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Functional protection of pentoxifylline against spinal cord ischemia/reperfusion injury in rabbits： necrosis and apoptosis effects

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Chinese Medical Journal 2008年第23期121卷 2444-2449页

作者： ZHU Dan-jie XIA bing BI Qing ZHANG Shui-jun qiu bin-song ZHAO Chen Department of Orthopedics Zhejiang Provincial People's Hospital Hangzhou Zhejiang 310014 China

Background Little is known about neuronal death mechanisms following spinal cord ischemia. The present study aimed to investigate the protective effect of pentoxifylline （PTX） against spinal cord ischemia/reperfusion （I/R） injury. Methods Rabbits sustained spinal cord ischemia following 45 minutes cross-clamping of the infrarenal aorta. Experimental groups were as follows： the first group of animals （sham, n=-8） underwent laparotomy alone and served as the sham group; the second group （I/R, n=20） received carrier （3 ml saline solution） and served as the control group; the third group （PTX-A, n=20） received PTX intravenously 10 minutes prior to ischemia; and the fourth group （PTX-B, n=20） received PTX intravenously at the onset of reperfusion. Rabbits were evaluated for hind-limb motor function with the Tarlov scoring system at 48 hours. Serum was assayed with enzyme-linked immunosorbent assay for tumor necrosis factor α （TNF-α） and spinal cords were harvested for myeloperoxidase （MPO） activity, histopathological analysis, terminal deoxynucleotidyl transferase （TdT）-rnediated dUTP nick end labeling staining, platelet/endothelial ceU adhesion molecule-1 （PECAM-1） and caspase-3 immunohistochemistry, and the number of necrotic and apoptotic neuron were counted and data analyzed at 12, 24, 48 and 72 hours of reperfusion. Spinal cords were studied by electron microscopy. Results Improved Tarlov scores were seen in PTX-treated rabbits as compared with ischemic control rabbits at 48 hours. A significant reduction was found in TNF-α in serum, activity of MPO and immunoreactivity of the PECAM-1 and caspase-3 in PTX-treated rabbits. There were fewer apoptotic neurons than necrotic neurons （P 〈0.05）. A significant decrease in both necrotic and apoptotic neurons was observed in the PTX-treated groups （PTX-A and PTX-B） compared with the I/R group （P 〈0.05）. Both necrotic and apoptotic neurons were found with the electron microscope. Conclusions PTX may induce protection aga

关键词： spinal cord injury pentoxifylline necrosis apoptosis

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Effect of combination therapy with alginate dressing and mouse epidermal growth factor on epidermal stem cells in patients with refractory wounds

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Chinese Medical Journal 2012年第2期125卷 257-261页

作者： Bi Qing Zhang Qiong Ma Jun Xu Ming Zhang Shui-jun qiu bin-song Xia bing Gu Hai-feng Hong Jian-fei Zhao Chen Zhu Dan-jie Zhejiang Prov Peoples Hosp Dept Orthoped Hangzhou 310014 Zhejiang Peoples R China Zhejiang Prov Peoples Hosp Dept Operating Room Hangzhou 310014 Zhejiang Peoples R China

Background The aim of this research was to determine the efficacy of combination therapy using an alginate dressing and mouse epidermal growth factor （mEGF） on proliferation and differentiation of epidermal stem cells （ESCs） in patients with refractory *** Eighteen patients （12 males and 6 females,aged from 18 to 61 years （mean 36.4 years）） with various skin wounds,were treated by dressing changing for one *** wounds were located in the foot （11）,calf （3）,thigh （2）and forearm （2）.The patients were randomly divided into 3 groups：alginate dressing and mEGF （group A; n=6）,mEGF （group B; n=6） and control （group C; n=6）.Wound closure indexes were measured at 7,14,21 and 28 *** were harvested for pathologic examination,at 7 and 14 days following *** 10 （CK10） and cytokeratin 15 （CK15） positive cells were evaluated using the super-sensitivity （SP） immunohistochemical staining *** Wound healing was promoted in groups A and *** group A,the wound closure index was increased significantly （P 〈0.05）,and in one case the maximum cure area reached 102 *** examination identified a thicker epidermis,active angiogenesis and enhanced granulation in group A compared with groups B and *** the SP immunohistochemical staining technique,we showed that ESCs in group A were bigger in size and larger in number than in groups B and ***,there was a significant difference in ESCs proliferation and differentiation between group A and group B （or C）.Conclusions Combination therapy using an alginate dressing and mEGF shows increased proliferation and differentiation of ESCs in patients with refractory wounds compared with those treated with mEGF alone.

