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Exosomes derived from mesenchymal stem cells ameliorate early inflammatory responses following traumatic brain injury

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Exosomes derived from mesenchymal stem cells ameliorate earl...

2018年浙江省神经外科学学术年会

作者： Lijie Huang Haoqi Ni qichuan zhuge the First Affiliated Hospital of Wenzhou Medical University

Objective Traumatic brain injury (TBI) is a leading cause of mortality and disability worldwide. Although there are treatment guidelines for TBI, there exists no best treatment option or medicine for this condition. Recently,mesenchymal stem cells (MSCs) derived exosomes have drawn lots of attention in the treatment of brain disorders with some results highlighting its neuroprotective effects through neurogenesis and angiogenesis after TBI. However,

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The Underlying Mechanism of Reprogramming Process by SOX4/11 and NGN2: Reprogramming Glioblastoma Cells into Neuron Cells

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The Underlying Mechanism of Reprogramming Process by SOX4/11...

2018年浙江省神经外科学学术年会

作者： Jianjing Yang Chun-li Zhang qichuan zhuge Department of Neurosurgery The First Affiliated Hospital of Wenzhou Medical University Department of Molecular Biology University of Texas Southwestern Medical Center

Objective Glioblastoma is the most common malignant tumor in the central nervous system. We previously demonstrated that pro-neural transcription factors mouse sox11 (msox11) could catalyze human NGN2 (hNGN2) highly efficient reprogramming glioblastoma cell into neuron cell. However, the underlying mechanism is largely remains unknown and the clinical application is limited.

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PolyacrylicAcid-Coated Nanoparticles for the Treatment of TissurePlasminogen Activator in Stroke

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PolyacrylicAcid-Coated Nanoparticles for the Treatment of Ti...

2014浙江省神经外科学学术年会

作者： Lijie Huang Jixian Wang zhuge qichuan Kunlin Jin Department of Neurosurgery the First Affiliated Hospital of Wenzhou Medical University

Purpose:Nanoparticle basedthrombolysisis a challenged treatment of *** aim of our study is to investigate the efficacy of targeted thrombolysis with recombinant tissue plasminogen activator(rtPA).Methods:The rtPA is covalently bound to magnetic nanoparticle(MNP)and retained to the target site by an external *** acid-coated MNP was synthesized,rtPA was immobilized to PAA-MNP through carbodiimide-mediated amide bound *** in vitro test,the blood clot was formed in PE tubing with anti-coagulatedplasma,fibrin and calcium *** in vivo test,4 groups of the ferric chloride induced distal middle cerebral artery occlusion model was designed and treated with PBS,MNP,rtPA and MNP-rtPA ***:The immobilization efficacy is 80.7±1.5μg rtPA bound to 1mg *** vitro testing with blood clot in PE tube,the mean lysed percent increased rapidly with the MNP and rtPA in the rotated magnetic field,from 1.28%to 77.40%.The lysis efficiency of MNP-rtPA is 27.3±1.3%and increased to 42.8±2.8%with the rotation of magnetic *** mean infarcted areas of the 4 groups are 0.09±6.07mm,18.28±2.69mm,8.65±3.63mm and 4.40±***:the magnetic targeted MNP-rtPA accelerated thrombolysis and is available to reduce infarction area in brain *** approach may achieve feasible and effective treatment for embolic cerebral ischemia.

关键词： tissue plasminogen activator magnetic nanoparticle middle cerebral artery occlusion thrombolysis

The Neurotrophic Effect of Epidermal Growth Factor in Central Nervous System

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The Neurotrophic Effect of Epidermal Growth Factor in Centra...

2009年浙江省神经外科学术年会

作者： Wang xuyang Li xiaoli Zheng weiming zhuge qichuan

Many studies showed that EGF and EGF-R is extensively distributed in the central nervous system for many *** EGF play an important role in the in central nervous *** is implicated in the developmental regulation of various brain *** perfoms its functions in cerebral cortex,cerebellum, hippocampus,substantia nigra,spinal cord,hypothalamus pituitary,et *** the EGF-R is Stimulated by EGF,it produces numerous effects on central nervous system(CNS) cells in vitro and in vovo including neuronal survival and differentiation,astrocyte proliferation and the proliferation of multipotent *** addition EGF affects the developmental *** the involvement of epidermal growth factor in the signaling pathway of other factors had also been *** EGF receptor stimulation given during limited neonatal stages can result in severe and persistent cognitive/behavioral *** using EGF or reconstructive EGF is a hope to cure nerve system disease.

关键词： Epidermal growth factor Epidermal growth factor receptor Neurotrophin therapy Central nervous system injury Central nervous system

Construction of lentiviral vector encoding human vascular endothelial growth factor165 gene and transfection of human neural stem cells

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Construction of lentiviral vector encoding human vascular en...

