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Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae

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Microsystems & Nanoengineering 2024年第1期10卷 89-103页

作者： Zhongyi Guo Nana Ai Wei Ge qingsong xu Department of Electromechanical Engineering Faculty of Science and TechnologyUniversity of MacaoMacaoChina Department of Biomedical Sciences and Centre of Reproduction Development and Aging(CRDA)Faculty of Health SciencesUniversity of MacaoMacaoChina

This paper presents the design of a vision-based automated robotic microinjection system for batch injection of both zebrafish embryos and larvae.A novel visual recognition algorithm based on an automatic threshold and excessive dilatation is introduced to accurately identify the center of zebrafish embryos and larval yolks.A corresponding software system is developed using the producer-consumer model as the framework structure,and a friendly user interface is designed to allow operators to choose from a range of desired functions according to their different *** addition,a novel microstructural agarose device is designed and fabricated to simultaneously immobilize mixed batches of embryos and ***,a prototype microinjection system is fabricated by integrating hardware devices with visual *** experimental study is conducted to verify the performance of the robotic microinjection *** results show that the reported system can accurately identify zebrafish embryos and larvae and efficiently complete batch microinjection tasks of the mixtures with an injection success rate of 92.05%in 13.88 s per *** with manual and existing microinjection systems,the proposed system demonstrates the merits of versatility,excellent efficiency,high success rate,high survival rate,and sufficient stability.

关键词： zebra automated hardware

Deep learning for cross-region streamflow and flood forecasting at a global scale

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The Innovation 2024年第3期5卷 99-109页

作者： Binlan Zhang Chaojun Ouyang Peng Cui qingsong xu Dongpo Wang Fei Zhang Zhong Li Linfeng Fan Marco Lovati Yanling Liu Qianqian Zhang State Key Laboratory of Mountain Hazards and Engineering Resilience Institute of Mountain Hazards and EnvironmentChinese Academy of SciencesChengdu 610299China State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of TechnologyChengdu 610059China Data Science in Earth Observation Technical University of Munich80333 MunichGermany State Key Laboratory of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of SciencesXi’an 710061China Department of Civil Engineering McMaster UniversityHamiltonON L8S 4L8Canada Chengdu University of Information Technology Chengdu 610225China

Streamflow and flood forecasting remains one of the long-standing challenges in *** physically based models are hampered by sparse parameters and complex calibration procedures particularly in ungauged *** propose a novel hybrid deep learning model termed encoder-decoder double-layer long short-term memory(ED-DLSTM)to address streamflow forecasting at global scale for all(gauged and ungauged)*** historical datasets,ED-DLSTM yields a mean Nash-Sutcliffe efficiency coefficient(NSE)of 0.75 across more than 2,000 catchments from the United States,Canada,Central Europe,and the United Kingdom,highlighting improvements by the state-of-the-art machine learning over traditional hydrologic ***,ED-DLSTM is applied to 160 ungauged catchments in Chile and 76.9%of catchments obtain NSE>0 in the best *** interpretability of cross-region capacities of ED-DLSTM are established through the cell state induced by adding a spatial attribute encoding module,which can spontaneously form hydrological regionalization effects after performing spatial coding for different *** study demonstrates the potential of deep leaning methods to overcome the ubiquitous lack of hydrologic information and deficiencies in physical model structure and parameterization.

关键词： forecasting flood flow

新时代下军队院校《卫生法学》课程教学改革路径探究

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中国卫生法制 2024年第3期32卷 71-75,109页

作者：徐青松海军军医大学基础医学院上海200433

“为谁培养人、培养什么人、怎样培养人”始终是教育的根本问题。2023年9月,习近平主席在致国防科技大学建校70周年的贺信中指出,要“坚持立德树人、为战育人”。进入新时代,军队院校的《卫生法学》课程教学改革应紧紧围绕这个目标所向... 详细信息

“为谁培养人、培养什么人、怎样培养人”始终是教育的根本问题。2023年9月,习近平主席在致国防科技大学建校70周年的贺信中指出,要“坚持立德树人、为战育人”。进入新时代,军队院校的《卫生法学》课程教学改革应紧紧围绕这个目标所向,剖析当前军队院校《卫生法学》课程教学改革中存在的“短板”,尝试“互联网+”与沉浸式情景模拟教学方法在《卫生法学》课程中的运用,探索“立德树人、为战育人”理念下的军队院校《卫生法学》课程教学改革新路径。

关键词：卫生法学新医改教学改革务实导向

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宁夏清分结算中心同城灾备系统建设方案研究

现代计算机 2024年第14期30卷 100-106页

作者：江海琦徐青松宁夏路网监测与应急处置中心银川750011 中远海运科技股份有限公司上海200135

随着省界收费站取消,全国高速公路收费“一张网”运行,承担数据汇聚、计费管理、清分结算等重要功能的省级清分结算中心系统是全省联网收费的大脑,也是全国“一张网”的核心。宁夏清分结算中心在需求分析基础上,结合灾备技术架构模型,... 详细信息

