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Journal of Oceanology and Limnology 2023年第1期41卷 352-363页

作者： Fengjuan JIANG qingyao wang Jingjing DU Fu LÜ Qing NIE Weihong ZHAO College of Marine and Biological Engineering Yancheng Institute of TechnologyYancheng 224051China

The appropriate reference gene is a prerequisite for accurate normalization of gene expression level,and research on suitable reference genes in clam Cyclina sinensis is *** improve the situation,we selected five commonly used housekeeping genes,including β-actin,Elongation factor 1-α(EF1-α),Glyceraldehyde-3-pho sphate dehydrogenase(GAPDH),40S ribosomal protein S18(RPS18),and Tubulin a(TUB-α),then evaluated their expression stability in different adult tissues and under different experimental treatments(salinity stress and Vibrio parahaemolyticus infection).Their expression stability was analyzed by three frequently used programs,geNorm,NormFinder,and *** analysis indicated that multiple genes should be used for normalization,and we concluded that the reference gene combination GAPDH-RPS18-β-actin,should be used for qRT-PCR analysis in different tissues of *** under normal physiological *** the clams under salinity stress and Vibrio infection,EF1-α-GAPDHRPS18 was recommended as the gene combination for qRT-PCR ***-αwas generally poorly ranked by all programs,and should not be used in future *** study should provide fundamental support for accurate quantitative gene expression analysis of this species.

关键词： Cyclina sinensis reference gene different tissues salinity stress Vibrio infection

Characterization and prediction of tailpipe ammonia emissions from in-use China 5/6 light-duty gasoline vehicles

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Frontiers of Environmental Science & Engineering 2024年第1期18卷 71-81页

作者： Lewei Zeng Fengbin wang Shupei Xiao Xuan Zheng Xintong Li Qiyuan Xie Xiaoyang Yu Cheng Huang qingyao Hu Yan You Ye Wu College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060China State Key Laboratory of Engines Tianjin UniversityTianjin 300072China China Automotive Technology and Research Center Co.Ltd. Tianjin 300300China CATARC Automotive Test Center(Guangzhou)Co.Ltd. Guangzhou 511300China State Environmental Protection Key Laboratory of Cause and Prevention of Urban Air Pollution Complex Shanghai Academy of Environmental SciencesShanghai 200233China National Observation and Research Station of Coastal Ecological Environments in Macao Macao Environmental Research InstituteMacao University of Science and TechnologyMacao SAR 999078China School of Environment State Key Joint Laboratory of Environment Simulation and Pollution ControlTsinghua UniversityBeijing 100084China

On-road tailpipe ammonia (NH3) emissions contribute to urban secondary organic aerosol formation and have direct or indirect adverse impacts on the environment and human health. To understand the tailpipe NH3 emission characteristics, we performed comprehensive chassis dynamometer measurements of NH3 emission from two China 5 and two China 6 light-duty gasoline vehicles (LDGVs) equipped with three-way catalytic converters (TWCs). The results showed that the distance-based emission factors (EFs) were 12.72 ± 2.68 and 3.18 ± 1.37 mg/km for China 5 and China 6 LDGVs, respectively. Upgrades in emission standards were associated with a reduction in tailpipe NH3 emission. In addition, high NH3 EFs were observed during the engine warm-up period in cold-start cases owing to the intensive emissions of incomplete combustion products and suitable catalytic temperature in the TWCs. Notably, based on the instantaneous NH3 emission rate, distinct NH3–emitting events were detected under high/extra high velocity or rapid acceleration. Furthermore, NH3 emission rates correlated well with engine speed, vehicle specific power, and modified combustion efficiency, which were more easily accessible. These strong correlations were applied to reproduce NH3 emissions from China 5/6 LDGVs. The predicted NH3 EFs under different dynamometer and real-world cycles agreed well with existing measurement and prediction results, revealing that the NH3 EFs of LDGVs in urban routes were within 8.55–11.62 mg/km. The results presented here substantially contribute to improving the NH3 emission inventory for LDGVs and predicting on-road NH3 emissions in China.

