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Journal of Pharmaceutical Analysis 2022年第6期12卷 913-922页

作者： Liu Liu Juncheng Hong Wenhai wang Shu Xiao Hongzhen Xie qiqin wang Ning Gan School of Material Science and Chemical Engineering Ningbo UniversityNingboZhejiang315211China Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China

In this study,a fluorescent(FL)aptasensor was developed for on-site detection of live Salmonella typhimurium(S.T.)and Vibrio parahaemolyticus(V.P.).Complementary DNA(cDNA)of aptamer(Apt)-functionalized multicolor polyhedral oligomeric silsesquioxane-perovskite quantum dots(cDNA-POSSPQDs)were used as encoded probes and combined with dual-stirring-bar-assisted signal amplification for pathogen *** this system,bar 1 was labeled with the *** ***,and then bar 2 was functionalized with *** *** *** introduced,pathogen-Apt complexes would form and be released into the supernatant from bar *** agitation,the two complexes reached bar 2 and subsequently reacted with cDNA-POSS-PQDs,which were immobilized on ***,the encoded probes would be detached from bar 2 to generate FL signals in the ***,the pathogens can resume their free state and initiate next *** swim between the two bars,and the FL signals can be gradually enhanced to maximum after several *** FL signals from released encoded probes can be used to detect the *** particular,live pathogens can be distinguished from dead ones by using an *** detection limits and linear range for *** *** 30 and 10 CFU/mL and 10^(2) -10^(6) CFU/mL,***,this assay has broad application potential for simultaneous on-site detection of various live pathogenic bacteria in water.

关键词： Aptasensor Live bacteria Multiple detection Multicolor perovskite quantum dots Dual-stirring-bar

Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography

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Journal of Pharmaceutical Analysis 2022年第2期12卷 332-338页

作者： Peijie Zhu Weijia Chen qiqin wang Huihui Wu Meng Ruan Hongwu wang Zhengjin Jiang School of Food&Pharmaceutical Engineering Zhaoqing UniversityZhaoqingGuangdong526061China Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China School of Pharmaceutical Engineering Guangdong Food and Drug Vocational CollegeGuangzhou510632China Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research Jinan UniversityGuangzhou510632China

In this research,a new phospholipid based monolith was fabricated by in situ co-polymerization of 1-dodecanoyl-2-(11-methacrylamidoundecanoyl)-sn-glycero-3-phosphoethanolamine and ethylene dimethacrylate to mimick bio-membrane *** physicochemical properties of this novel monolith that were achieved included column efficiency,stability,and ***,the biomimetic monolith showed outstanding separation capability for a series of intact proteins and small *** particular,it exhibited good potential as an alternative to the commercial immobilized artificial membrane(IAM)column(***2)for studying drug-membrane *** study not only enriched the types of IAM stationary phases,but also provided a simple model for the prediction of phosphatidylethanolamine related properties of drug candidates.

关键词： Phosphatidylethanolamine Biomimetic monolith IAM Drug-membrane interaction

Determination of L-norvaline and L-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11- dihydroquinidine-silica hybrid monolithic column

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Journal of Pharmaceutical Analysis 2020年第1期10卷 70-77页

作者： Dongsheng Xu Elena Sanchez-Lopez qiqin wang Zhengjin Jiang María Luisa Marina Departamento de Química Analítica Química Física e Ingeniería QuímicaUniversidad de AlcalaCtra.Madrid-Barcelona Km.33.60028871Alcala de Henares(Madrid)Spain Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research Jinan UniversityGuangzhou510632China Instituto de Investigacion Química“Andres M.del Río”(IQAR) Universidad de AlcalaCtra.Madrid-Barcelona Km.33.60028871Alcala de Henares(Madrid)Spain

An analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine(MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl(FMOC)derivatized amino acids by nano-liquid *** mobile phase was optimized including the apparent pH,content of ACN,and concentration of the buffer to obtain a satisfactory enantioresolution performance.27 FMOC derivatized amino acids including 19 protein and 8 non-protein amino acids were tested,and 19 out of them were enantiomerically discriminated obtaining baseline separation for 11 of *** characteristics of the method were evaluated for norvaline and tryptophan in terms of linearity,precision,accuracy,limits of detection(LOD)and quantitation(LOQ)showing good performance to be applied to the enantiomeric determination of these amino acids in dietary *** and LOQ values were 9.3 and 31 mM for norvaline enantiomers and 7.5 and 25 mM for tryptophan enantiomers,*** contents of D-norvaline and D-tryptophan were below their respective LODs in all the analyzed *** of L-tryptophan and L-norvaline showed good agreement with the labeled contents except for one sample which did not show presence of L-norvaline,contrary to the label indication.

