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Acta Oceanologica Sinica 2013年第11期32卷 41-49页

作者： LI Jiangnan YANG Chaofeng LI Fangzhou he qihua LI Weibiao School of Environmental Sciences and Engineering Sun Yat-sen University Zhejiang Sub-bureau of East China Regional Air Traffic Management Bureau of CAAC

The three-dimensional structures of summer precipitation over the South China Sea （SCS） and the East China Sea （ECS） are investigated based on tropical rainfall measurement mission （TRMM）. The primary results are as follows. First, both the convective and stratiform precipitation rates in the SCS are much higher than those of the ECS. The contribution of the convective cloud precipitation to the surface precipitation is primarily over the SCS and the ECS with a proportion of about 70%, but the contribution of convective cloud precipitation is slightly larger in the SCS than the ECS. The contribution of stratus precipitation is slightly larger in the ECS than that in the SCS. Second, the content of cloud particles and precipitation particles in the ECS in June was greater than that in the SCS, while in July and August, the content of cloud and precipitation particles in the ECS was less than that in the SCS. Third, the latent heat profile of the ECS is quite different from that of the SCS. In June, the peak values of evaporation and condensation latent heating rates in the ECS are greater than those in the SCS. In July and August, however, the peak values of evaporation and condensation latent heating rates in the ECS are about 0.05°/h less than those in the SCS.

关键词： precipitation three-dimensional structures tropical rainfall measurement mission SouthChina Sea East China Sea

Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice

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Chinese Science Bulletin 2000年第1期45卷 49-51页

作者： WANG Xiaoying XING Hong he qihua XU Jialing WU Benjie Department of Biophysics Beijing Medical UniversityBeijing 100083China Analytic Center of Medico-Pharmacy Beijing Medical UniversityBeijing 100083China

The level of lipid peroxidation and cellular necrosis in rat living brain slices during brain ischemia-hypoxia injury have been observed using a laser confocal scanning microscope (LCSM) with double labeling of fluorescent probes D-399 (2, 7-dichlorofluorescin diacetate) and propidium iodide (Pl). The hypoxia and/or reoxygenation injury in rat brain slices is markedly decreased by pretreatment with L-NG-nitro-arginine (L-NNA) and N-acetylcysteine (NAC), showing that the nitric oxide (NO) and other free radicals play an important role in brain ischemia-hypoxia injury.

关键词： laser confocal scanning microscope brain ischemia-hypoxia injury lipid peroxide 2 7-dichIorofluorescin diacetate.

Polarity-activated super-resolution imaging probe for the formation and morphology of amyloid fibrils

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Nano Research 2020年第9期13卷 2556-2563页

作者： Zheng Lv Li Li Zhongwei Man Zhenzhen Xu Hongtu Cui Rui Zhan qihua he Lemin Zheng Hongbing Fu Beijing Key Laboratory for Optical Materials and Photonic Devices Capital Normal UniversityBeijing 100048China Tianjin Key L aboratory of Molecular Optoelectronic Sciences Institute of Molecular PlusTianjin UniversityTianjin 300072China 3 The Institute of Cardiovascular Sciences and Institute of Systems BiomedicineSchool of Basic Medical SciencesKey Laboratory of Molecular Cardiovascular Sciences of Ministry of Education NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory PeptidesHealth Science CenterPeking UniversityBeijing 100191China China National Clinical Research Center for Neurological Diseases Tiantan HospitalAdvanced Innovation Center for Human Brain ProtectionThe Capital Medical UniversityBeijing 100050China

The formation of amyloid plaques usually occurs in the early-stage of Alzheimer’s disease(AD).Stimulated emission depletion(STED)imaging provided a powerful tool for visualizing amyloid structures on the nanometer ***,many commercial probes adopted in detecting amyloid fibrils are inapplicable to STED imaging,owing to their unmatched absorption and emission wavelengths,small Stokes'shift,easy photo-bleaching,***,we demonstrated a polarity-activated STED probe based on an intramolecular charge transfer donor(D)-7c-acceptor(A)*** electron-rich carbazole group and the electron-poor pyridinium bromide group,linked by 7i-conjugated thiophen-bridge,ensure strong near infrared(NIR)emission with a Stokes'shift larger than 200 *** tiny change in polarity before and after binding with amyloid plaques leads to a transition from weakly emission charge-transfer(CT)state(Φhe super-resolution imaging of the formation and morphology of amyloid fibrils in vitro based on this probe was realized with a lateral spatial resolution better than 33 nm at an extremely low depletion ***,the ex-vivo super-resolution imaging of(E)-1-butyl-4(2-(5-(9-ethyl-9Hcarbazol-3-yl)thiophen-2-yl)vinyl)pyridinium bromide(CTPB)probe in Aβ plaques in the brain slices of a Tg mouse was *** research provides a demonstration of the super resolution imaging probe of amyloid fibrils based on polarity-response mechanism,providing a new approach to the development of future amyloid probes.

