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Antihepatoma peptide,scolopentide,derived from the centipede scolopendra subspinipes mutilans

World Journal of Gastroenterology 2023年第12期29卷 1875-1898页

作者： Yu-Xing Hu Zhuo Liu Zhen Zhang Zhe Deng Zhen Huang Ting Feng qing-hong zhou Si Mei Chun Yi qing zhou Pu-Hua Zeng Gang Pei Sha Tian Xue-Fei Tian College of Integrated Chinese and Western Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Scientific Research Hunan Academy of Traditional Chinese Medicine Affiliated HospitalChangsha 410208Hunan ProvinceChina Hunan Province University Key Laboratory of Oncology of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Pediatric Shenzhen Hospital of Beijing University of Chinese MedicineShenzhen 518000Guangdong ProvinceChina Department of Physiology Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Pathology Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Andrology First Hospital of Hunan University of Chinese MedicineChangsha 410007Hunan ProvinceChina Department of Oncology Hunan Academy of Traditional Chinese Medicine Affiliated HospitalChangsha 410208Hunan ProvinceChina College of Pharmacy Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Dr Neher’s Biophysics Laboratory for Innovative Drug Discovery Macao University of Science and TechnologyMacao 999078China

BACKGROUND Centipedes have been used to treat tumors for hundreds of years in ***,current studies focus on antimicrobial and anticoagulation agents rather than *** molecular identities of antihepatoma bioactive components in centipedes have not yet been extensively *** is a challenge to isolate and characterize the effective components of centipedes due to limited peptide purification technologies for animal-derived *** To purify,characterize,and synthesize the bioactive components with the strongest antihepatoma activity from centipedes and determine the antihepatoma *** An antihepatoma peptide(scolopentide)was isolated and identified from the centipede scolopendra subspinipes mutilans using a combination of enzymatic hydrolysis,a Sephadex G-25 column,and two steps of high-performance liquid chromatography(HPLC).Additionally,the CCK8 assay was used to select the extracted fraction with the strongest antihepatoma *** molecular weight of the extracted scolopentide was characterized by quadrupole time of flight mass spectrometry(QTOF MS),and the sequence was matched by using the Mascot search *** on the sequence and molecular weight,scolopentide was synthesized using solid-phase peptide synthesis *** synthetic scolopentide was confirmed by MS and *** antineoplastic effect of extracted scolopentide was confirmed by CCK8 assay and morphological changes again in *** antihepatoma effect of synthetic scolopentide was assessed by the CCK8 assay and Hoechst staining in vitro and tumor volume and tumor weight in *** the tumor xenograft experiments,qualified model mice(male 5-week-old BALB/c nude mice)were randomly divided into 2 groups(n=6):The scolopentide group(0.15 mL/d,via intraperitoneal injection of synthetic scolopentide,500 mg/kg/d)and the vehicle group(0.15 mL/d,via intraperitoneal injection of normal saline).The mice were euthanized by cervical dislocation after 14 d of con

关键词： Scolopendra Centipede Antihepatom peptide Hepatocellular carcinoma Death receptor 4 Death receptor 5

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Clinical significance of EPHX2 deregulation in prostate cancer

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Asian Journal of Andrology 2021年第1期23卷 109-115页

作者： Ming-Sheng Liu Hui Zhao Chen-Xiang Xu Ping-Bo Xie Wei Wang Ying-Yu Yang Wen-Hui Lee Yang Jin hong-qing zhou The Second Ward of Urology Qujing Affiliated Hospital of Kunming Medical UniversityQujing 655000China Department of Urology The First Affiliated Hospital of Kunming Medical UniversityKunming 650332China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Institute for Cancer Genetics and Informatics Oslo University HospitalOslo 0379Norway

