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Screening and cloning for proteins transactivated by the PS1TP5 protein of hepatitis B virus:A suppression subtractive hybridization study

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World Journal of Gastroenterology 2007年第10期13卷 1602-1607页

作者： Jian-Kang zhang Long-Feng Zhao Jun Cheng Jiang Guo Dan-qiong Wang yuan Hong yu Mao Department of Gastroenterology The First Hospital of Shanxi Medical University Taiyuan 030001 Shanxi Province China Institute of Infectious Diseases Beijing Ditan HospitalBeijing 100011 China

AIM： To clone and identify human genes transactivated by PSITP5 by constructing a cDNA subtractive library with suppression subtractive hybridization （SSH） technique. METHODS： SSH and bioinformatics techniques were used for screening and cloning of the target genes transactivated by PS1TP5 protein. The mRNA was isolated from HepG2 cells transfected with pcDNA3.1（-）- myc-his（A）-PS1TP5 and pcDNA3.1（-）-myc-his（A） empty vector, respectively, and SSH technique was employed to analyze the differentially expressed DNA sequence between the two groups. After digestion with restriction enzyme Rsa Ⅰ, small size cDNAs were obtained. Then tester cDNA was divided into two groups and ligated to the specific adaptor 1 and adaptor 2, respectively. The tester cDNA was hybridized with driver cDNA twice and subjected to nested PCR for two times, and then subcloned into T/A plasmid vectors to set up the subb-active library. Amplification of the library was carried out with E.. coil strain DH5α. The cDNA was sequenced and analyzed in GenBank with Vector NTI 9.1 and NCBI BLAST software after PCR amplification. RESULTS： The subtractive library of genes transactivated by PS1TP5 was constructed successfully. The amplified library contained 90 positive clones. Colony PCR showed that 70 clones contained 200-1000-bp inserts. Sequence analysis was performed in 30 clones randomly, and the full-length sequences were obtained by bioinformatics technique. Altogether 24 coding sequences were obtained, which consisted of 23 known and 1 *** novel gene with unknown functions was found and named as PSITP5TP1 after being electronically spliced, and deposited in GenBank （accession number： DQ487761）. CONCLUSION： PSITP5 is closely correlated with immunoregulation, carbohydrate metabolism, signal transduction, formation mechanism of hepatic fibrosis, and occurrence and development of tumor. Understanding PSlTP5 transactive proteins may help to bring some new clues for further studying the biological f

关键词： Hepatitis B virus Pre-S1 protein Suppression subtractive hybridization

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Chemical and optical properties of aerosols and their interrelationship in winter in the megacity Shanghai of China

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Journal of Environmental Sciences 2015年第1期27卷 59-69页

作者： Tingting Han Liping Qiao Min Zhou yu Qu Jianfei Du Xingang Liu Shengrong Lou Changhong Chen Hongli Wang Fang zhang Qing yu qiong Wu State Key Laboratory of Water Environment Simulation School of Environment Beijing Normal University State Environmental Protection Key Laboratory of Cause and Prevention of Urban Air Pollution Complex Shanghai Academy of Environmental Sciences State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of Sciences Shanghai Meteorological Service College of Global Change and Earth System Science Beijing Normal University

A field campaign on air quality was carried out in Shanghai in winter of 2012. The concentrations of NO, NO2, NOx, SO2, CO, and PM2.5increased during haze formation. The average masses of SO4^2-, NO3^-and NH4^＋were 10.3, 11.7 and 6.7 μg/m^3 during the haze episodes, which exceeded the average（9.2, 7.9, and 3.4 μg/m3） of these components in the non-haze days. The mean values for the aerosol scattering coefficient（b sp）, aerosol absorption coefficient（b ap） and single scattering albedo（SSA） were 288.7, 27.7 and0.91 Mm-1, respectively. A bi-peak distribution was observed for the mass concentrations of CO, NO, NO2, and NOx. More sulfate was produced during daytime than that in the evening due to photochemical reactions. The mass concentration of NH4＋achieved a small peak at noontime. NO3-showed lower concentrations in the afternoon and higher concentrations in the early morning. There were obvious bi-peak diurnal patterns for b sp and b ap as well as SSA. b sp and b ap showed a positive correlation with PM2.5mass concentration.（NH4）2SO4, NH4NO3, organic mass, elemental carbon and coarse mass accounted for 21.7%, 19.3%, 31.0%, 9.3% and 12.3% of the total extinction coefficient during non-haze days, and 25.6%, 24.3%, 30.1%, 8.1% and 8.2% during hazy days. Organic matter was the largest contributor to light extinction. The contribution proportions of ammonium sulfate and ammonium nitrate to light extinction were significantly higher during the hazy time than during the non-haze days.

