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OGG1 inhibition suppresses African swine fever virus replication

Virologica Sinica 2023年第1期38卷 96-107页

作者： Jie Fan Xinqian Lv Saixia Yang Shuxian Geng Jifei Yang Yaru Zhao Zhonghui Zhang Zhijie Liu Guiquan Guan Jianxun Luo Qiaoying Zeng Hong Yin qingli niu College of Veterinary Medicine Gansu Agricultural UniversityLanzhou730070China African Swine Fever Regional Laboratory of China(Lanzhou) State Key Laboratory of Veterinary Etiological BiologyKey Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis Yangzhou UniversityYangzhou225009China

African swine fever virus(ASFV)is an important pathogen that causes a highly contagious and lethal disease in swine,for which neither a vaccine nor treatment is *** DNA repair enzyme 8-oxoguanine DNA glycosylase 1(OGG1),which excises the oxidative base lesion 8-oxo-7,8-dihydroguanine(8-oxoG),has been linked to the pathogenesis of different diseases associated with viral ***,the role of OGG1-base excision repair(BER)in ASFV infection has been poorly *** study aimed to characterize the alteration of host reactive oxygen species(ROS)and OGG1 and to analyse the role of OGG1 in ASFV *** found that ASFV infection induced high levels and dynamic changes in ROS and 8-oxoG and consistently increased the expression of *** yield,transcription level,and protein synthesis were reduced in ASFV-infected primary alveolar macrophages(PAMs)treated by TH5487 or SU0268 inhibiting *** expression of BER pathway associated proteins of ASFV was also suppressed in OGG1-inhibited ***,OGG1 was found to negatively regulate interferonβ(IFN-β)production during ASFV infection and IFN-βcould be activated by OGG1 inhibition with TH5487 and SU0268,which blocked OGG1 binding to ***,the interaction of OGG1 with viral MGF360-14-L protein could disturb IFN-βproduction to further affect ASFV *** results suggest that OGG1 plays the crucial role in successful viral infection and OGG1 inhibitors SU0268 or TH5487 could be used as antiviral agents for ASFV infection.

关键词： African swine fever virus(ASFV) 8-oxoguanine DNA glycosylase 1(OGG1) 8-oxo-7,8-dihydroguanine(8-oxoG) Inhibitors Antiviral effect

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Identification and molecular survey of Borrelia burgdorferi in sika deer from Jilin Province in China

Identification and molecular survey of Borrelia burgdorferi ...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Bintao Zhai qingli niu Jifei Yang Zhijie Liu Hong Yin Qiaoying Zeng The College of Veterinary Medicine Gansu Agricultural University State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

Background: Lyme disease is caused by Borrelia burgdorferi. Various animals and wildlife are natural reservoirs of the disease. It is reported that sika deer may as an important reservoir hosts for several species of Borrelia burgdorferi and transmit them to domestic humans and animals. However, little is known about the presence of Borrelia pathogens in sika deer in China. The prevalence of Borrelia spp. in sika deer from Jilin Province in China was assessed. Methods: Seventy-one blood samples of sika deer were collected at four sites from Jilin Province during June and July, 2015, and tested by nested PCR based on the 5s-23 s rRNA, Osp A, Fla and 16 S rRNA genes of the Borrelia burgdorferi. The PCR results were confirmed by DNA sequencing based on long fragment of 16 S rRNA gene. The identification of Borrelia burdorferi species were determined according to obtained gene sequences of selected positive samples from each sampling area. Results: 12 samples were positive for Borrelia spp.(16.9%, 95% CI=10.5-28.8). After sequences alignment, three representative positive sequences from three sites were identified as being infected with Borrelia spp. that have high similarities(96.7-99.6%) with B. garinii species of the strain T-MDJ(HM007279) from China. Conclusion: This is the first report of Borrelia garinii in sike deer from Jilin Province, in China. The data obtained indicates that sika deer might be a nature host and a potential source spreads this pathogen to domestic animals, even human in China.