关键词： NH3 instantaneous emissions Catalytic temperature Vehicle specific power Combustion efficiency Emission prediction

Rational construction of metal–base synergetic sites on Au/Mg-beta catalyst for selective aerobic oxidation of 5-hydroxymethylfurfural

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Journal of Energy Chemistry 2021年第11期30卷 599-609,I0014页

作者： Zhiguo Zhu Xiongjie Gao Xiuming wang Mengdie Yin qingyao wang Wanzhong Ren Bo wang Hongying Lü Weiping Liao College of Chemistry and Chemical Engineering Yantai University30 Qingquan RoadYantai 264005ShandongChina Library Yantai University30 Qingquan RoadYantai 264005ShandongChina Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology School of Chemistry and Chemical EngineeringLiaocheng UniversityLiaocheng 252059ShandongChina

The selective aerobic oxidation of biomass-derived 5-hydroxymethylfurfural to 2,5-furandicarboxylic acid (FDCA, a potential renewable substitution of fossil-based terephthalic acid to produce polyethylene 2,5-furandicarboxylate plastic) is an appealing transformation for constructing eco-friendly and sustainable chemical processes. Au supported catalysts have showed encouraging performances for this well-received conversion, whose catalytic behavior was greatly affected by the adopted support derived from the existence of metal-support interactions. Herein, a series of Mg-Beta zeolites were hydrothermally synthesized via developed structural reconstruction, which were employed as basic supports for Au catalysts to construct bifunctional catalysts. The relationship between structure (Au particle size, basicity within zeolites and Auδ+ contents) and FDCA yield was concretely established. The conclusion was made that the utilization of Mg-Beta zeolites with strong basicity as the support could not only improve the FDCA yield but also decrease the amount of additional base. Furthermore, the possible reaction mechanism was also proposed via tracking time-dependent variations of corresponding organics and controlled experiment. This work provides some guidance for rationally designing multifunctional catalysts in the view of integrating metal catalysts with metallosilicate zeolites, which was beneficial to the catalytic upgrading of organic compounds with multiple functional groups.

关键词： 5-Hydroxymethylfurfural Aerobic oxidation 2,5-Furandicarboxylic acid Bifunctional Au/Mg-Beta catalyst Structural reconstruction

Ti^(3+) self-doped TiO_2 photoelectrodes for photoelectrochemical water splitting and photoelectrocatalytic pollutant degradation

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Journal of Energy Chemistry 2016年第4期25卷 726-733页

作者： Chaoyi Wu Zhenggang Gao Shanmin Gao qingyao wang Hui Xu Zeyan wang Baibiao Huang Ying Dai College of Chemistry and Chemical Engineering Hunan University College of Chemistry and Materials Science Ludong University State Key Laboratory of Crystal Materials Shandong University

To improve the harvesting of visible light and reduce the recombination of photogenerated electrons and holes, Ti3+ self-doped TiO2 nanoparticles were synthesized and assembled into photoanodes with high visible light photoelectrochemical properties. X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectra, electron resonance spectroscopy and energy dispersive X-ray spectra were used to characterize the structure, crystallinity, morphology and other properties of the obtained nanoparticles. UV-visible diffuse reflectance spectra showed that the Ti3+ self-doped TiO2 nanoparticles had a strong absorption between 400 and 800 nm. Moreover, when hydrothermal treatment time was prolonged to 22 h, the heterogeneous junction was formed between the anatase and rutile TiO2, where the anatase particles exposed highly active {001} facets. Under visible light irradiation, the Ti3+ self-doped TiO2 electrode exhibited an excellent photoelectrocatalytic degradation of rhodamine B (RhB) and water splitting performance. Intriguingly, by selecting an appropriate hydrothermal time, the high photoconversion efficiency of 1.16% was achieved. (C) 2016 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.