The arachidonic acid(AA)metabolic pathway participates in various physiological processes as well as in the development of *** analyzed genomic alterations in AA metabolic enzymes in the Cancer Genome Atlas(TCGA)prostate cancer(PCa)dataset and found that the gene encoding soluble epoxide hydrolase(EPHX2)is frequently deleted in ***2 mRNA and protein expression in PCa was examined in multiple datasets by differential gene expression analysis and in a tissue microarray by *** expression data were analyzed in conjunction with clinicopathological *** the mRNA and protein expression levels of EPHX2 were significantly decreased in tumors compared with normal prostate tissues and were inversely correlated with the Gleason grade and disease-free survival ***,EPHX2 mRNA expression was significantly decreased in metastatic and recurrent PCa compared with localized and primary PCa,*** addition,EPHX2 protein expression correlated negatively with Ki67 *** conclusion,EPHX2 deregulation is significantly correlated with the clinical characteristics of PCa progression and may serve as a prognostic marker for PCa.

关键词： arachidonic acid metabolism metastasis prognosis prostate cancer soluble epoxide hydrolase

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Tailoring Light–Matter Interactions in Overcoupled Resonator for Biomolecule Recognition and Detection

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Nano-Micro Letters 2025年第1期17卷 262-280页

作者： Dongxiao Li hong zhou Zhihao Ren Cheng Xu Chengkuo Lee Department of Electrical and Computer Engineering National University of SingaporeSingapore 117583Singapore Center for Intelligent Sensors and MEMS(CISM) National University of SingaporeSingapore 117608Singapore

Plasmonic nanoantennas provide unique opportunities for precise control of light–matter coupling in surface-enhanced infrared absorption(SEIRA)spectroscopy,but most of the resonant systems realized so far suffer from the obstacles of low sensitivity,narrow bandwidth,and asymmetric Fano resonance ***,we demonstrated an overcoupled resonator with a high plasmon-molecule coupling coefficient(μ)(OC-Hμresonator)by precisely controlling the radiation loss channel,the resonator-oscillator coupling channel,and the frequency detuning *** observed a strong dependence of the sensing performance on the coupling state,and demonstrated that OC-Hμresonator has excellent sensing properties of ultra-sensitive(7.25%nm^(−1)),ultra-broadband(3–10μm),and immune asymmetric Fano *** characteristics represent a breakthrough in SEIRA technology and lay the foundation for specific recognition of biomolecules,trace detection,and protein secondary structure analysis using a single array(array size is 100×100μm^(2)).In addition,with the assistance of machine learning,mixture classification,concentration prediction and spectral reconstruction were achieved with the highest accuracy of 100%.Finally,we demonstrated the potential of OC-Hμresonator for SARS-CoV-2 *** findings will promote the wider application of SEIRA technology,while providing new ideas for other enhanced spectroscopy technologies,quantum photonics and studying light–matter interactions.

关键词： Plasmonic nanoantennas Light-matter interaction Surface-enhanced infrared absorption Overcoupled Biosensing

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北黄海冷水团对小型底栖动物时空分布的影响

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中国海洋大学学报（自然科学版） 2016年第12期46卷 60-67页

作者：于青周红张凯米雪张志南中国海洋大学海洋生命学院山东青岛266003

2011—2014年春夏季和秋冬季于北黄海冷水团及其周边海域进行采样,对小型底栖动物丰度、生物量、类群组成和群落结构的时空分布特征及其与环境因子的关系进行了研究。结合908专项调查,以多年数据分析探究冷水团对小型底栖动物的生态效... 详细信息

