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Physiological,transcriptome and gene functional analysis provide novel sights into cadmium accumulation and tolerance mechanisms in kenaf

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Journal of Environmental Sciences 2024年第3期137卷 500-514页

作者： Shan Cao Meng Wang Jiao Pan Dengjie Luo Samavia Mubeen Caijin Wang Jiao Yue Xia wu qijing wu Hui Zhang Canni Chen Muzammal Rehman Sichen Xie Ru Li Peng Chen Guangxi Key Laboratory of Agro-environment and Agric-products safety Key Laboratory of Plant Genetics and BreedingCollege of AgricultureGuangxi UniversityNanning 530004China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China

Kenaf is considered to have great potential for remediation of heavy metals in ***,studies on molecular mechanisms of root Cd accumulation and tolerance are still *** this study,two differently tolerant kenaf cultivars were selected as materials and the physiological and transcriptomic effects were evaluated under Cd *** study showed that 200μmol/L CdCl_(2) treatment triggered the reactive oxygen species(ROS)explosion and membrane lipid *** with the Cd-sensitive cultivar‘Z',the Cd-tolerant cultivar‘F'was able to resist oxidative stress in cells by producing higher antioxidant enzyme activities and increasing the contents of ascorbic acid(AsA)and glutathione(GSH).The root cell wall of‘F'exhibited higher polysaccharide contents under Cd treatment,providing more Cd-binding *** were 3,439 differentially expressed genes(DEGs)that were co-regulated by Cd treatment in two *** biosynthesis and plant hormone signal transduction pathways were significantly enriched by functional annotation *** associated with pectin,cellulose,and hemi-cellulose metabolism were involved in Cd chelation of root cell wall;V-ATPases,ABCC3 and Narmp3 could participated in vacuolar compartmentalization of Cd;PDR1 was responsible for Cd effux;the organic acid transporters contributed to the absorption of Cd in *** genes might have played key roles in kenaf Cd tolerance and Cd ***,HcZIP2 was identified to be involved in Cd uptake and transport in *** findings provide a deeper understanding of the molecular pathways underlying Cd accumulation and detoxification mechanisms in kenaf.

关键词： Kenaf Cd stress Physiological Transcriptome HcZIP2

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庭审事实重构中律师说服行为的功能语义构型

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现代外语 2024年第2期47卷 197-208页

作者：吴启竞大连理工大学辽宁省大连市116024

庭审事实重构中律师经验意义的传递是律师说服行为的基础,这些意义由功能语义构型表征。本研究结合系统功能语言学中的核心关系和律师说服行为的语境因素搭建分析框架,探讨庭审事实重构中律师说服行为的功能语义构型及其说服作用。通过... 详细信息

庭审事实重构中律师经验意义的传递是律师说服行为的基础,这些意义由功能语义构型表征。本研究结合系统功能语言学中的核心关系和律师说服行为的语境因素搭建分析框架,探讨庭审事实重构中律师说服行为的功能语义构型及其说服作用。通过对自建庭审话语语料库进行质性标注和量化分析,本研究发现了律师说服行为的六种典型功能语义构型,它们在特定庭审阶段中呈现出使用倾向。由于这些语义构型能够清晰表明律师争取审判人员认同的目的,适合所在庭审阶段且符合律师职业伦理规范,因此具有很好的说服作用。

关键词：事实重构律师说服行为功能语义构型核心关系

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Understory seedlings of Quercus mongolica survive by phenological escape

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Forest Ecosystems 2024年第2期11卷 236-246页

作者： Shixiong wu Ying Liu Lulu He Wei Zeng qijing Liu College of Forestry Beijing Forestry UniversityBeijing100083China

Understanding understory seedling regeneration mechanisms is important for the sustainable development of temperate primary forests in the context of increasingly intense climate warming *** poor regeneration of dominant tree species,however,is one of the biggest challenges it faces at the ***,the regeneration of the shade-intolerant Quercus mongolica seedling is difficult in primary forests,which contrasts with the extreme abundance of understory seedlings in secondary *** mechanism behind the interesting phenomenon is still *** study used in-situ monitoring and nursery-controlled experiment to investigate the survival rate,growth performance,as well as nonstructural carbohydrate (NSC) concentrations and pools of various organ tissues of seedlings for two consecutive years,further analyze the understory light availability and simulate the foliage carbon (C) gain in the secondary and primary *** suggested that seedlings in the secondary forest had greater biomass allocation aboveground,height and specific leaf area (SLA) in summer,which allowed the seedling to survive longer in the canopy closure *** light availability and positive C gain in early spring and late autumn are key factors affecting the growth and survival of understory seedlings in the secondary forest,whereas seedlings in the primary forest had annual negative carbon *** the growing season,the total NSC concentrations of seedlings gradually decreased,whereas those of seedlings in the secondary forest increased significantly in autumn,and were mainly stored in roots for winter consumption and the following year's summer shade period,which was verified by the nursery-controlled experiment that simulated autumn enhanced light availability improved seedling survival rate and NSC *** conclusion,our results revealed the survival trade-off strategies of Quercus mongolica seedlings and highlighted the necessity of high light availability d

