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Transplantation of erythropoietin gene-modified neural stem cells improves the repair of injured spinal cord

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Neural Regeneration Research 2015年第9期10卷 1483-1490页

作者： Min-fei Wu Shu-quan Zhang Rui Gu Jia-bei Liu Ye Li qing-san zhu Department of Orthopedics the Second Hospital of Jilin University Department of Orthopedics Tianjin Nankai Hospital Department of Orthopedics China-Japan Union Hospital of Jilin University

The protective effects of erythropoietin on spinal Here, the eukaryotic expression plasmid pcDNA3.1 cord injury have not been well described. human erythropoietin was transfected into rat neural stem cells cultured in vitro. A rat model of spinal cord injury was established using a free falling object. In the human erythropoietin-neural stem cells group, transfected neural stem cells were injected into the rat subarachnoid cavity, while the neural stem cells group was inject- ed with non-transfected neural stem cells. Dulbecco＇s modified Eagle＇s medium/F12 medium was injected into the rats in the spinal cord injury group as a control. At 1-4 weeks post injury, the motor function in the rat lower limbs was best in the human erythropoietin-neural stem ceils group, followed by the neural stem cells group, and lastly the spinal cord injury group. At 72 hours, compared with the spinal cord injury group, the apoptotic index and Caspase-3 gene and protein expressions were apparently decreased, and the bd-2 gene and protein expressions were noticeably increased, in the tissues surrounding the injured region in the human erythro- poietin-neural stem cells group. At 4 weeks, the somatosensory evoked potential latencies were cavities were clearly smaller and the motor and remarkably shorter in the human erythropoi- etin-neural stem cells group and neural stem cells group than those in the spinal cord injury group. These differences were particularly obvious in the human erythropoietin-neural stem cells group. More CM-Dil-positive cells and horseradish peroxidase-positive nerve fibers and larger amplitude motor and somatosensory evoked potentials were found in the human erythro- poietin-neural stem cells group and neural stem cells group than in the spinal cord injury group. Again, these differences were particularly obvious in the human erythropoietin-neural stem cells group. These data indicate that transplantation of erythropoietin gene-modified neural stem cells into the su

关键词： nerve regeneration spinal cord injury neural stem cells erythropoietin motor function subarachnoid cavity transplantation injury recovery neural regeneration

Neuroprotective effects of electroacupuncture on early- and late-stage spinal cord injury

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Neural Regeneration Research 2015年第10期10卷 1628-1634页

作者： Min-fei Wu Shu-quan Zhang Jia-bei Liu Ye Li qing-san zhu Rui Gu Department of Orthopedics Bethune Second Hospital of Jilin University Department of Orthopedics Tianjin Nankai Hospital Department of Orthopedics China-Japan Union Hospital Jilin University

Previous studies have shown that the neurite growth inhibitor Nogo-A can cause secondary neural damage by activating Rho A. In the present study, we hypothesized that electroacupuncture promotes neurological functional recovery after spinal cord injury by inhibiting Rho A expression. We established a rat model of acute spinal cord injury using a modification of Allen＇s method. The rats were given electroacupuncture treatment at Dazhui（Du14）, Mingmen（Du4）, sanyinjiao（SP6）, Huantiao（GB30）, Zusanli（ST36） and Kunlun（BL60） acupoints with a sparsedense wave at a frequency of 4 Hz for 30 minutes, once a day, for a total of 7 days. Seven days after injury, the Basso, Beattie and Bresnahan（BBB） locomotor scale and inclined plane test scores were significantly increased, the number of apoptotic cells in the spinal cord tissue was significantly reduced, and Rho A and Nogo-A m RNA and protein expression levels were decreased in rats given electroacupuncture compared with rats not given electroacupuncture. Four weeks after injury, pathological tissue damage in the spinal cord at the site of injury was alleviated, the numbers of glial fibrillary acidic protein- and neurofilament 200-positive fibers were increased, the latencies of somatosensory-evoked and motor-evoked potentials were shortened, and their amplitudes were increased in rats given electroacupuncture. These findings suggest that electroacupuncture treatment reduces neuronal apoptosis and decreases Rho A and Nogo-A m RNA and protein expression at the site of spinal cord injury, thereby promoting tissue repair and neurological functional recovery.

