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左心室辅助装置的发展历程、并发症及未来展望

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重庆医学 2024年第9期53卷 1391-1396页

作者：罗渝翔吴庆琛帖红涛重庆医科大学附属第一医院胸外科/心脏大血管外科重庆400016

左心室辅助装置(LVAD)是终末期心力衰竭有效的治疗手段之一,发展迅速,应用前景广阔。出血、感染、卒中、右心衰及心律失常是LVAD植入术后需要关注的常见并发症。该文对LVAD的发展历程、并发症及未来展望进行总结,旨在持续优化及推广LVAD。

关键词：心力衰竭机械循环支持心室辅助装置人工心脏并发症综述

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Maximizing the linear and nonlinear optical responses of alkaline tricyanomelaminate

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Fundamental Research 2023年第6期3卷 974-978页

作者： Yanqiang Li qingchen wu Zheshuai Lin Youchao Liu Yang Zhou Xin Chen Minjuan Li Maochun Hong Junhua Luo Sangen Zhao State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China Technical Institute of Physics and Chemistry Chinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China Fuzhou 350108China

High-performance bi-functional materials are in urgent demand for the next-generation integrated optical *** this work,we successfully synthesized the first tricyanomelaminate with bi-functional optical responses,namely Cs_(3)C_(6)N_(9)•H_(2)O(I),from its analogue Na_(3)C_(6)N_(9)•3H_(2)O by a facile ion exchange *** contrast to Na_(3)C_(6)N_(9)•3H_(2)O,I realizes an optimal arrangement of𝜋π-conjugated(C_(6)N_(9))3−anion groups in its crystal *** a result,the second-order nonlinear optical(NLO)response is greatly enhanced from nearly zero of Na_(3)C_(6)N_(9)•3H_(2)O to∼9.8×KH_(2)PO_(4)of ***,I exhibits a giant linear optical anisotropic response(***)of 0.52 at the wavelength of 550 *** responses are almost the largest among the inorganic compounds ofπ-conjugated rings,which indicates that I has great potential as a bi-functional optical *** and theoretical analyses reveal the microscopic origin of excellent optical *** work would attract a lot of interest to the persistently neglected potential of tricyanomelaminates as linear optical and NLO crystals.

关键词： Nonlinear optical response Second harmonic generation Birefringence Tricyanomelaminate Structure-property relationship Inorganic chemistry

Sema3A secreted by sensory nerve induces bone formation under mechanical loads

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International Journal of Oral Science 2024年第1期16卷 62-72页

作者： Hongxiang Mei Zhengzheng Li Qinyi Lv Xingjian Li Yumeng wu qingchen Feng Zhishen Jiang Yimei Zhou Yule Zheng Ziqi Gao Jiawei Zhou Chen Jiang Shishu Huang Juan Li State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology Sichuan UniversityChengduChina Department of Orthopedic Surgery and Orthopedic Research Institute West China HospitalSichuan UniversityChengduChina

Bone formation and deposition are initiated by sensory nerve infiltration in adaptive bone remodeling. Here, we focused on the role of Semaphorin 3A(Sema3A), expressed by sensory nerves, in mechanical loads-induced bone formation and nerve withdrawal using orthodontic tooth movement(OTM) model. Firstly, bone formation was activated after the 3rd day of OTM,coinciding with a decrease in sensory nerves and an increase in pain threshold. Sema3A, rather than nerve growth factor(NGF),highly expressed in both trigeminal ganglion and the axons of periodontal ligament following the 3rd day of OTM. Moreover, in vitro mechanical loads upregulated Sema3A in neurons instead of in human periodontal ligament cells(hPDLCs) within 24 ***, exogenous Sema3A restored the suppressed alveolar bone formation and the osteogenic differentiation of hPDLCs induced by mechanical overload. Mechanistically, Sema3A prevented overstretching of F-actin induced by mechanical overload through ROCK2 pathway, maintaining mitochondrial dynamics as mitochondrial fusion. Therefore, Sema3A exhibits dual therapeutic effects in mechanical loads-induced bone formation, both as a pain-sensitive analgesic and a positive regulator for bone formation.

