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肝螺杆菌甲基基团接受趋化信号转导蛋白的克隆表达及纯化

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胃肠病学和肝病学杂志 2009年第10期18卷 930-933页

作者：牛凌云夏国盛李菁郭蒸秦和平王继德白杨南方医科大学附属南方医院消化疾病研究所广东广州510515

目的克隆肝螺杆菌(***)甲基基团接受趋化信号转导蛋白(MCP)基因,制备MCP重组蛋白,为以后检测我国人群肝螺杆菌感染率提供实验基础。方法利用PCR方法扩增目的基因片段,连接至原核表达载体pET22b(+),构建MCP的原核表达质粒pET22b+/MCP。... 详细信息

目的克隆肝螺杆菌(***)甲基基团接受趋化信号转导蛋白(MCP)基因,制备MCP重组蛋白,为以后检测我国人群肝螺杆菌感染率提供实验基础。方法利用PCR方法扩增目的基因片段,连接至原核表达载体pET22b(+),构建MCP的原核表达质粒pET22b+/MCP。将质粒转化入大肠杆菌BL21(DE3)进行诱导表达,通过Ni-NTA亲和层析系统纯化重组蛋白。结果构建了原核表达质粒pET22b+/MCP,含有该质粒的大肠杆菌经1 mmol/L IPTG诱导4 h后表达一相对分子质量约14 kD的重组蛋白,经Ni-NTA亲和层析系统纯化获得了MCP的纯化重组蛋白rhMCP。结论获得了肝螺杆菌MCP的纯化重组蛋白,为进一步利用血清学方法调查我国人群肝螺杆菌感染率提供可能。

关键词：肝螺杆菌甲基基团接受趋化信号转导蛋白克隆重组蛋白

Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella

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Science China(Life Sciences) 2013年第1期56卷 59-65页

作者： CHEN CongLin SUN XiaoYun LIAO LanJie LUO ShaoXiang LI ZhouQuan ZHANG XiaoHua WANG YaPing GUO QionLin FANG qin DAI heping State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of Sciences

Grass carp （Ctenopharyngodon idella） is an important species of freshwater aquaculture fish in China. However, grass carp reovirus （GCRV） can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic ca- pacity of the virus＇s structural proteins for preparing an effective vaccine has not been available. In this study, some sin- gle-chain variable fragment antibodies （scFv）, which could specifically recognize grass carp IgM, were selected from a con- structed mouse naive antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from vi- rus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.

关键词： grass carp grass carp reovirus (GCRV) IgM single-chain variable fragment (scFv) antigenicity

Foundation Treatments for Embankments over Highly Compressible Peat in CKE Project

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Journal of Civil Engineering and Architecture 2011年第12期5卷 1138-1141页

作者： Qi Zhang Xiaqiang qin heping Dong Xia Yan China Metallurgical Group Corporation （MCC） CKE Project Office No. 97Awarakotuwa Kerawalapitiya Wattala Sri Lanka China Metallurgical Group Corporation （MCC） No. 777 Pangu Road Baoshan District ShanghaL China

The construction of CKE road embankments is undertaken over soft soils, which include peats, organic soils, clays and sludge. The moisture content of peat is up to 700% and void ratio is up to 11.99. It＇s very easy for the embankments of high moisture content, high void ratio and of the high compression nature to cause too much settlement and loss of stability. Embankment stability during construction and residual settlement of pavement during service period are two major challenges to be faced of the design and construction. This paper outlines the site investigation, laboratory tests and monitoring data in a few selected existing preloading areas, and presents the back-analysis results of the modified secondary compression indices of in-situ soft materials. It is found that the early monitoring data after primary settlement completion are a more reliable approach to estimate the residual settlements within a nominated duration.

关键词： Highly compressible peat study of residual settlement.

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壳聚糖基卡托普利的合成及其释药研究

湖南大学学报（自然科学版） 2018年第6期45卷 145-149页

作者：李和平肖青秦龙长沙理工大学化学与生物工程学院湖南长沙410114 云南水利水电职业学院基础教育部云南昆明650499

克服水溶性药物的突释现象,可减少给药次数,提高疗效.本研究以卡托普利为模型药物,壳聚糖为载体,采用耦合的方法,设计合成了壳聚糖-卡托普利和壳聚糖-赖氨酰-卡托普利两种新的键连型壳聚糖基载药体系,产物结构经IR、1 H NMR和MS表征;并... 详细信息

克服水溶性药物的突释现象,可减少给药次数,提高疗效.本研究以卡托普利为模型药物,壳聚糖为载体,采用耦合的方法,设计合成了壳聚糖-卡托普利和壳聚糖-赖氨酰-卡托普利两种新的键连型壳聚糖基载药体系,产物结构经IR、1 H NMR和MS表征;并测试了两种化合物在PBS缓冲溶液(pH=7.4)和HCl-KCl缓冲溶液(pH=1.2)中72h的累积释药率:壳聚糖-卡托普利的累积释药率分别为59.2%和78.4%,壳聚糖-赖氨酰-卡托普利的累积释药率分别为55.2%和76.4%.结果表明,两种键连型载药体系均消除了突释现象,具有很好的缓释效果,这将有望成为水溶性药物的理想载药体系.