关键词： polarity-activated amyloid fibrils super-resolution imaging charge transfer near infrared(NIR)emission

Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy

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Molecular Plant 2016年第9期9卷 1229-1239页

作者： Xiaojuan Li Jingjing Xing Zongbo Qiu qihua he Jinxing Lin College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 10049 China College of Life Sciences Henan Normal University Xinxiang 453007 China The health Science Center Peking University Beijing 100191 China These authors contributed equally to this article.

Deciphering the dynamics of protein and lipid molecules on appropriate spatial and temporal scales may shed light on protein function and membrane organization. However, traditional bulk approaches cannot unambiguously quantify the extremely diverse mobility and interactions of proteins in living cells. Fluores- cence correlation spectroscopy （FCS） is a powerful technique to describe events that occur at the singlemolecule level and on the nanosecond to second timescales; therefore, FCS can provide data on the heterogeneous organization of membrane systems. FCS can also be combined with other microscopy techniques, such as super-resolution techniques. More importantly, FCS is minimally invasive, which makes it an ideal approach to detect the heterogeneous distribution and dynamics of key proteins during development. In this review, we give a brief introduction about the development of FCS and summarize the significant contributions of FCS in understanding the organization of plant cell membranes and the dy- namics and interactions of membrane proteins .We also discuss the potential applications of this technique in plant biology.

关键词： fluorescence correlation spectroscopy microdomain membrane protein dynamics interaction

横断山区干旱河谷川滇蔷薇种子休眠与萌发的地理空间差异

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生态学报 2020年第17期40卷 6037-6045页

作者：周志琼何其华中国科学院成都生物研究所中国科学院山地生态恢复与生物资源利用重点实验室生态恢复与生物多样性保育四川省重点实验室成都610041

通过测定横断山区干旱河谷18个川滇蔷薇种群新采集种子以及低温层积8周种子的发芽率和发芽速度,分析发芽率和发芽速度与种子特征以及环境因子之间的关系,阐明川滇蔷薇种子休眠与萌发的地理空间差异及其影响因素。结果表明,18个川滇蔷薇... 详细信息

通过测定横断山区干旱河谷18个川滇蔷薇种群新采集种子以及低温层积8周种子的发芽率和发芽速度,分析发芽率和发芽速度与种子特征以及环境因子之间的关系,阐明川滇蔷薇种子休眠与萌发的地理空间差异及其影响因素。结果表明,18个川滇蔷薇种群种子具有不同程度的休眠,新采集种子发芽率变化幅度大,为15.8%±5.0%至82.7%±2.3%,发芽速度指数范围:2.3%±0.2%至5.3%±0.5%。不同种群种子发芽率和发芽速度差异显著。新采集种子的发芽率在流域间存在显著差异,表现为金沙江流域>雅砻江流域>大渡河流域和岷江流域。新采集种子的发芽率和发芽速度随着采集地点海拔的升高而显著增加,即种子休眠程度随着海拔的升高呈现降低趋势。低温层积8周显著提高了种子发芽率和发芽速度,但减弱了种子发芽率和发芽速度在流域以及海拔间的差异。偏相关分析表明:瘦果皮厚度与新采集种子萌发速度成显著负相关关系;环境因子中年蒸发量与发芽率以及发芽速度之间关系最为密切,成极显著正相关关系;其次为年降水量,与发芽率和发芽速度之间具有显著的负相关关系。综合分析表明,川滇蔷薇种子休眠与萌发在横断山区干旱河谷存在较强地理空间差异。环境因子中年蒸发量和年降水量以及植物自身特征瘦果皮厚度是引起种子休眠与萌发地理空间差异的主要因素。

