar to that of humans. Human sclera also expresses BMP-2,-4,-5,-7 in protein *** days after the induction of myopia, significant decreased expressions for BMP-2 and BMP-5 in the posterior sclera of FDM-affected eyes(P <0.05 vs internal control eyes).· CONCLUSION: Various BMPs were expressed in human and guinea pig sclera. In the posterior sclera,expressions of BMP-2 and BMP-5 significantly decreased in FDM eyes. This finding indicates that various BMPs as components of the scleral cytokines regulating tissue homeostasis and provide evidence that alterations in the expression of BMP-2 and BMP-5 are associated with sclera remodeling during myopia induction.

关键词： form-deprivationmyopia bonemorphogenetic protein human sclera guinea pig sclera

Expression of macrophage migration inhibitory factor in Aspergillus fumigatus keratitis

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International Journal of Ophthalmology(English edition) 2019年第5期12卷 711-716页

作者： Qiang Xu Li-Ting Hu Qian Wang Jing Lin Nan Jiang Cui Li Gui-qiu zhao Department of Ophthalmology the Affiliated Hospital of Qingdao University

AIM: To investigate the expression of macrophage migration inhibitory factor(MIF) and detect its role in the innate immune response of fungal keratitis(FK). METHODS: We collected the paraffin-embedded cornea tissues from 10 FK and 6 ocular trauma patients to explore the MIF expression by immunohistochemistry. Then we cultured telomease-immortalized human corneal epithelial cells(THCEs), stimulated by the hyphae suspension of Aspergillus fumigatus(A. fumigatus) to detect the change of MIF with or without the pretreatment of MIF inhibitor [4-Iodo-6-phenylpyrimidine(4-IPP)] by real-time polymerase chain reaction(PCR). The protein level of MIF was also tested by immunohistochemistry, and the level of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) mRNA were compared between normal, hyphae stimulated and 4-IPP pretreated groups by real-time PCR to study the influence of MIF on the expression of TNF-α and IL-6. Corneal severity of rats' FK models was documented by clinical scores, and real-time PCR. Western blot and immunohistochemistry were used to test the expression of MIF, TNF-α and IL-6 in rats' ***: In the corneas of FK patients, there was much stronger expression of MIF than that in the normal group showed by immunohistochemistry. In cultured THCEs stimulated by A. fumigatus, the expression of MIF became stronger in both immunohistochemistry and PCR at 16, 24, 32 and 48 h post infection(p.i.; P<0.01, P<0.01, P<0.01, P<0.05). After pretreated with 4-IPP, the expression of MIF reduced at 4, 8, 16 h p.i.(P<0.05, P<0.05, P<0.05) and the downstream TNF-α and IL-6 decreased obviously(P<0.05, P<0.01). In rats with A. fumigatus keratitis, the relative mRNA and protein level of MIF increased than thosein the normal group by PCR(at 1 d: P<0.01, 3 d: P<0.01, 5 d: P<0.01), Western blot and immunohistochemistry. After blocked MIF with 4-IPP, the clinical outcomes of rat keratitis showed markedly reduced inflammatory response(P<0.01), with TNF-α and IL-6 decre

关键词： macrophage migration inhibitory factor fungal keratitis innate immune A. fumigatus corneal epithelial cells rats

Rapamycin liposome gutta inhibiting fungal keratitis of rats

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International Journal of Ophthalmology(English edition) 2019年第4期12卷 536-541页

作者： Zhen-Hua Zhang Feng Teng Qing-Xiu Sun Shu-Zhen Wang Chao Liu Gui-qiu zhao Department of Ophthalmology Qingdao Central Hospital the Second Clinical Hospital of Qingdao University Department of Ophthalmology the Affiliated Hospital of Qingdao University

