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Co-expression of calretinin and parvalbumin in the rat facial nucleus

Neural Regeneration Research 2008年第9期3卷 991-994页

作者： qiben wang Linfeng Zheng Qinghong Huang Yanbin Meng Manyuan Kuang Department of Human Anatomy Xiangnan College Chenzhou 423000 Hunan Province China Department of Neurobiology and Anatomy Central South University Changsha 410013 Hunan Province China

BACKGROUND： Calretinin and parvalbumin are members of the intracellular calcium binding protein family, which transform Ca^2＋ bioinformation into regulation of neuronal and neural network activities. OBJECTIVE： To observe expression and co-expression of calretinin and parvalbumin in rat facial nucleus neurons . DESIGN, TIME AND SETTING： Neuronal morphology experiment was performed at the Research Laboratory of Applied Anatomy, Department Neurobiology and Anatomy, Xiangya Medical College of Central South University from August to October 2007. MATERIALS： Five healthy, adult Sprague Dawley rats were selected. Polyclonal rabbit-anti-parvalbumin and mouse-anti-calretinin were provided by Sigma, USA. METHODS： Rat brains were obtained and cut into coronal slices using a freezing microtome. Slices from the experimental group were immunofluorescent stained with polyclonal rabbit-anti-parvalbumin and mouse-anti-calretinin antibodies. The control group sections were stained with normal rabbit and mouse sera. MAIN OUTCOME MEASURES： Immunofluorescent double-staining was used to detect calretinin and parvalbumin expression. Nissl staining was utilized for facial nucleus localization and neuronal morphology analysis. RESULTS： The majority of facial motor neurons was polygon-shaped, and expressed calretinin and parvalbumin. The calretinin-immunopositive neurons also exhibited parvalbumin immunoreactivity, that is, calretinin and parvalbumin were co-expressed in the same neuron. CONCLUSION： Calretinin and parvalbumin were expressed in facial nucleus neurons, with varied distribution.

关键词： calretinin facial nucleus parvalbumin rats

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Spatiotemporal expression of inducible nitric oxide synthase and cyclooxygenase 2 in the spinal cord during early stage sciatic nerve crush injury

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Neural Regeneration Research 2010年第10期5卷 747-751页

作者： qiben wang Linfeng Zheng Yinggui Xie Qinghong Huang He Huang Zhicheng Zeng Song Wu Department of Human Anatomy XiangNan University Chenzhou 423000 Hunan Province China Department of Radiology Shanghai First People's Hospital Medical College Shanghai Jiaotong University Shanghai 200080 China Department of Orthopaedics Third Xiangya Hospital Central South University Changsha 410013 Hunan Province China Department of Human Anatomy and Neurobiology Xiangya School of Medicine Central South University Changsha 410013 Hunan Province China

BACKGROUND： Previous studies have shown that inducible nitric oxide synthase （iNOS） and cyclooxygenase 2 （COX-2） participate in inflammatory immune responses and neuropathic pain following peripheral nerve injury. However, few reports have addressed time-dependent expression of iNOS and COX-2 following peripheral nerve injury. OBJECTIVE： To investigate spatiotemporal expression of iNOS and COX-2 during early stage sciatic nerve crush ***, TIME AND SETTING： The randomized, controlled, animal experiment was performed at the Laboratory of Applied Anatomy, Department of Human Anatomy and Neurobiology, Central South University, China from September 2006 to September ***： Mouse anti-rat iNOS monoclonal antibody and goat anti-rat COX-2 monoclonal antibody （Transduction Laboratory, USA）, as well as biotinylated rabbit anti-mouse lgG and biotinylated rabbit anti-goat IgG （Santa Cruz Biotechnology, USA） were used in the present ***： A total of 48 healthy, adult, Sprague Dawley rats were randomly assigned to three groups. In the model group （n = 32）, crush injury to the right sciatic nerve was established using an artery clamp. The model group was further assigned to four subgroups according to survival time （6,12, 24, and 72 hours）, respectively （n = 8）. Sham surgery （n = 8） and normal control （n = 8） groups were also *** OUTCOME MEASURES： iNOS and COX-2 expression was detected in the L4-6 spinal cord with immunohistochemistry. Gray values of iNOS- and COX-2-postive cells in the anterior horn and posterior horn of spinal cord, as well as quantification of iNOS- and COX-2-positive cells in the anterior horn of spinal cord, were ***： iNOS and COX-2 expression gradually increased in the anterior horn and posterior horn of the spinal cord on the damaged side over time from 6 hours following sciatic nerve injury （P〈0.05） and peaked at 72 hours. Simultaneously, the number of iNOS- and COX-2-positive cells similarly increased

关键词： inducible nitric oxide synthase cyclooxygenase 2 sciatic nerve spinal cord peripheral nerve injury neural regeneration

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Alteration of P2X3 expression in dorsal root ganglia after sciatic nerve ligation