关键词：川滇蔷薇地理空间差异干旱河谷种子休眠

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电针曲池穴治疗原发性高血压临床研究

亚太传统医药 2018年第5期14卷 166-167页

作者：何琪华西华县人民医院河南周口466600

目的:研究电针曲池穴治疗原发性高血压的临床疗效。方法:将92例原发性高血压患者随机分为对照组和实验组各46例,对照组患者采用常规西药治疗,实验组患者在此基础上加用电针曲池穴疗法,比较两组患者的临床疗效。结果:经过治疗,实验组患... 详细信息

目的:研究电针曲池穴治疗原发性高血压的临床疗效。方法:将92例原发性高血压患者随机分为对照组和实验组各46例,对照组患者采用常规西药治疗,实验组患者在此基础上加用电针曲池穴疗法,比较两组患者的临床疗效。结果:经过治疗,实验组患者总有效率(95.65%)显著高于对照组(78.26%),差异具有统计学意义(P<0.05);实验组患者治疗后收缩压、舒张压水平均显著低于对照组,差异具有统计学意义(P<0.05);实验组患者治疗后血浆NPY含量显著低于对照组,NT含量显著高于对照组,差异具有统计学意义(P<0.05);两组患者治疗期间均未有严重副反应。结论:电针曲池穴治疗原发性高血压效果显著,可明显降低血压,改善血浆NPY、NT含量,且安全性高,具有临床应用价值。

关键词：原发性高血压电针曲池穴

微小RNA-24对骨肉瘤细胞U2OS体外增殖与迁移能力的影响及作用机制

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国际肿瘤学杂志 2017年第7期44卷 490-495页

作者：贺启华陕西省汉中市三二0一医院肿瘤内三科 723000

目的探讨微小RNA-24（miR-24）对人骨肉瘤细胞株U2OS体外增殖与迁移能力的影响及其可能的机制.方法通过转染miR-24模拟物（miR-24 mimic）建立miR-24过表达的U2OS细胞株,采用实时荧光定量逆转录聚合酶链反应（qRT-PCR）、细胞活力检... 详细信息

目的探讨微小RNA-24（miR-24）对人骨肉瘤细胞株U2OS体外增殖与迁移能力的影响及其可能的机制.方法通过转染miR-24模拟物（miR-24 mimic）建立miR-24过表达的U2OS细胞株,采用实时荧光定量逆转录聚合酶链反应（qRT-PCR）、细胞活力检测方法（CCK-8法）和Transwell检测对照组、阴性对照组和miR-24过表达组3组细胞增殖及迁移能力的变化;Western blotting检测细胞的上皮间质转化（EMT）进程及核转录因子-κB（NF-κB）信号通路的活性.结果对照组、阴性对照组和miR-24过表达组miR-24的表达水平分别为1.00±0.00、1.03±0.08和2.46±0.29,3组间差异有统计学意义（F=11.026,P=0.012）,转染了miR-24 mimic后,人骨肉瘤细胞株U2OS中的miR-24表达水平与对照组相比显著升高（t=4.604,P=0.009）.与对照组相比,人骨肉瘤细胞株U2OS过表达miR-24 24 h[（3.56±0.27）%∶（8.63±0.79）%]、48 h[（20.16±1.09）%∶（54.77±5.42）%）]和72 h[（45.47±3.16）%∶（95.52±8.56）%]后细胞增长率均明显降低,差异均有统计学意义（t=3.896,P=0.016;t=4.813,P=0.008;t=7.173,P=0.002）.过表达miR-24 24 h后,对照组、阴性对照组和miR-24过表达组细胞相对迁移率分别为（100.00±0.00）%、（99.26±5.85）%和（31.37±2.09）%,3组间差异有统计学意义（F=12.175,P=0.009）;与对照组相比,过表达miR-24后细胞迁移率明显降低（t=3.843, P=0.014）.同时过表达miR-24上调细胞中上皮细胞标志物上皮细胞钙黏蛋白（E-cadhe-rin,t=3.852,P=0.018）和β-连环素蛋白（β-catenin）的表达（t=3.512,P=0.024）,而下调间质细胞标志物神经钙黏蛋白（N-cadherin,t=3.832,P=0.018）和波形蛋白（vimentin）的表达（t=4.058,P=0.012）,抑制U2OS的EMT进程.此外,过表达miR-24可下调NF-κB（p65）（t=4.813,P=0.008）、磷酸化核因子IκB激酶抑制剂α（p-IKK-α）（t=3.764,P=0.013）和磷酸化激活IκB激酶复合物α（p-IκB-α）的表达（t=4.064,P=0.012）,抑制NF-κB信号通路的激活.结论 miR-24可抑制人骨肉瘤细胞株U2OS体外增殖与迁移,其作用机制可能与抑制NF-κB信号通路的激活,进而抑制细胞的EMT进程相关.提示miR-24可作为治疗骨肉瘤迁移的一个潜在作用位点.