AIM: To study the therapeutic effect of rapamycin liposome eyedrops on fungal keratitis(FK) and its effect on the expression of monocyte chemotactic protein-1(MCP-1).METHODS: This study adopted the thin film dispersion method to prepare rapamycin liposomes eyedrops, as well as used the orthogonal design to analyze and study main influencing factors that affected the quality of liposomes. Totally 96 healthy Wistar rats were randomly divided into four groups: normal control group(A), FK blank control group(B), FK blank liposomes control group(C), and 30 FK rapamycin liposome treatment group(D). Groups B, C, and D were first prepared as FK animal models. The corneal response was recorded in details on day 1, 3, 5, 7, and 14 after modeling. Six rats were obtained and immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) were used to detect the expression of MCP-1 protein and mRNA, ***: The severity of corneal lesions in the rapamycin treatment group was reduced, and the clinical score of the slit lamp examination was lower than that of Groups B and C(P<0.01). The expression of MCP-1 in rapamycin treatment group was significantly inhibited, comparing to that of groups B and C(P<0.01).CONCLUSION: Liposome is a good drug carrier for rapamycin. Rapamycin has a good therapeutic effect on FK. It can reduce FK fungal burden and significantly inhibit the expression of MCP-1 protein and mRNA.

关键词： fungal keratitis rapamycin liposome monocyte chemotactic protein-1 rats

Production of interleukin-1β related to mammalian target of rapamycin/Toll-like receptor 4 signaling pathway during Aspergillus fumigatus infection of the mouse cornea

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International Journal of Ophthalmology(English edition) 2018年第5期11卷 712-718页

作者： Rui Xu Jing Lin Gui-qiu zhao Cui Li Cheng-Ye Che Qiang Xu Min Liu Department of Ophthalmology the Affiliated Hospital of Qingdao University

AIM：To elucidate the effect of rapamycin on regulating the production of interleukin（IL）-1β in Aspergillus fumigatus（***）-induced keratitis and to verify whether the expression of IL-1β in *** keratitis is associated with the mammalian target of rapamycin（mT OR）/Toll-like receptor 4（TLR4） signaling ***：Fungal keratitis mouse models of susceptible C57 BL/6 mice were established using *** mice were subsequently treated with *** protein levels of p-mT OR,TLR4,and IL-1β in normal and infected corneal tissue were measured by Western *** TLR4 and IL-1β m RNA levels were determined by real-time polymerase chain reaction（PCR）.RESULTS：In C57 BL/6 mice,rapamycin treatment decreased the clinical scores and production of the pro-inflammatory cytokine,IL-1β.The expression of TLR4,stimulated by ***,was reduced as well when the mT OR signaling pathway was suppressed by ***：Rapamycin is beneficial for the outcome of fungal keratitis and has an inhibitory effect expression of the inflammatory cytokine IL-1β.The inhibitory effect on IL-1β expression can be associated with the mT OR/TLR4 signaling pathway in *** infection in mice.

关键词： keratitis interleukin-1β mammalian target of rapamycin Toll-like receptor 4 mice

Effects of COX-2 inhibitor NS-398 on IL-10 expression in rat fungal keratitis

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International Journal of Ophthalmology(English edition) 2011年第2期4卷 165-169页

作者： Na Li Cheng-Ye Che Li-Ting Hu Jing Lin Qing Wang and Gui-qiu zhao Department of Ophthalmology the Affiliated Hospital of Qingdao University Qingdao 266003 Shandong Province China

AIM: To investigate the expression of interleukin-10 (IL-10) and the effect of NS-398 (COX-2 inhibitor) on the expression of IL-10 in fungal keratitis in rats, and analyze its effects on anti-fungus immunity. METHODS: Ninety Wister rats were randomly divided into 3 groups. Group A was blank control group (10 eyes). Group B was fungal keratitis group (40 eyes). Group C was fungal keratitis group treated with NS-398 (40 eyes). PAS staining, 100g/L potassium hydroxide (KOH) smear and fungal culture confirmed the successful establishment of fungal keratitis model. After the central epithelium was scraped, Fusarium solani colonies were applied and contact lens was put on the right cornea of group B and C, and plane contact lens was put on the left cornea of control eyes. Phosphate buffered saline (PBS) eyedrops were given for group B and NS-398 eyedrops for group C. The expression of IL-10 on corneas of group B and C on the 1(st) day, 3(rd) days, 75(th) days, and 14(th) days were detected by immunohistochemistry and semi- quantitative reverse transcription- polymerase chain reaction (RT-PCR). RESULTS: Histopathologic examination showed neutrophil infiltration and severe tissue necrosis in ulcer cornea. PAS staining confirmed the existence of hyphae and spores in the superficial layer of stroma. In the blank and control groups almost no expression of IL-10 was detected at any observing points. In group B the expression of IL-10 increased at first and decreased thereafter. Its expression also showed significant difference at any observing points (P < 0.01). Compared with group B, the expression of IL-10 in group C showed no difference on the 1(st)day, decrease on the 3(rd) day, but a significant increase on the 7(th) day and 14(th) day. CONCLUSION: IL-10 takes part in the occurrence and development of fungal keratitis. NS-398 can upgrade the expression of IL-10 in fungal keratitis in the later period of the ulcer. Meanwhile, pathologic observation showed a slightly corneal