关键词： 9-Fluorenylmethoxycarbonyl Amino acids Chiral separation Nano-LC Quinidine-silica hybrid monolithic column

Mimotope peptide modified pompon mum-like magnetic microparticles for precise recognition,capture and biotransformation analysis of rituximab in biological fluids

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1317-1328页

作者： Jiawen Yang Aixuan Zhou Minyi Li Qiaoxian He Jingwei Zhou Jacques Crommen Wentao wang Zhengjin Jiang qiqin wang Institute of Pharmaceutical Analysis College of Pharmacy/State Key Laboratory of Bioactive Molecules and Druggability Assessment/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of ChinaJinan UniversityGuangzhou 510632China Laboratory for the Analysis of Medicines Department of Pharmaceutical SciencesCIRMUniversity of LiegeLiege B-4000Belgium SCIEX Shanghai 200050China

Due to low immobilized ligand density,limited binding capacity,and severe interference from serum proteins,developing ideal peptide-based biomaterials for precise recognition and in vivo analysis of biopharmaceuticals remains a huge *** this study,mimotope peptide modified pompon mum-like biomimetic magnetic microparticles(MMPs,3.8μm)that mimic the specific functionalities of CD20 on malignant B cells were developed for the first *** from the numerous ligand binding sites(Ni^(2+))on the pompon mum-like MMPs,these novel materials achieved≥10 times higher peptide ligand densities(>2300 mg/g)and antibody binding capacities(1380 mg/g)compared to previous reported *** the high specificity of the mimotope peptide,rituximab can be precisely recognized and enriched from cell culture media or serum *** also established an LC-MS/MS method using the MMPs for tracking rituximab biotransformation in patient ***,deamidation of Asn55 and Asn33,as well as oxidation of Met81 and Met34 were observed at the key complementarity determining regions of rituximab,which could potentially influence antibody function and require careful ***,these versatile biomimetic MMPs demonstrate superior recognition and enrichment capabilities for target antibodies,offering interesting possibilities for biotransformation analysis of biopharmaceuticals in patient serum.

关键词： Therapeutic monoclonal antibody Mimotope peptide Precise recognition Peptide-based biomaterials Biotransformation Patient serum

Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography

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Journal of Pharmaceutical Analysis 2022年第5期12卷 783-790页

作者： qiqin wang Lingjue Sun Huihui Wu Ning Deng Xianglong Zhao Jingwei Zhou Tingting Zhang Hai Han Zhengjin Jiang Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China Anhui Prevention and Treatment Center for Occupational Disease Anhui No.2 Provincial People's HospitalHefei230041China Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education Lab of BiochemistryCollege of ChemistryXiangtan UniversityXiangtanHunan411105China Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research Jinan UniversityGuangzhou510632China

Zwitterionic sulfobetaine-based monolithic stationary phases have attracted increasing attention for their use in hydrophilic interaction *** this study,a novel hydrophilic polymeric monolith was fabricated through photo-initiated copolymerization of 3-(3-vinyl-1-imidazolio)-1-propanesulfonate(SBVI)with pentaerythritol triacrylate using methanol and tetrahydrofuran as the porogenic ***,the duration for the preparation of this novel monolith was as little as 5 min,which was significantly shorter than that required for previously reported sulfobetaine-based monoliths prepared via conventional thermally initiated ***,these monoliths showed good morphology,permeability,porosity(62.4%),mechanical strength(over 15 MPa),column efficiency(51,230 plates/m),and reproducibility(relative standard deviations for all analytes were lower than 4.6%).Mechanistic studies indicated that strong hydrophilic and negative electrostatic interactions might be responsible for the retention of polar analytes on the zwitterionic SBVI-based *** particular,the resulting monolith exhibited good anti-protein adhesion ability and low nonspecific protein *** excellent features seem to favor its application in ***,the novel zwitterionic sulfobetaine-based monolith was successfully employed for the highly selective separation of small bioactive compounds and the efficient enrichment of N-glycopeptides from complex *** this study,we prepared a novel zwitterionic sulfobetaine-based monolith with good performance and developed a simpler and faster method for preparation of zwitterionic monoliths.