2014浙江省神经外科学学术年会

作者： Yu Zhang Zhipeng Su Huajun Jing Xiaobing Wang qichuan zhuge Department of Neurosurgery First Affiliated Hospital of Wenzhou Medical University

Background:Despite neural stem cells(NSCs)transplantation can promote function recovery of intracerebral hemorrhage(ICH)rats,low survival rate of grafted NSCs in ICH rats is a grave *** studies show that vascular endothelial growth factor(VEGF)play key roles in proliferation,differentiation and survival of ***:We construct a lentiviral vector carrying VEGF165 gene,which was used to transfect human NSCs,so as to produce therapeutic seed *** amplified VEGF165gene from human breast cancer cell line MCF-7 using RT-PCR and cloned the target gene into the pCDH-CMV-MCS-EF1-copGFP,a lentiviral expressing *** transformation,Enzyme digestion got a correct length of VEGF165 gene,DNA sequencing analysis confirmed that VEGF165 gene sequence was exactly same as that reported by *** then transfected the recombinant lentivirus produced by 293T cells after cotransfection of pCDH-VEGF165 and packaging plasmids into passage fourth human *** efficiency is 63%,tested by flow cytometry(FCM),VEGF165 mRNA and protein stable expression in transfected NSCs(VEGF165-NSCs)from 1 week after infection,and continue to 2 ***:Proliferation and VEGF secretion ability of NSCs significantly increased in pCDH-VEGF165 transfection group compared with empty vector transfected group and bland control ***-VEGF165 transfected NSCs still keep their characteristics and multiplex differentiation ***:Our results indicate that we have constructed the lentiviral vector carrying human VEGF165 gene successfully and accomplished transfection of human NSCs,which may be used as a gene therapeutic tool to promote proliferation,differentiation and survival of NSCs in treatment of ICH.

关键词： VEGF Lentiviral vector Neural stem cell(NSC) Transfection

Conventional craniotomy versus conservative treatment in patients with minor spontaneous intracerebral hemorrhage in the basal ganglia

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Chinese Neurosurgical Journal 2022年第4期8卷 258-267页

作者： Ning Wang Weiwei Lin Xuanhao Zhu Qi Tu Daqian Zhu Shuai Qu Jianjing Yang Linhui Ruan qichuan zhuge Brain Center Affiliated Zhejiang HospitalZhejiang University School of MedicineHangzhou 310000China Department of Neurosurgery Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang University88 Jiefang RoadHangzhou 310009ZhejiangChina Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China Department of Neurosurgery The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000ZhejiangChina

Background:The treatment for spontaneous intracerebral hemorrhage(ICH)is still controversial,especially for hematomas in the basal ganglia.A retrospective case-control study with propensity score matching was performed to compare the outcomes of conventional craniotomy and conservative treatment for patients with minor ICH in the basal ***:We retrospectively collected the data of consecutive patients with minor basal ganglia hemorrhage from January 2018 to August *** compared clinical outcomes of two groups using propensity score *** extended Glasgow outcome scale obtained by phone interviews based on questionnaires at a 12-month follow-up was used as the primary outcome *** to a previous prognosis algorithm,patients were divided into good and poor prognosis groups to obtain a dichotomized(favorable or unfavorable)outcome as the primary *** outcomes included hospitalized complications,mortality,and modified Rankin score at 12 ***:A total of 54 patients were analyzed,and the baseline characteristics of patients in the surgery and conservative treatment groups were well *** primary favorable outcome at 12 months was significantly higher in the conservative treatment group than in the surgery group(81%vs 44%;OR 1.833,95%CI 1.159-2.900;P=0.005).The incidence of pneumonia in the surgery group was significantly higher than that in the conservative treatment group(P=0.005).Conclusions:It is not recommended to undertake conventional craniotomy for patients with a minor hematoma(25-40 ml)in the basal *** open craniotomy might induce worse long-term functional outcomes than the conservative treatment.

关键词： Basal ganglia Conservative treatment Craniotomy Intracerebral hemorrhage

烧伤早期大鼠回肠肌间神经丛内氮能神经和Cajal间质细胞的变化

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解放军医学杂志 2006年第11期31卷 1068-1070页

作者：钱旭林晨诸葛启钏温州温州医学院组织学与胚胎学教研室浙江325023 温州医学院附属第一医院神经外科

目的了解烧伤早期大鼠肠功能障碍时肌间神经丛的形态学改变,并探讨肠功能异常的机制。方法20只大鼠随机分成对照组(n=10)和烧伤组(n=10),建立30%TBSA深Ⅱ度烧伤大鼠模型,测定肠传输速率。用酶组织化学方法观察回肠肌间神经丛内氮能神经... 详细信息