关键词： Borrelia burgdorferi sika deer identification survey

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Molecular Survey and Genetic Identification of Anaplasma phagocytophilum of horses in Zhaosu County of Xinjiang Province, China

Molecular Survey and Genetic Identification of Anaplasma pha...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Zhenguo Wang qingli niu Jifei Yang Jianxun Luo Hong Yin Zhijie Liu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Science

Human granulocytic anaplasmosis(HGA) is a tick transmitted disease caused by the bacterium Anaplasma phagocytophilium. The presence of the pathogen in various of natural hosts including sheep, goats, cattle as well as ticks has been detected by nucleotide acid-based molecular methods, but not reported in horse in China so far. In this study, we detected for Anaplasma phagocytophilum in 100 horse blood DNA samples from Zhaosu County of Xinjiang Province. A 16 S rRNA based species-specific PCR method was used and a target of 641 bp fragment was amplified. The results showed that the positive rate was 6%.Sequence analysis of the 16 S rRNA gene revealed that only one genotype of A. phagocytophilum presents in the horses. These findings should have raised the public health concerns in the investigated region.

关键词： Anaplasma phagocytophilum 16S rRNA gene Horse Tick-borne disease

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In vitro infection of bovine and ovine red blood cells with Theileria annulata schizonts

In vitro infection of bovine and ovine red blood cells with ...

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中国奶业协会第26次繁殖学术年会暨国家肉牛牦牛/奶牛产...

作者： Youquan Li Guiquan Guan Zhijie Liu Jifei Yang qingli niu Jianxun Luo Hong Yin State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Science

Theileria annulata,the causative agents of tropical theileriosis,is protozoan parasites that cause lymphoproliferative diseases of *** vivo,parasitized cells undergo clonal expansion and in?Itrate both lymphoid and non-lymphoid tissues of the infected *** this study,in vitro infection of bovine and ovine red blood cells with Theileria annulata schizonts is firstly carried *** experiments showed that Theileria annulata schizonts can infect bovine red blood cells from cattle 2 and cattle 30 in vitro,and also infect ovine red blood cells from sheep 2 and sheep 82 in ***,*** schizonts can infect both bovine red blood cells and ovine red blood cells in *** proliferation of Theileria annulata schizonts-infected lymphocytes is obviously suppressed by bovine red blood cells and ovine red blood *** schizonts could provide an intriguing model for the study of parasite-host interactions in vitro in the future.

关键词： In vitro infection bovine red blood cells ovine red blood cells Theileria annulata schizonts

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计算机技术在图像轮廓提取中的有效应用

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现代电子技术 2016年第10期39卷 34-36页

作者：于海燕牛庆丽郑州科技学院信息工程学院河南郑州450064

分析了图像轮廓提取中计算机技术的有效应用。针对传统图像边缘检测中存在的问题在VC的环境下进行研究,结合计算机技术,采用灰度阈值法进行图像分割,使用数学形态学方法修补二值图像缺陷,优化设计轮廓提取方法,以便提升图像处理效果。... 详细信息

分析了图像轮廓提取中计算机技术的有效应用。针对传统图像边缘检测中存在的问题在VC的环境下进行研究,结合计算机技术,采用灰度阈值法进行图像分割,使用数学形态学方法修补二值图像缺陷,优化设计轮廓提取方法,以便提升图像处理效果。经过研究发现,将计算机技术应用到图像轮廓提取中,精度高达98.0%,能满足工程测量的实际需要。在图像轮廓提取中,应用计算机技术进行图像轮廓提取,有助于达到最佳的图像处理效果,提高图像价值,发挥积极应用效果。

