PolyacrylicAcid-Coated Nanoparticles for the Treatment of TissurePlasminogen Activator in Stroke
作者单位：Department of Neurosurgerythe First Affiliated Hospital of Wenzhou Medical University
会议名称：《2014浙江省神经外科学学术年会》
会议日期：2014年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
关 键 词：tissue plasminogen activator magnetic nanoparticle middle cerebral artery occlusion thrombolysis
摘 要：Purpose:Nanoparticle basedthrombolysisis a challenged treatment of *** aim of our study is to investigate the efficacy of targeted thrombolysis with recombinant tissue plasminogen activator(rtPA).Methods:The rtPA is covalently bound to magnetic nanoparticle(MNP)and retained to the target site by an external *** acid-coated MNP was synthesized,rtPA was immobilized to PAA-MNP through carbodiimide-mediated amide bound *** in vitro test,the blood clot was formed in PE tubing with anti-coagulatedplasma,fibrin and calcium *** in vivo test,4 groups of the ferric chloride induced distal middle cerebral artery occlusion model was designed and treated with PBS,MNP,rtPA and MNP-rtPA ***:The immobilization efficacy is 80.7±1.5μg rtPA bound to 1mg *** vitro testing with blood clot in PE tube,the mean lysed percent increased rapidly with the MNP and rtPA in the rotated magnetic field,from 1.28%to 77.40%.The lysis efficiency of MNP-rtPA is 27.3±1.3%and increased to 42.8±2.8%with the rotation of magnetic *** mean infarcted areas of the 4 groups are 0.09±6.07mm,18.28±2.69mm,8.65±3.63mm and 4.40±***:the magnetic targeted MNP-rtPA accelerated thrombolysis and is available to reduce infarction area in brain *** approach may achieve feasible and effective treatment for embolic cerebral ischemia.