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Complement factor B knockdown by short hairpin RNA inhibits laser-induced choroidal neovascularization in rats

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International Journal of Ophthalmology(English edition) 2020年第3期13卷 382-389页

作者： Xin Wang qing-li shang Jing-Xue Ma Shu-Xia liu Cai-Xia Wang Cheng Ma Department of Ophthalmology the Second Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina Department of Pathology Hebei Medical UniversityShijiazhuang 050017Hebei ProvinceChina Department of Ophthalmology Tianjin First Central HospitalTianjin 300192China

AIM:To evaluate whether recombinant complement factor B(CFB)short hairpin RNA(sh RNA)reduces laserinduced choroidal neovascularization(CNV)in ***:Laser-induced rat CNV model was established,and then the animals underwent fundus fluorescence angiography(FFA)and hematoxylin and eosin(HE)*** day 3 and 7 after photocoagulation,the expression of CFB and membrane attack complex(MAC)was detected by immunhischemistry.A recombinant CFBsh RNA plasmid was *** and scrambled sh RNA plasmids were intravenous injected into rats via the tail vein on the day of laser treatment,*** day 7,the incidence of CNV was determined by FFA,and the expression of CFB and vascular endothelial growth factor(VEGF)in retinal pigment epithelium(RPE)/choroidal tissues was detected by immunhischemistry,Western blot and/or semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)in CFB and scrambled sh RNA *** possible adverse effects of CFB-sh RNA injection were assessed by transmission electron microscopy and ***:FFA and HE results indicated that a laserinduced rat CNV model was successfully established on day 7 after *** expression of CFB and MAC was extremely weak in normal retina and choroid,and increased on day 3 after ***,it started to reduce on day *** sh RNA plasmid was successfully constructed and induced CFB knockdown in the retinal and choroidal *** showed CFB knockdown significantly inhibited incidence of CNV in ***,CFB knockdown significantly inhibited the expression of VEGF in RPE/choroidal *** sh RNA caused no obvious side effects in ***:CFB knockdown significantly inhibits the formation and development of CNV in vivo through reducing the expression of VEGF,which is a potential therapy *** alternative pathway of complement activation plays an important role in CNV formation.

关键词： choroidal neovascularization complement factor B short hairpin RNA membrane attack complex vascular endothelial growth factor

A pilot study on vitrectomy combined with scleral shortening for eyes with myopic macular retinoschisis——2y follow-up results

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International Journal of Ophthalmology(English edition) 2021年第2期14卷 263-268页

作者： Peng Zhang Cun-Xi Ye ling-Xin Hou Yu-Hua Hao Kun Wang qing-li shang Department of Ophthalmology the Second Hospital of HebeiMedical UniversityShijiazhuang 050000Hebei ProvinceChina

AIM:To evaluate the effect of vitrectomy combined with scleral shortening for eyes with myopic macular ***:Thirty-seven patients with myopic macular retinoschisis who underwent pars plana vitrectomy(PPV)combined with scleral shortening were *** length(AL),the height of macular retinoschisis,the height of retinal detachment if existed,the diameter of macular hole if existed and best corrected visual acuity(BCVA)were *** preoperative and postoperative parameters were ***:At postoperative 24 mo,the mean AL and height of macular retinoschisis were reduced significantly by 0.79 mm and 256.51μm(t=8.064,P<0.0001;Z=-5.086,P<0.0001)*** addition,the mean height of retinal detachment and diameter of macular hole were also reduced significantly by 365.38μm and 183.68μm(Z=-4.457,P=0.000008;Z=-2.983,P=0.003)***,the postoperative BCVA was improved markedly(Z=-2.126,P=0.033).CONCLUSION:Vitrectomy combined with scleral shortening is an effective surgical method for eyes with myopic macular retinoschisis,whether or not macular hole and retinal detachment are present.

关键词： macula retinoschisis myopia scleral shortening vitrectomy

Relationship between the expression of Toll-like receptor 2 and 4 in mononuclear cells and postoperative acute lung injury in orthotopic liver transplantation

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Chinese Medical Journal 2009年第8期122卷 895-899页

作者： CHI Xin-jin CAI Jun LUO Chen-fang CHENG Nan HEI Zi-qing li shang-rong LUO Gang-jian Department of Anesthesiology Third Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong 510630 China

