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Sympathetic Nerve Mediated Spinal Glia Activation Underlies Itch in a Cutaneous T-Cell Lymphoma Model

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Neuroscience Bulletin 2022年第4期38卷 435-439页

作者： Fang Tong Qianru He Wan-Jie Du Huan Yang Dongping Du Shaofeng Pu Qingjian Han State Key Laboratory of Medical Neurobiology and MOE Frontier Center for Brain Science Institutes of Brain ScienceFudan UniversityShanghai 200433China Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-Innovation Center of NeuroregenerationJiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury RepairNantong UniversityNantong 226001China Pain Management Center Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200233China

Dear Editor,Cancer-associated itch can be described as pruritus associated with malignancy.Although intractable itch associated with specific types of cancer afflicts patients badly,few effective treatments are available due to limited knowledge about the mechanisms of such itch.Globally,cancer-associated itch is an uncommon symptomin malignancy.

关键词： malignancy cancer Cancer

Mechanosensitive Ion Channel TMEM63A Gangs Up with Local Macrophages to Modulate Chronic Post-amputation Pain

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Neuroscience Bulletin 2023年第2期39卷 177-193页

作者： Shaofeng Pu Yiyang Wu Fang Tong Wan-Jie Du Shuai Liu Huan Yang Chen Zhang Bin Zhou Ziyue Chen Xiaomeng Zhou Qingjian Han Dongping Du Pain Management Center Shanghai Jiao Tong University Afliated Sixth People’s HospitalShanghai 200233China State Key Laboratory of Medical Neurobiology and MOE Frontier Center for Brain Science Institutes of Brain ScienceFudan UniversityShanghai 200032China

Post-amputation pain causes great sufering to amputees,but still no efective drugs are available due to its elusive mechanisms.Our previous clinical studies found that surgical removal or radiofrequency treatment of the neuroma at the axotomized nerve stump efectively relieves the phantom pain aficting patients after amputation.This indicated an essential role of the residual nerve stump in the formation of chronic post-amputation pain(CPAP).However,the molecular mechanism by which the residual nerve stump or neuroma is involved and regulates CPAP is still a mystery.In this study,we found that nociceptors expressed the mechanosensitive ion channel TMEM63A and macrophages infltrated into the dorsal root ganglion(DRG)neurons worked synergistically to promote CPAP.Histology and qRT-PCR showed that TMEM63A was mainly expressed in mechanical pain-producing non-peptidergic nociceptors in the DRG,and the expression of TMEM63A increased signifcantly both in the neuroma from amputated patients and the DRG in a mouse model of tibial nerve transfer(TNT).Behavioral tests showed that the mechanical,heat,and cold sensitivity were not afected in the Tmem63a-/-mice in the naïve state,suggesting the basal pain was not afected.In the infammatory and post-amputation state,the mechanical allodynia but not the heat hyperalgesia or cold allodynia was signifcantly decreased in Tmem63a-/-mice.Further study showed that there was severe neuronal injury and macrophage infltration in the DRG,tibial nerve,residual stump,and the neuromalike structure of the TNT mouse model,Consistent with this,expression of the pro-infammatory cytokines TNFα,IL-6,and IL-1βall increased dramatically in the DRG.Interestingly,the deletion of Tmem63a signifcantly reduced the macrophage infltration in the DRG but not in the tibial nerve stump.Furthermore,the ablation of macrophages signifcantly reduced both the expression of Tmem63a and the mechanical allodynia in the TNT mouse model,indicating an interaction between nociceptors and macrophages,and that these two

关键词： Mechanosensitive ion channel TMEM63A Post-amputation pain Tibial nerve transfer Macrophage

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一种基于RGB-D的人体关节点定位方法

郑州大学学报（工学版） 2018年第5期39卷 33-38页

作者：张青建韩建平杭州电子科技大学计算机学院浙江杭州310016

提出了一种基于RGB-D的人体关节点定位方法:对kinect获取的深度图像滤波膨胀细化处理得到人体骨架线,根据角点检测算法得到人体骨架线的端点及折点,然后利用运动目标的跟踪算法对彩色图像中的人脸部跟踪定位,最后结合人体各刚架比例关系... 详细信息

提出了一种基于RGB-D的人体关节点定位方法:对kinect获取的深度图像滤波膨胀细化处理得到人体骨架线,根据角点检测算法得到人体骨架线的端点及折点,然后利用运动目标的跟踪算法对彩色图像中的人脸部跟踪定位,最后结合人体各刚架比例关系,定位出人体的15个关节点.试验结果表明,与直接根据人体各刚件比例关系定位关节点的方法相比,此方法的关节点平均定位准确率提高了7%,其中头节点定位准确率提高了42%.