关键词： Carbon gain Nonstructural carbohydrate Recruitment Trade-off Biomass allocation

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Engineering Shewanella-re duce d graphene oxide aerogel biohybrid to efficiently synthesize Au nanoparticles

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Journal of Materials Science & Technology 2024年第1期168卷 50-61页

作者： Baocai Zhang Shulin Shao Huan Yu qijing Liu Sicheng Shi Qingyuan wu Zijie wu Yiyu Feng Yifei Wang Xi Sun Deguang wu Feng Li Hao Song Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical TechnologyBeijing 100029China School of Materials Science and Engineering Tianjin UniversityTianjin 300350China College of Biological Engineering Tianjin Agricultural UniversityTianjin 300384China Department of Brewing Engineering Moutai InstituteLuban AveRenhuai 564507China

Biosynthesizing Au nanoparticles(AuNPs)from gold-bearing scraps provides a sustainable method to meet the urgent demand for ***,it remains challenging to efficiently biosynthesize AuNPs of which the diameter is less than 10 nm from a trace amount of Au^(3+)concentration at the level of tens ***,we constructed an exoelectrogenic cell(eCell)-conductive reduced-graphene-oxide aero-gel(rGA)biohybrid by assembling Shewanella sp.S1(SS1)as living biocatalyst and rGA as conductive ad-sorbent,in which Au^(3+)at trace concentrations would be enriched by the adsorption of rGA and reduced to AuNPs through the extracellular electron transfer(EET)of *** regulate the size of the synthe-sized AuNPs to 10 nm,the strain SS1 was engineered to enhance its EET,resulting in strain RS2(pYYD-P tac-ribADEHC&pHG13-P_(bad)-omcC in SS1).Strain RS2 was further assembled with rGA to construct the RS2-rGA biohybrid,which could synthesize AuNPs with the size of 7.62±2.82 nm from 60 ppm Au^(3+)*** eCell-rGA biohybrid integrated Au^(3+)adsorption and reduction,which enabled AuNPs biosyn-thesis from a trace amount of Au^(3+).Thus,the required Au^(3+)ions concentration was reduced by one or two orders of magnitude compared with conventional methods of AuNPs *** work devel-oped an AuNPs size regulation technology via engineering eCell’s EET with synthetic biology methods,providing a feasible approach to synthesize AuNPs with controllable size from trace level of gold ions.

关键词： Au nanoparticles Exoelectrogenic cell(e-Cell) Shewanella Synthesis biology Extracellular electron transfer Reduced graphene oxide aerogel

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Glutamine metabolic microenvironment drives M2 macrophage polarization tomediate trastuzumab resistance in HER2-positive gastric cancer

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Cancer Communications 2023年第8期43卷 909-937页

作者： Xingbin Hu Zhenfeng Ma Beibei Xu Shulong Li Zhiqi Yao Bishan Liang Jiao Wang Wangjun Liao Li Lin Chunling Wang Siting Zheng qijing wu Qiong Huang Le Yu Fenghua Wang Min Shi Department of Oncology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdongP.R.China School of Biomedical Engineering Southern Medical UniversityGuangzhouGuangdongP.R.China School of Pharmaceutical Sciences Southern Medical UniversityGuangzhouGuangdongP.R.China Department of Medical Oncology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China