关键词： Optical properties Diurnal variations Chemical apportionment Shanghai

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Characteristics of inorganic phosphorus fractions and their correlations with soil properties in three non-acidic soils

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Journal of Integrative Agriculture 2022年第12期21卷 3626-3636页

作者： zhang Nai-yu WANG qiong ZHAN Xiao-ying WU Qi-hua HUANG Shao-min ZHU Ping YANG Xue-yun zhang Shu-xiang Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences/National Engineering Laboratory for Improving Quality of Arable LandBeijing 100081P.R.China TERRA Gembloux Agro-Bio TechUniversity of LiegeGembloux 5030Belgium Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Bioengineering Guangdong Academy of Sciences/Guangdong Modern Agricultural Technology Research and Development CenterGuangzhou 510316P.R.China Institute of Plant Nutrient Agricultural Resources and Environmental SciencesHenan Academy of Agricultural SciencesZhengzhou 450002P.R.China Institute of Agricultural Resources and Environment Jilin Academy of Agricultural SciencesChangchun 130033P.R.China College of Natural Resources and Environment Northwest A&F UniversityYangling 712100P.R.China

Understanding the characteristics and influences of various factors on phosphorus(P)fractions is of significance for promoting the efficiency of soil *** on long-term experiments on black soil,fluvo-aquic soil,and loess soil,which belong to Phaeozems,Cambisols,and Anthrosols in the World Reference Base for Soil Resources(WRB),respectively,five fertilization practices were selected and divided into three groups:no P fertilizer(CK/NK),balanced fertilizer(NPK/NPKS),and manure plus mineral fertilizer(NPKM).Soil inorganic P(Pi)fractions and soil properties were analyzed to investigate the characteristics of the Pi fractions and the relationships between Pi fractions and various soil *** results showed that the proportion of Ca_(10)-P in the sum of total Pi fractions was the highest in the three soils,accounting for 33.5%in black soil,48.8%in fluvo-aquic soil,and 44.8%in loess ***-term fertilization practices resulted in periodic changes in soil Pi accumulation or *** black soil and fluvo-aquic soil,the Pi accumulation was higher in the late period(10–20 years)of fertilization than in the early period(0–10 years)under NPK/NPKS and NPKM,whereas the opposite result was found in loess *** Pi accumulation occurred in all Pi fractions in black soil;mainly in Ca_(8)-P,Fe-P,and Ca_(10)-P in fluvo-aquic soil;and in Ca_(2)-P,Ca_(8)-P,and O-P in loess *** CK/NK,the soil Pi was depleted mainly in the early period in each of the three *** addition to the labile Pi(Ca_(2)-P)and moderately labile Pi(Ca_(8)-P,Fe-P,Al-P),the Ca_(10)-P in black soil and fluvo-aquic soil and O-P in loess soil could also be used by *** analysis showed that soil properties explained more than 90%of the variation in the Pi fractions in each soil,and the explanatory percentages of soil organic matter(SOM)were 43.6%in black soil,74.6%in fluvo-aquic,and 38.2%in loess ***,decisions regarding the application of P fertilizer should consider

关键词： non-acidic soils long-term fertilization phosphorus fractions soil properties organic matter

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Epigenetics of hepatocellular carcinoma:Role of microRNA

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World Journal of Gastroenterology 2013年第33期19卷 5439-5445页

作者： Sharad Khare qiong zhang Jamal A Ibdah Division of Gastroenterology and Hepatology Department of Internal MedicineUniversity of Missouri Harry S Truman Memorial Veterans’ Hospital

Hepatocellular carcinoma(HCC)represents a major form of primary liver cancer in ***(miRs),small non-coding single-stranded RNAs of 19-24nucleotides in length,negatively regulate the expression of many target genes at the post-transcriptional and/or translational levels and play a critical role in the initiation and progression of *** this review we have summarized the information of aberrantly expressed miRs in HCC,their mechanism of action and relationship to *** recent advances in HCC research reveal that miRs regulate expression of various oncogenes and tumor suppressor genes,thereby contributing to the modulation of diverse biological processes including proliferation,apoptosis,epithelial to mesenchymal transition and *** a clinical viewpoint,polymorphisms within miR-binding sites are associated with the risk of *** in miR related genes have been shown to correlate with survival or treatment outcome in ***,the review focuses on the potential role of miRs as novel biomarkers and their translational applications for diagnosis and therapy in *** further insights into miR deregulation in HCC,it is expected that novel miR-based therapeutics will ***,we orient the readers to other reviews that may provide better understanding of miR research in HCC.