关键词： RGB-D kinect 图像细化角点检测脸部跟踪关节点定位

Effectiveness of an ocular adhesive polyhedral oligomeric silsesquioxane hybrid thermo-responsive FK506 hydrogel in a murine model of dry eye

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Bioactive Materials 2022年第3期7卷 77-91页

作者： Yi han Lu Jiang Huihui Shi Chenfang Xu Minting Liu qingjian Li Lan Zheng Hong Chi Mingyue Wang Zuguo Liu Mingliang You Xian Jun Loh Yun-Long Wu Zibiao Li Cheng Li Fujian Provincial Key Laboratory of Ophthalmology and Visual Science&Ocular Surface and Corneal Diseases Eye Institute&Affiliated Xiamen Eye CenterSchool of MedicineXiamen UniversityXiamen361102China Institute of Materials Research and Engineering A*STAR(Agency for ScienceTechnology and Research)Singapore138634Singapore School of Chemical Sciences University of Chinese Academy of ScienceBeijing100049China Key Laboratory of Marine Materials and Related Technologies Zhejiang Key Laboratory of Marine Materials and Protective TechnologiesNingbo Institute of Material Technology and EngineeringChinese Academy of ScienceNingbo315201China Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology School of Pharmaceutical SciencesXiamen UniversityXiamen361102China hangzhou Cancer Institute Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang ProvinceAffiliated Hangzhou Cancer HospitalZhejiang University School of MedicineHangzhou310002China Shandong Provincial Key Laboratory of Molecular Engineering School of Chemistry and Pharmaceutical EngineeringQilu University of Technology(Shandong Academy of Sciences)Jinan250353China Department of Materials Science and Engineering National University of SingaporeSingapore117575Singapore

Dry eye is a common ocular disease that results in discomfort and impaired vision,impacting an individual’s quality of life.A great number of drugs administered in eye drops to treat dry eye are poorly soluble in water and are rapidly eliminated from the ocular surface,which limits their therapeutic effects.Therefore,it is imperative to design a novel drug delivery system that not only improves the water solubility of the drug but also prolongs its retention time on the ocular surface.Herein,we develop a copolymer from mono-functional POSS,PEG,and PPG(MPOSS-PEG-PPG,MPEP)that exhibits temperature-sensitive sol-gel transition behavior.This thermo-responsive hydrogel improves the water solubility of FK506 and simultaneously provides a mucoadhesive,long-acting ocular delivery system.In addition,the FK506-loaded POSS hydrogel possesses good biocompatibility and significantly improves adhesion to the ocular surface.In comparison with other FK506 formulations and the PEG-PPG-FK506(F127-FK506)hydrogel,this novel MPOSS-PEG-PPG-FK506(MPEP-FK506)hydrogel is a more effective treatment of dry eye in the murine dry eye model.Therefore,delivery of FK506 in this POSS hydrogel has the potential to prolong drug retention time on the ocular surface,which will improve its therapeutic efficacy in the management of dry eye.

