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Modulation of starch synthesis in Arabidopsis via phytochrome B-mediated light signal transduction

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Journal of Integrative Plant Biology 2024年第5期66卷 973-985页

作者： qingbiao shi Ying Xia Na Xue Qibin Wang Qing Tao Mingjing Li Di Xu Xiaofei Wang Fanying Kong Haisen Zhang Gang Li National Key Laboratory of Wheat Improvement College of Life SciencesShandong Agricultural UniversityTai'an 271018China National Key Laboratory of Wheat Improvement College of AgronomyShandong Agricultural UniversityTai'an 271018China

Starch is a major storage carbohydrate in plants and is critical in crop yield and *** synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise molecular mechanisms governing this process remain largely *** this study,we revealed that high red to far-red(high R:FR)light significantly induces the synthesis of leaf starch and the expression of synthesis-related genes,whereas low R:FR light suppress these *** phytochrome B(phyB),the primary R and FR photoreceptor,was identified as a critical positive regulator in this *** of phyB,basic leucine zipper transcription factor ELONGATED HYPOCOTYL5(HY5)was found to enhance starch synthesis,whereas the basic helix-loop-helix transcription factors PHYTOCHROME INTERACTING FACTORs(PIF3,PIF4,and PIF5)inhibit starch synthesis in Arabidopsis ***,HY5 and PIFs directly compete for binding to a shared G-box cis-element in the promoter region of genes encoding starch synthases GBSS,SS3,and SS4,which leads to antagonistic regulation of their expression and,consequently,starch *** findings highlight the vital role of phyB in enhancing starch synthesis by stabilizing HY5 and facilitating PIFs degradation under high R:FR light ***,under low R:FR light,PIFs predominantly inhibit starch *** study provides insight into the physiological and molecular functions of phyB and its downstream transcription factors HY5 and PIFs in starch synthesis regulation,shedding light on the regulatory mechanism by which plants synchronize dynamic light signals with metabolic cues to module starch synthesis.

关键词： Arabidopsis thaliana HY5 light signal phyB PIFs starch synthesis

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Histological and single-nucleus transcriptome analyses reveal the specialized functions of ligular sclerenchyma cells and key regulators of leaf angle in maize

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Molecular Plant 2024年第6期17卷 920-934页

作者： Qibin Wang Qiuyue Guo qingbiao shi Hengjia Yang Meiling Liu Yani Niu Shuxuan Quan Di Xu Xiaofeng Chen Laiyi Li Wenchang Xu Fanying Kong Haisen Zhang Pinghua Li Bosheng Li Gang Li State Key Laboratory of Wheat Improvement College of Life SciencesShandong Agricultural UniversityTai’an 271018China Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agriculture Sciences in WeifangWeifangShandong 261325China State Key Laboratory of Wheat Improvement College of AgronomyShandong Agricultural UniversityTai’an 271018China

Leaf angle(LA)is a crucial factor that affects planting density and yield in ***,the regulatory mechanisms underlying LA formation remain largely *** this study,we performed a comparative histological analysis of the ligular region across various maize inbred lines and revealed that LA is significantly influenced by a two-step regulatory process involving initial cell elongation followed by subsequent lignification in the ligular adaxial sclerenchyma cells(SCs).Subsequently,we performed both bulk and single-nucleus RNA sequencing,generated a comprehensive transcriptomic atlas of the ligular region,and identified numerous genes enriched in the hypodermal cells that may influence their specialization into ***,we functionally characterized two genes encoding atypical basic-helix-loop-helix(bHLH)transcription factors,bHLH30 and its homolog bHLH155,which are highly expressed in the elongated adaxial *** analyses revealed that bHLH30 and bHLH155 positively regulate LA expansion,and molecular experiments demonstrated their ability to activate the transcription of genes involved in cell elongation and lignification of *** findings highlight the specialized functions of ligular adaxial SCs in LA regulation by restricting further extension of ligular cells and enhancing mechanical *** transcriptomic atlas of the ligular region at single-nucleus resolution not only deepens our understanding of LA regulation but also enables identification of numerous potential targets for optimizing plant architecture in modern maize breeding.

