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aBIOTECH 2021年第4期2卷 343-356页

作者： Guotang Yang Qi Zheng Pan Hu Hongwei Li qiaoling luo Bin Li Zhensheng Li State Key Laboratory of Plant Cell and Chromosome Enginering Institute of Genetics and Developmental Biology The Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Stripe rust,caused by Puccinia striformis ***(Pst),is one of the most destructive diseases of wheat(Triticum aestivum L)*** 78829,a partial amphidiploid developed by crossing common wheat with Thinopyrum intermedium,is immune to wheat stripe *** transfer the resis-tance gene of this excellent germplasm resource to wheat,the tr anslocation line WTT11 was produced by pollen irradiation and assessed for immunity to stripe rust races CYR32,CYR33 and CYR34.A novel stripe rust-resistance locus derived from *** was confirmed by linkage and diagnostic marker *** cytogenetic analyses revealed that WTT11 carries a TTh 2DL *** breakpoint of 1B was located at 95.5 MB,and the alien segments were found to be homoeologous to wheat-group chromosomes 6 and 7 according to a wheat660K single-nucleotide polymorphism(SNP)array *** previously developed PCR-based markers were confirmed to rapidly trace the alien segments of WTT11,and 20 kompetitive allele-specific PCR(KASP)markers were developed to enable genotyping of *** and common *** of agronomic traits in two consecutive crop seasons uncovered some favorable agronomic traits in WTT11,such as lower plant height and longer main panicles,that may be applicable to wheat *** a novel genetic resource,the new resistance locus may be useful for wheat disease-resistance breeding.

关键词： Wheat-Thinopyrum intermedium translocation line CYR34 Cytogenetic analyses Wheat660k SNP array PCR-based markers KASP markers

Transcriptome analysis of salt-stress response in three seedling tissues of common wheat

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The Crop Journal 2019年第3期7卷 378-392页

作者： qiaoling luo Wan Teng Shuang Fang Hongwei Li Bin Li Jinfang Chu Zhensheng Li Qi Zheng State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China National Centre for Plant Gene Research (Beijing) Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Xiaoyan 60(XY60) is a new wheat variety bred in the laboratory of Zhensheng *** salt treatment, seedlings of XY60 maintain green leaves and produce longer roots than the high yielding cultivar Zhongmai 175(ZM175).To explain these different phenotypes we carried out an RNA-Seq analysis using 12 samples from three tissues of both varieties subjected to salt and control *** comparing data from the salt treated plants with the control, 703, 979, and 1197 differentially expressed genes(DEGs) were detected in new leaves, old leaves, and roots of XY60, *** corresponding numbers for ZM175 were 613, 1401, and *** most significantly enriched Gene Ontology(GO) terms and KEGG pathways were associated with polyunsaturated fatty acid(PUFA) metabolism in both new and old leaves from *** were associated with photosynthesis and energy metabolism in *** most significantly enriched KEGG pathway in roots of both varieties was “glucosinolate biosynthesis”.In addition, jasmonic acid(JA) concentration in XY60 was higher than in ZM175, although it increased significantly in both varieties following salt *** in relative expression levels of AOS, MYC2, and JAZ revealed by qRT-PCR were concordant with those from *** results suggest that PUFAs may contribute to salt tolerance in common wheat by enhancing the photosynthetic system and JA-related pathways.

关键词： Jasmonic acid Photosynthesis RNA-Seq Salt tolerance Triticum aestivum

Variation in Chromosome Constitution of the Xiaoyan Series Partial Amphiploids and Its Relationship to Stripe Rust and Stem Rust Resistance

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Journal of Genetics and Genomics 2015年第11期42卷 657-660页

作者： Qi Zheng qiaoling luo Zhixia Niu Hongwei Li Bin Li Steven S.Xu Zhensheng Li State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Science Department of Plant Sciences North Dakota State University

The wild relatives of wheat （Triticum aestivum L.） contain tremendous amounts of potentially useful genes and represent a promising source of genetic diversity for wheat improvement （Bommineni and Jauhar, 1997）. Thinopyrum ponticum （Popd.） Barkworth and D. R. Dewey [syn. Agropyron elongatum （Host） P. Beauv., Elytrigia pontica （Podp.） Holub, Lophopy- rum ponticum （Podp.） A. L6ve] （2n = 10x = 70）, has high crossability with various Triticum species. Numerous studies have shown that Th. ponticum carries many potentially valu- able resistance genes against biotic and abiotic stresses （Shannon, 1978; Cox, 1991; Zheng et al., 2014a,b）. Transferring the useful genes from Th. ponticum to common wheat through chromosome engineering had been a successful way to enhance the resistance of wheat to pests and diseases （Sharma et al., 1989; McIntosh, 1991）.