关键词： alginates epidermal growth factor stem cells wound healing

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关节镜下治疗桡骨茎突狭窄性腱鞘炎的病例对照研究

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中国骨伤 2016年第9期29卷 825-830页

作者：洪剑飞毕擎夏冰章水均邱斌松浙江省人民医院骨科浙江杭州310014

目的 :探讨关节镜下治疗桡骨茎突狭窄性腱鞘炎的临床疗效。方法 :2009年8月至2013年7月收治桡骨茎突狭窄性腱鞘炎患者99例,分为3组:关节镜治疗组(32例,男6例,女26例),外科手术切开腱鞘组(34例,男7例,女27例)和封闭治疗组(33例,男6例,女2... 详细信息

目的 :探讨关节镜下治疗桡骨茎突狭窄性腱鞘炎的临床疗效。方法 :2009年8月至2013年7月收治桡骨茎突狭窄性腱鞘炎患者99例,分为3组:关节镜治疗组(32例,男6例,女26例),外科手术切开腱鞘组(34例,男7例,女27例)和封闭治疗组(33例,男6例,女27例)。观察并比较3组在治疗前、治疗后1周和治疗后1个月腕部及拇指静息状态下疼痛程度、活动状态下疼痛程度、局部压痛程度、局部肿胀情况、腕部及拇指活动情况及总体症状评分方面的差异。同时计算3组在不同时间点的总有效率。结果:随访1周和1个月后,关节镜治疗组和外科手术切开腱鞘组活动疼痛评分低于封闭治疗组(P=0.044和0.039)。1个月后关节镜治疗组和外科手术切开腱鞘组局部压痛评分低于封闭治疗组(P=0.017)。随访1周和1个月后关节镜治疗组和外科手术切开腱鞘组总体症状评分均低于封闭治疗组(P=0.007和0.015)。治疗后随访1周关节镜治疗组、外科手术切开腱鞘组和封闭治疗组总有效率比较,差异无统计学意义(P=0.213),但随访1个月后总有效率差异有统计学意义(P=0.006)。结论:相比传统手术切开腱鞘治疗和封闭治疗,关节镜下治疗桡骨茎突狭窄性腱鞘炎疗效满意,手术创伤小,安全性高,并发症少,在狭窄性腱鞘炎治疗中有一定的优越性。

关键词：腱鞘炎桡骨关节镜病例对照研究

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Potassium and calcium channels in different nerve cells act as therapeutic targets in neurological disorders

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Neural Regeneration Research 2025年第5期20卷 1258-1276页

作者： Qing qiu Mengting Yang Danfeng Gong Haiying Liang Tingting Chen Department of Pharmacology School of PharmacyNantong UniversityNantongJiangsu ProvinceChina Jiangsu Province Key Laboratory of Inflammation and Molecular Drug Target NantongJiangsu ProvinceChina Department of Pharmacy Longyan First Affiliated Hospital of Fujian Medical UniversityLongyanFujian ProvinceChina

The central nervous system, information integration center of the body, is mainly composed of neurons and glial cells. The neuron is one of the most basic and important structural and functional units of the central nervous system, with sensory stimulation and excitation conduction functions. Astrocytes and microglia belong to the glial cell family, which is the main source of cytokines and represents the main defense system of the central nervous system. Nerve cells undergo neurotransmission or gliotransmission, which regulates neuronal activity via the ion channels, receptors, or transporters expressed on nerve cell membranes. Ion channels, composed of large transmembrane proteins, play crucial roles in maintaining nerve cell homeostasis. These channels are also important for control of the membrane potential and in the secretion of neurotransmitters. A variety of cellular functions and life activities, including functional regulation of the central nervous system, the generation and conduction of nerve excitation, the occurrence of receptor potential, heart pulsation, smooth muscle peristalsis, skeletal muscle contraction, and hormone secretion, are closely related to ion channels associated with passive transmembrane transport. Two types of ion channels in the central nervous system, potassium channels and calcium channels, are closely related to various neurological disorders, including Alzheimer's disease, Parkinson's disease, and epilepsy. Accordingly, various drugs that can affect these ion channels have been explored deeply to provide new directions for the treatment of these neurological disorders. In this review, we focus on the functions of potassium and calcium ion channels in different nerve cells and their involvement in neurological disorders such as Parkinson's disease, Alzheimer's disease, depression, epilepsy, autism, and rare disorders. We also describe several clinical drugs that target potassium or calcium channels in nerve cells and could be u