关键词： Ti3+ self-doping Titanium Photoanode Water splitting Photoelectrocatalytic properties

Ultrasonic-assisted pyrolyzation fabrication of reduced SnO2-x/g-C3N4 heterojunctions： Enhance photoelectro- chemical and photocatalytic activity under visible LED light irradiation

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Nano Research 2016年第7期9卷 1969-1982页

作者： Kai Li Xiaoqiao Zeng Shanmin Gao Lu Ma qingyao wang Hui Xu Zeyan wang Baibiao Huang Ying Dai Jun Lu College of Chemistry and Materials Science Ludong University Yantai 264025 China Chemical Sciences and Engineering Division Argonne National Laboratory Argonne IL 60439 USA State Key Laboratory of Crystal Materials Shandong University Jinan 250100 China X-Ray Science Division Argonne National Laboratory Argonne IL 60439 USA

Novel SnO2-x/g-C3N4 heterojunction nanocomposites composed of reduced SnO2 nanoparticles and exfoliated g-CBN4 nanosheets were prepared by a convenient one-step pyrolysis method. The structural, morphological, and optical properties of the as-prepared nanocomposites were characterized in detail, indicating that the aggregation of g-C3N4 nanosheets was prevented by small, well-dispersed SnO2_x nanoparticles. The ultraviolet-visible spectroscopy absorption bands of the nanocomposites were shifted to a longer wavelength region than those exhibited by pure SnO2 or g-CgN4. The charge transfer and recombination processes occurring in the nanocomposites were investigated using linear scan voltammetry and electrochemical impedance spectroscopy. Under 30-W visible-light-emitting diode irradiation, the heterojunction containing 27.4 wt.% SnO2-x exhibited the highest photocurrent density of 0.0468 ***-2, which is 33.43 and 5.64 times larger than that of pure SnO2 and g-C3N4, respectively. The photocatalytic activity of the heterojunction material was investigated by degrading rhodamine B under irradiation from the same light source. Kinetic study revealed a promising degradation rate constant of 0.0226 min^-1 for the heterojunction containing 27.4 wt.% SnO2-x, which is 32.28 and 5.79 times higher than that of pure SnO2 and g-C3N4, respectively. The enhanced photoelectrochemical and photocatalytic performances of the nanocomposite may be due to its appropriate SnO2x content and the compact structure of the junction between the SnO2-x nanoparticles and the g-C3N4 nanosheets, which inhibits the recombination of photogenerated electrons and holes.

关键词： reduced SnO2x-x g-C3N4 heterojunctions photoelectrochemical light-emitting diodesource

Carbon materials from melamine sponges for supercapacitors and lithium battery electrode materials: A review

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Carbon Energy 2019年第2期1卷 253-275页

作者： Yanying Shi Guijing Liu Rencheng Jin Hui Xu qingyao wang Shanmin Gao School of Chemistry and Materials Science Ludong UniversityYantaiChina

With the increasing energy demand together with the deteriorating environment and decreasing fossil fuel resources,the development of highly efficient energy conversion and storage devices is one of the key challenges of both fundamental and applied research in energy *** sponges(MS)with low density,high nitrogen content,and high porosity have been used to design and obtain three‐dimensional porous carbon electrode *** importantly,they are inexpensive,environment‐friendly,and easy to *** have been many reports on the modification of carbonized MS and MS‐based composites for supercapacitor and lithium battery electrode *** this paper,recent studies on the fabrication of electrode materials using MS as raw materials have been mainly reviewed,including carbonation,doping activation,and composite modification of MS,and expectations for the development of porous carbon materials for energy storage as a reference with excellent performance,environment‐friendliness,and long life.

关键词： activation carbon materials composites electrode materials melamine sponges

Surveillance of enteric pathogens in imported seafood and environmental surfaces in five seafood markets before the outbreak of COVID-19

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Biosafety and Health 2021年第4期3卷 183-186页

作者： Songzhe Fu Wei wang qingyao wang Fenglan He Jingwei Hao Bo Pang College of Marine Science and Environment Dalian Ocean UniversityDalian 116023China Key Laboratory of Environment Controlled Aquaculture(KLECA) Ministry of EducationDalian 116023China The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control Nanchang Center for Disease Control and PreventionNanchang 330038China State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

