Phylogenetic relationships and divergence times of the family Araucariaceae based on the DNA sequences of eight genes

作     者：LIU Nian ZHU Yong WEI ZongXian CHEN Jie WANG QingBiao JIAN ShuGuang ZHOU DangWei SHI Jing YANG Yong ZHONG Yang 

作者机构：Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering School of Life Sciences Fudan University Shanghai 200433 China Lushan Botanical Garden Chinese Academy of Sciences Lushan 332900 China Shanghai Center for Bioinformation Technology Shanghai 200235 China South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2009年第54卷第15期

页      面：2648-2655页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 090706[农学-园林植物与观赏园艺] 081704[工学-应用化学] 0907[农学-林学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 

基　　金：Supported by the National Infrastructure of Natural Resources for Science and Technology (Grant No. 2005DKA21403) MOST Basic Science and Technology (Grant No. 2007FY110100) Shanghai Leading Academic Discipline Project (Grant No. B111) Shanghai Science and Technology Committee (Grant No. 07XD14025) the Graduate Students Innovation Foundation of Fudan University (Grant No. EYH1322098) National Science Fund for Fostering Talents in Basic Science (Grant No. J0630643) 

主　　题：系统发育关系 DNA序列 南洋杉 基因 基础 分歧 家庭 

摘      要：Araucariaceae is one of the most primitive families of the living conifers, and its phylogenetic rela-tionships and divergence times are critically important issues. The DNA sequences of 8 genes, i.e., nuclear ribosomal 18S and 26S rRNA, chloroplast 16S rRNA, rbcL, matK and rps4, and mitochondrial coxI and atp1, obtained from this study and GenBank were used for constructing the molecular phylogenetic trees of Araucariaceae, indicating that the phylogenetic relationships among the three genera of this family should be ((Wollemia, Agathis), Araucaria). On the basis of the fossil calibrations of Wol-lemia and the two tribes Araucaria and Eutacta of the genus Araucaria, the divergence time of Arauca-riaceae was estimated to be (308 ± 53) million years ago, that is, the origin of the family was in the Late Carboniferous rather than Triassic as a traditional view. With the same gene combination, the diver-gence times of the genera Araucaria and Agathis were (246 ± 47) and (61 ± 15) Ma, respectively. Statistical analyses on the phylogenetic trees generated by using different genes and comparisons of the divergence times estimated by using those genes suggested that the chloroplast matK and rps4 genes are most suitable for investigating the phylogenetic relationships and divergence times of the family Araucariaceae.