检索结果-南通市图书馆

Assessment of the risk of infectious aerosols leaking to the environment from BSL-3 laboratory HEPA air filtration systems using model bacterial aerosols

在线全文

学校读者我要写书评

暂无评论

Particuology 2014年第2期12卷 82-87页

作者： Zhanbo Wen Wenhui Yang Na li Jie Wang lingfei Hu Jinsong li Zhe Yin Ke Zhang Xiaokai dong State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology

To assess the risk of infectious bacterial aerosols leaking to the environment, the filtration efficiency of a biosafety level 3 （BSL-3） laboratory high-efficiency particulate （HEPA） filter was investigated using the aerosolized bacteria Serratia marcescens. The aerosol size was measured using an Andersen sampler. Eight first stage HEPA filters （numbered 1-87 were distributed in contaminated labs and exhausts from each of the first stage HEPA filters were aggregated and filtered through one second stage HEPA filter before being released to the environment. In total, 8 first-stage and I second-stage HEPA filters from the BSL-3 air purification system were analyzed. No S. marcescens was detected in first stage filters 1,2, 4, 5, 7 and 8 and the second stage HEPA filter. The filtration efficiencies against aerosolized S. marcescens were 〉99.9999%. First stage filter numbers 3 and 6 had filtration efficiencies of 99.9825% and 99.9906% respectively. When filter number 3 was replaced by a new filter and the bracket for filter number 6 was sealed, no aerosolized S. marcescens was detected in the filtered air. Our work suggests that the BSL-3 laboratory HEPA filter air purification system is effective against bacterial aerosols, with little to no bacterial leakage into the environment.

关键词： Bacterial aerosolFiltration efficiencyHEPA filtersSerratia marcescens

Early detection of circulating tumor DNA and successful treatment with osimertinib in thr790met-positive leptomeningeal metastatic lung cancer:A case report

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第22期10卷 7968-7972页

作者： li-qing Xu Ying-Jin Wang Sheng-li Shen Yao Wu Hong-Zhou Duan Department of Neurosurgery Peking University First HospitalBeijing 100034China

BACKGROUND Patients diagnosed with non-small-cell lung cancer with activated epidermal growth factor receptor mutations are more likely to develop leptomeningeal(LM)metastasis than other types of lung cancers and have a poor *** diagnosis and effective treatment of leptomeningeal carcinoma can improve the *** SUMMARY A 55-year-old female with a progressive headache and vomiting for one month was admitted to Peking University First *** was diagnosed with lung adenocarcinoma with osseous metastasis 10 months prior to *** growth factor receptor(EGFR)mutation was detected by genomic examination,so she was first treated with gefitinib for 10 months before acquiring ***-free cerebrospinal fluid(CSF)circulating tumor DNA detection by next-generation sequencing was conducted and indicated the EGFR-Thr790Met mutation,while biopsy and cytology from the patient’s CSF and the first enhanced cranial magnetic resonance imaging(MRI)showed no positive findings.A month later,the enhanced MRI showed linear leptomeningeal enhancement,and the cytology and biochemical examination in CSF remained ***,osimertinib(80 mg/d)was initiated as a second-line treatment,resulting in a good response within a *** This report suggests clinical benefit of osimertinib in LM patients with positive detection of the EGFR-Thr790Met mutation in CSF and proposes that the positive findings of CSF circulating tumor DNA as a liquid biopsy technology based on the detection of cancer-associated gene mutations may appear earlier than the imaging and CSF findings and may thus be helpful for ***,the routine screening of chest CT with the novel coronavirus may provide unexpected benefits。

关键词： Non-small cell lung cancer Epidermal growth factor receptor mutation Circulating tumor DNA detection Leptomeningeal carcinomatosis Osimertinib Case report

Effects of discharge parameters on the micro-hollow cathode sustained glow discharge

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plasma Science and Technology 2018年第5期20卷 36-44页

作者： Shoujie HE Peng WANG Jing HA Baoming ZHANG Zhao ZHANG qing li College of physics science and technology Hebei UniversityBaoding 071002People＇s Republic of China Institute of Science Hebei Agriculture UniversityBaoding 071001People＇s Republic of China