关键词： Th Variation in Chromosome Constitution of the Xiaoyan Series Partial Amphiploids and Its Relationship to Stripe Rust and Stem Rust Resistance FISH GISH IT

Mapping of powdery mildew resistance genes transferred to common wheat from wild emmer wheat revealed three functional Pm60 haplotypes

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The Crop Journal 2024年第2期12卷 540-548页

作者： Wenxin Wei Nannan Liu Shengnan Zhang Jing Zhang Wei Pan Xiaoming Xie Zuhuan Yang Junna Sun Jun Ma Zhaorong Hu Weilong Guo qiaoling luo Jingzhong Xie Fei He Yinghui Li Chaojie Xie Qixin Sun Key Laboratory of Crop Heterosis and Utilization(MOE)/Key Laboratory of Crop Genetic Improvement China Agricultural UniversityBeijing 100193China Institute of Advanced Agricultural Science Peking UniversityWeifang 261200ShandongChina Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Institute of Evolution University of HaifaMt.CarmelHaifa 3498838Israel

Powdery mildew(PM),caused by Blumeria graminis ***(Bgt),is one of the destructive wheat diseases *** emmer wheat(Triticum turgidum ***,WEW),a tetraploid progenitor of common wheat,is a valuable genetic resource for wheat disease resistance breeding *** developed three hexaploid pre-breeding lines with PM resistance genes derived from three WEW *** resistant pre-breeding lines were crossed with susceptible common wheat *** in the F2populations were 3 resistant:1 susceptible,suggesting a single dominant allele in each resistant *** of the resistance gene in each line indicated a single locus on the long arm of chromosome 7A,at the approximate location of previously cloned Pm60 from *** sequencing revealed three different Pm60 haplotypes(Hap 3,Hap 5,and Hap 6).Co-segregating diagnostic markers were developed for identification and selection of each *** resistance function of each haplotype was verified by the virus-induced gene silencing(VIGS).Common wheat lines carrying each of these Pm60 haplotypes were resistant to most Bgt isolates and differences in the response arrays suggested allelic variation in response.

关键词： Alleles Blumeria graminus Marker-assisted selection Molecular marker Triticum dicoccoides

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创意经济时代中国大学与城市互动发展探讨

科技进步与对策 2009年第13期26卷 96-98页

作者：罗巧灵武汉市城市规划设计研究院湖北武汉430014

创意经济在现代城市的崛起,使得大学在城市中的地位越来越重要,大学与城市互动融合发展成为必然趋势。分析了创意经济时代大学在城市中的地位与作用,评析了当前中国高校与城市发展的现状与特点,并提出了大学与城市互动发展的建议。

关键词：创意经济大学城市

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大都市郊区化过程中郊区住区开发模式探讨

城市规划 2007年第3期31卷 25-29页

作者：周婕罗巧灵武汉大学城市建设学院

通过对大都市人口郊区化的动力机制和人口郊区化对住区开发建设影响的分析,借鉴英国的新城建设、美国的边缘城市及新城市主义和巴黎新城开发的经验和教训,探讨我国大都市郊区住区的开发模式,并提出了适于我国大都市郊区的“中心网络型... 详细信息

通过对大都市人口郊区化的动力机制和人口郊区化对住区开发建设影响的分析,借鉴英国的新城建设、美国的边缘城市及新城市主义和巴黎新城开发的经验和教训,探讨我国大都市郊区住区的开发模式,并提出了适于我国大都市郊区的“中心网络型”住区开发模式。

关键词：大都市郊区化人口住区开发模式

融合发展背景下纸、数教材协同编纂实现进路和机制要点

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出版发行研究 2024年第4期 50-57页

作者：罗巧玲朱于国人教数字出版有限公司人民教育出版社

教育出版的未来之路是融合发展之路,从近年发展形势看,纸质教材、数字教材联动研发尚处在探索阶段,暂未形成清晰的发展思路和有序的开发模式。文章以教材出版供给侧结构性改革为出发点,以“协同编纂”这一关键环节为入口,探究融合发展... 详细信息

