Mapping of powdery mildew resistance genes transferred to common wheat from wild emmer wheat revealed three functional Pm60 haplotypes

作     者：Wenxin Wei Nannan Liu Shengnan Zhang Jing Zhang Wei Pan Xiaoming Xie Zuhuan Yang Junna Sun Jun Ma Zhaorong Hu Weilong Guo Qiaoling Luo Jingzhong Xie Fei He Yinghui Li Chaojie Xie Qixin Sun Wenxin Wei;Nannan Liu;Shengnan Zhang;Jing Zhang;Wei Pan;Xiaoming Xie;Zuhuan Yang;Junna Sun;Jun Ma;Zhaorong Hu;Weilong Guo;Qiaoling Luo;Jingzhong Xie;Fei He;Yinghui Li;Chaojie Xie;Qixin Sun

作者机构：Key Laboratory of Crop Heterosis and Utilization(MOE)/Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China Institute of Advanced Agricultural SciencePeking UniversityWeifang 261200ShandongChina Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Triticeae Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina Institute of EvolutionUniversity of HaifaMt.CarmelHaifa 3498838Israel 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2024年第12卷第2期

页      面：540-548页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by grants from the National Key Research and Development Program of China(2023YFF1000404,2022YFF10001501) the National Natural Science Foundation of China(32171971) 

主　　题：Alleles Blumeria graminus Marker-assisted selection Molecular marker Triticum dicoccoides 

摘      要：Powdery mildew(PM),caused by Blumeria graminis ***(Bgt),is one of the destructive wheat diseases *** emmer wheat(Triticum turgidum ***,WEW),a tetraploid progenitor of common wheat,is a valuable genetic resource for wheat disease resistance breeding *** developed three hexaploid pre-breeding lines with PM resistance genes derived from three WEW *** resistant pre-breeding lines were crossed with susceptible common wheat *** in the F2populations were 3 resistant:1 susceptible,suggesting a single dominant allele in each resistant *** of the resistance gene in each line indicated a single locus on the long arm of chromosome 7A,at the approximate location of previously cloned Pm60 from *** sequencing revealed three different Pm60 haplotypes(Hap 3,Hap 5,and Hap 6).Co-segregating diagnostic markers were developed for identification and selection of each *** resistance function of each haplotype was verified by the virus-induced gene silencing(VIGS).Common wheat lines carrying each of these Pm60 haplotypes were resistant to most Bgt isolates and differences in the response arrays suggested allelic variation in response.