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Neural Regeneration Research 2007年第4期2卷 198-202页

作者： Guoxing Zhou Lesi Xie qiben wang Qingping Yu Xiaofu Liu Qiumei Liu Wei Peng Zhicheng Zeng Department of Morphology Hunan Traditional Chinese Medical College Zhuzhou 412012 Hunan Province China Department of Human Anatomy and Neurobiology Xiangya Medical College Central South University Changsha 410013 Hunan Province China Department of Human Anatomy Xiangnan College Chenzhou 423000 Hunan Province China

BACKGROUND： The expressions of P2X3 receptor in dorsal root ganglia （DRG） after different peripheral nerve injuries are diverse. It indicates the different roles of P2X3 in different models-caused neuropathologic pains. OBJECTIVE： To observe the expressions of P2X3 in corresponding DRG after sciatic nerve ligation in rots. DESIGN： Controlled observation experiment. SETTING： Department of Morphology, Hunan Traditional Chinese Medical College; Department of Human Anatomy and Neurobiology, Xiangya Medical College, Central South University. MATERIALS： Thirty-five healthy adult SD rots of clean grade an d either gender, weighing （ 200 ± 20 ） g, were involved. According to the random digits table, the involved rats were randomized into 3 groups： normal group （n =5）, sham-operated group （n =5） and experimental group （n =25）. The experimental group were subdivided into 3, 7, 14, 21, 28 days groups according to different surviving time after operation, 5 rots at each time point. Polyclonal rabbit anti-P2X3 antibody （ABCAM company）; biotinylated goat anti-rabbit IgG （Zhongshanjingqiao Biotechnical Co., Ltd., Beijing）; Motic fluorescence microscope （Motic, Germany）. METHODS： The experiments were carried out in the Department of Human Anatomy and Neurobiology, Xiangya Medical College, Central South University from June to December 2006. ① Rats of experimental group were created into models by ligation of right sciatic nerve according to the method of Seltzer et al. Left sciatic nerve was used as self-control. As for rats in the sham-operated group, ligation of sciatic nerve was omitted, but other procedures were the same as those in the experimental group. Rats of normal group were untouched, ② Rats of the normal group and sham-operated group survived for 14 days separately, and those of experimental group survived for corresponding time. After being deeply anesthetized by intraperitoneal injection of over-dose sodium pentobarbital, the rots of experimental group were t

关键词： sciatic nerve ligation ganglia neuralgia

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间羧基偶氮羧与钯的显色反应

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科技创新导报 2021年第6期18卷 58-60页

作者：王其本连云港晴好工程咨询有限公司江苏连云港222005

本文研究了在微乳Tween-80存在下,Pd(Ⅱ)与间羧基偶氮羧(m-CCA)的显色反应,试验表明,在pH 3.5的醋酸-醋酸钠(HAc-NaAc)溶液中,Pd(Ⅱ)与试剂形成1﹕2的紫红色配合物,630 nm波长处为配合物的最大吸收峰,表观摩尔吸光系数为2.68×104 L·mo... 详细信息

本文研究了在微乳Tween-80存在下,Pd(Ⅱ)与间羧基偶氮羧(m-CCA)的显色反应,试验表明,在pH 3.5的醋酸-醋酸钠(HAc-NaAc)溶液中,Pd(Ⅱ)与试剂形成1﹕2的紫红色配合物,630 nm波长处为配合物的最大吸收峰,表观摩尔吸光系数为2.68×104 L·mol^(-1)·cm^(-1)。Pd(II)的含量经测量在0~15 g/10 mL范围内,符合比尔定律。本法用于碳钯催化剂中钯的测定,获得了较为成功的结果。

关键词：间羧基偶氮羧显色反应分光光度法钯

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基于GPS无线定位/GSM短信收发的空间信息查询系统

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微计算机信息 2006年第2X期22卷 184-186页

作者：王婍犇刘锦高上海华东师范大学电子系 200062

空间信息查询系统集GPS、GIS和GSM通信于一体,实现移动用户定位和空间信息查询等系统功能。本文主要侧重于介绍移动用户终端软、硬件方面的功能实现。硬件方面,利用单片机完成系统控制,通过串口扩展芯片连接GPS全球定位、GSM移动通信,以... 详细信息

空间信息查询系统集GPS、GIS和GSM通信于一体,实现移动用户定位和空间信息查询等系统功能。本文主要侧重于介绍移动用户终端软、硬件方面的功能实现。硬件方面,利用单片机完成系统控制,通过串口扩展芯片连接GPS全球定位、GSM移动通信,以及LCD汉显三个模块。软件方面主要实现地理信息的实时处理,信息的人性化显示以及与短信服务中心的通信。

关键词：全球定位模块移动通信模块汉现LCD模块串口扩展

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血清miRNA-93、miRNA-223联合miRNA-324-3p可作为诊断PCOS的生物标记物