关键词： Ocular adhesive Polyhedral oligomeric silsesquioxane FK506 Dry eye

Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant Monoclonal Antibodies that Target Nav1.7

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Neuroscience Bulletin 2018年第1期34卷 22-41页

作者： Sangsu Bang Jiho Yoo Xingrui Gong Di Liu Qingjian Han Xin Luo Wonseok Chang Gang Chen Sang-Taek Im Yong Ho Kim Judith A.Strong Ma-Zhong Zhang Jun-Ming Zhang Seok-Yong Lee Ru-Rong Ji Department of Anesthesiology Duke University Medical Center Department of Biochemistry Duke University Medical Center Pain Research Center Department of AnesthesiologyUniversity of Cincinnati Department of Anesthesiology Shanghai Children's Medical Center Department of Physiology and Biophysics College of Medicine Eulji University Department of Physiology College of Medicine Gachon University

Abstract The voltage-gated Na＋ channel subtype Nav1.7 is important for pain and itch in rodents and humans. We previously showed that a Nav1.7-targeting monoclonal antibody （SVmab） reduces Na＋ currents and pain and itch responses in mice. Here, we investigated whether recom- binant SVmab （rSVmab） binds to and blocks Nav1.7 similar to SVmab. ELISA tests revealed that SVmab was capable of binding to Nav1.7-expressing HEK293 cells, mouse DRG neurons, human nerve tissue, and the voltagesensor domain II of Nav1.7. In contrast, rSVmab showed no or weak binding to Nav1.7 in these tests. Patch-clamp recordings showed that SVmab, but not rSVmab, markedly inhibited Na＋ currents in Nav1.7-expressing HEK293 cells. Notably, electrical field stimulation increased the blocking activity of SVmab and rSVmab in Nav1.7- expressing HEK293 cells. SVmab was more effective than rSVmab in inhibiting paclitaxel-induced mechanical allodynia. SVmab also bound to human DRG neurons and inhibited their Na＋ currents. Finally, potential reasons for the differential efficacy of SVmab and rSVmab and future directions are discussed.

关键词： Primary afferent neuron Patch clamping （electrophysiology） Sodium channels Animal models

海藻酸钠基pH响应性聚合物胶束的制备及释药性能研究

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中国造纸学报 2021年第2期36卷 18-23页

作者：韩俊源王玉珑刘庆坚刘艳新长沙理工大学化学与食品工程学院湖南长沙410114

采用1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)对海藻酸钠进行改性并制备了海藻酸钠基p H响应性聚合物胶束(以下简称聚合物胶束),分析了EDC/NHS改性前后海藻酸钠的红外光谱(FT-IR)、临界胶束浓度(CMC)、粒... 详细信息

采用1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)对海藻酸钠进行改性并制备了海藻酸钠基p H响应性聚合物胶束(以下简称聚合物胶束),分析了EDC/NHS改性前后海藻酸钠的红外光谱(FT-IR)、临界胶束浓度(CMC)、粒径和Zeta电位;利用反溶剂重结晶法将疏水药物金雀异黄酮包裹于聚合物胶束中,并对载药聚合物胶束的包封率、载药量和pH响应性进行了研究。结果表明,经EDC/NHS改性后的海藻酸钠可在水中形成聚合物胶束,其CMC为0.041 mg/mL;当聚合物胶束与金雀异黄酮质量比为10∶1时,聚合物胶束的包封率和载药量分别为74.4%和8.6%;体外溶出实验表明,载药聚合物胶束具有良好的pH响应性,其在酸性条件(pH值=2.0)下相对稳定,在中性条件(pH值=7.4)下能释放药物,其有望用作口服型肠吸收药物的载体,以实现药物的缓释和控释。

关键词：海藻酸钠聚合物胶束 pH响应性生物质材料药物释放

纳米微晶纤维素/槲皮素复合物的制备及抗氧化和抗癌性能的研究

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中国造纸学报 2020年第1期35卷 7-14页

作者：刘庆坚王玉珑韩俊源刘艳新长沙理工大学化学与食品工程学院湖南长沙410114 中南林业科技大学材料科学与工程学院湖南长沙410004

以纳米微晶纤维素(CNC)为载体,采用反溶剂重结晶法制备了CNC/槲皮素纳米复合物;采用傅里叶变换红外光谱(FTIR)、透射电子显微镜(TEM)、X射线衍射仪(XRD)等对CNC/槲皮素纳米复合物进行表征,并对纳米复合物中槲皮素的溶出性能、抗氧化活性... 详细信息