关键词： planting specialized angle

Regulation of Leaf Angle by Auricle Development n Maize

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Molecular Plant 2017年第3期10卷 516-519页

作者： Fanying Kong Tingting Zhang Jisheng Liu Siqi Heng qingbiao shi Haisen Zhang Zeli Wang Lei Ge Pinghua Li Xiaoduo Lu Gang Li College of Life Science State Key Laboratory of Crop Biology Shandong AgricuLtural University Tai'an Shandong 271018 China College of Agronomy Shandong Agricultural University Tai'an Shandong 271018 China Institute of Molecular Breeding for Maize Qilu Normal University Jinan Shandong 250200 China These authors contributed equally to this article.

In plants, the leaf angle （LA） affects how densely crops can be planted and thus is one of factors determining grain yield per acre. This complex quantitative trait is controlled by developmental signals, hormones, ... 详细信息

关键词： Regulation Leaf Angle Auricle Development in Maize

FUP1——AN UNIFIED PROGRAM FOR CALCULATING ALL FAST NEUTRON DATA OF FISSILE NUCLEUS

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中国核科技报告 1990年第S2期 31-33页

作者： Cai Chonghai Zuo Yixin (NANKAI UNIVERSITY, TIANJIN)Wang Zhengxing(PEKING UNIVERSITY, BEIJING) Wang Shunuan Yan shiwei Shen qingbiao shi XiangjunSu Zongdi Tian Ye Zhang Jingshang (INSTITUTE OF ATOMIC ENERGY, BEIJING)

FUP1 is the first edition of an unified program for calculating all the fastneutron data(10 keV～20 MeV)in ENDF/B—4 format for fissile nucleus,it isdesigned on the base of MUP2 code which is the second edition of an unifiedprogram for theoretical calculation of fast neutron data of medium—heavy un-cleus by using optical model,Hauser—Feshbach theory with width fluctuationcorrection(WHF)and pre—equilibrium(PE)statistical theory based on excitonmodel.

关键词： FUP1 AN UNIFIED PROGRAM FOR CALCULATING ALL FAST NEUTRON DATA OF FISSILE NUCLEUS FAST ENDF

Phylogenetic position of Ephedra rhytidosperma,a species endemic to China:Evidence from chloroplast and ribosomal DNA sequences

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Chinese Science Bulletin 2005年第24期50卷 2901-2904页

作者： WANG qingbiao WANG Li ZHOU Renchao ZHAO Xiaoming shi Suhua YANG Yong ZHONG Yang Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering School of Life Sciences Fudan University Shanghai 200433 China State Key Laboratory for Biocontrol School of Life Sciences Sun Yat-sen University Guangzhou 510275 China Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China Shanghai Center for Bioinformation Technology Shanghai 201203 China

The chloroplast genes matK and rbcL, ribo- somal gene 18S and ITS regions of nuclear ribosomal DNA from Ephedra rhytidosperma, a species endemic to China, were sequenced and its phylogenetic position was investi- gated. Independent and combined phylogenetic analyses for the DNA sequences from 16 taxa representing 15 species of the genus Ephedra were performed using the maximum par- simony (MP), neighbor-joining (NJ), minimum evolution (ME) and maximum likelihood (ML) methods. The results indicate that E. rhytidosperma is closely related to E. equiset- ina. The divergence time between them is estimated to be 10.85±2.44 Ma based on the results of the relative-rate tests and the evolutionary rate of rbcL gene.

关键词：麻黄属植物中国叶绿体核糖体 DNA序列系统发育学

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Radiation protection designs of the linac for the HEPS

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Radiation Detection Technology and Methods 2021年第4期5卷 576-585页

作者： Qiongyao Liu Zhongjian Ma qingbiao Wu Haoyu shi Gang Zhang Huijie Zhang Pingcheng Liu Qingbin Wang Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Spallation Neutron Source Science Center Dongguan 523803China University of Chinese Academy of Sciences Beijing 100049China Shandong Institute of Advanced Technology Shandong 250000China