关键词： Sema3A formation overload

Identification of cross-reactive CD8^(+)T cell receptors with high functional avidity to a SARS-CoV-2 immunodominant epitope and its natural mutant variants

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Genes & Diseases 2022年第1期9卷 216-229页

作者： Chao Hu Meiying Shen Xiaojian Han Qian Chen Luo Li Siyin Chen Jing Zhang Fengxia Gao Wang Wang Yingming Wang Tingting Li Shenglong Li Jingjing Huang Jianwei Wang Ju Zhu Dan Chen qingchen wu Kun Tao Da Pang Aishun Jin Department of Immunology College of Basic MedicineChongqing Medical UniversityChongqing 400016PR China Chongqing Key Laboratory of Cancer Immunology Translational Medicine Chongqing Medical UniversityChongqing 400016PR China Department of Breast Surgery Harbin Medical University Cancer HospitalHarbinHeilongjiang 150081PR China Department of Cardiothoracic Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016PR China

Despite the growing knowledge of T cell responses in COVID-19 patients,there is a lack of detailed characterizations for T cell-antigen interactions and T cell ***,with a predicted peptide library from SARS-CoV-2 S and N proteins,we found that specific CD8+T cell responses were identified in over 75%of COVID-19 convalescent patients(15/20)and an epitope from the N protein,N361-369(KTFPPTEPK),was the most dominant epitope from our selected peptide ***,we discovered 2 N361-369-specific T cell receptors(TCRs)with high functional avidity that were independent of the CD8 *** TCRs exhibited complementary cross-reactivity to several presently reported N361-369 mutant variants,as to the wild-type ***,the natural functions of these TCRs in the cytotoxic immunity against SARS-CoV-2 were determined with dendritic cells(DCs)and the lung organoid *** found that the N361-369 epitope could be normally processed and endogenously presented by these different types of antigen presenting cells,to elicit successful activation and effective cytotoxicity of CD8+T cells ex *** study evidenced potential mechanisms of cellular immunity to SARS-CoV-2,and illuminated potential ways of viral clearance in COVID-19 *** results indicate that utilizing CD8-independent TCRs against SARS-CoV-2-associated antigens may provide functional superiority that is beneficial for the adoptive cell immunotherapies based on natural or genetically engineered T ***,this information is highly relevant for the development of the next-generation vaccines with protections against continuously emerged SARS-CoV-2 mutant strains.

关键词： CD8^(+)T cell HLA class I Lung organoid SARS-CoV-2 T cell epitope TCR

Covert Wireless Communication on Beidou Short Message Communication

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Covert Wireless Communication on Beidou Short Message Commun...

敲减FBXL20基因降低食管癌KYSE150细胞增殖

第十三届中国卫星导航年会

作者： Maochun Wang Weiwei Yang Lu Xu Xin Lv Ye Chen qingchen wu Bing Liu College of Communications Engineering Army Engineering University of PLA Beijing Satellite Navigation Center

As a significant feature and advantage of BeiDou navigation satellite system（BDS）,short message communication（SMC） with the character of wide coverage and low communication cost,which is widely used in transportation,fishery,agriculture and forestry ***,due to the broadcast nature of wireless transmission,the security issue of SMC cannot be *** existing security systems and research are mostly about encryption algorithms,without considering the protection of communication behavior and the transmitter’s *** this background,we explore the covert wireless communication to guarantee the security of BeiDou ***,the warden’s detection strategy is analyzed through hypothesis testing ***,the covertness of SMC is discussed in two scenarios:whether the user’s location information is exposed or *** simulation results indicate that the user’s SMC behavior can be effectively protected by using the jammer with random transmit power.