关键词：边缘检测计算机技术灰度阈值图像轮廓提取

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基于四聚体结构实现对光开关的控制研究

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传感器与微系统 2023年第3期42卷 40-44页

作者：牛庆丽朱耀琴郑州科技学院信息工程学院河南郑州450064 南京理工大学计算机科学与工程学院江苏南京210014

提出了一种由4个纳米棒组成的类似矩形的四聚体结构,这种结构可通过偏振角的变化实现对光开关的控制。利用有限元法对偏振角为0°和90°时的透射谱、电场及电荷分布进行了分析与研究。研究表明,设计的这种四聚体结构的透射光谱对偏振角... 详细信息

提出了一种由4个纳米棒组成的类似矩形的四聚体结构,这种结构可通过偏振角的变化实现对光开关的控制。利用有限元法对偏振角为0°和90°时的透射谱、电场及电荷分布进行了分析与研究。研究表明,设计的这种四聚体结构的透射光谱对偏振角的变化相当敏感,一旦偏振角发生90°的变化即可实现该结构对光开关的控制,且开关比可高达27.81 dB。该结构的开关效应主要是不同的偏振态导致横竖纳米棒具有不同的耦合强度。然后,又对不同的结构周期和参数对透射特性的影响进行了研究。研究表明,光开关的响应特性均可通过改变结构单元周期、d_(1)、l_(1)、l_(2)完成调节。研究结果为制造高性能的纳米光开关提供了非常重要的理论依据。

关键词：光开关偏振角有限元分析亚波长结构

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Simultaneous detection of piroplasms infections in field ticks by Reverse Line Blotting(RLB)

Simultaneous detection of piroplasms infections in field tic...

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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

作者： Mirza Omar Abdallah qingli niu Guiquan Guan Jifei Yang Ze Chen Jianxun Luo Hong Yin State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Science Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Piroplasmosis is caused by tick-borne haemoprotozoan parasites in the genera of Theileria and *** disease leads to clinical infections in domestic and wild animals causing economic *** of those intracellular apicomplexan parasites are highly pathogenic for sheep/goat,cattle and horse with high mortality and *** reports show the growing risks associated with ticks and tick-borne diseases worldwide due to expanding tick populations,global warming,and increased contacts between human,animals,and *** majority of tick-borne pathogen species have been reported in the vertebrate hosts they infect but less in the *** addition,several tick-borne pathogens with known tick vectors display a broader host range than formerly *** of pathogens in ticks allowed us to distinguish pathogens that infected the ticks before they attached to the hosts from the pathogens sucked by ticks during their blood *** this study,the RLB assay is used to investigate the presence of piroplasms in ticks collected from three provinces in ***-Reverse line blot(RLB) analysis couples a universal PCR amplification with the specific hybridization with multiple probes,thus increasing sensitivity and allowing for the simultaneous detection of multiple *** specific probes of 16 piroplasms were designed on the basis of hypervariable region 4(V4 region) of 18 S rRNA *** PCR was first carried out to amplify the V4 region of 18 S gene using a pair of universal primers specific for all members in the genera of Theileria and Babesia;the 18 S rRNA gene fragment was then cloned and sequenced;the positive plasmids were identified by PCR and purified and then used for sensitive *** Babesia and seven Theileria can be detected simultaneously on this *** sensitivity of RLB for each parasite detection is 10%(Babesia sp Hebei),10%(Babesia sp Ningxian),10%(Babesia sp Lintan) 10%(Babesia sp Tianzhu),10%(Babesia sp Xinjiang),10%

关键词： Piroplasms Ticks Reverse line blot(RLB) Detection

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Molecular evidence of Rickettsia massiliae and Anaplasma bovis in Hyalomma anatolicum anatolicum ticks from Xinjiang Uygur Autonomous Region, northwest China

Molecular evidence of Rickettsia massiliae and Anaplasma bov...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Peifa Yu Zhijie Liu qingli niu Jifei Yang Mirza Omar Abdallah Ze Chen Guangyuan Liu Jianxun Luo Hong Yin State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Science