关键词：卡托普利壳聚糖合成缓释

IL-17A-dependent gut microbiota is essential for regulating diet-induced disorders in mice

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Science Bulletin 2017年第15期62卷 1052-1063页

作者： Yujing Bi Chunxiao Li Lin Liu Jiyuan Zhou Zhengchao Li Huimin Deng Chunyan Wu Yanping Han Yajun Song Yafang Tan Xiaoyi Wang Zongmin Du Yujun Cui Yanfeng Yan Fachao Zhi Guangwei Liu Nan qin heping Zhang Ruifu Yang State Key laboratory of Pathogen and Biosecurity. Beijing Institute of MicrobiologY and Epidemiology Beijing 100071 China Key Laboratory of Cell Proliferation and Regulation Biology Ministry of Education Institute of Cell Biology College of Life Science Beijing Normal University Beijing 100875 China State Key Laboratory for Diagnosis and Treatment of Infectious Disease The First Affiliated Hospital College of Medicine Zhejiang University Hangzhou 310003 China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang University Hangzhou 310003 China Guangdong Provincial Key Laboratory of Gastroenterology Institute of Gastroenterology of Guangdong Province Department of Gastroenterology Nan fang Hospital Southern Medical University Guangzhou 510515 China Realbio Genomics Institute Shanghai 200050 China Key Laboratory of Dairy Biotechnology and Engineering Education Ministry of China Inner Mongolia Agricultural University Hohhot 010018 China

The gut microbiota plays a key role in obesity and related metabolic disorders, and multiple factors including diet, host genotype, and age regulate it. Many studies have examined the contribution of extrinsic factors to the regulation of the gut microbiota, but the importance of the host genetic constitution cannot be ignored, lnterleukin 17A （lL-17A）, a pro-inflammatory cytokine, is important in the defense against infection and diseases. Here, we investigated the association among IL-17, a high-fat diet （HFD）, and the gut microbiota. Mice deficient in 1L-17A were resistant to diet-induced obesity and related diseases. Compared with the I1-17a^-/1 mice, wild-type （WT） mice challenged with HFD showed obvious weight fluctuations, such as those seen in type 2 diabetes, and hematological changes similar to those associated with metabolic syndrome. However, housing WT mice and Il-17a^-/- mice together signifi- cantly alleviated these symptoms in the WT mice. A metagenomic analysis of the mouse feces indicated that the microbial community compositions of these two groups differed before HFD feeding. The HFD mediated shifts in the gut microbial compositions, which were associated with the mouse phenotypes. We also identified potentially beneficial and harmful species present during this period, and drew net- works of the most abundant species. A functional analysis indicated pathway changes in the WT and I1-17a^- /- mice when fed the HFD. Collectively, these data underscore the importance of the host factor IL-17A in shaping and regulating the gut microbiota, which conversely, influences the host health.

关键词： Gut microbiota Obesity IL-17A Metagenomics

Glyphosate resistance in Tridax procumbens endowed by a novel EPSPS Thr-102-Ser mutation

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Glyphosate resistance in Tridax procumbens endowed by a nove...

第十三届全国杂草科学大会

作者： Jingbo Li Qiong Peng heping Han qin Yu Stephen B.Powles Hunan University of Humanities Science and Technology Australian Herbicide Resistance Initiative University of Western Australia Hunan Academy of Agricultural Sciences