关键词： Beidou short message Physical layer security Covert wireless communication

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基因组学与应用生物学 2018年第4期37卷 1661-1666页

作者：何枫陈小龙吴庆琛重庆医科大学附属第一医院

为了探究FBXL20（F-box and leucine rich repeat protein 20）基因对人类食管癌细胞株KYSE150的影响及机制,我们采用慢病毒转染技术,构建FBXL20基因的慢病毒载体,转染KYSE150细胞系;采用实时荧光定量PCR和Western blotting检测细胞株中... 详细信息

为了探究FBXL20（F-box and leucine rich repeat protein 20）基因对人类食管癌细胞株KYSE150的影响及机制,我们采用慢病毒转染技术,构建FBXL20基因的慢病毒载体,转染KYSE150细胞系;采用实时荧光定量PCR和Western blotting检测细胞株中FBXL20基因m RNA和蛋白的表达量;采用Western blotting检测细胞株β-catenin、cyclin D1、c-myc的蛋白水平;采用CCK-8法检测细胞增殖能力;采用流式细胞术检测细胞周期与凋亡。成功构建低表达FBXL20慢病毒转染KYSE150细胞后,与对照组（CON组）相比,低表达FBXL20组（FBXL20-RNAi组）的KYSE150细胞增殖能力显著降低,细胞被阻滞在G1期,凋亡能力显著增加,β-catenin、cyclin D1和c-myc蛋白水平显著降低。我们的实验证实FBXL20在人类食管癌中是一个致癌基因,通过激活Wnt通路促进癌症的发生和发展。

关键词： FBXL20 食管癌 Wnt通路周期增殖凋亡

LATS1在食管鳞癌中的表达及启动子甲基化状态研究

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基因组学与应用生物学 2018年第6期37卷 2751-2756页

作者：陈小龙何枫吴庆琛重庆医科大学附属第一医院

在我们以前的研究中,在食管鳞癌细胞株中过表达大肿瘤抑制基因-1（large tumor suppressor kinase 1,LATS1）能够明显抑制细胞增殖、细胞迁移和侵袭,同时能够促进细胞凋亡。为了进一步研究LATS1基因在食管鳞状细胞癌发生发展中的作用以... 详细信息

在我们以前的研究中,在食管鳞癌细胞株中过表达大肿瘤抑制基因-1（large tumor suppressor kinase 1,LATS1）能够明显抑制细胞增殖、细胞迁移和侵袭,同时能够促进细胞凋亡。为了进一步研究LATS1基因在食管鳞状细胞癌发生发展中的作用以及可能的机制,我们在食管鳞癌组织与配对癌旁组织中,应用免疫组化检测LATS1基因蛋白质表达情况,应用荧光定量PCR（qRT-PCR）检测配对组织LATS1基因mRNA表达差异。甲基化特异性PCR（MSP）和亚硫酸氢盐测序法（BGS）检测食管鳞癌组织及细胞中LATS1基因的CpG岛甲基化状态。我们发现LATS1基因在癌旁组织中广泛表达,而癌组织中LATS1基因表达明显下调。结果表明LATS1在食管鳞癌中起到抑癌基因的作用,并且与肿瘤的临床分期相关,而其下调与CpG岛的高甲基化无关。在食管鳞癌细胞中过表达LATS1抑制细胞增殖、细胞迁移和侵袭,同时能够促进细胞凋亡。因此,LATS1基因值得进一步探索作为食管鳞癌生物标志物的可能用途和未来分子诊断和治疗的目标。

关键词：食管鳞状细胞癌 LATS1 DNA甲基化

Occurrences of Glucocorticoids in Aquatic Environment and Their Removal during Wastewater Treatment

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Journal of Donghua University(English Edition) 2023年第3期40卷 293-318页

作者：吴清晨吴世闵张茵茵 ANDERE Clement Miruka 朱大海张艾刘亚男薛罡 College of Environmental Science and Engineering Donghua UniversityShanghai 201620China Department of Chemical and Environmental Engineering University of ArizonaArizona AZ 85721-0011United States