关键词： MicroRNA Cancer Hepatocellular carcinoma Biomarker Polymorphism

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Prediction of the high-quality development level of inbound tourism based on adaptive neural network technology

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Journal of Control and Decision 2023年第1期10卷 112-125页

作者： Hongxi zhang Wei Wei qiong Liu Department of Tourism Management South China University of TechnologyGuangzhouPeople’s Republic of China Tourism and Air Service College Guizhou Minzu UniversityGuiyangPeople’s Republic of China Beijing Guanghuijintong Education and Technology Co.Ltd GuiyangPeople’s Republic of China

Visitors abroad come on an inbound tourism (IT) trip. Travelling beyond one’s usual surroundingsfor no more than a year in one go, or no less than tourist culture is considered inboundtourism. A network called adaptive neural networks (ANN) is used in dynamic tourism *** methods are used to make neural networks flexible: weight adjustment, changes in neuronalcharacteristics, and changes in the network’s structural composition. One of the biggestproblems is that cultural clashes lead to many different places, which strain the transportationsystem. IT-ANN tools and technologies are discussed in this study, including the possibilities ofvisitor time and space-relationship data. As a result, inbound tourism doesn’t have to rely onweekend domestic travel because individuals who go on holiday overseas tend to return yearafter year. Finally, the neural network is equipped with a prediction model of travellers’ spatialand temporal distribution.

关键词： Neural networks inbound tourist tourism culture transport network

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The Cleavage Effect of Histidyl-Lysine on RNA

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Chinese Chemical Letters 1998年第4期9卷 333-334页

作者： Xiang Hong LI Rong WAN qiong zhang yu Fen ZHAO(Department of Chemistry, Tsinghua University, Bejing 100084) Department of Chemistry Tsinghua University Beijing 100084 China

The cleavage activity of histidyl-lysine dipeptide (His-Lys) on RNA was studied and compared with that of histidine. With the addition of the lysine residue, the cleavage activity was increased about 10-fold compared ... 详细信息

关键词： RNA cleavage histidyl-lysine

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Ecdysone receptor isoforms play distinct roles in larval–pupal–adult transition in Leptinotarsa decemlineata

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Insect Science 2020年第3期27卷 487-499页

作者： Qing-yu Xu Pan Deng qiong zhang Ang Li Kai-yun Fu Wen-Chao Guo Guo-Qing Li Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests College of Plant ProtectionNanjing Agricultural UniversityNanjingChina Institute of Plant Protection Xinjiang Academy of Agricultural SciencesUrumqiChina Institute of Microbiological Application Xinjiang Academy of Agricultural ScienceUrumqiChina

A heterodimer of two nuclear receptors,ecdysone receptor(EcR)and ultraspiracle,mediates 20‐hydroxyecdysone(20E)signaling to modulate many aspects in insect life,such as molting and metamorphosis,reproduction,diapause and innate *** the present paper,we intended to determine the isoform‐specific roles of EcR during larval–pupal–adult transition in the Colorado potato ***‐stranded RNAs(dsRNAs)were prepared using the common(dsEcR)or isoform‐specific(dsEcRA,dsEcRB1)regions of EcR as *** of either dsEcR or dsEcRA,rather than dsEcRB1,by the penultimate(3rd)and final(4th)instar larvae caused failure of larval–pupal and pupal–adult *** RNA interference(RNAi)larvae remained as prepupae,or became deformed pupae and *** of messenger RNA(mRNA)levels of EcR isoforms found that LdEcRA regulates the expression of ***,silencing the two EcR transcripts,LdEcRA or LdEcRB1 reduced the mRNA levels of Ldspo and Ldsad,and lowered 20E *** contrast,the expression levels of HR3,HR4,E74 and E75 were significantly decreased in the LdEcR or LdEcRA RNAi larvae,but not in LdEcRB1 depleted *** supplement with 20E did not restore the expression of five 20E signaling genes(USP,HR3,HR4,E74 and E75),and only partially alleviated the pupation defects in dsEcR‐or dsEcRA‐fed *** data suggest that EcR plays isoform‐specific roles in the regulation of ecdysteroidogenesis and the transduction of 20E signal in ***.

关键词： ecdysone receptor Leptinotarsa decemlineata metamorphosis RNA interference

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Elicitor hydrophobin Hyd1 interacts with Ubiquilin1-like to induce maize systemic resistance

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Journal of Integrative Plant Biology 2020年第4期62卷 509-526页

作者： Chuanjin yu Kai Dou Shaoqing Wang qiong Wu Mi Ni Tailong zhang Zhixiang Lu Jun Tang Jie Chen Key Laboratory of Urban Agriculture(South)Ministry of Agriculture School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai 200240China School of Life Science Fuyang Normal UniversityFuyang 236037China