随着省界收费站取消,全国高速公路收费“一张网”运行,承担数据汇聚、计费管理、清分结算等重要功能的省级清分结算中心系统是全省联网收费的大脑,也是全国“一张网”的核心。宁夏清分结算中心在需求分析基础上,结合灾备技术架构模型,进行同城灾备系统建设方案研究,以实现省级清分结算中心系统的高可靠性、高可用性和7×24小时不间断服务。

关键词：省中心同城灾备中心信创两地三中心

Chemometrics and modernization of traditional Chinese medicine

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Science China Chemistry 2008年第8期51卷 718-728页

作者： LIANG YiZeng YI LunZhao xu qingsong Research Center of Modernization of Traditional Chinese Medicine Central South UniversityChangsha 410083China College of Mathematics Central South UniversityChangsha 410083China

Development of chromatographic fingerprinting and its related chemometric methods in the research of quality control of traditional Chinese medicines(TCMs) are discussed. The quality control methods for guarantying the authentication and stability of products and semi-products of TCMs are firstly assessed. The technique based on chromatographic fingerprinting is essentially a kind of high-through put and integral tools to explore the complexity of herbal medicines. In order to further control the comprehensive quality of TCMs,confirmation and identification of their important chemical components are necessary. Some new strategies are proposed to trace the chemical changes of chromatographic fingerprints both in product processing and/or after their administration by modern chromatographic techniques and chemometrics. Combined with systems biology and bioinformatics,it seems possible for one to reveal the working mechanism of TCMs and to further control their intrinsic quality comprehensively.

关键词： modernization of traditional Chinese medicine chemometrics chromatographic fingerprinting quality control

Irradiation Resistance of CoCrCuFeNi High Entropy Alloy under Successive Bombardment

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金属学报:英文版 2023年第9期36卷 1482-1492页

作者： Rui Li Lei Guo Yu Liu qingsong xu Qing Peng School of Mechanical Engineering University of Science and Technology BeijingBeijing100083China Physics Department King Fahd University of Petroleum and Minerals31261DhahranSaudi Arabia K.A.CARE Energy Research and Innovation Center at Dhahran 31261DhahranSaudi Arabia Interdisciplinary Research Center for Hydrogen and Energy Storage King Fahd University of Petroleum and Minerals31261DhahranSaudi Arabia

With the excellent irradiation resistance due to the chemical complexity,high entropy alloys have attracted considerable attention in the design of nuclear structural ***,their performance under successive bombardments remains *** this study,we have investigated the irradiation resistance of equiatomic CoCrCuFeNi HEA compared with Ni metal using molecular dynamics *** evolution of defects such as point defects,defect clusters and dislocations after 400 times of bombardments is *** results show that CoCrCuFeNi has fewer point defects than Ni ***,CoCrCuFeNi has smaller interstitial clusters size,lower interstitial-type Frank partial dislocation density,shorter average interstitial-type Frank partial dislocation length and higher average vacancy formation *** findings suggest that CoCrCuFeNi has superior radiation resistance under successive bombardments due to its high entropy effect.

关键词： High entropy alloys Irradiation resistance Defects Dislocations

Research on an Agile Manufacturing Information System

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Journal of Systems Engineering and Electronics 2000年第3期11卷 22-27页

作者： xu, qingsong Fan, Yushun Tao, Dan Dept. of Automation Tsinghua University Beijing 100084 P. R. China

With the more intense competition in the worldwide market, the agile virtual enterprise (AVE) will become a new organization form of manufacturing enterprise. To support the new relationship, an agile manufacturing information system (AMIS) is described in this paper which serves to assist the virtual enterprise to manage and control information flow among collaborating partners. Based on the requirement analysis of virtual information system, the architecture, functions, features and execution scheme of AMIS are discussed in detail.

关键词： Agile manufacturing information system (AMIS) Dynamic collaboration Distributed workflow management Agent.

Fabrication of Flexible Transparent Conductive Films with Silver Nanowire by Vacuum Filtration and PET Mold Transfer

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Journal of Materials Science & Technology 2016年第2期32卷 158-161页

作者： Wei xu qingsong xu Qijin Huang Ruiqin Tan Wenfeng Shen Weijie Song Ningbo Institute of Material Technology and Engineering Chinese Academy of SciencesNingbo 315201China Ningbo Key Laboratory of Silicon and Organic Thin Film Optoelectronic Technologies Ningbo 315201China Faculty of information Science and Engineering Ningbo UniversityNingbo 315211China

The flexible transparent conductive films （FrCFs） of silver nanowire-polyethylene terephthalate （AgNW- PET） were prepared by a facile method including vacuum filtration and mold transferring. The effect of silver nanowire weight density on the optical and electrical properties of films, as well as the electrical percolation was investigated. The obtained typical AgNW-PET film exhibited high figure of merit of 31.3 × 10^-3 Ω^-1 with low sheet resistance of 4.95 D sq^-1 and high transparency at 550 nm of 83.0% （excluding PET substrate）. The resulting FTCFs based on PET substrate with high transmittance and low sheet resistance have a great potential in the application of high-performance flexible electronics and photovoltaic devices.

关键词： Silver nanowires Nanoparticles Percolation threshold Transparent conductive film

Multiple-Cell Recognition and Path Planning for Robotic Microinjection System

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Multiple-Cell Recognition and Path Planning for Robotic Micr...