关键词： astrocytes calcium channels central nervous system extracellular ion concentration microglia neurological disorders neurons potassium channels

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Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

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Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： Yi Qi Xueying Chen Bowen Liu Xuliang Lin Xueqing qiu Yanlin Qin Guangdong Provincial Key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic Research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

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Solar trough receiver with V-grooved light traps for efficiency improvement

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Science China Technological Sciences 2025年第1期 146-156页

作者： bin LIU Qing LI Yu qiu School of Energy Science and Engineering Central South University

Parabolic trough collector is a promising technology for both domestic and industrial heat supplies. To enhance the collector efficiency, a modified trough receiver with dozens of V-grooved light traps incorporated into its lower-half absorber was introduced. For assessing the performance of this receiver, a solar-thermal-stress coupled model was established. Using this model, firstly, the effects of the V-grooved light traps were investigated. The results reveal that, compared to the conventional trough receiver, the modified trough receivers equipped with different traps can reduce their optical losses by 81.9–138.0 Wm-1（i.e., 15.0%–25.2%）, improving their optical efficiencies by 1.60%–2.69%. However, these traps also result in increased thermal loss from the receiver. Therefore, there is no single optimal modified trough receiver that consistently outperforms all other receivers across all possible conditions. Nonetheless, the MTR30-3 and MTR60-3 receivers emerge as the optimized choices due to their better solar-thermal performance. Subsequently, performance evaluation reveals that the two optimized receivers can enhance the receiver efficiencies within the typical solar irradiance range by 0.02%–2.65% and 0.05%–1.70% when the inlet fluid temperatures are below 488 and 523 K, respectively. Consequently, the collector efficiencies can be improved by 0.02%–2.49% and 0.05%–1.60%, respectively. Additionally, thermal stress analysis verifies that the two optimized receivers remain within safe limits under all typical conditions. Given that the heating temperature of a typical solar collector for domestic or industrial heat supply is typically below 523 K, the integration of the two optimized receivers has the capability to effectively enhance the overall collector efficiency.

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己酮可可碱对兔缺血再灌注损伤脊髓的保护作用及机制

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中国病理生理杂志 2008年第5期24卷 950-954页

作者：朱丹杰毕擎夏冰洪剑飞邱斌松章水均浙江省人民医院骨科浙江杭州310014

目的:对己酮可可碱(PTX)在脊髓缺血再灌注损伤中神经元的保护作用及其机制进行初步探讨。方法:采用日本大耳白兔腹主动脉夹闭法建立脊髓缺血再灌注损伤动物模型,随机分为A组(假手术组,8只)、B组(对照组,20只)、C组(夹闭血管前用药组,20... 详细信息

目的:对己酮可可碱(PTX)在脊髓缺血再灌注损伤中神经元的保护作用及其机制进行初步探讨。方法:采用日本大耳白兔腹主动脉夹闭法建立脊髓缺血再灌注损伤动物模型,随机分为A组(假手术组,8只)、B组(对照组,20只)、C组(夹闭血管前用药组,20只)、D组(再灌注即刻用药组,20只)。于再灌注后12h、24h、48h、72h检测血TNF-α活性、组织MPO活性、免疫组化法观察并检测PECAM-1及caspase-3表达、HE染色观察神经元并计数坏死神经元、TUNEL染色观察并计数凋亡神经元、电镜观察坏死及凋亡神经元形态改变,并于再灌注后48h进行运动功能评分(改良Tarlov评分)。结果:用药组(C组及D组)改良Tarlov评分明显高于对照组(P<0.05),血TNF-α、组织MPO含量、免疫组化PECAM-1及caspase-3表达强度均明显下降,HE及TUNEL染色切片中坏死细胞及凋亡细胞均明显减少,与对照组有显著差异(P<0.05)。假手术组未见坏死及凋亡细胞。结论:己酮可可碱在脊髓缺血再灌注损伤中能够发挥抑制神经元坏死及凋亡的双重脊髓保护作用。