2011—2014年春夏季和秋冬季于北黄海冷水团及其周边海域进行采样,对小型底栖动物丰度、生物量、类群组成和群落结构的时空分布特征及其与环境因子的关系进行了研究。结合908专项调查,以多年数据分析探究冷水团对小型底栖动物的生态效应。调查海域共鉴定出小型底栖动物20个类群,总丰度中线虫占85%,桡足类占11%,其它类群仅占4%。小型底栖动物在春夏季和秋冬季的平均丰度分别为（961±797）、（679±553）ind/10cm2,平均生物量分别为（1 007±753）、（658±401）μg dwt/10cm2。三因素方差分析（three-way ANOVA）和二因素相似性分析（two-way ANOSIM）结果表明小型底栖动物丰度、生物量和群落结构在冷水团中心及其周边以及在春夏季和秋冬季之间皆有显著差异。群落结构的差异主要由桡足类、动吻类和介形类引起。Pearson相关分析表明丰度和生物量与底温呈显著负相关,与底盐呈显著正相关。小型底栖动物丰度、生物量与有机碳水平分布规律相反。93%的小型底栖动物分布在沉积物0~5cm层,桡足类在0~2cm层分布率高于小型底栖动物平均水平。

关键词：小型底栖动物丰度生物量北黄海冷水团

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载柔红霉素膜微粒的制备及其对白血病细胞的增殖抑制作用

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中国药学杂志 2018年第7期53卷 526-530页

作者：向虹霖王慧洁卿红周瑶毛声俊李慧四川大学华西药学院靶向药物及释药系统教育部重点实验室成都610041 四川省医学科学院四川省人民医院血液科成都610072

目的构建载柔红霉素膜微粒给药系统,考察其对白血病细胞的增殖抑制作用。方法采用胞内与胞外载药2种方式,制备来源于HL-60细胞的载柔红霉素膜微粒,并表征其粒径、Zeta电位和载药量等特性。采用激光共聚焦显微镜与流式细胞术检测细胞对... 详细信息

目的构建载柔红霉素膜微粒给药系统,考察其对白血病细胞的增殖抑制作用。方法采用胞内与胞外载药2种方式,制备来源于HL-60细胞的载柔红霉素膜微粒,并表征其粒径、Zeta电位和载药量等特性。采用激光共聚焦显微镜与流式细胞术检测细胞对载药膜微粒的摄取,MTT法检测载药膜微粒对肿瘤细胞的增殖抑制作用。结果 2种方式所制得膜微粒的粒径和Zeta电位无明显差异,而胞外载药膜微粒的载药量较高。细胞摄取试验结果显示,载药膜微粒可显著增加细胞摄取(P<0.05),且胞内载药较胞外载药方式更优(P<0.05)。MTT试验结果表明,与游离药物相比,载药膜微粒可增强其对HL-60细胞的增殖抑制作用(P<0.05)。结论不同载药方式对膜微粒的抗肿瘤药效具有一定影响,膜微粒可显著增强细胞摄取与药效,有望成为具有潜力的治疗白血病的新型递药载体。

关键词：膜微粒白血病柔红霉素

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AZD1775 synergizes with SLC7A11 inhibition to promote ferroptosis

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Science China Life Sciences 2025年第1期 204-218页

作者： Chen Xiong hong Ling Yingdan Huang Hanzhi Dong Bangxiang Xie Qian Hao Xiang zhou Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences Fudan University Department of Oncology Shanghai Medical CollegeFudan University Department of Breast Surgery Fudan University Shanghai Cancer CenterFudan University Department of Oncology First Affiliated Hospital of Nanchang University Beijing Institute of Hepatology Beijing Youan HospitalCapital Medical University Beijing Engineering Research Center for Precision Medicine and Transformation of Hepatitis and Liver Cancer Key Laboratory of Breast Cancer in Shanghai Fudan University Shanghai Cancer CenterFudan University Shanghai Key Laboratory of Medical Epigenetics International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology)Institutes of Biomedical SciencesFudan University