关键词： nerve regeneration spinal cord injury electroacupuncture locomotion Rho A Nogo-A glial fibrillary acidic protein neurofilament 200 neural regeneration

Transplantation of human telomerase reverse transcriptase gene-transfected Schwann cells for repairing spinal cord injury

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Neural Regeneration Research 2015年第12期10卷 2040-2047页

作者： Shu-quan Zhang Min-fei Wu Jia-bei Liu Ye Li qing-san zhu Rui Gu Department of Orthopedics Tianjin Nankai Hospital Department of Spine Surgery Orthopedic HospitalSecond HospitalClinical HospitalJilin University Department of Orthopedics China-Japan Union HospitalJilin University

Transfection of the human telomerase reverse transcriptase（h TERT）gene has been shown to increase cell proliferation and enhance tissue *** the present study,h TERT was transfected into rat Schwann cells.A rat model of acute spinal cord injury was established by the modified free-falling *** PLXSN was injected at the site of spinal cord injury as a vector to mediate h TERT gene-transfected Schwann cells（1×10^（10）/L;10μL）or Schwann cells（1×10^（10）/L;10μL）without h TERT gene *** 1 and 4 weeks after model establishment,motor function of the lower limb improved in the h TERT-transfected group compared with the group with non-transfected Schwann *** deoxynucleotidyl transferase-mediated d UTP nick-end labeling and reverse transcription-polymerase chain reaction results revealed that the number of apoptotic cells,and gene expression of aquaporin 4/9 and matrix metalloproteinase 9/2decreased at the site of injury in both groups;however,the effect improved in the h TERT-transfected group compared with the Schwann cells without h TERT transfection *** and eosin staining,PKH26 fluorescent labeling,and electrophysiological testing demonstrated that compared with the non-transfected group,spinal cord cavity and motor and sensory evoked potential latencies were reduced,while the number of PKH26-positive cells and the motor and sensory evoked potential amplitude increased at the site of injury in the h TERT-transfected *** findings suggest that transplantation of h TERT gene-transfected Schwann cells repairs the structure and function of the injured spinal cord.

关键词： nerve regeneration spinal cord injury Schwann cells transplantation motor function telomerase reverse transcriptase proliferation modification cells neural regeneration

Senegenin inhibits neuronal apoptosis after spinal cord contusion injury

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Neural Regeneration Research 2016年第4期11卷 657-663页

作者： Shu-quan Zhang Min-fei Wu Rui Gu Jia-bei Liu Ye Li qing-san zhu Jin-lan Jiang Department of Orthopedics Nankai Hospital Department of Orthopedics the Second Hospital of Jilin University Department of Orthopedics China-Japan Union HospitalJilin University Scientific Research Center China-Japan Union HospitalJilin University

Senegenin has been shown to inhibit neuronal apoptosis,thereby exerting a neuroprotective *** the present study,we established a rat model of spinal cord contusion injury using the modified Allen＇s *** hours after injury,senegenin（30 mg/g） was injected into the tail vein for 3 consecutive *** reduced the size of syringomyelic cavities,and it substantially reduced the number of apoptotic cells in the spinal *** the site of injury,Bax and Caspase-3 m RNA and protein levels were decreased by senegenin,while Bcl-2 m RNA and protein levels were *** fiber density was increased in the spinal cord proximal to the brain,and hindlimb motor function and electrophysiological properties of rat hindlimb were *** together,our results suggest that senegenin exerts a neuroprotective effect by suppressing neuronal apoptosis at the site of spinal cord injury.