To monitor the presence of enteric pathogens in imported seafood,a total of 140 seafood samples imported from eight overseas countries were collected from Beijing,Dalian,Shanghai,Guangzhou,and Wuhan seafood markets from June to November ***,116 viral,environmental swab samples were also collected from the Wuhan and Guangzhou seafood *** typical enteric bacterial pathogens(Aeromonas spp.,Shigella spp.,Salmonella spp.,Vibrio spp.,and Listeria monocytogenes)and four viruses(Rotavirus,Norovirus,Astrovirus,and Sapovirus)were detected *** showed that eight Vibrio parahaemolyticus isolates appeared in seafood imported to Dalian,Wuhan,Shanghai,Guangzhou,and *** contrast,Vibrio fluvialis and Aeromonas were isolated in another two *** was detected in one oyster sample imported from France and environmental surface in *** remaining pathogens were negative in all the samples being *** 120 *** isolates from the above countries,the genomic analysis revealed that sequence type ST1152 isolates imported from Canada were clustered with two *** isolates from *** study presented the first microbiological analysis of the Wuhan seafood market before the outbreak of COVID-19,which demonstrated that supervision should be strengthened to prevent enteric pathogens via imported seafood.

关键词： Vibrio parahaemolyticus Norovirus Wuhan seafood market Imported seafood

An Approximation Method of NURBS Curves in NC Machining

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武汉理工大学学报 2006年第S2期28卷 734-736页

作者： YUE Ying HAN qingyao wang Zhangqi (School of Mechanics Engineering,North China Electric Power University,Baoding 071003,China, School of Mechanics Engineering North China Electric Power UniversityBaoding 071003China

An algorithm for approximating arbitrary NURBS curve with straight line is ***,NURBS curve is acquired according to data points on the ***,Approximating arbitrary NURBS curve is based on *** resulting straight line approaches to the original curve with relatively fewer segments within the required *** example shows that the algorithm is simple and its approximation precision is *** method is most useful in numerical control to drive the cutter along straight line or circular paths.

关键词： NURBS curve dichotomy line approximation

以富CO_(2)吸收液为汲取液的沼液中水正渗透回收特性研究

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农业机械学报 2024年第6期55卷 317-324页

作者：汪洋石明菲贺清尧晏水平华中农业大学工学院武汉430070 农业农村部长江中下游农业装备重点实验室武汉430070

针对沼气工程存在的沼液量大难处理问题及沼气提纯的需求,提出将正渗透技术与沼气CO_(2)化学吸收分离耦合,探究了沼气CO_(2)化学吸收中的富CO_(2)吸收液作为正渗透汲取液从沼液中回收水及浓缩沼液的可行性,并以沼液浓缩过程中的水通量... 详细信息

针对沼气工程存在的沼液量大难处理问题及沼气提纯的需求,提出将正渗透技术与沼气CO_(2)化学吸收分离耦合,探究了沼气CO_(2)化学吸收中的富CO_(2)吸收液作为正渗透汲取液从沼液中回收水及浓缩沼液的可行性,并以沼液浓缩过程中的水通量、沼液浓缩倍数、沼液氨氮截留率与吸收剂反向传质通量为指标,考察了汲取液种类、汲取液浓度与其他操作参数对正渗透水回收性能的影响。结果表明,富CO_(2)吸收液作为汲取液从沼液中回收水并浓缩沼液具有可行性,且随着汲取液浓度、流量和温度的增加,沼液中水向汲取液的传质通量增加,沼液浓缩倍数也相应增加,但沼液中氨氮截留率下降,同时汲取液中的吸收剂溶质向沼液的反向传质通量也增加。当采用浓度2.5mol/L、CO_(2)负荷0.5mol/mol的富CO_(2)甘氨酸钾溶液作为汲取液,汲取液温度为70℃、流速为150mL/min、沼液室温及流速为150mL/min时,采用正渗透技术从沼液中回收水的初始通量达8.05L/(m^(2)·h),经过4h运行后,沼液浓缩倍数为1.18,氨氮截留率为84.13%,反向吸收剂通量仅为2.94g/(m^(2)·h)。

关键词：正渗透沼液浓缩水回收 CO_(2)捕获膜分离

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人工智能在老年慢性病共存病人自我管理中的应用进展

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护理研究 2022年第5期36卷 869-873页

作者：王文王向佳谢江汪倾遥文静丛丽湖南师范大学医学院湖南410013

对老年慢性病共存病人自我管理现状、人工智能对老年慢性病共存病人自我管理的影响、人工智能产品应用情况及使用影响因素进行综述,旨在为我国老年慢性病共存病人智慧化自我管理提供借鉴和参考。

关键词：老年人慢性病人工智能自我管理康复护理影响因素综述