关键词： fungal keratitis immune regulation IL-10 NS-398

A new pattern of the tectonic units and metallogenic belts in Africa continent in terms of lithosphere

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China Geology 2022年第3期5卷 528-542页

作者： Rui-zhao qiu Ting-dong Li Qing-hui Xiao Kai Sun Yong Liu Lei qiu Xiu-fa Chen Hong-jun zhao Liang-liang Wang Quan-long Zhu Xiao-dong Ren Li-ke zhao Development and Research Centcer of China Geological Survey Ministry of Natural ResourcesBeijing 100037China Institute of Geology Chinese Academy of Geological SciencesMinistry of Natural ResourcesBeijing 100037China China University of Geosciences(Beijing) Beijing 100083China Tianjin Center China Geological SurveyTianjin300170China

Based on the comprehensive study of geology and geophysics in African continent,three types of lithosphere(craton-type,orogenic-type and rift-type)can be *** lithosphere discontinuities as the boundary,two first-order tectonic units(mainly cratonic-type in the west and rift-type in the east)are *** types of lithosphere can be divided into secondary-order and third-order structural units,and the blocks within lithosphere can be further divided into fourth-order structural *** geological history,the formation process and significance of different types of lithosphere in African continent are briefly discussed.

关键词： Lithospheric type Lithospheric discontinuity Tectonic unit division Africa continent

Evaluation of a new method for placing nasojejunal feeding tubes

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World Journal of Gastroenterology 2012年第37期18卷 5295-5299页

作者： Hua Qin Xiao-Yun Lu qiu zhao De-Min Li Pei-Yuan Li Mei Liu Qi Zhou Liang Zhu Hui-Fang Pang Hui-Zhen zhao Department of Gastroenterology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina

AIM:To compare fluoroscopic, endoscopic and guide wire assistance with ultraslim gastroscopy for placement of nasojejunal feeding tubes. METHODS:The information regarding nasojejunal tube placement procedures was retrieved using the gastrointestinal tract database at Tongji Hospital affiliated to Tongji Medical College. Records from 81 patients who underwent nasojejunal tubes placement by different techniques between 2004 and 2011 were reviewed for procedure success and tube-related outcomes. RESULTS:Nasojejunal feeding tubes were successfully placed in 78 (96.3%) of 81 patients. The success rate by fluoroscopy was 92% (23 of 25), by endoscopic technique 96.3% (26 of 27), and by guide wire assistance (whether via transnasal or transoral insertion)100% (23/23, 6/6). The average time for successful placement was 14.9 ± 2.9 min for fluoroscopic placement, 14.8 ± 4.9 min for endoscopic placement, 11.1 ± 2.2 min for guide wire assistance with transnasal gastroscopic placement, and 14.7 ± 1.2 min for transoral gastroscopic placement. Statistically, the duration for the third method was significantly different (P < 0.05) compared with the other three methods. Transnasal placement over a guidewire was significantly faster (P < 0.05) than any of the other approaches. CONCLUSION:Guide wire assistance with transnasal insertion of nasojejunal feeding tubes represents a safe, quick and effective method for providing enteral nutrition.