Tumor suppressor p53-mediated cell cycle arrest and DNA damage repair may exert cytoprotective effects against cancer therapies, including WEE1 inhibition. Considering that p53 activation can also lead to multiple types of cell death, the role of this tumor suppressor in WEE1 inhibitor-based therapies remains disputed. In this study, we reported that nucleolar stress-mediated p53 activation enhanced the WEE1 inhibitor AZD1775-induced ferroptosis to suppress lung cancer growth. Our findings showed that AZD1775 promoted ferroptosis by blocking cystine uptake, an action similar to that of Erastin. Meanwhile, inhibition of WEE1 by the WEE1 inhibitors or si RNAs induced compensatory upregulation of SLC7A11, which conferred resistance to ferroptosis. Mechanistically, AZD1775 prevented the enrichment of H3K9me3, a histone marker of transcriptional repression, on the SLC7A11 promoter by repressing the expression of the histone methyltransferase SETDB1, thereby enhancing NRF2-mediated SLC7A11 transcription. This finding was also validated using the H3K9me3 inhibitor BRD4770. Remarkably, we found that the nucleolar stress-inducing agent Actinomycin D(Act. D) inhibited SLC7A11 expression by activating p53, thus augmenting AZD1775-induced ferroptosis. Moreover, the combination of AZD1775 and Act. D synergistically suppressed wild-type p53-harboring lung cancer cell growth both in vitro and in vivo. Altogether, our study demonstrates that AZD1775 promotes ferroptosis by targeting cystine uptake but also mediates the adaptive activation of SLC7A11 through the WEE1-SETDB1 cascade and NRF2-induced transcription, and inhibition of SLC7A11 by Act. D boosts the anti-tumor efficacy of AZD1775 by enhancing ferroptosis in cancers with wild-type p53.

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RNA recognition motif (RRM) RNA-binding proteins from Bombyx mori

RNA recognition motif (RRM) RNA-binding proteins from Bombyx...

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华东六省一市生物化学与分子生物学学会2006年学术交流会

作者： Lin-Ling Wang,Ke-Ping Chen,Gui-Tian Gao,Zhi-Gang Hu, Hui-qing Chen,hong-Liang zhou,Qin Yao (Institute of Life Sciences,Jiangsu University, Jiangsu 212013,China)

RNA-binding proteins are important in many aspects of RNA processing, function, and destruction. One class of such proteins contains the RNA recognition motif (RRM), which consists of about 80 amino acid residues. 134 protein sequences of known Drosophila melanogaster RRM-containing proteins were used to query the nucleotide sequence of the B. mori genome and EST database, with a result that 123 protein sequences have obvious orthologues in the Bombyx mori genome and 89 different RRM-containing proteins were found to have obvious orthologues in EST database of B. mori. We analysed the transcription factor binding sites of 41 RRM-containing protein genes in the silkworm genome and gave a comparative analysis of their upstream regions. We performed multiple sequence alignments and plotted a cladogram of the 23 new RRM proteins having complete cds in the hope of discerning functional or evolutionary relationships. Functions of RRM proteins from silkworm are predicted based on the similarity with their Drosophila, yeast and human counterparts. This survey provides the first systematic analysis of silkworm RRM-containing proteins, which may serve as a basis for functional characterization of this important protein group in silkworm.

关键词： RRM-containing protein Bombyx mori Transcription factor binding site ALY Tsunag

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Overexpression of low-density lipoprotein receptor prevents neurotoxic polarization of astrocytes via inhibiting NLRP3 inflammasome activation in experimental ischemic stroke

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Neural Regeneration Research 2025年第2期20卷 491-502页

作者： Shuai Feng Juanji Li Tingting Liu Shiqi Huang Xiangliang Chen Shen Liu Junshan zhou hongdong Zhao Ye hong Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Jinling HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina

Neurotoxic astrocytes are a promising therapeutic target for the attenuation of cerebral ischemia/reperfusion ***-density lipoprotein receptor,a classic cholesterol regulatory receptor,has been found to inhibit NLR family pyrin domain containing protein 3(NLRP3)inflammasome activation in neurons following ischemic stroke and to suppress the activation of microglia and astrocytes in individuals with Alzheimer’s ***,little is known about the effects of low-density lipoprotein receptor on astrocytic activation in ischemic *** address this issue in the present study,we examined the mechanisms by which low-density lipoprotein receptor regulates astrocytic polarization in ischemic stroke ***,we examined low-density lipoprotein receptor expression in astrocytes via immunofluorescence staining and western blotting *** observed significant downregulation of low-density lipoprotein receptor following middle cerebral artery occlusion reperfusion and oxygen-glucose deprivation/***,we induced the astrocyte-specific overexpression of low-density lipoprotein receptor using astrocyte-specific adeno-associated ***-density lipoprotein receptor overexpression in astrocytes improved neurological outcomes in middle cerebral artery occlusion mice and reversed neurotoxic astrocytes to create a neuroprotective ***,we found that the overexpression of low-density lipoprotein receptor inhibited NLRP3 inflammasome activation in oxygen-glucose deprivation/reoxygenation injured astrocytes and that the addition of nigericin,an NLRP3 agonist,restored the neurotoxic astrocyte *** findings suggest that low-density lipoprotein receptor could inhibit the NLRP3-meidiated neurotoxic polarization of astrocytes and that increasing low-density lipoprotein receptor in astrocytes might represent a novel strategy for treating cerebral ischemic stroke.

关键词： inflammation ischemia/reperfusion injury ischemic stroke low-density lipoprotein receptor neuroprotective astrocytes neurotoxic astrocytes NLRP3 inflammasome polarization

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Comparison of Two Approaches of Laparoscopic Varicocelectomy for the Treatment of Varicocele

Comparison of Two Approaches of Laparoscopic Varicocelectomy...

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第十三届全国泌尿外科学术会议暨第八届全球华人泌尿外科学术会议

作者： zhou Shui-gen, ZHANG Zheng-yu, GAO Jian-ping, GE Jing-ping, MA hong-qing, zhou Wen-quan, WEI Wu, CHENG Wen (Department of Urology, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, P.R. China)

Objective Varicocele is a highly prevalent medical problem in young men. Traditionally, open varicocelectomy at different levels (high or low ligation) is the most widely used treatment for the patients with varicocel... 详细信息

关键词： Varicocelectomy Laparoscopy

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Invasive fungal infections among people with acquired immune deficiency syndrome:clinical features and management

Invasive fungal infections among people with acquired immune...

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中华中医药学会防治艾滋病学术研讨会暨2006年年会

作者： Yin Zhong Shen~a,Tang Kai Qi~a,Jian Xin Ma~a,Xue Yan Jiang~a,Jiang Rong Wang~a, qing Nian Xu~a,Qin Huang~a,Xi Nian Liu~a,hong qing Sun~a,hong zhou Lu~(a,b,*) a Department of Infectious Diseases,Shanghai Public Health Center,Fudan University,Shanghai 201508,China b Department of Infectious Diseases,Huashan Hospital,Fudan University,Shanghai 200040,China

Objectives:This study aimed to analyze the clinical features,diagnosis and treatment of invasive fungal infections in acquired immune deficiency syndrome(AIDS)patients. Methods:We retrospectively analyzed 85 AIDS cases to summarize the clinical characteristics, epidemiology,diagnosis and treatment of invasive fungal infections in AIDS patients. Results:Of 85 AIDS cases,35 had complicated with invasive fungal infections;44.4%of them occurred in digestive tract;the major fungi isolated from patients were Candida albicans(57.4%);71.8 %of the invasive fungal infections occurred in patients whose CD4T lymphocyte counts were<100/μl.27 cases of them were cured and 8 died. Conclusions:Invasive fungal infection is one of the major opportunistic infections in AIDS patients;the occurrence of invasive fungal infection is associated with patients’ CD4T lymphocyte counts;most invasive fungal infections occur in digestive tract;the mortality rate is still high although many new antifungal drugs are available.

关键词： AIDS Fungal infection Epidemiology Diagnosis Treatment

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