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银杏组织培养方案优化研究进展

江苏林业科技 2022年第2期49卷 43-51页

作者：吴奇翰福建师范大学附属中学福建福州350007

构建银杏的植物组织培养技术体系的目的主要有2个,即通过外植体诱导分化愈伤组织,再脱分化,产生不定芽,最终形成完整植株;对愈伤组织进行继代培养和细胞悬浮培养,以提取次生代谢产物。但培养物面临严重的污染和褐化问题,愈伤组织诱导和... 详细信息

构建银杏的植物组织培养技术体系的目的主要有2个,即通过外植体诱导分化愈伤组织,再脱分化,产生不定芽,最终形成完整植株;对愈伤组织进行继代培养和细胞悬浮培养,以提取次生代谢产物。但培养物面临严重的污染和褐化问题,愈伤组织诱导和分化成功率较低,且次生代谢物含量低于天然叶片平均水平,不利于进一步工业化提取应用。该文从外植体类型、取材和保存方法,消毒剂种类、浓度与使用时间,培养基配方、有益化合物添加,外环境条件筛选、pH与温度控制等方面综述了国内外学者对方案作出的优化。针对污染和褐化2大问题,比较各环节的改进做法,讨论为提高成功率和次生代谢物产量进行的改良设计,并为进一步研究提出适宜方案,指出今后的改进方向和研究重点。

关键词：银杏组织培养愈伤组织外植体消毒剂培养基防褐化

Structure Design of Disposable Ocean Temperature Depth Sensing Probe Based on F-P Cavity and FBG

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Journal of Applied Mathematics and Physics 2021年第11期9卷 2947-2953页

作者： Zhaoyue Liu Lina Zeng Ke Xu qihan wu Yichao Shu Xin Liao Zaijin Li Hao Chen Zhongliang Qiao Yi Qu Guojun Liu Lin Li Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province College of Physics and Electronic Engineering Hainan Normal University Haikou China

The basic principle of sensing is the combination of F-P cavity interference and fiber grating reflection. A hybrid structure sensor probe has been designed based on the combination of an F-P cavity of liquid-filled thermometer structure, and a fiber grating with an elastic diaphragm, herein F-P cavity is used for temperature sensing, and the fiber grating is used for pressure sensing. By adopting the dual optical path structure, the dual-parameter detection of temperature and depth is realized, which solves the problem of low accuracy caused by the cross response of temperature and pressure of a single sensor device and the calculation of the depth information of the ocean with empirical formulas. Compared with traditional sensors, the sensitivity is effectively improved. Theoretical analysis shows that the sensitivity of the F-P cavity with a thermometer structure filled with kerosene reaches 1.334 nm/?C, and the depth sensitivity of the fiber grating is 284.6 pm/Mpa within the ocean depth range of 0 - 400 m.

关键词： F-P Cavity Fiber Grating Laser Sensing Liquid Filling

Structure Design of Disposable Ocean Temperature Depth Sensing Probe Based on F-P Cavity and FBG

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Structure Design of Disposable Ocean Temperature Depth Sensi...

基于热解工艺制备烟熏液对西冷牛排风味特征及消费者接受度的影响

The basic principle of sensing is the combination of F-P cavity interference and fiber grating reflection.A hybrid structure sensor probe has been designed based on the combination of an F-P cavity of liquid-filled thermometer structure, and a fiber grating with an elastic diaphragm, herein F-P cavity is used for temperature sensing, and the fiber grating is used for pressure *** adopting the dual optical path structure, the dual-parameter detection of temperature and depth is realized, which solves the problem of low accuracy caused by the cross response of temperature and pressure of a single sensor device and the calculation of the depth information of the ocean with empirical *** with traditional sensors, the sensitivity is effectively *** analysis shows that the sensitivity of the F-P cavity with a thermometer structure filled with kerosene reaches 1.334 nm/℃, and the depth sensitivity of the fiber grating is 284.6 pm/Mpa within the ocean depth range of 0-400 m.