关键词： Zwitterionic monolith Sulfobetaine Photo-initiated copolymerization Hydrophilic interaction chromatography Complex sample

Multiepitope recognition technology promotes the in-depth analysis of antibody‒drug conjugates

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Acta Pharmaceutica Sinica B 2024年第11期14卷 4962-4976页

作者： Yutian Lei Yuan Shen Feng Chen Rui He Zhang Zhang Ying Zhou Jin-Chen Yu Jacques Crommen Zhengjin Jiang qiqin wang Institute of Pharmaceutical Analysis College of Pharmacy/State Key Laboratory of Bioactive Molecules and Druggability Assessment/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of ChinaJinan UniversityGuangzhou 510632China School of Biomedical Engineering Sun Yat-sen UniversityShenzhen 518107China Department of Clinical Pharmacy Wuhan Children's Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430016China Bio-Thera Solutions LtdGuangzhou 510700China Laboratory for the Analysis of Medicines Department of Pharmaceutical SciencesCIRMUniversity of LiegeLiege B-4000Belgium

The dynamic tracking of antibody‒drug conjugates (ADCs) in serum is crucial. However, a versatile bioanalytical platform is lacking due to serious matrix interferences, the heterogeneity and complex biotransformation of ADCs, and the recognition deficiencies of traditional affinity technologies. To overcome this, a multiepitope recognition technology (MERT) was developed by simultaneously immobilizing CDR and non-CDR ligands onto MOF@AuNPs. MERT's excellent specificity, ultrahigh ligand density, and potential synergistic recognition ability enable it to target the different key regions of ADCs to overcome the deficiencies of traditional technologies. The binding capacity of MERT for antibodies is ten to hundred times higher than that of the mono-epitope or Fc-specific affinity technologies. Since MERT can efficiently capture target ADCs from serum, a novel bioanalytical platform based on MERT and RPLC‒QTOF-MS has been developed to monitor the dynamic changes of ADCs in serum, including the fast changes of drug-to-antibody ratio from 3.67 to 0.22, the loss of payloads (maytansinol), and the unexpected hydrolysis of the succinimide ring of the linker, which will contribute to clarify the fate of ADCs and provide a theoretical basis for future design. In summary, the MERT-based versatile platform will open a new avenue for in-depth studies of ADCs in biological fluids.

关键词： Antibody-drugconjugate Multiepitoperecognition strategy Affinityenrichment Biotransformation LC-MS

Synthesis of organic nitrates of apigenin as a novel class of potent alpha-glucosidase inhibitors

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Synthesis of organic nitrates of apigenin as a novel class o...

考虑双重声誉的城市轨道交通服务水平激励模型构建研究

第八届全国化学生物学学术会议

作者： qiqin wang Ning Cheng Wenbing Yi Yi Chen Shengming Peng Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education College of ChemistryXiangtan University

＜正＞α-Glucosidase（AG） plays crucial roles in the digestion of carbohydrates.α-glucosidase inhibitors（AGIs）have received significant attentions as potent therapeutic drugs for diabetic *** on combination principles,two series of apigenin nitric oxide （NO）-donating derivatives were synthesised and evaluated for their AG inhibitory activity and NO

关键词： Apigenin derivatives Chrysin derivatives α-glucosidase inhibitor NO donor

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铁道运输与经济 2020年第12期42卷 99-105页

作者：陈群汪琦钦陈哲福建工程学院管理学院福建福州350118 宁德职业技术学院福建宁德355099

城市轨道交通不仅是缓解交通拥堵的重要工具,更是重塑城市空间结构、输出城市优秀文化的有力手段。通过分析城市出行者、政府、城市轨道交通企业三者之间的需求与联系,构建考虑双重声誉的城市轨道交通项目服务水平激励博弈模型,并以福... 详细信息