The effects of parameters such as pressure,first anode radius,and the cavity diameter on the micro-hollow cathode sustained glow discharge are investigated by using a two-dimensional self-consistent fluid model in pure *** results indicate that the three parameters influence the discharge in the regions inside and outside of the *** a fixed voltage on each electrode,a larger volume of high density plasma can be produced in the region between the first and the second anodes by selecting the appropriate pressure,the higher first anode,and the appropriate cavity *** the pressure increases,the electron density inside the hollow cathode,the high density plasma volume between the first anode and second anodes,and the radial electric field in the cathode cavity initially increase and subsequently *** the cavity diameter increases,the high-density plasma volume between the first and second anodes initially increases and subsequently decreases;whereas the electron density inside the hollow cathode *** the first anode radius increases,the electron density increases both inside and outside of the ***,the increase of the electron density is more obvious in the microcathode sustained region than in the micro cavity *** results reveal that the discharge inside the cavity interacts with that outside the *** strong hollow cathode effect and the high-density plasma inside the cavity favor the formation of a sustained discharge between the first anode and the second *** also show that the radial boundary conditions exert a considerably weaker influence on the discharge except for a little change in the region close to the radial boundary.

关键词： micro hollow cathode sustained glow discharge (MCSD) fluid model dischargeparameters

在线全文

学校读者我要写书评

暂无评论

基于SRP-6协议的认证与密钥交换方案

华南理工大学学报（自然科学版） 2007年第1期35卷 94-98页

作者：李冬郭荷清韩涛华南理工大学计算机科学与工程学院广东广州510640

针对Web服务中身份认证的问题,分析了SRP-6(Secure Remote Password-6)协议的密钥交换机制,提出了一种基于简单对象访问协议(SOAP)和SRP-6协议的认证与密钥交换方案(SRP-over-SOAP).该方案将SOAP消息进行扩展,通过在SOAP头中加入标记,... 详细信息

针对Web服务中身份认证的问题,分析了SRP-6(Secure Remote Password-6)协议的密钥交换机制,提出了一种基于简单对象访问协议(SOAP)和SRP-6协议的认证与密钥交换方案(SRP-over-SOAP).该方案将SOAP消息进行扩展,通过在SOAP头中加入标记,实现了SOAP消息对SRP认证信息的传递.文中还将该方案用于Web服务,实现了服务器和客户机间的双向身份认证.

关键词： SRP协议简单对象访问协议认让密钥交换 Web服务

Influence of deflection angles on flow behaviours in openchannel bends

在线全文

学校读者我要写书评

暂无评论

Journal of Mountain Science 2018年第10期15卷 2292-2306页

作者： li Bing-dong ZHANG Xin-hua TANG Han-song TSUBAKI Ryota State Key Laboratory of Hydraulics and Mountain River Engineering Sichuan University Department of Civil Engineering/City College City University of New York Department of Civil Engineering Nagoya University

The deflection angle of a river bend plays an important role on behaviours of the flow within it, and a clear understanding of the angle's influence is significant in both theoretical study and engineering application. This paper presents a systematic numerical investigation on effects of deflection angles(30°, 60°, 90°, 120°, 150°, and 180°) on flow phenomena and their evolution in open-channel bends using a Re-Normalization Group(RNG) κ-ε model and a volume of fluid(VOF) method. The numerical results indicate that the deflection angle is a key factor for flows in bends. It is shown that the maximum transverse slope of water surface occurs at the middle cross section of a bend, and it increases with the deflection angle. Besides a major vortex, or, the primary circulation cell near the channel bottom, a secondary vortex, or, an outer bank cell, may also appear above the former and near the outer bank when the deflection angle is sufficiently large, and it will gradually migrate towards the inner bank and evolve into an inner bank cell. The strength of the secondary circulations increases with the deflection angle. The simulation demonstrates that there is alow-stress zone on the bed near the outer bank and a high-stress zone on the bed near the inner bank, and both of them increase in size with the deflection angle. The maximum of shear stress on the inner bank increases nonlinearly with the angle, and its maximums on the outer bank and on the bed take place when the deflection angle becomes 120°.