关键词： nerve regeneration spinal cord contusion senegenin thinleaf milkwort root motor function apoptosis electrophysiology neural regeneration

Predictive risk factors for recollapse of cemented vertebrae after percutaneous vertebroplasty: A meta-analysis

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World Journal of Clinical Cases 2021年第12期9卷 2778-2790页

作者： Yi-Hang Ma Zhi-Sen Tian Hao-Chuan Liu Bo-Yin Zhang Yu-Hang zhu Chun-Yang Meng Xiang-Ji Liu qing-san zhu Department of Spine Surgery China-Japan Union Hospital of Jilin UniversityChangchun 130033Jilin ProvinceChina

BACKGROUND As one of the most common complications of osteoporosis,osteoporotic vertebral compression fracture(OVCF)increases the risk of disability and mortality in elderly *** vertebroplasty(PVP)is considered to be an effective,safe,and minimally invasive treatment for *** recollapse of cemented vertebrae is one of the serious complications of ***,the risk factors associated with recollapse after PVP remain *** To identify risk factors for the recollapse of cemented vertebrae after PVP in patients with *** A systematic search in EMBASE,MEDLINE,the Cochrane Library,and PubMed was conducted for relevant studies from inception until March *** investigating risk factors for the recollapse of cemented vertebrae after PVP without additional trauma were selected for *** ratios(ORs)or standardized mean differences with 95%confidence interval(CI)were calculated and heterogeneity was assessed by both the chi-squared test and the I-squared *** methodological quality of the included studies was assessed according to the Newcastle-Ottawa *** A total of nine case-control studies were included in our meta-analysis comprising 300 cases and 2674 *** significant risk factors for the recollapse of cemented vertebrae after PVP in OVCF patients were fractures located at the thoracolumbar junction(OR=2.09;95%CI:1.30 to 3.38;P=0.002),preoperative intravertebral cleft(OR=2.97;95%CI:1.93 to 4.57;P<0.00001),and solid lump distribution pattern of the cement (OR = 3.11;95%CI: 1.91 to 5.07;P < 0.00001).The analysis did not support that age, gender, lumbar bone mineral density,preoperative visual analogue scale score, injected cement volume, intradiscalcement leakage, or vertebral height restoration could increase the risk forcemented vertebra recollapse after PVP in *** meta-analysis suggests that thoracolumbar junction fractures, preoperativeintravertebral cleft, and solid lump

关键词： Vertebroplasty Osteoporotic vertebral compression fracture Risk factors Recollapse Cemented vertebrae Meta-analysis

Thymosin Beta-4 Recombinant Adeno-associated Virus Enhances Human Nucleus Pulposus Cell Proliferation and Reduces Cell Apoptosis and Senescence

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Chinese Medical Journal 2015年第11期128卷 1529-1535页

作者： Yuan-Yi Wang qing-san zhu Yi-Wei Wang Ruo-Feng Yin Department of Spine Surgery The First Hospital of Jilin University Changchun Jilin 130021 China Department of OrthoPedics China-Japan UnionHospital of Jilin University C-hangchun Jilin 130021 China Department of Pathophysiology Jiangsu Jiankang Vocational College Nanjing Jiangsu 210029 China

Background： Thymosin beta-4 （TB-4） is considered key roles in tissue development, maintenance and pathological processes. The study aimed to prove TB-4 positive biological function on nucleus pulposus （NP） cell apoptosis and slowing the process of cell aging while increasing the cell proliferation. Methods： TB-4 recombinant adeno-associated virus （AAV） was constructed and induced to human NP cells. Cell of same group were cultured without gene modification as controlled group. Proliferation capacity and cell apoptosis were observed during 6 passages of the cells. Morphology and expression of the TB-4 gene were documented as parameter of cell activity during cell passage. Results： NP cells with TB-4 transfection has normal TB-4 expression and exocytosis. NP cells with TB-4 transfection performed significantly higher cell activity than that at the control group in each generation. TB-4 recombinant AAV-transfected human NP cells also show slower cell aging, lower cell apoptosis and higher cell proliferation than control group. Conclusions： TB-4 can prevent NP cell apoptosis, slow NP cell aging and promote NP cell proliferation. AAV transfection technique was able to highly and stably express TB-4 in human NP cells, which may provide a new pathway for innovation in the treatment of intervertebral disc degenerative diseases.