The tropical Tridax procumbens is a global weed of 31 crops in 60 *** Australia,it is mostly found in Northern Australia,infesting vegetables,pasture,irrigated and rainfed ***,a *** population from Kununurra,Western Australia was suspected to have evolved glyphosate *** population had been managed with repeated glyphosate treatments over several years with poor control only recently *** dose response experiments confirmed this population with low-level(3-4 fold) glyphosate *** mechanism studies found that resistance was not due to non-target-site reduced glyphosate uptake or translocation,nor due to target-site EPSPS ***,sequencing and cloning of a 998 bp EPSPS cDNA fragment covering potential mutation sites from resistant vs susceptible plants revealed a new point mutation at amino acid position 102(ACC to TCC),resulting in Thr-102-Ser *** novel mutation is very likely responsible for the low-level glyphosate resistance in this *** Thr-102 is within the strictly conserved region in EPSPS,close to glyphosate binding site and crucial for EPSPS catalytic *** mutations at this Thr-102 site can endow high-level glyphosate resistance but reduce EPSPS catalytic efficiency unless compensated by other concurrent mutation(s),such as the recently discovered double Thr-102-Ue/Pro-106-Ser(TIPS) mutation in Eleusine ***,the substitution of Thr-102 by the Ser residue(similar polarity to Thr and smaller than Ile) would likely be sufficient for endowing glyphosate resistance with minimum reduction of catalytic *** addition,the ploidy was determined to be tetraploid and two EPSPS genes confirmed for the resistant and susceptible *** plants used in this *** work underway involves cloning full EPSPS sequences and understanding the structural basis(3D) of glyphosate resistance.

关键词： glyphosate resistance mechanism target site mutation

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解一类最优控制问题的一种自适应拟谱方法

信息与控制 2012年第5期41卷 583-589页

作者：秦廷华马和平上海大学理学院数学系上海200444

提出一种解常微分方程最优控制问题的自适应拟谱方法.对状态函数和控制函数使用不同次数的多项式逼近.设计了一个自适应算法决定不同的次数,并给出了理论分析.数值算例比较了本文方法与一种常用的拟谱方法.

关键词：拟谱方法最优控制非线性规划自适应

The influence of microwave energy on the temperature field distribution and properties evolution characteristics of cement mortar containing magnetite

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Journal of Traffic and Transportation Engineering(English Edition) 2024年

作者： heping Qiu Yongchang Wu Huaxin Chen Yimin Liu Yujin Yao Jiayu Wu Doctor candidate.School of Materials Science and Engineering， Chang'an University School of Materials Science and Engineering， Chang'an University School of Civil and Transportation Engineering， Ningbo University of Technology

To improve the microwave absorption ability of cement mortar， this study focused on the preparation of magnetite cement mortar (MCM) with magnetite as the main absorbing material. The electromagnetic (EM) parameters of MCM were measured and the microwave reflection loss (RL) was calculated to study its microwave absorption efficiency. Meanwhile， the thermal response of MCM was recorded using an infrared thermal imager to analyze its heating and temperature field distribution characteristics. In addition， to study the mechanical properties of MCM at different microwave heating times from macroscopic and microscopic scale， the press machine， SEM， XRD， and MIP were used. The results showed that increasing the magnetite content could effectively improve the microwave absorption efficiency of MCM. After 240s of microwave heating， the surface temperature of MCM with 25% magnetite content was up to 184.9 ℃， compared to the surface temperature of 162.6 ℃ in the control group. In the same microwave heating time， although adding magnetite significantly increased the surface temperature of MCM， it caused an increase in the variability of the MCM temperature field distribution， which might generate a certain temperature gradient stress. The maximum surface temperature difference of the control group was 59.9 ℃， which was 14.3 ℃ smaller than that of the MCMwith 25% magnetite content. The continuous temperature difference generated attenuated the mechanical and physicochemical properties of the MCM. The compressive and flexural strength of MCM with 25% magnetite content was deterioration about 4.1 and 0.9 MPa， respectively. Although the MCM had favorable heating capability for pavement deicing， its engineering properties deterioration degree should be considered during long-term microwave heating.

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辊道窑烧成带气体流场与温度场的三维数值模拟

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中国陶瓷 2006年第5期42卷 27-31页

作者：童剑辉冯青汪和平景德镇陶瓷学院景德镇333001

通过数值模拟的方法,利用C F D软件FLUENT6.0,建立实体计算模型并采用六面体结构与非结构混合网格对模型进行网格划分;采用标准K-ε湍流模型和P-1辐射模型对辊道窑烧成带内气体流场与温度场进行了数值模拟研究。得出了窑内气体流场和温... 详细信息

通过数值模拟的方法,利用C F D软件FLUENT6.0,建立实体计算模型并采用六面体结构与非结构混合网格对模型进行网格划分;采用标准K-ε湍流模型和P-1辐射模型对辊道窑烧成带内气体流场与温度场进行了数值模拟研究。得出了窑内气体流场和温度场的分布,分析了流场对窑内气体温度场和对流换热的影响。

关键词：辊道窑 K-ε 湍流模型数值模拟流场温度场