关键词： F-P Cavity Fiber Grating Laser Sensing Liquid Filling

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食品科学 2024年第18期45卷 190-196页

作者：丁浩晗田嘉伟谢祯奇周洁袁佳玟吴棋涵辛星江南大学未来食品科学中心江苏无锡214000

采用热解工艺,在450℃和氮气保护条件下利用桃木制备烟熏液风味剂,研究其对西冷牛排风味特征的影响及消费者接受度。液熏过程采用不同质量分数的烟熏液和腌制时间处理牛排。首先通过焦点小组测试总结描绘牛排感官属性的描述词,并以词云... 详细信息

采用热解工艺,在450℃和氮气保护条件下利用桃木制备烟熏液风味剂,研究其对西冷牛排风味特征的影响及消费者接受度。液熏过程采用不同质量分数的烟熏液和腌制时间处理牛排。首先通过焦点小组测试总结描绘牛排感官属性的描述词,并以词云的形式进行可视化呈现。然后基于Check-All-That-Apply法和主成分分析进行风味评价,结果表明,热解烟熏液大幅提升了牛排关于烟熏风味特征的描述词出现次数,包括“温和的烟熏味”“烧烤味”“熟牛肉香味”“肉香味”“深色的外观”“耐咀嚼”和“爆汁”等。相关性分析结果表明液熏牛排的总体偏好与气味偏好和味道偏好呈正相关。化学性质分析表明本研究中使用的烟熏液浓度相对较低,并未对液熏牛排样品的水分含量、脂肪含量和pH值产生显著影响。

关键词：烟熏风味热解牛排主成分分析

用抑制性差减杂交构建As_2O_3诱导的NB4细胞凋亡相关基因文库

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中国中药杂志 2009年第4期34卷 454-457页

作者：狄春红顾少华谭晓华仙玲玲吴奇涵杨磊石河子大学医学院第一附属医院医学实验室新疆石河子832008 石河子大学新疆地方与民族高发病教育部重点实验室新疆石河子832002 复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室上海200433 华东师范大学生命科学学院上海200062

目的:构建三氧化二砷(As2O3)诱导的急性早幼粒细胞白血病细胞株NB4细胞凋亡相关基因文库。方法:用含4μmol·L-1As2O3和正常培养基培养NB4细胞24h,抽提总RNA,经逆转录酶合成双链cDNA,分别以砷诱导凋亡组和对照组作为tester和driver,进... 详细信息

目的:构建三氧化二砷(As2O3)诱导的急性早幼粒细胞白血病细胞株NB4细胞凋亡相关基因文库。方法:用含4μmol·L-1As2O3和正常培养基培养NB4细胞24h,抽提总RNA,经逆转录酶合成双链cDNA,分别以砷诱导凋亡组和对照组作为tester和driver,进行双向抑制性差减杂交(suppress-ion subtractive hybridization,SSH),筛选As2O3诱导的NB4细胞凋亡相关基因,将差异表达基因进行PCR扩增并与pGEM-Teasy克隆载体连接,转化DH5α大肠杆菌,经蓝白斑筛选获得白色阳性克隆,PCR扩增出未知基因片段。结果:成功构建了分别代表在NB4细胞中表达上调和下调的基因文库。结论:经双向抑制性差减杂交获得了NB4细胞差异表达基因文库,为克隆NB4细胞凋亡相关基因奠定了基础。

关键词：白血病 NB4细胞三氧化二砷抑制性差减杂交凋亡

赋能“双循环”背景下“江海联运”产教联盟的建设思考

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浙江国际海运职业技术学院学报 2022年第1期18卷 27-29页

作者：汪益兵吴其汉付军浙江国际海运职业技术学院浙江舟山316021 浙江新一海海运有限公司浙江舟山316021

在“一带一路”倡议、“长江经济带”发展战略以及交通强国战略等一系列多元组合的机遇背景下,充分发挥产教联盟功能,对构建“双循环”格局具有特别重要意义。文章着重探讨了建设“江海联运”产教联盟的三项工程:校企协同育人工程、社... 详细信息

在“一带一路”倡议、“长江经济带”发展战略以及交通强国战略等一系列多元组合的机遇背景下,充分发挥产教联盟功能,对构建“双循环”格局具有特别重要意义。文章着重探讨了建设“江海联运”产教联盟的三项工程:校企协同育人工程、社会培训及技术服务工程和科技创新提升工程,旨在促进江海联运人才培养、产业发展和技术革新,推动江海联运高质量发展,促进“双循环”格局构建。