关键词： Biological Treatment Cell Aging Degeneration of Intervertebral Disc Thymosin Beta-4

Effect of pyrite content on chalcopyrite flotation under different regrinding conditions

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 49-57页

作者： Zejun Wang qing Shi Guofan Zhang Yuxuan zhu Binbin Li School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources Central South UniversityChangsha 410083China

This study aimed to investigate the effect of varying pyrite(Py)content on copper(Cu)in the presence of different regrinding conditions,which were altered using different types of grinding media:iron,ceramic balls,and their mixture,followed by flotation in the cleaner *** flotation performance of rough Cu concentrate can be improved by changing the regrinding conditions based on the Py *** electron microscopy,X-ray spectrometry,ethylenediaminetetraacetic acid disodium salt extraction,and X-ray photoelectron spectroscopy studies illustrated that when the Py content was high,the use of iron media in regrinding promoted the generation of hydrophilic Fe OOH on the surface of Py and improved the Cu *** ceramic medium with a low Py content prevented excessive Fe OOH from covering the surface of chalcopyrite(Cpy).Electrochemical studies further showed that the galvanic corrosion current of Cpy-Py increased with the addition of Py and became stronger with the participation of iron media.

关键词： chalcopyrite regrinding media pyrite galvanic corrosion flotation

Characteristic changes in astrocyte properties during astrocyte-to-neuron conversion induced by NeuroD1/Ascl1/Dlx2

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Neural Regeneration Research 2025年第6期20卷 1801-1815页

作者： qing He Zhen Wang Yuchen Wang Mengjie zhu Zhile Liang Kanghong Zhang Yuge Xu Gong Chen GHM Institute of CNS Regeneration Jinan UniversityGuangzhouGuangdong ProvinceChina

Direct in vivo conversion of astrocytes into functional new neurons induced by neural transcription factors has been recognized as a potential new therapeutic intervention for neural injury and degenerative disorders. However, a few recent studies have claimed that neural transcription factors cannot convert astrocytes into neurons, attributing the converted neurons to pre-existing neurons mis-expressing transgenes. In this study, we overexpressed three distinct neural transcription factors––NeuroD1, Ascl1, and Dlx2––in reactive astrocytes in mouse cortices subjected to stab injury, resulting in a series of significant changes in astrocyte properties. Initially, the three neural transcription factors were exclusively expressed in the nuclei of astrocytes. Over time, however, these astrocytes gradually adopted neuronal morphology, and the neural transcription factors was gradually observed in the nuclei of neuron-like cells instead of astrocytes. Furthermore,we noted that transcription factor-infected astrocytes showed a progressive decrease in the expression of astrocytic markers AQP4(astrocyte endfeet signal), CX43(gap junction signal), and S100β. Importantly, none of these changes could be attributed to transgene leakage into preexisting neurons. Therefore, our findings suggest that neural transcription factors such as NeuroD1, Ascl1, and Dlx2 can effectively convert reactive astrocytes into neurons in the adult mammalian brain.

关键词： aquaporin-4 Ascl1 astrocyte cortex Dlx2 gap junction glia-to-neuron conversion neural regeneration NeuroD1 reprogramming

A novel texture clustering method based on shift invariant DWT and locality preserving projection

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第2期10卷 247-252页

作者： Rui XING san-yuan ZHANG Le-qing zhu School of Computer Science and Technology Zhejiang University Hangzhou 310027 China

We propose a novel texture clustering method. A classical type of(approximate) shift invariant discrete wavelet transform(DWT),dual tree DWT,is used to decompose texture images. Multiple signatures are generated from the obtained high-frequency bands. A locality preserving approach is applied subsequently to project data from high-dimensional space to low-dimensional space. Shift invariant DWT can represent image texture information efficiently in combination with a histogram signature,and the local geometrical structure of the dataset is preserved well during clustering. Experimental results show that the proposed method remarkably outperforms traditional ones.

关键词： Shift invariant DWT. Texture signature Local preserving clustering Dimension reduction k-means