关键词： Enteral nutrition Nasojejunal feeding tube Guide wire assistance Fluoroscopy Endoscopy

Role of the IL-33/ST2/p38 signaling pathway in the immune response of corneal epithelial cells to Aspergillus fumigatus infection

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International Journal of Ophthalmology(English edition) 2019年第4期12卷 549-556页

作者： Jia You Jing Lin Yi-Fan Zhou Xu-Dong Peng Hong He Cui Li Guo-Qiang Zhu Xue-Qi zhao Gui-qiu zhao Department of Ophthalmology the Affiliated Hospital of Qingdao University Department of Clinical Laboratory the Affiliated Hospital of Qingdao University

AIM: To investigate the expression of interleukin(IL)-33 in the cornea and human corneal epithelial cells(HCECs) exposed to Aspergillus fumigatus(A. fumigatus), and to determine the function of IL-33/ST2/p38 signaling pathway in the immune response of corneal epithelial cells to A. fumigatus ***: The mRNA and protein expression of IL-33 in HCECs and mice corneas were examined by quantitative real-time reverse transcription polymerase chain reaction(q RT-PCR) and Western blot analysis, respectively. IL-33 expression was also detected in cornea samples from healthy donors and patients with fungal keratitis with immunohistochemistry. The cultured HCECs were treated with inactive A. fumigatus hyphae at various concentrations with or without recombinant human IL-33 protein, soluble recombinant ST2 protein, specific ST2 neutralizing antibody, or the mitogen-activated protein kinase(MAPK) p38 inhibitor SB203580 for evaluation of the expression and activation of IL-33/ST2/p38 signaling in the regulation of proinflammatory cytokines. The production levels of IL-6 and IL-1β were determined by qR T-PCR and enzymelinked immunosorbent assay(ELISA). The proliferation of HCECs was determined by a Cell Counting Kit-8(CCK8) assay and cell ***: IL-33 expression levels increased in the corneal tissues of patients with fungal keratitis and in mice corneas of experimental A. fumigatus infection,as well as in HCECs with infection of A. fumigatus. A. fumigatus strongly stimulated HCECs-generated proinflammatory cytokine(IL-6 and IL-1β) production at both the mRNA and protein levels. This production of proinflammatory mediators stimulated by A. fumigatus was further stimulated by IL-33 and was prevented by soluble ST2 protein or ST2 neutralizing antibody. Moreover, IL-33 naturally promoted the p38 phosphorylation induced by A. fumigatus, which was suppressed by soluble ST2 protein. The MAPK p38 inhibitor SB203580 also inhibited the A. fumigatus-induced proinflammato

关键词： ST2 interleukin 33 Aspergillus fumigatus cornea p38 mitogen-activated protein kinase proliferation

Enhanced positive humidity sensitive behavior of p-reduced graphene oxide decorated with n-WS_(2) nanoparticles

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Rare Metals 2021年第7期40卷 1762-1767页

作者： Zai-Hua Duan qiu-Ni zhao Cheng-Zhen Li Si Wang Ya-Dong Jiang Ya-Jie Zhang Bo-Hao Liu Hui-Ling Tai State Key Laboratory of Electronic Thin Films and Integrated Devices School of Optoelectronic Science and EngineeringUniversity of Electronic Science and Technology of ChinaChengdu 610054China

Most resistance-type humidity sensors exhibit negative humidity sensitivity,i.e.,their resistance decreases with a corresponding increase in *** is primarily attributed to the dominant role of ionic conduction in adsorbed *** this work,a humidity sensor based on a p-type reduced graphene oxide(p-rGO)with positive humidity sensitivity is ***,its positive humidity sensing response is further enhanced by n-type WS_(2) nanoparticles *** results show that both rGO and r GO/WS_(2) humidity sensors have good linear response in the relative humidity(RH)range of0%-91.5%.The sensitivity of the rGO/WS_(2) humidity sensor is 1.87 times that of rGO humidity sensor,which is mainly attributed to p-n heterojunction between rGO and WS_(2).Besides,the r GO/WS_(2) humidity sensor has small humidity hysteresis(-3%RH)and good *** work demonstrates a humidity sensor based on rGO/WS_(2) composite film and provides a facile route for fabricating humidity sensor with positive humidity sensing properties.

关键词： Reduced graphene oxide WS2 nanoparticles Heterojunction Humidity sensor Humidity sensing mechanism