Complement factor B knockdown by short hairpin RNA inhibits laser-induced choroidal neovascularization in rats

作     者：Xin Wang Qing-Li Shang Jing-Xue Ma Shu-Xia Liu Cai-Xia Wang Cheng Ma 

作者机构：Department of Ophthalmologythe Second Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina Department of PathologyHebei Medical UniversityShijiazhuang 050017Hebei ProvinceChina Department of OphthalmologyTianjin First Central HospitalTianjin 300192China 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2020年第13卷第3期

页      面：382-389页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

主　　题：choroidal neovascularization complement factor B short hairpin RNA membrane attack complex vascular endothelial growth factor 

摘      要：AIM:To evaluate whether recombinant complement factor B(CFB)short hairpin RNA(sh RNA)reduces laserinduced choroidal neovascularization(CNV)in ***:Laser-induced rat CNV model was established,and then the animals underwent fundus fluorescence angiography(FFA)and hematoxylin and eosin(HE)*** day 3 and 7 after photocoagulation,the expression of CFB and membrane attack complex(MAC)was detected by immunhischemistry.A recombinant CFBsh RNA plasmid was *** and scrambled sh RNA plasmids were intravenous injected into rats via the tail vein on the day of laser treatment,*** day 7,the incidence of CNV was determined by FFA,and the expression of CFB and vascular endothelial growth factor(VEGF)in retinal pigment epithelium(RPE)/choroidal tissues was detected by immunhischemistry,Western blot and/or semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)in CFB and scrambled sh RNA *** possible adverse effects of CFB-sh RNA injection were assessed by transmission electron microscopy and ***:FFA and HE results indicated that a laserinduced rat CNV model was successfully established on day 7 after *** expression of CFB and MAC was extremely weak in normal retina and choroid,and increased on day 3 after ***,it started to reduce on day *** sh RNA plasmid was successfully constructed and induced CFB knockdown in the retinal and choroidal *** showed CFB knockdown significantly inhibited incidence of CNV in ***,CFB knockdown significantly inhibited the expression of VEGF in RPE/choroidal *** sh RNA caused no obvious side effects in ***:CFB knockdown significantly inhibits the formation and development of CNV in vivo through reducing the expression of VEGF,which is a potential therapy *** alternative pathway of complement activation plays an important role in CNV formation.