Using laser confocal scanning microscope to study ischemia-hypoxia injury in rat brain slice

作     者：WANG Xiaoying XING Hong HE Qihua XU Jialing WU Benjie 

作者机构：Department of BiophysicsBeijing Medical UniversityBeijing 100083China Analytic Center of Medico-PharmacyBeijing Medical UniversityBeijing 100083China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2000年第45卷第1期

页      面：49-51页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

基　　金：Foundation of Chinese Medical Science and Technology  (96-960A-02-21) 

主　　题：laser confocal scanning microscope brain ischemia-hypoxia injury lipid peroxide 2 7-dichIorofluorescin diacetate. 

摘      要：The level of lipid peroxidation and cellular necrosis in rat living brain slices during brain ischemia-hypoxia injury have been observed using a laser confocal scanning microscope (LCSM) with double labeling of fluorescent probes D-399 (2, 7-dichlorofluorescin diacetate) and propidium iodide (Pl). The hypoxia and/or reoxygenation injury in rat brain slices is markedly decreased by pretreatment with L-NG-nitro-arginine (L-NNA) and N-acetylcysteine (NAC), showing that the nitric oxide (NO) and other free radicals play an important role in brain ischemia-hypoxia injury.