Identification of suitable reference genes for quantitative gene expression analysis in clam Cyclina sinensis under salinity stress and Vibrio infection

作     者：Fengjuan JIANG Qingyao WANG Jingjing DU Fu LÜ Qing NIE Weihong ZHAO Fengjuan JIANG;Qingyao WANG;Jingjing DU;Fu LU;Qing NIE;Weihong ZHAO

作者机构：College of Marine and Biological EngineeringYancheng Institute of TechnologyYancheng 224051China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2023年第41卷第1期

页      面：352-363页

核心收录：

学科分类：0710[理学-生物学] 0707[理学-海洋科学] 0906[农学-兽医学] 09[农学] 

基　　金：Supported by the funding for school-level research projects of Yancheng Institute of Technology(No.xjr2019047) the National Natural Science Foundation of China(No.31902362) 

主　　题：Cyclina sinensis reference gene different tissues salinity stress Vibrio infection 

摘      要：The appropriate reference gene is a prerequisite for accurate normalization of gene expression level,and research on suitable reference genes in clam Cyclina sinensis is *** improve the situation,we selected five commonly used housekeeping genes,including β-actin,Elongation factor 1-α(EF1-α),Glyceraldehyde-3-pho sphate dehydrogenase(GAPDH),40S ribosomal protein S18(RPS18),and Tubulin a(TUB-α),then evaluated their expression stability in different adult tissues and under different experimental treatments(salinity stress and Vibrio parahaemolyticus infection).Their expression stability was analyzed by three frequently used programs,geNorm,NormFinder,and *** analysis indicated that multiple genes should be used for normalization,and we concluded that the reference gene combination GAPDH-RPS18-β-actin,should be used for qRT-PCR analysis in different tissues of *** under normal physiological *** the clams under salinity stress and Vibrio infection,EF1-α-GAPDHRPS18 was recommended as the gene combination for qRT-PCR ***-αwas generally poorly ranked by all programs,and should not be used in future *** study should provide fundamental support for accurate quantitative gene expression analysis of this species.