Background:Trastuzumab is a first-line targeted therapy for human epidermal growth factor receptor-2(HER2)-positive gastric ***,the inevitable occurrence of acquired trastuzumab resistance limits the drug benefit,and there is currently no effective reversal *** researches on the mechanism of trastuzumab resistance mainly focused on tumor cells themselves,while the understanding of the mechanisms of environment-mediated drug resistance is relatively *** study aimed to further explore the mechanisms of trastuzumab resistance to identify strategies to promote survival in these ***:Trastuzumab-sensitive and trastuzumab-resistant HER2-positive tumor tissues and cells were collected for transcriptome *** were used to analyze cell subtypes,metabolic pathways,and molecular signaling *** in microenvironmental indicators(such as macrophage,angiogenesis,and metabolism)were verified by immunofluorescence(IF)and immunohistochemical(IHC)***,a multi-scale agent-based model(ABM)was *** effects of combination treatment were further validated in nude mice to verify these effects predicted by the ***:Based on transcriptome sequencing,molecular biology,and in vivo experiments,we found that the level of glutamine metabolism in trastuzumabresistant HER2-positive cells was increased,and glutaminase 1(GLS1)was significantly ***,tumor-derived GLS1 microvesicles drove M2macrophage ***,angiogenesis promoted trastuzumab *** showed high glutamine metabolism,M2 macrophage polarization,and angiogenesis in trastuzumab-resistant HER2-positive tumor tissues from patients and ***,the cell division cycle 42(CDC42)promoted GLS1 expression in tumor cells by activating nuclear factor kappa-B(NF-κB)p65 and drove GLS1microvesicle secretion through IQmotif-containing GTPase-activating protein 1(IQGAP1).Based on the ABM and

关键词： Gastric cancer glutamine metabolism macrophage mathematical model microvesicles trastuzumab

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转述:对话性与潜在的交际偏误

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外语教学 2023年第2期44卷 18-22页

作者：王振华吴启竞上海交通大学外国语学院大连理工大学外国语学院

转述就是把所发生的事件、进行中的活动、事件或活动的态势,以及人们发表的言论、观点、立场等通过书写、言说、图片、音频、视频等不同媒介传播出来,是转述者介入互动的一种方式,具有对话性。转述的对话性可以促成有效交际。但若转述不... 详细信息

转述就是把所发生的事件、进行中的活动、事件或活动的态势,以及人们发表的言论、观点、立场等通过书写、言说、图片、音频、视频等不同媒介传播出来,是转述者介入互动的一种方式,具有对话性。转述的对话性可以促成有效交际。但若转述不当,则会影响交际的顺利进行。本文从转述能够介入互动的角度论证转述的对话性,并结合再语境化和语法隐喻说明转述的对话性对意义传递(再现与理解)的影响。本文认为,再语境化过程中的语境变化和语法隐喻的意义潜势可能使转述传递的意义有别于原话,引发误解、增加理解成本,甚至可能导致交际失败。

关键词：转述介入对话性再语境化语法隐喻

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足细胞衰老与肾小球疾病

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肾脏病与透析肾移植杂志 2023年第5期32卷 472-476页

作者：吴其晶杨倩倩南京医科大学附属淮安第一人民医院肾脏科淮安223300

随着人口老龄化的加重,与其相关的疾病给社会带来沉重的负担。在老龄相关疾病中,与衰老相关的肾脏疾病是其中一个重要的老年医学问题。足细胞是肾小球滤过膜的最外层屏障,其结构和功能的异常与肾脏疾病密切相关。有研究提出衰老性肾小... 详细信息

随着人口老龄化的加重,与其相关的疾病给社会带来沉重的负担。在老龄相关疾病中,与衰老相关的肾脏疾病是其中一个重要的老年医学问题。足细胞是肾小球滤过膜的最外层屏障,其结构和功能的异常与肾脏疾病密切相关。有研究提出衰老性肾小球硬化是一种“足细胞病”的概念,并已被广泛证实。近年来,越来越多地研究表明,足细胞衰老是肾脏损伤的重要环节和潜在治疗靶点。本文对足细胞衰老在肾脏损伤中的作用及机制进行综述和探讨。

关键词：肾小球疾病足细胞衰老

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全生物质α-甲壳素纳米纤维柔性透明膜的制备及性能研究

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材料导报 2023年第14期37卷 238-243页

作者：吴启静马洁茹刘硕王露臻周彤李大纲陈楚楚南京林业大学材料科学与工程学院南京210037

在自然界中,甲壳素一般以有序的结晶纳米纤维形式存在,赋予了生物体优异的力学性能。本研究分别采用“常温温和”(常温,Mild condition)和“高温加热”(高温,High temperature condition)两种方法从废弃蟹壳中提取α-甲壳素并制得常温α... 详细信息