关键词：骨肉瘤细胞运动微小RNA-24

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高层超高层建筑防排烟系统设计

消防技术与产品信息 2011年第2期24卷 17-22页

作者：贺绮华中国建筑设计研究院北京100044

根据某些高层超高层建筑防排烟系统存在的问题,提出改进设计的措施。为确保一旦发生火灾时的迅速扑救及人员的安全疏散,必须切实搞好建筑物内防烟楼梯间、防烟电梯井的加压送风设计和中庭及内走道的机械排烟设计。

关键词：高层超高层建筑防烟楼梯间防烟电梯井加压送风设计机械排烟设计

度洛西汀、西肽普兰对伴躯体疼痛症状的抑郁症患者P物质的影响

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中国健康心理学杂志 2014年第9期22卷 1298-1300页

作者：何其华安徽省安庆市第六人民医院精神科中国246000

目的：观察比较度洛西汀、西肽普兰对伴有躯体疼痛症状的抑郁症患者血浆P物质水平的影响。方法伴有躯体疼痛症状的抑郁症患者随机分组，分别接受度洛西汀（60 mg/d，n＝25），西肽普兰（20～40 mg/d，n＝25）治疗，于治疗前、治疗4、8... 详细信息

目的：观察比较度洛西汀、西肽普兰对伴有躯体疼痛症状的抑郁症患者血浆P物质水平的影响。方法伴有躯体疼痛症状的抑郁症患者随机分组，分别接受度洛西汀（60 mg/d，n＝25），西肽普兰（20～40 mg/d，n＝25）治疗，于治疗前、治疗4、8周末分别测定血浆P物质水平。结果①度洛西汀组治疗4、8周末的血浆P物质水平均较治疗前显著下降（ t＝2．095，3．504；P＝0．046，0．003）；西肽普兰组治疗8周末，血浆P物质水平较治疗前显著下降（ t＝2．170， P＝0．040）；②治疗前、治疗4周末，度洛西汀组、西肽普兰组血浆P物质水平无显著差异；治疗8周末，度洛西汀组血浆P物质水平显著低于西肽普兰组（t＝2．030，P＝0．047）。结论度洛西汀、西肽普兰均可使伴躯体疼痛症状的抑郁症患者血浆SP水平下降，但度洛西汀起效更快、作用更强。

关键词：抑郁症疼痛 P物质度洛西汀西肽普兰

激光扫描共焦显微镜在检测活体组织和细胞中的应用

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中国医学装备 2004年第4期1卷 43-47页

作者：何其华北京大学医药卫生分析中心 100083

激光扫描共焦显微镜(ConfocalLaserScanningMicroscopy,以下简称Confocal)的功能决定了其在生物医学中的应用及独特的地位,它能对完整的活细胞和组织或固定的细胞和组织内各种结构进行定性、定量、定时和定位的测量。但其强大的定位和... 详细信息

激光扫描共焦显微镜(ConfocalLaserScanningMicroscopy,以下简称Confocal)的功能决定了其在生物医学中的应用及独特的地位,它能对完整的活细胞和组织或固定的细胞和组织内各种结构进行定性、定量、定时和定位的测量。但其强大的定位和动态测量功能,使Confocal尤其适合于活体组织和细胞的研究。本文简单介绍一下Confocal的原理和功能,着重阐述了Confocal在活体组织和细胞中的应用。

关键词：激光扫描共焦显微镜活体组织检测 Laser 生物医学动态测量组织内活细胞