检索结果-南通市图书馆

NON-SYNDROMIC HEARING LOSS AND HIGH- THROUGHPUT STRATEGIES TO DECIPHER ITS GENETIC HETEROGENEITY

Journal of Otology 2013年第1期8卷 6-24页

作者： Liu Xue Zhong Shan Kun qing jing Cheng jing YanDenise Department of Otolaryngology University of Miami The Fifth Affiliated Hospital Xinjiang Medical University Medical Systems Biology Research Center School of MedicineTsinghua University

Hearing loss (HL) is the most common sensory disorder, affecting all age groups, ethnicities, and gen-ders. According to World Health Organization (WHO) estimates in 2005, 278 million people worldwide have moderate to profound HL in both ears. Results of the 2002 National Health Interview Survey indicate that nearly 31 million of all non-institutionalized adults (aged 18 and over) in the United States have trouble hearing. Epidemiological studies have estimated that approximately 50%of profound HL can be attributed to genetic causes. With over 60 genes implicated in nonsyndromic hearing loss, it is also an extremely het-erogeneous trait. Recent progress in identifying genes responsible for hearing loss enables otolaryngologists and other clinicians to apply molecular diagnosis by genetic testing. The advent of the $1000 genome has the potential to revolutionize the identification of genes and their mutations underlying genetic disorders. This is especially true for extremely heterogeneous Mendelian conditions such as deafness, where the muta-tion, and indeed the gene, may be private. The recent technological advances in target-enrichment methods and next generation sequencing offer a unique opportunity to break through the barriers of limitations im-posed by gene arrays. These approaches now allow for the complete analysis of all known deafness-causing genes and will result in a new wave of discoveries of the remaining genes for Mendelian disorders. This re-view focuses on describing genotype-phenotype correlations of the most frequent genes including GJB2, which is responsible for more than half of cases, followed by other common genes and on discussing the im-pact of genomic advances for comprehensive genetic testing and gene discovery in hereditary hearing loss.

关键词： GJB THROUGHPUT STRATEGIES TO DECIPHER ITS GENETIC HETEROGENEITY NON-SYNDROMIC HEARING LOSS AND HIGH gene

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microscopic transduodenal excision of an ampullary adenoma:A case report and review of the literature

引用

World Journal of Clinical Cases 2021年第18期9卷 4844-4851页

作者： Xiang Zheng qing-jing Sun Bo Zhou Ming Jin Sheng Yan Department of General Surgery The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009Zhejiang ProvinceChina The Affiliated Hospital of Stomatology School of StomatologyZhejiang University School of Medicineand Key Laboratory of Oral Biomedical Research of Zhejiang ProvinceHangzhou 310006Zhejiang ProvinceChina

BACKGROUND Transduodenal local excision is an alternative treatment approach for benign ampullary ***,this procedure has technical difficulties,especially during reconstruction of the pancreaticobiliary *** operating microscope has been widely used by surgeons for delicate surgery due to its major advantages of magnification,illumination,and stereoscopic *** application of an operating microscope in transduodenal excision of ampullary tumors has not been *** SUMMARY A 55-year-old woman was admitted for investigation of recurrent upper abdominal *** examination and laboratory tests found no *** identified a large mass in the descending part of the *** revealed a 3.5-cm-sized villous growth over the major duodenal *** of the endoscopic biopsy indicated a villous adenoma with low-grade *** transduodenal excision of the ampullary tumor was *** final pathological diagnosis was villous-tubular adenoma with low-grade *** patient was discharged on postoperative day 12 after an uneventful *** retrograde cholangiopancreatography was performed 3 mo postoperatively and showed no bile duct or pancreatic duct strictures and no tumor *** patient is continuing follow-up at our clinic and remains *** Operating microscope-assisted transduodenal local excision is a feasible and effective option for benign ampullary tumors.