教育出版的未来之路是融合发展之路,从近年发展形势看,纸质教材、数字教材联动研发尚处在探索阶段,暂未形成清晰的发展思路和有序的开发模式。文章以教材出版供给侧结构性改革为出发点,以“协同编纂”这一关键环节为入口,探究融合发展背景下纸、数教材协同编纂的实现进路和机制要点,为教材出版融合发展作有益探索。

关键词：教材编研协同编纂融合发展纸数联动

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武汉市历史文化遗产动态保护的规划实践探索

城市规划学刊 2013年第5期 94-99页

作者：刘奇志罗巧灵武汉市国土资源和规划局武汉大学城市设计学院武汉市规划研究院

城市历史文化遗产是人类的宝贵财富,探索适宜的保护方式,使其既能延续历史文脉、又能适应城市的发展要求,是当前历史文化遗产保护的重点课题。近年来,武汉市在历史文化保护方面进行了诸多探索,文章从宏观、中观、微观三个层次,介绍了武... 详细信息

城市历史文化遗产是人类的宝贵财富,探索适宜的保护方式,使其既能延续历史文脉、又能适应城市的发展要求,是当前历史文化遗产保护的重点课题。近年来,武汉市在历史文化保护方面进行了诸多探索,文章从宏观、中观、微观三个层次,介绍了武汉市历史文化遗产动态保护的理念与具体做法。

关键词：武汉历史文化遗产动态保护规划

Development of wheat-rye 4R chromosome translocation lines and assignment of its powdery mildew resistance

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Development of wheat-rye 4R chromosome translocation lines a...

第十届全国小麦基因组学及分子育种大会

作者： Pengtao Ma Guohao Han Qi Zheng Shiyu Liu Fangpu Han Jing Wang qiaoling luo Diaoguo An Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of Sciences State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and DevelopmentalBiologyChinese Academy of Sciences

Rye(Secale ceraele L.) is an important gene donor for wheat improvement because of its many valuable traits,especially disease resistance Development of novel wheat-rye translocations with disease resistance can contribute to transferring the resistance into common *** a previous study,a wheat-rye T4 BL-4 RL and T7 AS-4 RS translocation line,WR41-1,was developed by distant hybridization,and it was speculated that its resistance to powdery mildew,caused by Blumeria gra,minis ***(Bgt),was derived from rye based on pedigree analysis To make accurate use of chromosome 4 R in wheat improvement,a set of new 4 R translocations involving different arm translocations,such as 4 RS monosomic,4 RL monosomic,4 RL disomic,4 RS monosomic plus 4 RL monosomic,4 RS monosomic plus 4 RL disomic,and 4 RS disomic plus 4 RL disomic translocations,were developed from crosses with common *** translocations were characterized by genomic in situ hybridization(GISH) and EST-SSR(expressed sequence tag-simple sequence repeat) marker *** confirm the source of powdery mildew resistance,the translocation plants were tested against Bgt isolate E09,the results indicated that all translocations with 4 RL were resistant at all tested growth stages;while those with only 4 RS translocation or no alien translocation were *** indicated that the powdery mildew resistance of WR41-1 was derived from the alien chromosome arm 4 *** effectively use the 4 RL resistance in wheat improvement,two kompetitive allele specific PCR(KASP) markers specific for chromosome arm 4 RL were developed to detect the alien chromosome in the wheat *** new translocation lines with diagnostic markers can efficiently serve as important bridges for wheat improvement.

关键词： Secale ceraele L. powdery mildew translocation line KASP marker

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美国交通政策“绿色”转型、实践及其启示

规划师 2010年第9期26卷 5-10页

作者：罗巧灵 David Martineau 不详美国波特兰州立大学

20世纪以来,美国的联邦交通政策经历了"绿色"转型过程。美国的波特兰市是绿色交通实践的成功案例。通过对美国交通政策转型与波特兰市成功案例的分析与借鉴,指出当前中国构建绿色交通体系需重视4个方面:创建"绿色"城市结构,发展"绿色"... 详细信息

20世纪以来,美国的联邦交通政策经历了"绿色"转型过程。美国的波特兰市是绿色交通实践的成功案例。通过对美国交通政策转型与波特兰市成功案例的分析与借鉴,指出当前中国构建绿色交通体系需重视4个方面:创建"绿色"城市结构,发展"绿色"交通模式,采用"绿色"交通工具,体现"绿色"人文思想。

关键词：交通政策转型绿色交通美国