Rickettsioses and anaplasmoses are a focus of increasing medical interest worldwide. To date, although the agents of these diseases have been reported in ticks in China, the presence of spotted fever group rickettsiae(SFGR), Anaplasma phagocytophilum and Anaplasma bovis in Hyalomma anatolicum anatolicum were little known. In this study, we investigated the occurrence of these pathogens in Hy. a. anatolicum from Shache County, Xinjiang Uygur Autonomous Region(XUAR), China, and performed phylogenetic analysis based on the omp A and 16 S rRNA genes, respectively. Our results indicated one tick specimen sample was positive for SFGR, two for A. bovis and three for A. phagocytophilum by PCR. Sequences and phylogenetic analyses showed that the SFGR agent detected was closely related to Rickettsia massiliae. However, one isolate PCR-positive for A. bovis was affiliated to the group of A. bovis, with another isolate clustered with the group of A. platys, and three isolates PCR-positive for A. phagocytophilum were closely related to Ehrlichia sp. To our knowledge, this is the first report of R. massiliae, A. bovis and A. platys-like pathogen presence in Hy. a. anatolicum, and provides evidence for the existence of these pathogens in Shache County, XUAR, China.

关键词： Rickettsia massiliae Anaplasma bovis Hyalomma anatolicum anatolicum Xinjiang Uygur Autonomous Region(XUAR)

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应用型普通本科院校管理信息系统课程改革与探索

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中国教育技术装备 2012年第21期 58-59页

作者：李志伟牛庆丽郑州科技学院信息工程学院郑州450064

在应用型普通本科院校管理信息系统教学中,由于课程的性质,若采用传统的教学方法进行授课,学生对该课程的学习兴趣不大。通过分析以及在教学过程中的实践,提出改革措施,以加强授课过程中的交互,增强教学效果。

关键词：管理信息系统课程改革案例驱动

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宿主蛋白RPSA在非洲猪瘟病毒复制过程中的作用研究

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中国兽医科学 2024年第9期54卷 1149-1156页

作者：邵程祥张忠辉孙华林赵亚茹杨吉飞关贵全殷宏武军元牛庆丽塔里木大学动物科学与技术学院新疆阿拉尔843300 中国农业科学院兰州兽医研究所兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室国家非洲猪瘟区域实验室甘肃兰州730046 江苏高校动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009

本研究旨在探究宿主RPSA蛋白对非洲猪瘟病毒(African swine fever virus,ASFV)复制的影响,以及病毒蛋白B602L与RPSA之间的相互作用。利用RPSA重组质粒pcDNA3.1-Flag-RPSA转染293T细胞,通过免疫荧光检测B602L和RPSA蛋白的亚细胞定位,通... 详细信息

本研究旨在探究宿主RPSA蛋白对非洲猪瘟病毒(African swine fever virus,ASFV)复制的影响,以及病毒蛋白B602L与RPSA之间的相互作用。利用RPSA重组质粒pcDNA3.1-Flag-RPSA转染293T细胞,通过免疫荧光检测B602L和RPSA蛋白的亚细胞定位,通过免疫共沉淀(Co-IP)验证它们之间的相互关系。通过荧光定量PCR和Western-blot分析ASFV感染IPAM细胞在不同时间点RPSA的表达差异。此外,通过在IPAM细胞中过表达或干扰RPSA的表达,分析RPSA对ASFV复制的影响。研究结果表明,ASFV感染IPAM细胞后,RPSA蛋白的表达随时间延长而逐渐增加,表明RPSA蛋白可能在病毒感染中发挥作用。通过过表达RPSA可抑制病毒复制,而干扰RPSA则导致病毒复制增强,进一步表明RPSA在ASFV复制过程中发挥重要作用。最后,Co-IP和亚细胞定位结果证明病毒蛋白B602L与宿主蛋白RPSA之间存在相互作用。综上所述,本研究揭示了宿主蛋白RPSA在ASFV复制过程中的作用,为进一步研究病毒与宿主之间的相互作用机制奠定了基础,也有助于深入了解ASFV的感染机制为疾病控制提供新的线索。

关键词：非洲猪瘟病毒 B602L RPSA 相互作用

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