关键词：再灌注损伤己酮可可碱脊髓兔神经元

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关节镜下运用4股腘绳肌腱同期重建前后交叉韧带损伤

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中华创伤杂志 2007年第8期23卷 597-601页

作者：毕擎朱丹杰夏冰洪剑飞邱斌松章水均浙江省人民医院关节外科中心杭州310014

目的关节镜下运用Intrafix和可吸收界面螺钉固定自体4股腘绳肌腱，同期重建前交叉韧带（anterior cruciate ligament，ACL）、后交叉韧带（posterior cruciate ligament，PCL），评估其疗效。方法 ACL、PCL同时损伤的患者16例，关节镜... 详细信息

目的关节镜下运用Intrafix和可吸收界面螺钉固定自体4股腘绳肌腱，同期重建前交叉韧带（anterior cruciate ligament，ACL）、后交叉韧带（posterior cruciate ligament，PCL），评估其疗效。方法 ACL、PCL同时损伤的患者16例，关节镜下以自体4股胭绳肌腱作为重建移植物，应用可吸收界面螺钉固定移植物股骨端，Intrafix钉鞘和可吸收锥形钉固定胫骨端，同期行ACL和PCL损伤重建术。7例行内侧副韧带修补，4例行外侧副韧带复合结构修复，2例行内外侧同时修复。所有患者按照国际膝关节评分委员会（International Knee Documentation Committee，IKDC）评分标准进行术前评估，均为D级。术前Lysholm评分为（36．5±3．7）分。结果随访时间为12～18个月，平均14．6个月。终末随访时，IKDC总体评价：A级6例（38％），B级9例（56％），C级1例（6％），无D级患者。Lachman试验0～2mm8例；3～5mm6例；6～10mm2例（P〈0．05）。屈70°前后总位移0～2mm10例；3～5mm5例；6～10mm1例（P〈0．05）。屈70°后位移0～2mm12例；3～5mm4例（P〈0．05）。术后2个月Lysholm功能评分为（90．4±2．9）分，终末随访时为（93．4±3．5）分，与术前相比差异均有统计学意义（P〈0．05）。结论关节镜下以自体4股胭绳肌腱作为移植物，应用可吸收界面螺钉、Intrafix钉鞘和可吸收锥形钉固定股骨胫骨端同期重建ACL和PCL损伤，有利于早期积极的功能康复，膝关节功能恢复满意。

关键词：膝关节膝损伤前交叉韧带后交叉韧带关节镜检查

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膝关节前交叉韧带重建的研究现状

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中国骨伤 2021年第12期34卷 1091-1094页

作者：毕擎陈航赵晨邱斌松顾海峰曹力柴昉浙江省人民医院骨科浙江杭州310014

前交叉韧带(anterior cruciate ligament,ACL)是防止胫骨前移的基本结构,对阻止胫骨内旋也有特殊作用,其损伤后会引起膝关节前后向及旋转不稳定。近年来ACL损伤已成为最常见的运动损伤之一,关节镜下手术重建是其首选治疗手段。随着运动... 详细信息

前交叉韧带(anterior cruciate ligament,ACL)是防止胫骨前移的基本结构,对阻止胫骨内旋也有特殊作用,其损伤后会引起膝关节前后向及旋转不稳定。近年来ACL损伤已成为最常见的运动损伤之一,关节镜下手术重建是其首选治疗手段。随着运动医学的理念及关节镜技术的不断发展,ACL重建的手术理念也在不断地演化,从事运动医学的医生一直致力于寻找最佳的重建方式和技术。本文基于近些年来的相关研究,针对ACL的解剖、骨隧道定位、移植物选择与固定以及生物学制剂等方面的情况作一述评。

关键词：前交叉韧带外科手术关节镜

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AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

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Neural Regeneration Research 2025年第8期20卷 2408-2419页

作者： Ruiqi qiu Mingzhu Yang Xiuxiu Jin Jingyang Liu Weiping Wang Xiaoli Zhang Jinfeng Han Bo Lei Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Eye Institute Henan Academy of Innovations in Medical ScienceZhengzhouHenan ProvinceChina Branch of National Clinical Research Center for Ocular Disease Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina Academy of Medical Science Zhengzhou UniversityZhengzhouHenan ProvinceChina

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell ***,there is no effective treatment for retinitis pigmentosa caused by PDE6B ***-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis *** aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal *** do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and ***-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western ***2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 *** expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type *** Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction ***,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B ***,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B *** data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upr

关键词： apoptosis AAV2-PDE6B ERK1/2 gene therapy phototransduction proteomics rd10 retinitis pigmentosa

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