Background The aim of this study was to investigate the potential relationship between the dynamic expression of Toll-like receptor 2 and 4 （TLR2/4） in peripheral blood mononuclear cells as well as changes in serum concentration of inflammatory factors and acute lung injury （ALl） in patients after orthotopic liver transplantation （OLT）. Methods The peripheral blood samples of 27 patients （23 men and 4 women with ASA Ⅲ to Ⅳ） who received OLT were collected for measurement of TLR2/4 at T1 （after induction of anesthesia）, T2 （25 minutes after anhepatic phase）, T3 （3 hours after graft reperfusion） and T4 （24 hours after graft reperfusion）. The expression of TLR2/4 in mononuclear cells was measured by flow cytometry. The serum concentrations of tumor necrosis factor （TNF）-α, interleukin （IL）-1β and IL-8 were measured by enzyme-linked immunosorbent assay （EliSA）. Twenty-seven patients were assigned to ALl group （n=-9） and non-ALl group （n=-18） according to the diagnostic criteria of ALl. The expression of TLR2/4 in the ALl group or non-ALl group was analyzed. Results Compared to the non-ALl group, the volumes of blood loss, ascites, total output and transfused red blood cells were higher in the ALl group, and the anhepatic phase lasted longer （P 〈0.05, P 〈0.01）. The expression of TLR2/4 in mononuclear cells increased significantly at T3 and T4, and serum concentrations of TNF-α, IL-1β and IL-8 increased significantly too. There was no significant difference in Child-Turcotte-Pugh （CTP） scores between the ALl group and non-ALl group （P 〉0.05）. The expression of TLR2/4 in mononuclear cells increased significantly at T3 and T4 in the ALl group （P 〈0.05, P〈0.01）. A positive correlation was noted between the expression of TLR4 in mononuclear cells and the serum concentrations of TNF-α, IL-1β （P=0.041, P=0.046） in the ALl group. In the non-ALl group, statistical results showed that the expression level of TLR2/4 in mononuclear cells was not significantly different during the pe

关键词： orthotopic liver transplantation Toll-like receptor 2 Toll-like receptor 4 acute lung injury

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新立城水库藻类污染成因分析及治理对策措施

水文 2008年第6期28卷 45-46,14页

作者：李青山苏保健松辽流域水资源保护局吉林长春130021 吉林省水文水资源局吉林长春130022

本文对新立城水库发生蓝藻水华事件进行了水质应急监测和污染成因分析。从新立城水库气象条件分析,发现水库水温是历史同期最高值,为藻类在库区内大量迅速繁殖提供了温度条件;从新立城水库坝前、库中和库末氮磷浓度分析,发现水库氮磷浓... 详细信息

本文对新立城水库发生蓝藻水华事件进行了水质应急监测和污染成因分析。从新立城水库气象条件分析,发现水库水温是历史同期最高值,为藻类在库区内大量迅速繁殖提供了温度条件;从新立城水库坝前、库中和库末氮磷浓度分析,发现水库氮磷浓度均超过富营养化临界值,为藻类在库区内大量迅速繁殖提供了营养条件。本文在分析水库藻类污染成因基础上,提出了从水库管理和现有水处理系统的改进入手,加强新立城水库外源污染控制对策措施和内源污染治理措施。

关键词：藻类污染成因分析治理措施新立城水库

Effects of carbon content on the microstructure and tensile properties of a low-density steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 391-401页

作者： Yongxuan shang Mingyu Fan Shuyong Jiang Zhongwu Zhang Key Laboratory of Superlight Materials and Surface Technology Ministry of EducationCollege of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001China State Key Laboratory of Metal Material for Marine Equipment and Application Iron&Steel Research Institute of Ansteel Group CorporationAnshan 114009China

Carbon can change the phase components of low-density steels and influence the mechanical *** this study,a new method to control the carbon content and avoid the formation ofδ-ferrite by decarburization treatment was *** microstructural changes and mechanical characteristics with carbon content induced by decarburization were systematically ***-Jaoul(C-J)analysis was employed to examine the work hardening characteristics during the tensile *** decarburization by heat treatments,the carbon content within the austenite phase decreased,while Mn and Al were almost unchanged;this made the steel with full austenite transform into the austenite and ferrite dual ***,(Ti,V)C carbides existed in both matrix phase and the mole fraction almost the *** addition,the formation of other carbides *** loss induced a decrease in strength due to the weakening of the carbon solid *** the steel with the single austinite,the deformation mode of austenite was the dislocation planar glide,resulting in the formation of *** the dual-phase steel,the deformation occurred by the dislocation planar glide of austenite first,with the increase in strain,the cross slip of ferrite took place,forming dislocation cells in *** the late stage of deformation,the work hardening of austinite increased rapidly,while that of ferrite increased slightly.

关键词： low-density steels carbon content decarburization strengthening mechanisms work hardening behavior

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氧等离子环境下影响多孔硅光学特性的因素

激光与红外 2011年第7期41卷 788-792页

作者：李平李清山张世玉马自侠鲁东大学物理学院山东烟台264025

研究了多孔硅(porous silicon,PS)在氧等离子体环境中退火温度和存储时间对PS光学稳定性的影响。通过光致发光(PL)谱和傅里叶变换红外光谱对系列样品进行分析。高斯拟合结果显示PL谱由三个高斯峰叠加而成,其中至少两个高斯峰是由非量子... 详细信息

研究了多孔硅(porous silicon,PS)在氧等离子体环境中退火温度和存储时间对PS光学稳定性的影响。通过光致发光(PL)谱和傅里叶变换红外光谱对系列样品进行分析。高斯拟合结果显示PL谱由三个高斯峰叠加而成,其中至少两个高斯峰是由非量子限制效应造成的,即第一峰面积的变化与Si=O双键密切相关,第三峰面积的变化与Si-O-Si桥键以及SiHx(x=1,2)键有关;退火温度对以上两个峰的强弱变化有直接影响。