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基因组学与应用生物学 2019年第2期38卷 887-892页

作者：张伟欧秋娟王岐本张天杰唐伟军湘南学院生理学教研室郴州423000 湘南学院附属医院郴州423000 湘南学院教务处郴州423000

本研究拟基于最近的miRNA报道,结合前期关于肌肉生长抑素(myostatin, MSTN)的相关研究,探究多囊卵巢综合症(ploycystic ovary syndrome, PCOS)患者血清miRNA和生长抑素的表达及其相关临床病理特征。本研究选取160例在湘南学院附属医院... 详细信息

本研究拟基于最近的miRNA报道,结合前期关于肌肉生长抑素(myostatin, MSTN)的相关研究,探究多囊卵巢综合症(ploycystic ovary syndrome, PCOS)患者血清miRNA和生长抑素的表达及其相关临床病理特征。本研究选取160例在湘南学院附属医院妇科就诊的女性为研究对象,通过PCR、ELISA等方法比较PCOS患者血清miRNA和生长抑素的表达情况,并且分析其相关临床病理特征。结果显示,通过PCR的方法发现mi RNA-93、mi RNA-223在PCOS患者的血清中显著升高,而mi RNA-4522、mi RNA-6767-5p和mi RNA-324-3p则表达下降。本研究建立了3个miRNA的预测模型,并证实预测模型在筛选组和验证组中有很好的敏感性和特异性,可以有效区分PCOS患者和正常人群,但研究结果也发现,miRNA模型结合MSTN没有更好的诊断价值。

关键词：多囊卵巢综合症血清miRNA 肌肉生长抑素生物标记物

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大鼠坐骨神经结扎后脊髓内诱导型一氧化氮合酶的表达

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解剖学杂志 2008年第5期31卷 687-690页

作者：王岐本余清平谢乐斯黄庆红潘爱华湘南学院解剖学教研室郴州423000 中南大学解剖学与神经生物学系长沙410013

目的：研究坐骨神经结扎损伤后诱导型一氧化氮合酶（iNOS）在大鼠脊髓内的表达变化规律。方法：健康成年SD大鼠随机分为正常组、假手术组和坐骨神经结扎组,存活1、3、5、7、14、21、28 d后取腰4～6脊髓节段冷冻切片,用免疫组织化学方法... 详细信息

目的：研究坐骨神经结扎损伤后诱导型一氧化氮合酶（iNOS）在大鼠脊髓内的表达变化规律。方法：健康成年SD大鼠随机分为正常组、假手术组和坐骨神经结扎组,存活1、3、5、7、14、21、28 d后取腰4～6脊髓节段冷冻切片,用免疫组织化学方法结合图像分析技术检测脊髓内i NOS的表达变化,同时用免疫荧光双标染色技术检测14 d组中央管周围iNOS和神经肽Y（NPY）的共表达。结果：根据免疫阳性产物灰度值,损伤侧脊髓前角iNOS表达1 d明显升高,21 d达高峰,28 d接近正常,损伤后1～21 d损伤侧脊髓前角与对侧和正常组免疫阳性产物相比有统计学意义;损伤侧脊髓后角1 d iNOS表达增强,其他各组未见明显变化;损伤后中央管周围免疫阳性细胞数增多,3 d尤为明显,28 d恢复正常,免疫荧光双标染色显示14 d组i NOS和NPY在中央管周围共表达。结论：iNOS可能参与神经损伤后的再生过程及伤后神经性痛的调节。

关键词：诱导型一氧化氮合酶神经肽Y 坐骨神经损伤脊髓大鼠

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医患交流技能学课程提高临床医学生医患沟通能力的实践研究

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科教导刊 2024年第14期 149-151页

作者：汤银娟王岐本韦雅光湘南学院湖南郴州423000

目的:探讨医患交流技能学课程对提高临床医学生医患沟通能力的效果,为培养临床医学生医患沟通能力的医学教育提供依据。方法:采用彭红编著的《医学生医患沟通能力量表》了解医学生开课前和结课后的医患沟通能力,结课后评价课程的特点及... 详细信息

目的:探讨医患交流技能学课程对提高临床医学生医患沟通能力的效果,为培养临床医学生医患沟通能力的医学教育提供依据。方法:采用彭红编著的《医学生医患沟通能力量表》了解医学生开课前和结课后的医患沟通能力,结课后评价课程的特点及应用。结果:2018级和2019级医学生的医患沟通能力显著提高,多数医学生认为,教程内容丰富,版面设计合理,实用性较好,建议活泼语言形式,加入最新进度和评估体系。结论:开设医患交流技能学教程,可明显提高医学生的医患沟通能力,地方高校可借鉴该方式来培养临床医学生的医患沟通能力。

关键词：临床医学生医患沟通能力交流技能学

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