目的了解烧伤早期大鼠肠功能障碍时肌间神经丛的形态学改变,并探讨肠功能异常的机制。方法20只大鼠随机分成对照组(n=10)和烧伤组(n=10),建立30%TBSA深Ⅱ度烧伤大鼠模型,测定肠传输速率。用酶组织化学方法观察回肠肌间神经丛内氮能神经的组织学改变,并对两组大鼠回肠肌间神经丛内一氧化氮合酶(NOS)阳性神经元、神经节和Cajal间质细胞(ICC)的密度进行测定。结果烧伤组大鼠肠传输速率明显延迟,回肠肌间神经丛内NOS阳性神经元和ICC数量均明显减少(P<0·01),同时伴有神经纤维部分断裂,未能形成完整的神经纤维网。结论烧伤大鼠回肠肌间神经丛氮能神经和ICC的改变可能是烧伤引起肠功能障碍的原因。

关键词：烧伤氮能神经肌间神经丛 Cajal间质细胞

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第四脑室肿瘤的显微外科治疗

中华显微外科杂志 1999年第2期22卷 87-88页

作者：诸葛启钏吴近森瞿宣兴林岩崇浙江省温州医学院附属第一医院神经外科

目的研究第四脑室肿瘤显微外科治疗的效果。方法通过显微外科技术切除第四脑室肿瘤３４例，其中手术全切除３１例，近全切除３例。结果脑瘤基底附着第四脑室底１５例，全切除１２例，近全切除３例；肿瘤基底附着脉络丛、第四脑室顶和侧... 详细信息

目的研究第四脑室肿瘤显微外科治疗的效果。方法通过显微外科技术切除第四脑室肿瘤３４例，其中手术全切除３１例，近全切除３例。结果脑瘤基底附着第四脑室底１５例，全切除１２例，近全切除３例；肿瘤基底附着脉络丛、第四脑室顶和侧壁１９例，均全切除。病理：室管膜瘤１４例，髓母细胞瘤８例，星形细胞瘤４例，血管网状细胞瘤３例，脉络丛乳头状瘤２例，脑膜瘤２例，脑脂瘤１例；手术死亡１例。结论脑肿瘤基底附着第四脑室底髓纹以下并向下生长者手术难以全切除。

关键词：脑肿瘤第四脑室肿瘤显微外科手术

Melatonin Enhances the Therapeutic Effect of Plasma Exosomes Against Cerebral Ischemia-Induced Pyroptosis Through the TLR4/NF-kB Pathway

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Melatonin Enhances the Therapeutic Effect of Plasma Exosomes...

2020年浙江省神经外科学学术大会

作者： Kankai Wang Junnan Ru Hengli Zhang Jiayu Chen Xiao Lin Zhongxiao Lin Min Wen Lijie Huang Haoqi Ni qichuan zhuge Su Yang Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research The First Affiliated Hospital of Wenzhou Medical University Department of Neurosurgery The First Affiliated Hospital of Wenzhou Medical University

Introduction: Ischemic stroke-induced inflammation and inflammasome-dependent pyroptotic neural death cause serious neurological ***-sized plasma exosomes have exhibited therapeutic potential against ischemia and reperfusion injury by ameliorating *** enhance its therapeutic potential in patients with ischemic injury,we isolated exosomes from melatonin-treated rat plasma and assessed the neurological protective effect in a rat model of focal cerebral ***: Basal plasma exosomes and melatonin-treated plasma exosomes were isolated and intravenously injected into a rat model of focal cerebral *** recovery was evaluated by determining the modified neurological severity score (mNSS),infarct volume,and brain water *** in the ischemic cortex was detected through dUTP nick-end labeling (TUNEL) assay,lactate dehydrogenase (LDH) release,and gasdermin D (GSDMD) ***3 inflammasome assembly and global inflammatory cytokine secretion were detected by enzyme-linked immunosorbent assay (ELISA) and Western blot *** immunized Sprague–Dawley rats,microglia pyroptosis was determined through a positive percentage of IBA1+ and caspase-1 (p20)+ ***,the microRNA (miRNA) profiles in melatonin-treated plasma exosomes were analyzed by exosome miRNA microarray ***: Melatonin treatment enhanced plasma exosome therapeutic effects against ischemia-induced inflammatory responses and inflammasome-mediated *** addition,we confirmed that ischemic stroke-induced pyroptotic cell death occurred in the microglia and neuron,while the administration of melatonin-treated exosomes further effectively decreased the infarct volume and improved recovery of function via regulation of the TLR4/NF-κB signaling ***,the altered miRNA profiles in the melatonin-treated plasma exosomes demonstrated the regulatory mechanisms involved in neurological recovery after ischemic ***: This s

关键词： ischemic stroke,melatonin plasma exosome pyroptosis inflammatory response TLR4/NFkB signaling pathway