关键词：多孔硅快速退火氧化非量子限制效应

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单位球上F(p,q,s)空间的分解和刻画

数学学报（中文版） 2018年第6期61卷 1037-1048页

作者：肖建斌张学军尚清丽郭雨婷杭州电子科技大学杭州310037 湖南师范大学数学与统计学院长沙410006

设p〉0，s ≥ 0，q〉max{-n-1，-s-1}，本文探讨了单位球上F（p，q，s）空间的一种等价刻画和分解问题.具体结果为：（1） f∈ F（p，q，s）当且仅当f∈ H（B），且I^p=supa∈B∫B｜R^α，γf（z）｜^p（1-｜z｜2）q＋pγ-p（1-｜φa（... 详细信息

设p〉0，s ≥ 0，q〉max{-n-1，-s-1}，本文探讨了单位球上F（p，q，s）空间的一种等价刻画和分解问题.具体结果为：（1） f∈ F（p，q，s）当且仅当f∈ H（B），且I^p=supa∈B∫B｜R^α，γf（z）｜^p（1-｜z｜2）q＋pγ-p（1-｜φa（z）｜^2）^s dv（z）〈∞，其中α〉-1 和γ〉max{0，1-（q＋s＋1）/p，1-（q＋n＋1）/p}. （2）若{dk}∈ ∫p，则存在序列{wk}？B，使得 f（z）=∑k=1^∞（dk（1-｜wk｜2）t＋1）/（1-〈z，wk〉）t＋（q＋n＋1）/p）（z∈B）属于F（p，q，s），其中t〉max{1-1/p，0}（q＋n＋1）＋max{1/p，1}s-1.

关键词： F(p,q,s)空间等价刻画原子分解单位球

Unraveling the significance of cobalt on transformation kinetics,crystallography and impact toughness in high-strength steels

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 380-390页

作者： Yishuang Yu Jingxiao Zhao Xuelin Wang Hui Guo Zhenjia Xie Chengjia shang Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China

This work reveals the significant effects of cobalt(Co)on the microstructure and impact toughness of as-quenched highstrength steels by experimental characterizations and thermo-kinetic *** results show that the Co-bearing steel exhibits finer blocks and a lower ductile-brittle transition temperature than the steel without ***,the Co-bearing steel reveals higher transformation rates at the intermediate stage with bainite volume fraction ranging from around 0.1 to *** improved impact toughness of the Co-bearing steel results from the higher dense block boundaries dominated by the V1/V2 variant ***,the addition of Co induces a larger transformation driving force and a lower bainite start temperature(BS),thereby contributing to the refinement of blocks and the increase of the V1/V2 variant *** findings would be instructive for the composition,microstructure design,and property optimization of high-strength steels.

关键词： high-strength steel cobalt transformation kinetics crystallography impact toughness

莪术醇抑制VEGF诱导的新生血管生成的实验研究

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国际眼科杂志 2023年第3期23卷 379-384页

作者：郝雨檬王彩霞马景学李雪景尚庆丽河北医科大学第二医院眼科河北省石家庄市050000 中国人民解放军联勤保障部队第九八〇医院眼科河北省石家庄市050051

目的:研究莪术醇对血管内皮生长因子(VEGF)诱导的新生血管生成的作用及机制。方法:体外培养人脐静脉血管内皮细胞,用50ng/mL VEGF和不同浓度莪术醇进行分组处理,采用CCK-8和EdU实验检测细胞增殖,Transwell实验分析细胞迁移能力,管腔形... 详细信息

目的:研究莪术醇对血管内皮生长因子(VEGF)诱导的新生血管生成的作用及机制。方法:体外培养人脐静脉血管内皮细胞,用50ng/mL VEGF和不同浓度莪术醇进行分组处理,采用CCK-8和EdU实验检测细胞增殖,Transwell实验分析细胞迁移能力,管腔形成实验分析内皮细胞血管生成能力,Western blot检测Akt/mTORC1通路变化。结果:CCK-8实验结果显示,400、800μmol/L莪术醇+VEGF组细胞OD450值明显低于VEGF组(均P<0.01)。EdU结果显示,400μmol/L莪术醇+VEGF组细胞增殖率明显低于VEGF组(P<0.001)。Transwell实验和管腔形成实验结果显示,与VEGF组比较,400μmol/L莪术醇+VEGF组迁移细胞数减少,管腔形成的分支数量和分支长度下降(均P<0.001)。Western blot结果显示,莪术醇可显著减少细胞中Akt/mTORC1下游靶点p-Akt和p-S6的表达。结论:莪术醇可抑制VEGF诱导的血管内皮细胞的增殖、迁移和管腔形成,具有强的抑制血管生成的作用,可进一步用于眼底新生血管的治疗研究。

关键词：莪术醇新生血管生成血管内皮生长因子(VEGF) Akt/mTORC1通路