关键词： Open channel Deflection angle Transverse slope of water surface Secondary flow Shear stress

Abdominal ventral rectopexy with colectomy for obstructed defecation syndrome:An alternative option for selected patients

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2020年第23期8卷 5976-5987页

作者： li Wang Chun-Xue li Yue Tian Jing-Wang Ye Fan li Wei-dong Tong Department of General Surgery Daping HospitalArmy Medical UniversityChongqing 400042China

BACKGROUND Abdominal ventral rectopexy(AVR)with colectomy is controversial in the treatment of obstructed defecation syndrome(ODS).literature data on this technique for ODS are very *** To evaluate the safety and efficacy of AVR with colectomy for selected patients with *** Consecutive patients who underwent AVR with colectomy for ODS were identified prospectively from 2016 to 2017 in our *** demographics,perioperative surgical results,and postoperative follow-up outcomes were collected and ***-term follow-up was evaluated with standardized *** severity of symptoms was assessed by the objective Wexner Constipation Score(WCS)and ODS *** quality of life was assessed by the Patients Assessment of Constipation Quality of life *** outcome was compared pre-and post-operatively for each *** primary outcomes were determined by the improvement in symptoms and quality of *** outcome measures were operating time,postoperative length of stay,morbidity and mortality,improvement of pelvic floor structure,and patient *** Four patients underwent robotic-assisted surgery,and two patients underwent a laparoscopic-assisted *** mean operating time for the robotic approach was 243 min(range 160–300 min),and the mean operating time for the laparoscopic approach was 230 min(range 220-240 min).The mean postoperative length of stay was 8.2 d(range 6-12 d).There was no conversion to open procedure and no postoperative *** urinary retention,wound infection,prolonged ileus,pelvic infection and anastomosis leakage *** patients were followed up for 36 *** WCS,ODS,and Patients Assessment of Constipation Quality of life score improved significantly postoperatively(Plization at 6 to 12 mo after *** was no recurrence or novel constipation after *** of the patients used laxative

关键词： Obstructed defecation syndrome Ventral mesh rectopexy Colectomy Internal rectal prolapse Constipation Laparoscopic resection rectopexy

Malnutrition in Relation with Dietary, Geographical, and Socioeconomic Factors among Older Chinese

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2021年第5期34卷 337-347页

作者： ZHANG Jian SONG Peng Kun ZHAO li Yun SUN Ye YU Kai YIN Jing PANG Shao Jie liU Zhen MAN qing qing HE li li Cheng ARIGONI Fabrizio BOSCO Nabil DING Gang Qiang ZHAO Wen Hua National Institute of Nutrition and Health Chinese Center for Disease Control and PreventionBeijing 100050China Nestlw Research Singapore Hub 138567Singapore Nestle Research Center China Beijing 100102China Nestle Research Center Lausanne 1000LausanneSwitzerland

Objective Nutrition is closely related to the health of the elderly population. This study aimed to provide a comprehensive picture of the nutrition status of elderly Chinese and its related dietary,geographical, and socioeconomic *** A total of 13,987 ≥ 60-year-old persons from the 2010–2013 Chinese National Nutrition and Health Survey were included to evaluate various aspects of malnutrition, including underweight,overweight or obesity, and micronutrient *** Overall, the prevalence of obesity, overweight, and underweight was 12.4%, 34.8%, and 5.7%,respectively, with disparities both geographically and socioeconomically. The prevalence of underweight was higher among the older old(≥ 75 years), rural residents and those with low income, with low education status, and residing in undeveloped West areas. More than 75% of the elderly do not meet the Dietary Reference Intakes for vitamins A, B1, B2, and E, folate, calcium, selenium, potassium, biotin,and choline, with the prevalence of inadequate intake increasing with age for most nutrients. At the population level, the mean intakes of numerous food groups did not meet the recommendations by the Chinese Dietary *** Obesity epidemic, inadequacy of micronutrient intake, and high prevalence of underweight and anemia in susceptible older people are the major nutrition challenges for the rapidly aging population in China.