关键词：江海联运产教联盟双循环

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腐霉利在番茄上的残留分析

热带农业科学 2013年第11期33卷 51-53页

作者：黄勇平李鹏陈丽君吴啟晗黎霞海南省植保植检站海南海口570100 海南省农药检定管理所海南海口570203

为了评价腐霉利在番茄上的残留状况。指导腐霉利的科学合理使用，本试验选择50％腐霉利可湿性粉剂推荐剂量500．0、750．0g／hm2（有效成分），连续施药2～3次，间隔5d，最后一次施药3、7、10d后采集番茄样品进行残留检测。结果表明，... 详细信息

为了评价腐霉利在番茄上的残留状况。指导腐霉利的科学合理使用，本试验选择50％腐霉利可湿性粉剂推荐剂量500．0、750．0g／hm2（有效成分），连续施药2～3次，间隔5d，最后一次施药3、7、10d后采集番茄样品进行残留检测。结果表明，腐霉利在番茄上的残留量为0．075-0．256mg／kg，小于GB2763—2012规定的农药最高残留量（2mg／kg）：结合番茄成熟期长，建议腐霉利在番茄上的安全间隔期为10d。

关键词：腐霉利番茄最终残留气相色谱

HSV-1 stimulation-related protein HSRG1 inhibits viral gene transcriptional elongation by interacting with Cyclin T2

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Science China(Life Sciences) 2011年第4期54卷 359-365页

作者： wu WenJuan YU Xian LI WeiZhong GUO Lei LIU LongDing WANG LiChun LI qihan Department of Viral Immunology Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical College Kunming 650118 China Department of Dermatology First Affiliated Hospital of Kunming Medical College Kunming 650032 China

The protein encoded by HSRG1(HSV-1 stimulation-related gene 1) is a virally induced protein expressed in HSV-1-infected *** have already reported that HSRG1 is capable of interacting with transcriptional regulator *** further analyze the effects of HSRG1 on the regulation of viral gene transcription,we expressed the HSRG1 protein in transfected cells and found that it postpones the proliferation of ***(chloramphenicol acetyltransferase) assays also revealed that HSRG1 reduces transcription from HSV-1 *** two-hybrid and immunoprecipitation assays indicated that HSRG1 interacts with Cyclin T2,the regulatory subunit of P-TEFb,which is required for transcription elongation by RNA Pol II(RNAP II) ,and that amino acid residues 1-420 in Cyclin T2 are important for binding with *** assays suggested that the cellular localizations of those two proteins are influenced by their *** analyses with CAT assays revealed that HSRG1 inhibits the transcriptional activation by Cyclin T2 of viral *** results suggested that the inhibitory effects of HSRG1 on viral replication and proliferation are probably induced by its binding to Cyclin ***,it is likely that HSRG1 inhibits viral gene transcriptional elongation by interacting with Cyclin T2.

关键词： herpes simplex virus 1 HSRG1 Cyclin T2 transcriptional regulation

A microRNA encoded by HSV-1 inhibits a cellular transcriptional repressor of viral immediate early and early genes

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Science China(Life Sciences) 2013年第4期56卷 373-383页

作者： wu WenJuan GUO ZhongPing ZHANG XueMei GUO Lei LIU LongDing LIAO Yun WANG JingJing WANG LiChun LI qihan Institute of Medical Biology Chinese Academy of Medical Sciences Peking Union Medical College First Affilliated Hospital of Kunming Medical University Chinese Pharmacopoeia Commission

Viral microRNAs are one component of the RNA interference phenomenon generated during viral infection. They were first identified in the Herpesviridae family, where they were found to regulate viral mRNA translation. In addition, prior work has suggested that Kaposi＇s sarcoma-associated herpesvirus （KSHV） is capable of regulating cellular gene transcription by miRNA. We demonstrate that a miRNA, hsvl-mir-H27, encoded within the genome of herpes simplex virus 1 （HSV-1）, targets the mRNA of the cellular transcriptional repressor Kelch-like 24 （KLHL24） that inhibits transcriptional efficiency of viral imme- diate-early and early genes. The viral miRNA is able to block the expression of KLHL24 in cells infected by HSV-1. Our dis- covery reveals an effective viral strategy for evading host cell defenses and supporting the efficient replication and prolifera- tion of HSV- 1.

关键词： herpes simplex virus I (HSV-1) microRNAs (miRNAs) cellular transcriptional repressor Kelch-like 24