关键词： Operating microscope Transduodenal local excision Ampullary tumor Endoscopic papillectomy Pancreaticoduodenectomy Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Practical Stability Analysis and Synthesis of Linear Descriptor Systems with Disturbances

引用

International Journal of Automation and computing 2008年第2期5卷 138-144页

作者： Chun-Yu Yang Xin jing qing-Ling Zhang Lin-Na Zhou Institute of Systems Science Key Laboratory of Integrated Automation of Process Industry Ministry of Education~ Northeastern University Shenyang 110004 PRC

This paper considers the problems of practical stability analysis and synthesis of linear descriptor systems subject to timevarying and norm-bounded exogenous disturbances. A sufficient condition for the systems to be regular, impulsive-free and practically stable is derived. Then the synthesis problem is addressed and a state feedback controller is designed. To deal with the computational issue, the conditions of the main results are converted into linear matrix inequality （LMI） feasibility problems. Furthermore, two optimization algorithms are formulated to improve the system performances. Finally, numerical examples are given to illustrate the obtained results.

关键词： Linear descriptor systems disturbances practical stabilization practical stability linear matrix inequality (LMI)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第5期8卷 345-351页

作者： JIANG Li-qin LI Xu-qing ZHENG jing-wu Institute of Biotechnology School of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China

Bursaphelenchus rainulfi isolated from dead pine trees in Zbejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit （LSU） rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.

关键词： Bursaphelenchus rainulfi Pine wood Morphology Morphometrics ITS-RFLP Phylogeny

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prognostic value of the lymph node ratio in stage Ⅲ colorectal cancer

引用

Chinese Journal of Cancer 2012年第5期31卷 241-247页

作者： jing-qing Ren Jian-Wei Liu Zhi-Tang Chen Shao-Jie Liu Shi-Jie Huang Yong Huang jing-Song Hong Department of General Surgery The Fourth Affiliated Hospital of Jinan UniversityGuangzhou Red Cross HospitalGuangzhouGuangdong 510220P.R.China

The nodal stage of colorectal cancer is based on the number of positive *** is inevitably affected by the number of removed lymph nodes,but lymph node ratio can be *** investigated the value of lymph node ratio in stage Ⅲ colorectal cancer in this *** clinicopathologic factors and follow-up data of 145 cases of stage Ⅲ colorectal cancer between January 1998 and December 2008 were analyzed *** Pearson and Spearman correlation analyses were used to determine the correlation coefficient,the Kaplan-Meier method was used to analyze survival,and the Cox proportional hazard regression model was used for multivariate analysis in forward stepwise *** found that lymph node ratio was not correlated with the number of removed lymph nodes(r =-0.154,P = 0.065),but it was positively correlated with the number of positive lymph nodes(r = 0.739,P < 0.001) and N stage(r = 0.695,P < 0.001).Kaplan-Meier survival analysis revealed that tumor configuration,intestinal obstruction,serum carcinoembryonic antigen(CEA) concentration,T stage,N stage,and lymph node ratio were associated with disease-free survival of patients with stage Ⅲ colorectal cancer(P < 0.05).Multivariate analysis showed that serum CEA concentration,T stage,and lymph node ratio were prognostic factors for disease-free survival(P < 0.05),whereas N stage failed to achieve significance(P = 0.664).We confirmed that lymph node ratio was a prognostic factor in stage Ⅲ colorectal cancer and had a better prognostic value than did N stage.

关键词：淋巴结大肠癌预后价值多元统计分析多因素分析病理因素相关分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enterocyte dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin expression in inflammatory bowel disease

引用

World Journal of Gastroenterology 2015年第1期21卷 187-195页

作者： jing-qing Zeng Chun-Di Xu Tong Zhou jing Wu Kai Lin Wei Liu Xin-Qiong Wang Department of Pediatrics Ruijin HospitalShanghai Jiao Tong University School of Medicine Department of Pediatrics Shanghai Children’s Medical CenterShanghai Jiao Tong University School of Medicine