Purpose The high-energy photon source(HEPS)is the first fourth-generation light source under construction in *** is designed to operate at an average current of 200 mA stored beam current with a top-up model at 6 GeV *** the linac radiation shielding design,a suitable beam loss scenario,optimized thickness for the bulk shielding and detailed structure design for dumps should be *** this paper,the beam loss scenarios were determined and categorized as normal;the dose limits were presented;using these scenarios and the dose limits,the thickness of the linac tunnel was calculated and detailed designs of the main beam dumps were *** material selection and size setting of the low-power electron beam dump were *** The Monte Carlo code is a good choice to simulate the radiation *** the ishiELD11 was used to verify the simulation *** and conclusion The designs of the linac bulk shield and dumps satisfied the requirements of radiation protection.

关键词： HEPS,Linac Radiation shielding design Dump FLUKA ishiELD11

Phylogenetic relationships and divergence times of the family Araucariaceae based on the DNA sequences of eight genes

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Chinese Science Bulletin 2009年第15期54卷 2648-2655页

作者： LIU Nian ZHU Yong WEI ZongXian CHEN Jie WANG qingbiao JIAN ShuGuang ZHOU DangWei shi Jing YANG Yong ZHONG Yang Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering School of Life Sciences Fudan University Shanghai 200433 China Lushan Botanical Garden Chinese Academy of Sciences Lushan 332900 China Shanghai Center for Bioinformation Technology Shanghai 200235 China South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Araucariaceae is one of the most primitive families of the living conifers, and its phylogenetic rela-tionships and divergence times are critically important issues. The DNA sequences of 8 genes, i.e., nuclear ribosomal 18S and 26S rRNA, chloroplast 16S rRNA, rbcL, matK and rps4, and mitochondrial coxI and atp1, obtained from this study and GenBank were used for constructing the molecular phylogenetic trees of Araucariaceae, indicating that the phylogenetic relationships among the three genera of this family should be ((Wollemia, Agathis), Araucaria). On the basis of the fossil calibrations of Wol-lemia and the two tribes Araucaria and Eutacta of the genus Araucaria, the divergence time of Arauca-riaceae was estimated to be (308 ± 53) million years ago, that is, the origin of the family was in the Late Carboniferous rather than Triassic as a traditional view. With the same gene combination, the diver-gence times of the genera Araucaria and Agathis were (246 ± 47) and (61 ± 15) Ma, respectively. Statistical analyses on the phylogenetic trees generated by using different genes and comparisons of the divergence times estimated by using those genes suggested that the chloroplast matK and rps4 genes are most suitable for investigating the phylogenetic relationships and divergence times of the family Araucariaceae.

关键词：系统发育关系 DNA序列南洋杉基因基础分歧家庭

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Study on Influence of Vertical Position of Steel Bracing in Foundation Pit of Open Cut Railway on Deformation of Envelope Structure

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Study on Influence of Vertical Position of Steel Bracing in ...

Study on Dust Movement in Long Tunnel during Blasting Period

作者： qingbiao Wang Pengcheng Ma Zhenyue shi School of Civil Engineering and Architecture Shandong University of Science and Technology Key Laboratory of Civil Engineering Disaster Prevention and Mitigation of Shandong Province Shandong University of Science and Technology

The influence of different vertical position of steel support on the retaining structure of deep foundation pit is studied,and the deformation and deformation of retaining pile under multi-supported support conditions are revealed,which provides support design and excavation optimization design for deep foundation pit *** accordance *** the calculation of the rational deep foundation pit calculation software and FLAC3D numerical simulation software,the deformation law of the retaining structure under different vertical position of steel support is *** research results are as follows:（1） Different vertical positions of the steel support to the retaining pile the deep horizontal displacement of the pile has a great *** encryption of the lower steel support is conducive to the stability of the retaining structure.（2） The influence of the vertical position change of the steel support on the horizontal displacement of the pile top is limited,but the first two supports are *** the position is unchanged,the third steel support is moved down by 1 m,which can effectively limit the horizontal displacement of the pile top,which is beneficial to the stability of the retaining structure.

关键词： Deep Foundation Pit Engineering Steel Bracing Envelope Structure FLAC3D

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Study on Dust Movement in Long Tunnel during Blasting Period