Glucocorticoids(GCs) are a group of endocrine-disrupting compounds(EDCs) frequently prescribed against various medical ***,GCs have been shown to be effective in managing septic shock in patients infected with the 2019 novel coronavirus(COVID-19).Due to colossal consumption and potential risks to aquatic organisms,GCs have immensely attracted the focus of the scientific research community as a water ***,the aim of this paper is to review the current knowledge on the occurrence of various GCs in the aquatic environment and their removal during wastewater treatment.A variety of GCs are ubiquitous in surface water,hospital wastewater,and sewage water *** the minimum concentration in volume is below 0.01 ng/L,and the maximum one is 10 000 ng/L,and enter the environment through hospital and urban wastewater *** with natural GCs,higher risks to aquatic environments could be induced by synthetic *** current activated sludge processes used in wastewater treatment plants(WWTPs) are not fully effective in eliminating GCs,some of which may further increase the risk of GC in the *** comparison with the aerobic process in WWTPs,the anaerobic and anoxic processes were found to be more efficient for GC *** the studied GCs,fluticasone propionate,clobetasol propionate,fluocinolone acetonide,and triamcinolone acetonide need more attention due to their low removal efficiencies and strong *** the advanced treatment processes,reverse osmosis,ultraviolet irradiation,CaO_(2),and plasma could achieve significant GC activity removal while micro/ultra-filtration,chlorination,and ozonation were less efficient.

关键词： glucocorticoid(GC) endocrine disrupting compound(EDC) wastewater treatment plant(WWTP) advanced wastewater treatment

Lnc-TMEM132D-AS1高表达明显降低非小细胞肺癌对奥希替尼的敏感性

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南方医科大学学报 2023年第2期43卷 242-250页

作者：赵齐林王楠李亚霁吴庆琛吴兰香重庆医科大学附属第一医院心胸外科重庆400016 重庆医科大学生命科学研究院重庆400016

目的建立奥希替尼获得性耐药非小细胞肺癌(NSCLC)细胞模型,筛选耐药前后差异表达的长链非编码RNA(lncRNAs),并探讨筛选的lncRNA在奥希替尼获得性耐药中的作用及分子机制。方法奥希替尼获得性耐药细胞株H1975_OR和HCC827_OR是由奥希替尼... 详细信息

目的建立奥希替尼获得性耐药非小细胞肺癌(NSCLC)细胞模型,筛选耐药前后差异表达的长链非编码RNA(lncRNAs),并探讨筛选的lncRNA在奥希替尼获得性耐药中的作用及分子机制。方法奥希替尼获得性耐药细胞株H1975_OR和HCC827_OR是由奥希替尼敏感的NSCLC细胞株H1975和HCC827(分别命名为H1975_S和HCC827_S)建立而来;通过CCK-8、克隆形成及流式凋亡实验检测细胞对奥希替尼的敏感性;RNA测序(RNA-seq)、实时荧光定量PCR(qPCR)技术筛选耐药前后差异表达的lncRNAs;采用CCK-8、克隆形成及Transwell实验检测所筛选的lncRNA在NSCLC细胞奥希替尼获得性耐药中的作用;通过核质分离、生物信息学分析及qPCR技术观察该lncRNA的亚细胞定位,并探索其下游互作分子。结果H1975_OR与HCC827_OR对奥希替尼的耐药指数分别为598.70和428.82(P<0.001)。耐药株的生长增殖与克隆形成能力显著提高,而凋亡率降低(P<0.01)。RNA-seq提示H1975_OR与H1975_S之间以及HCC827_OR与HCC827_S之间有共同差异表达的lncRNAs 34个。q PCR验证发现,lnc-TMEM132D-AS1在耐药性NSCLC细胞株中升高最为显著(P<0.01)。TCGA数据库分析提示,lnc-TMEM132D-AS1在NSCLC组织中的表达水平高于癌旁组织(P<0.001),且与患者预后不良相关(P<0.05)。LncTMEM132D-AS1过表达可提高奥希替尼处理下敏感株的生长增殖、克隆形成及迁移能力(P<0.05),而敲低其表达水平可部分恢复耐药株的奥希替尼敏感性(P<0.01)。lnc-TMEM132D-AS1主要定位在胞质,生物信息学分析提示hsa-miR-766-5p是其潜在靶点,两者表达水平呈负相关(P<0.01)。对hsa-miR-766-5p下游的靶mRNAs分析提示相关基因主要集中于Ras信号通路。结论Lnc-TMEM132D-AS1在奥希替尼获得性耐药NSCLC细胞株中表达显著上调,明显降低了NSCLC细胞对奥希替尼的敏感性,是NSCLC奥希替尼获得性耐药的潜在生物标志物及治疗靶标。

关键词：长链非编码RNA lnc-TMEM132D-AS1 NSCLC 奥希替尼获得性耐药