AIM: To investigate dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin(DC-SIGN) expression in intestinal epithelial cells(IECs) in inflammatory bowel disease(IBD).METHODS: The expression of DC-SIGN in IECs was examined by immunohistochemistry of intestinal mucosal biopsies from 32 patients with IBD and 10 *** activity indices and histopathology scores were used to assess the tissue lesions and pathologic *** studies utilized BALB/c mice with dextran sodium sulfate(DSS)-induced colitis treated with anti-P-selectin lectin-EGF domain monoclonal antibody(PsL-EGFmA b).Controls,untreated and treated mice were sacrificed after 7 d,followed by isolation of colon tissue and *** expression of DC-SIGN,CD80,CD86 and MHC Ⅱ was examined by immunohistochemistry or flow *** capacity of mouse enterocytes or dendritic cells to activate T cells was determined by coculture with naive CD4+ T *** supernatant and intracellular levels of interleukin(IL)-4 and interferon(IFN)-γ were measured by enzyme-linked immunosorbent assay and flow cytometry,*** ability of IECs to promote T cell proliferation was detected by flow cytometry staining with carboxyfluorescein diacetate succinimidyl ***: Compared with controls,DC-SIGN expression was significantly increased in IECs from patients with Crohn's disease(P < 0.01) or ulcerative colitis(P < 0.05).DC-SIGN expression was strongly correlated with disease severity in IBD(r = 0.48; P < 0.05).Similarly,in the DSS-induced colitis mouse model,IECs showed upregulated expression of DC-SIGN,CD80,CD86 and MHC,and DC-SIGN expression was positively correlated with disease activity(r = 0.62: P < 0.01).IECs from mouse colitis stimulated naive T cells to generate IL-4(P < 0.05).Otherwise,dendritic cells promoted a T-helper-1-skewing phenotype by stimulating IFN-γ ***,DC-SIGN expression and T cell differentiation were suppressed following treatment

关键词： Dendritic cell-specific intercellular adhesion mol

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene

引用

World Journal of Gastroenterology 2011年第14期17卷 1895-1902页

作者： Xiao-Bo Guo Chang-qing jing Le-Ping Li Li Zhang Yu- Long Shi Jin-Shen Wang jing-Lei Liu Chen-Sheng Li Department of Gastrointestinal Surgery Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Weiqi Road Jinan 250021 Shandong Province China

AIM:To evaluate the biological and clinical characteristics of miR-622 in gastric cancer. METHODS:We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in nude mice. A luciferase reporter assay was used to assess the effect of miR-622 on inhibitor of growth family,member 1 (ING1) expression. RESULTS:Expression of miR-622 was down-regulated in gastric cancer. MiR-622 was found involved in differentia-tion and lymphatic metastasis in human gastric cancer. Ectopic expression of miR-622 promoted invasion,tumorigenesis and metastasis of gastric cancer cells both in vitro and in vivo. ING1 is a direct target of miR-622. CONCLUSION:These findings help clarify the molecular mechanisms involved in gastric cancer metastasis and indicate that miR-622 modulation may be a bona fide treatment of gastric cancer.

关键词： MicroRNA MiR-622 Gastric cancer Metastasis Inhibitor of growth family member 1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

PLASMA RESISTIN LEVELS AND SINGLE-NUCLEOTIDE POLYMORPHISMS IN RESISTIN GENE 5 FLANKING REGION IN PATIENTS WITH STROKE

引用

Chinese Medical Sciences Journal 2007年第1期22卷 27-32页

作者： Xing-jian Lin Ying-dong Zhang qing-shan Guan qing Di jing-ping Shi Wei-guo Liu Department of Neurology Nanjing Brain Hospital Nanjing Medical University Nanjing 210029