城市轨道交通不仅是缓解交通拥堵的重要工具,更是重塑城市空间结构、输出城市优秀文化的有力手段。通过分析城市出行者、政府、城市轨道交通企业三者之间的需求与联系,构建考虑双重声誉的城市轨道交通项目服务水平激励博弈模型,并以福州地铁为例,与仅考虑乘客印象中声誉的激励博弈模型进行比较分析。研究表明:通过引入政府印象中的声誉激励有利于提高企业服务水平努力程度,且当声誉激励高时,风险厌恶特征对企业服务水平努力程度影响较小;2种模型中,乘客印象中的声誉对企业的服务水平努力程度具有正向影响;贴现因子系数越大,即政府对企业服务水平的评估周期越短,则越有利于提高城市轨道交通企业的服务水平努力程度。

关键词：城市轨道交通服务水平双重声誉激励机制激励博弈模型

城市轨道交通运营安全应急能力水平提升的激励研究

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铁道运输与经济 2023年第5期45卷 149-156页

作者：陈群田昌民陈哲汪琦钦福建工程学院管理学院福建福州350118 宁德职业技术学院福建宁德355000 中建海峡建设发展有限公司华东分公司上海201700

为了提升运营企业对安全应急能力持续提升的投入动力,保证城市轨道交通能够在日益复杂多变的环境中应对新的风险挑战,从企业的安全社会责任意识内生动力角度考虑,构建基于城市轨道交通运营安全应急能力提升的激励模型,并以福州地铁为例... 详细信息

为了提升运营企业对安全应急能力持续提升的投入动力,保证城市轨道交通能够在日益复杂多变的环境中应对新的风险挑战,从企业的安全社会责任意识内生动力角度考虑,构建基于城市轨道交通运营安全应急能力提升的激励模型,并以福州地铁为例进行仿真计算分析。结果表明:在考虑安全社会责任意识的内生动力情况下,运营企业的安全社会责任度与运营企业的安全应急能力提升的努力程度成正比;当安全社会责任度较大时,运营企业的应急风险偏好表现为风险喜好型,其提升应急能力的努力程度与绝对风险规避度成正比,企业表现为对主动提升应急能力的主观意愿越来越强,对敢于承担应急风险的积极性越高,且对积极控制事故的发展态势具有促进作用,减少事故损失。

关键词：城市轨道交通应急能力安全社会责任意识政府激励委托代理理论

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磷霉素钠172例临床应用分析

中国现代医药杂志 2008年第2期10卷 45-46页

作者：黄殷王启琴广西北海市人民医院

目的了解我院注射用磷霉素钠的临床使用情况,为合理使用该药提供依据。方法回顾分析我院2007年1￣11月172份住院患者使用磷霉素钠的临床资料,根据磷霉素钠的用法、用量、适应性、联合用药等分析该药使用是否正确。结果172份病历中,磷霉... 详细信息

目的了解我院注射用磷霉素钠的临床使用情况,为合理使用该药提供依据。方法回顾分析我院2007年1￣11月172份住院患者使用磷霉素钠的临床资料,根据磷霉素钠的用法、用量、适应性、联合用药等分析该药使用是否正确。结果172份病历中,磷霉素钠单一用药占50.00%,二联用药占45.34%,三联用药占4.65%;与注射用磷霉素钠联合用药的抗菌药物构成比为,青霉素类占25.28%,喹诺酮类占23.26%,头孢类占20.93%,β-酶抑制剂类占16.28%,氨基糖苷类占13.95%;用药时间构成比为,1￣3天占40.70%,4￣6天占32.60%,7￣9天占12.79%,9天以上占13.95%;临床应用构成比为,治疗用药110例,占64%,预防用药62例,占36%。结论各科室对磷霉素钠的使用基本正确,但存在用药起点过高,使用时间过长,不必要的联合用药,不作病原学检查等问题。

关键词：磷霉素钠合理用药注射临床使用联合用药