以纳米微晶纤维素(CNC)为载体,采用反溶剂重结晶法制备了CNC/槲皮素纳米复合物;采用傅里叶变换红外光谱(FTIR)、透射电子显微镜(TEM)、X射线衍射仪(XRD)等对CNC/槲皮素纳米复合物进行表征,并对纳米复合物中槲皮素的溶出性能、抗氧化活性(羟基自由基和1,1-二苯基-2-苦基肼(DPPH)自由基的清除率)和抗癌性能(HepG2肝癌细胞,MTT比色法)进行研究。结果表明,形成CNC/槲皮素纳米复合物后,槲皮素被充分纳米化和稳定分散,由结晶态转变为无定形态;槲皮素的溶出性能得到显著提升,溶解120 min时,槲皮素的溶出度达到92.6%(槲皮素原料溶解120 min时的溶出度仅为4.7%);体外抗氧化活性和HepG2细胞毒性研究表明,形成CNC/槲皮素纳米复合物后,槲皮素的羟基自由基和DPPH自由基清除率得到显著提高,抗癌效果得到明显改善。

关键词：纳米微晶纤维素(CNC) 槲皮素纳米复合物溶出度抗氧化活性抗癌性能

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双环箍套筒式装配节点滞回性能研究

低温建筑技术 2022年第10期44卷 107-111页

作者：赵琦坤杨晓明韩孝坤刘凯侯庆建青岛理工大学山东青岛266011

文中提出一种适用于装配式钢结构的双环箍式套筒节点,为研究新型套筒节点的力学性能,通过改变梁端套筒板处与钢管柱厚度和与梁翼缘厚度的比例关系、套筒与钢管柱间的摩擦系数设计了两组构件,应用ABAQUS进行有限元模拟分析。结果表明套... 详细信息

文中提出一种适用于装配式钢结构的双环箍式套筒节点,为研究新型套筒节点的力学性能,通过改变梁端套筒板处与钢管柱厚度和与梁翼缘厚度的比例关系、套筒与钢管柱间的摩擦系数设计了两组构件,应用ABAQUS进行有限元模拟分析。结果表明套筒与钢管厚度之和与梁翼缘厚度比越大,承载能力越大,耗能越好;摩擦系数对节点性能影响很小。双环箍套筒节点受力性能良好,有较理想的破坏形态,为新型套筒节点在装配式钢结构体系中应用奠定基础。

关键词：装配式钢结构套筒式节点有限元分析滞回曲线摩擦耗能

广西地区汉族2型糖尿病患者脂联素基因多态性研究

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中国药师 2014年第4期17卷 540-543页

作者：苏庆建陈青云南宁市第二人民医院内分泌科南宁530031

目的：研究脂联素基因外显子2单核苷酸多态性（SNP）＋45T→G多态性与广西地区汉族2型糖尿病（T2DM）、肥胖及胰岛素抵抗（ IR）的关系。方法：运用聚合酶链反应-限制性片段长度多态性（ PCR-RFLP）的方法,对313例无亲缘关系的广西地区... 详细信息

目的：研究脂联素基因外显子2单核苷酸多态性（SNP）＋45T→G多态性与广西地区汉族2型糖尿病（T2DM）、肥胖及胰岛素抵抗（ IR）的关系。方法：运用聚合酶链反应-限制性片段长度多态性（ PCR-RFLP）的方法,对313例无亲缘关系的广西地区汉族人群的正常糖耐量（NGT95例）、糖耐量减低（IGT103例）和T2DM115例检测脂联素基因外显子2的多态性,并与代谢值进行相关性研究。结果：广西地区汉族人群中含脂联素基因外显子2的SNP＋45GG基因型的人群高密度脂蛋白（ HDL-C）水平低于TT、TG基因型的人群（P〈0.05）,HOMA-IR高组的GG基因型相对于TT基因型的OR为2.876（95%CI：1.141~7.248）,即GG基因型发生IR的相对危险度是TT基因型的2.876倍。结论：汉族人群中,脂联素基因SNPs＋45 TT基因型的HDL-C较GG基因型频率高；GG基因型较TT基因型人群易产生IR。但汉族人群SNPs＋45基因型与T2DM及肥胖无关。

关键词：脂联素基因多态性外显子2 2型糖尿病