关键词： Malnutrition Older Chinese Food intake Nutrients National survey

PatU3 plays a central role in coordinating cell division and differentiation in pattern formation of filamentous cyanobacterium Nostoc *** 7120

在线全文

学校读者我要写书评

暂无评论

Science China(life Sciences) 2023年第12期66卷 2896-2909页

作者： Lei Yin Zhenggao Zheng Yilin li Xiying li Dan Cheng Chunxia dong Yixuan liu Jindong Zhao State Key Laboratory of Protein and Plant Genetic Engineering School of Life SciencesPeking UniversityBeijing 100871China National Teaching Center for Experimental Biology School of Life SciencesPeking UniversityBeijing 100871China Institute of Hydrobiology Chinese Academy of SciencesWuhan 430072China

Spatial periodic signal for cell differentiation in some multicellular organisms is generated according to Turing's principle for pattern *** a dividing cell responds to the signal of differentiation is addressed with the filamentous cyanobacterium Nostoc *** 7120,which forms the patterned distribution of *** show that differentiation of a dividing cell was delayed until its division was completed and only one daughter cell became heterocyst.A mutant of patU3,which encodes an inhibitor of heterocyst formation,showed no such delay and formed heterocyst pairs from the daughter cells of cell division or dumbbell-shaped heterocysts from the cells undergoing *** patA mutant,which forms heterocysts only at the filament ends,restored intercalary heterocysts by a single nucleotide mutation of patU3,and double mutants of patU3/patA and patU3/hetF had the phenotypes of the patU3 *** provide evidence that HetF,which can degrade PatU3,is recruited to cell divisome through its C-terminal domain.A HetF mutant with its N-terminal peptidase domain but lacking the C-terminal domain could not prevent the formation of heterocyst pairs,suggesting that the divisome recruitment of HetF is needed to sequester HetF for the delay of differentiation in dividing *** study demonstrates that PatU3 plays a key role in celldivision coupled control of differentiation.

关键词： cyanobacteria heterocyst differentiation cell division pattern formation PatU3

Src mediatesβ-adrenergic receptor induced YAP tyrosine phosphorylation

在线全文

学校读者我要写书评

暂无评论

Science China(life Sciences) 2020年第5期63卷 697-705页

作者： Wenjing Wang Wenqi li Kai liu Xiaodou Niu Kaihang Guan Yunqi Jiang Zijian li Erdan dong Department of Cardiology and Institute of Vascular Medicine Peking University Third Hospital/Key Laboratory of Cardiovascular Molecular Biology and Regulatory PeptidesMinistry of Health/Key Laboratory of Molecular Cardiovascular SciencesMinistry of Education/Beijing Key Laboratory of Cardiovascular Receptors ResearchBeijing 100191China Department of Pharmacy Peking University Third HospitalBeijing 100191China

The Hippo pathway is a newly identified pathway and evolutionarily conserved from flies to humans mainly regulating cell *** co-activator Yes-associated protein(YAP)functions as a major downstream effector and key node of the Hippo *** of YAP is critical to regulate YAP activity and its corresponding functions.β-adrenergic receptor(β-AR),a typical G protein coupled receptor(GPCR),mediates proliferation in various cell types and regulates multiple physical and pathological ***,the role ofβ-AR in regulating YAP remains ***,we report thatβ-AR can obviously stimulate YAP tyrosine *** mechanism is thatβ-AR stimulation results in tyrosine kinase Src activation and Src phosphorylates YAP tyrosine at *** studies demonstrate that inhibition of Src kinase activity can obviously alleviateβ-AR induced YAP tyrosine phosphorylation and cell *** conclude thatβ-AR can induce YAP tyrosine phosphorylation and also establish the Src/YAP pathway as a critical signaling branch downstream of GPCR.

关键词： YAP Hippo pathway β-adrenergic receptor tyrosine phosphorylation proliferation