在自然界中,甲壳素一般以有序的结晶纳米纤维形式存在,赋予了生物体优异的力学性能。本研究分别采用“常温温和”(常温,Mild condition)和“高温加热”(高温,High temperature condition)两种方法从废弃蟹壳中提取α-甲壳素并制得常温α-甲壳素纳米纤维膜(常温膜)和高温α-甲壳素纳米纤维膜(高温膜),分别对所得两种α-甲壳素纳米纤维样品从微观结构、力学性能、结晶构造、表面特性以及光学性能等方面进行表征,分析对比两种不同提取方法对甲壳素纤维结构及性能的影响,并进一步探究提取制备过程中甲壳素的纤维形态及均匀性对其薄膜力学性能、光学透明性的影响。结果表明,与“高温”提取条件相比,经“常温”条件提取所得α-甲壳素纳米纤维具有较高的长径比且尺寸分布均匀,较好地保留了甲壳素原有的天然结构。其中,常温膜的拉伸强度可达(152±2.56) MPa,断裂伸长率约为10.0%,是高温膜的2.50倍左右,具有较好的柔韧性。同时,常温膜还具备优异的光学及热稳定性能,透光率高达91.0%,雾度低至3.19%,在200℃高温条件下仍能保持性状稳定。综上,“常温”条件下提取的甲壳素纳米纤维由于较好地保留了其天然结构优势,使得制备所得膜材料具备优异的力学强度及透光性等。本工作的研究成果为制备全生物质高强度、低雾度柔性透明膜材料提供了理论依据和技术支持,有望作为柔性透明基材应用于柔性电子元件、电子皮肤、智能标签及柔性传感等研究领域。

关键词：甲壳素纳米纤维温和条件高透明度低雾度高强度

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木基柔性电子复合膜的制备及其在传感器中的应用

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复合材料学报 2022年第8期39卷 4057-4064页

作者：王怡仁周彤王露臻吴启静李大纲金永灿陈楚楚南京林业大学材料科学与工程学院南京210037 南京林业大学轻工与食品学院南京210037

为拓展木材多功能化及高值化应用,以轻木为原料,通过脱基质工艺去除木材基质,随后干燥处理获得轻木骨架(WF),进一步乙酰化改性后干燥可制得乙酰化轻木骨架(AWF)。利用浸渍法,将聚二甲基硅氧烷(PDMS)弹性体分别引入WF与AWF制备木基复合... 详细信息

为拓展木材多功能化及高值化应用,以轻木为原料,通过脱基质工艺去除木材基质,随后干燥处理获得轻木骨架(WF),进一步乙酰化改性后干燥可制得乙酰化轻木骨架(AWF)。利用浸渍法,将聚二甲基硅氧烷(PDMS)弹性体分别引入WF与AWF制备木基复合薄膜材料(PDMS/WF与PDMS/AWF)。系统性研究乙酰化改性对复合膜形貌特征、力学性能、化学组分及表面亲水性等的影响。结果表明,乙酰化处理能够有效促进PDMS分布于轻木骨架的孔隙结构中,二者结合紧密,界面结合性提高。同时,制得的PDMS/AWF木基复合膜具备较好的柔韧性,沿纤维生长方向断裂拉伸强度达176 MPa,韧性约6.58 MJ/m^(3),几乎是PDMS/WF复合膜的两倍。进一步将该PDMS/AWF木基复合膜作为柔性基材,组装柔性传感器件,在多种形变下表现出稳定及可重复的相对电阻值变化。预期在智能家居、软体机器人及柔性可穿戴电子等智能响应器件研究领域具有潜在的应用。

关键词：木材聚二甲基硅氧烷乙酰化复合膜柔性电子基材传感器

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基于心智模型的学龄前儿童建构玩具设计研究

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艺术与设计（理论版） 2023年第1期 125-128页

作者：刘宝顺武琪婧天津商业大学天津300134 河北工业大学建筑与艺术设计学院天津300401

文章针对如何提升儿童建构玩具体验满意度的问题,借助心智模型理论挖掘学龄前儿童的使用需求,通过访谈和POEMS观察法收集学龄前儿童使用建构玩具时的行为和态度,根据描述内容的相关性整理出原子任务、任务层、任务塔以及心智空间,并形... 详细信息

文章针对如何提升儿童建构玩具体验满意度的问题,借助心智模型理论挖掘学龄前儿童的使用需求,通过访谈和POEMS观察法收集学龄前儿童使用建构玩具时的行为和态度,根据描述内容的相关性整理出原子任务、任务层、任务塔以及心智空间,并形成学龄前儿童使用建构玩具的心智模型。通过对儿童心智模型需求的分析以及设计机会点的提炼,归纳整合设计方向,使建构玩具满足学龄前儿童身心发展特征和体验需求,为儿童建构玩具的设计提供一定的借鉴。

关键词：心智模型学龄前儿童用户需求建构玩具

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