Trichoderma harzianum is a plant-beneficial fungus that secretes small cysteine-rich proteins that induce plant defense responses;however, the molecular mechanism involved in this induction is largely ***, we report that the class Ⅱ hydrophobin Th Hyd1 acts as an elicitor of induced systemic resistance(ISR) in plants. Immunogold labeling and immunofluorescence revealed Th Hyd1 localized on maize(Zea mays) root cell plasma membranes. To identify host plant protein interactors of Hyd1, we screened a maize B73 root c DNA library. Th Hyd1 interacted directly with ubiquilin1-like(UBL). Furthermore, the N-terminal fragment of UBL was primarily responsible for binding with Hyd1 and the eight-cysteine amino acid of Hyd1 participated in the protein-protein interactions. Hyd1 from T. harzianum(Thhyd1) and ubl from maize were co-expressed in Arabidopsis thaliana, they synergistically promoted plant resistance against Botrytis cinerea. RNA-sequencing analysis of global gene expression in maize leaves 24 h after spraying with Curvularia lunata spore suspension showed that Thhyd1-induced systemic resistance was primarily associated with brassinosteroid signaling, likely mediated through BAK1. Jasmonate/ethylene(JA/ET)signaling was also involved to some extent in this response. Our results suggest that the Hyd1-UBL axis might play a key role in inducing systemic resistance as a result of Trichoderma-plant interactions.

关键词： Zea mays resistance maize

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元启发式算法在植保无人机路径规划中的研究进展

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农机化研究 2025年第3期47卷 1-9页

作者：张旭东于丽娅李少波张安思张保贵州大学机械工程学院贵阳550025 贵州大学省部共建公共大数据国家重点实验室贵阳550025

随着无人机系统技术、传感器技术和人工智能算法等相关技术的不断发展,植保无人机路径规划技术在农业生产中备受重视,并被广泛应用。作为航空植保的关键技术之一,植保无人机路径规划面临的是一个复杂且多约束的组合优化问题。传统算法... 详细信息

随着无人机系统技术、传感器技术和人工智能算法等相关技术的不断发展,植保无人机路径规划技术在农业生产中备受重视,并被广泛应用。作为航空植保的关键技术之一,植保无人机路径规划面临的是一个复杂且多约束的组合优化问题。传统算法往往难以得到理想的结果,而元启发式算法则因其高效率成为解决该类优化问题的有效手段。为此,首先介绍了农业航空中的路径规划,随后总结出了植保无人机路径规划的关键要素,并使用更为合理的分类方式进行归纳;其次,从算法层面对相关研究所采用的元启发式算法进行分类和梳理,并阐述了其在实际应用中的现状,且根据提出的分类方法和研究特点,全面地归纳了当前的研究成果;最后,针对目前植保无人机路径规划研究存在的问题提出了几条可行的发展思路。

关键词：农机航空植保无人机路径规划元启发式算法乡村振兴农业现代化

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A genome-wide association study on growth traits in orangespotted grouper(Epinephelus coioides) with RAD-seq genotyping

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Science China(Life Sciences) 2018年第8期61卷 934-946页

作者： Hui yu Xinxin You Jia Li Xinhui zhang Shuai zhang Shoujia Jiang Xueqiang Lin Hao-Ran Lin Zining Meng qiong Shi BGI Education Center University of Chinese Academy of Sciences Shenzhen Key Lab of Marine Genomics Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals BGI Academy of Marine SciencesBGI Marine BGI State Key Laboratory of Biocontrol Institute of Aquatic Economic Animals and the Guangdong Province Key Laboratory for Aquatic Economic Animals Sun Yat-Sen University Laboratory of Aquatic Genomics College of Life Sciences and Oceanography Shenzhen University

The orange-spotted grouper, Epinephelus coioides, is one of the most popular fish in China and Southeast Asian countries because of its important economic value. However, molecular mechanism underlying the growth of orange-spotted grouper has never been fully understood. Herein, we performed a genome-wide association study （GWAS） on a natural population of 198 individuals aiming to screen the whole genome of orange-spotted grouper for identification of growth-related loci by restriction-site associated DNA sequencing. In this research, 261,366 single nucleotide polymorphisms （SNPs） were developed, in which 110 SNPs were identified to be correlated with growth and 20 SNPs were further confirmed to be associated with both body weight and total length. From these identified SNPs, we annotated a total of 34 genes, including adgrb2, csnkzal, cers5, co122al, creb5, dndl, dzankl, dnail, npy2r, fat3, lrrk2, lrp5, map3k9, and so on. Among these candidate genes, npy2r （neuropeptide Y receptor Y2） was reported to play a critical role in growth of the orange-spotted grouper. In addition, population structure, principal component analysis, kinship matrix and linkage disequilibrium were examined to verify the accuracy and reliability of our GWAS results. Our data will also provide a valuable genetic resource for further marker-assisted selection program to improve growth quality in groupers.

关键词： orange-spotted grouper genome-wide association study (GWAS) restriction-site associated DNA sequencing (RAD-scq) growth traits npy2r

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