Production of interleukin-1β related to mammalian target of rapamycin/Toll-like receptor 4 signaling pathway during Aspergillus fumigatus infection of the mouse cornea

作     者：Rui Xu Jing Lin Gui-Qiu Zhao Cui Li Cheng-Ye Che Qiang Xu Min Liu 

作者机构：Department of Ophthalmologythe Affiliated Hospital of Qingdao University 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2018年第11卷第5期

页      面：712-718页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China(No.81470609 No.81500695) 

主　　题：keratitis interleukin-1β mammalian target of rapamycin Toll-like receptor 4 mice 

摘      要：AIM：To elucidate the effect of rapamycin on regulating the production of interleukin（IL）-1β in Aspergillus fumigatus（***）-induced keratitis and to verify whether the expression of IL-1β in *** keratitis is associated with the mammalian target of rapamycin（mT OR）/Toll-like receptor 4（TLR4） signaling ***：Fungal keratitis mouse models of susceptible C57 BL/6 mice were established using *** mice were subsequently treated with *** protein levels of p-mT OR,TLR4,and IL-1β in normal and infected corneal tissue were measured by Western *** TLR4 and IL-1β m RNA levels were determined by real-time polymerase chain reaction（PCR）.RESULTS：In C57 BL/6 mice,rapamycin treatment decreased the clinical scores and production of the pro-inflammatory cytokine,IL-1β.The expression of TLR4,stimulated by ***,was reduced as well when the mT OR signaling pathway was suppressed by ***：Rapamycin is beneficial for the outcome of fungal keratitis and has an inhibitory effect expression of the inflammatory cytokine IL-1β.The inhibitory effect on IL-1β expression can be associated with the mT OR/TLR4 signaling pathway in *** infection in mice.