Objective To analyze the role of resistin in insulin resistance (IR) through investigating the variation of plasma resistin levels and single-nucleotide polymorphisms (SNPs) in resistin gene 5’ flanking region in stroke *** In 103 atherothrombotic cerebral infarction (ACI) patients, 85 lacunar infarction (LI) patients, 70 intracerebral hemorrhage (ICH) patients, and 86 healthy controls, plasma resistin and insulin levels were measured by ELISA , SNPs in resistin gene 5’ flanking region were detected by PCR and direct DNA sequencing. The subjects’ body height and weight, the body mass index, quantitative insulin sensitivity check index (QUICKI), blood pressure, and the concentration of fasting plasma glucose, triglyceride, total cholesterol, creatinine, low-density lipoprotein, and high-density lipoprotein were also determined. Results QUICKI was significantly lower in the ACI and ICH patients (0.316±0.037 and 0.309±0.032, respectively) than that in the controls (0.342±0.043, PG and -537A>C SNPs were not significantly different among the different groups, and those SNPs were not correlated with other clinical and biochemical *** Plasma resistin is associated with stroke by participating in the development of IR. The SNPs in resistin gene 5’ flanking region has no impact on the plasma resistin level.

关键词： resistin insulin resistance single-nucleotide polymorphisms stroke

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened

引用

World Journal of Gastroenterology 2013年第14期19卷 2197-2207页

作者： Kai Sun Hai-Jun Deng Shang-Tong Lei jing-qing Dong Guo-Xin Li Department of General Surgery Nanfang Hospital of Southern Medical University

AIM:To investigate the regulative effect of miRNA-3383p(miR-338-3p) on cell growth in colorectal carcinoma(CRC).METHODS:The lentiviral vector pLV-THM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were *** recombinant viral vector encoding the pre-miR338-3p or miR-338-3p-inhibitor and the two packaging plasmids psPAX2 and pMD2.G were cotransfected into human embryonic kidney 293T cells to package *** supernatant containing the lentivirus particles was harvested to determine the viral titer,and this supernatant was then used to transduce CRCderived cell line,*** cytometry was utilized for sorting the green fluorescent protein(GFP) + cells to establish the SW-620 cell line stably expressing premiR-338-3p or ***,the expression of miR-338-3p was determined by real-time reverse transcriptase polymerase chain reaction,andWestern blotting was used to detect the expression of the smoothened(SMO,the possible target of miR-3383p) protein in SW-620 ***,the status of CRC cell proliferation and apoptosis were detected by 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay and flow cytometry,***:Restriction enzyme digestion and DNA sequencing demonstrated that the lentiviral vector pLVTHM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed *** was expressed after the SW-620 cells were transduced by the *** of miR-338-3p in SW-620 cells transduced with the lentivirus pLV-THM-miR-338-3p was significantly increased(relative expression 3.91 ± 0.51 vs 2.36 ± 0.44,P < 0.01).Furthermore,overexpression of miR-338-3p inhibited the expression of SMO protein in SW-620 cells,which showed obviously suppressed proliferation ability [cellular proliferation inhibition rate(CPIR) 61.9% ± 5.2% vs 41.6% ± 4.8%,P < 0.01].Expression of miR-338-3p in SW-620 cells transduced with the lentivirus pLV-THM-miR-338-3p-inhibitor was significantly decreased(relative ex

关键词： Colorectal carcinoma Hsa-miRNA-338-3p Smoothened Lentivirus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Absorption Cross Section of Einstein-Maxwell Dilation Axion Black Hole for Dirac Particles

引用

Communications in Theoretical Physics 2007年第12期48卷 1051-1054页

作者： LIU Chang-qing jing Ji-Liang Institute of Physics and Department of Physics,Hunan Normal University,Changsha 410081,China Institute of Physics and Department of Physics Hunan Normal University Changsha 410081 China

The absorption cross section of the Einstein-Maxwell Dilaton Axion (EMDA) black hole for Dirac particlesis *** is shown that the absorption cross section decreases as both the v and the absolute value of the dilatonincrease,but it increases as the mass of the particle *** is also shown that the absorption cross section for themassless Dirac particles is 1/8 of the area of the horizon,which may be an universal property for minimally coupledmassless Dirac particles.

关键词： black hole absorption cross section Dirac particle

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：