检索结果-南通市图书馆

M6A RNA methylation modification and tumor immune microenvironment in lung adenocarcinoma

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biophysics Reports 2023年第3期9卷 146-158页

作者： Shujuan li qianzhong li Luqiang Zhang Yechen Qi Hui Bai Laboratory of Theoretical Biophysics School of Physical Science and Technology Inner Mongolia University The State Key Laboratory of Reproductive Regulation and Breeding of Grassland livestock Inner Mongolia University

Lung adenocarcinoma is one of the deadliest tumors. Studies have shown that N6-methyladenosine RNA methylation regulators, as a dynamic chemical modification, affect the occurrence and development of lung adenocarcinoma. To investigate the relationship between mutations and expression levels of m6A regulators in lung adenocarcinoma, we investigated the mutations and expression levels of38 m6A regulators. We found that mutations in m6A regulatory factors did not affect the changes in expression levels, and 19 differentially expressed genes were identified. All tumor samples were classified into two subtypes based on the expression levels of 19 differentially expressed m6A-regulated genes. Survival analysis showed significant differences in survival between the two subtypes. To explore the relationship between immune cell infiltration and survival in both subtypes, we calculated the infiltration of 23 immune cells in both subtypes, and we found that the subtype with high immune cell infiltration had better survival. We found that subtypes with low tumor purity and high stromal and immune scores had better survival. The m6A-related immune genes were identified by taking the intersection of differentially expressed genes and immune genes in the two isoforms and calculating the Pearson correlation coefficients between the intersecting immune genes and the differentially expressed m6A-regulated genes. Finally, a prognostic model associated with m6A and associated with immunity was developed using prognostic genes screened from m6A-associated immune genes. The predictive power of the model was evaluated and our model was able to achieve good prediction.

关键词： Lung adenocarcinoma M6A RNA methylation regulators Consensus clustering Immune cell infiltrates Tumor immune microenvironment

Reference Gene Selection for Quantitative Real-Time PCR Analyses of Acer palmatum under Abiotic Stress

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2020年第2期89卷 385-403页

作者： Lu Zhu Qiuyue Ma Shushun li Jing Wen Kunyuan Yan qianzhong li Institute of Leisure Agriculture Jiangsu Academy of Agricultural SciencesNanjing210014China

Quantitative real-time reverse transcriptase PCR(qRT-PCR)technology has been extensively used to estimate gene expression levels,and the selection of appropriate reference genes for qRT-PCR analysis is critically important for obtaining authentic normalized *** palmatum is an important colorful leaf ornamental tree species,and reference genes suitable for normalization of the qRT-PCR data obtained from this species have not been *** this study,the expression stability of ten candidate reference genes,namely,Actin3,Actin6,Actin9,EF1α,PP2A,SAMDC,TIP41,TUBα,TUBβand UBQ10,in two distinct tissues(leaves and roots)of *** under four different abiotic stress conditions(cold,heat,salt and drought)were investigated and assessed using three statistical methods(GeNorm,NormFinder and BestKeeper).The combinations of reference genes that showed stability in the different stressed samples ***,Actin6,UBQ10 and Actin9 were the most stable reference genes in all the samples,and Actin3 and Actin6 wexre stably expressed in cold-stressed leaves and ***3,Actin6 and UBQ10 were identified as an appropriate combination of reference genes for the analysis of heat-stressed leaves and roots,whereas the combination of Actin9,UBQ10 and Actin6 was deemed the most suitable for the analysis of salt-stressed leaves and ***,Actin6 and UBQ10 exhibited stable expression in drought-stressed leaves and ***,the expression levels of CBF,Cu/Zn-SOD and HsfA1 were estimated to determine the reliability of the reference genes assessed in this *** study revealed stable reference genes in *** that might be used for the normalization of qRT-PCR data obtained under various abiotic stresses.

关键词： Acer palmatum qRT-PCR abiotic stresses referencegene normalization

A prognostic model for acute myeloid leukemia based on ferroptosis-related lncRNA and immune infiltration analysis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biophysics Reports 2024年第6期10卷 377-387页

作者： Shuhan liu Yingli Chen qianzhong li Zhiyu Fan Menglan li Pengyu Du Laboratory of Theoretical Biophysics School of Physical Science and Technology Inner Mongolia University The State Key Laboratory of Reproductive Regulation and Breeding of Grassland livestock Inner Mongolia University

Acute myeloid leukemia(AML) is a rare tumor that invades the blood and bone marrow, it is rapidly progressive, highly aggressive, and difficult to cure. Studies have shown that long non-coding RNA(lncRNA) and ferroptosis play important roles in AML. However, few studies have been done on ferroptosis-related lncRNA for AML. To investigate the role of ferroptosis-related lncRNA in AML prognosis, we screened the differentially expressed genes related to ferroptosis and lncRNA. Ferroptosisrelated lncRNA associated with AML prognosis was obtained by Pearson correlation analysis. By using univariate Cox analysis, least absolute shrinkage and selection operator(LASSO) analysis, and multivariate Cox analysis, the ten prognostic genes were used for constructing the prognostic model. The model was then validated using a Kaplan-Meier analysis and Cox regression analysis. The ROC results have shown that the model could better predict AML survival. We identified some mutated genes that may affect the poor prognosis based on the somatic mutation analysis. The enrichment pathway analysis of prognostic genes revealed that these genes were mainly enriched in some immune pathways and cancer pathways. By immune infiltration analysis, we found that high-risk patients may respond better to immunotherapy.

关键词： Acute myeloid leukemia Ferroptosis Long non-coding RNA Prognosis Immune infiltrates

人类干细胞启动子区CG含量与组蛋白修饰的相关性

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

生物物理学报 2015年第3期31卷 263-271页

作者：苏文霞李前忠内蒙古大学物理科学与技术学院呼和浩特010021

组蛋白修饰与基因的转录表达关系密切,是表观遗传学的主要内容之一。启动子是调控基因表达的重要元件之一。按照Cp G含量的不同,可以将启动子分为高Cp G含量(HCG)和低Cp G含量(LCG)两类。通过比较人类H1细胞系中16种组蛋白修饰在HCG启... 详细信息

组蛋白修饰与基因的转录表达关系密切,是表观遗传学的主要内容之一。启动子是调控基因表达的重要元件之一。按照Cp G含量的不同,可以将启动子分为高Cp G含量(HCG)和低Cp G含量(LCG)两类。通过比较人类H1细胞系中16种组蛋白修饰在HCG启动子及LCG启动子上的分布,发现大部分组蛋白修饰在整个启动子区域都是HCG启动子的修饰水平较高,个别组蛋白修饰在启动子的部分区域上是LCG启动子的修饰水平较高;计算了两类启动子中不同组蛋白修饰之间的相关系数,分别得到了HCG启动子和LCG启动子中特有及共有的组蛋白修饰簇;选取了10个与干细胞自我更新相关的关键转录因子基因,对这10个基因的启动子进行了HCG和LCG分类,并得到了每个启动子上组蛋白修饰的分布特异性。这些结果为研究CG含量对于不同组蛋白修饰的影响,以及组蛋白修饰与CG含量共同调控干细胞自我更新的机制,提供了一些理论依据。

关键词：组蛋白修饰 HCG启动子 LCG启动子干细胞自我更新

A Study of The Regulation of The Allosteric Enzyme Activity

在线全文

学校读者我要写书评

暂无评论

A Study of The Regulation of The Allosteric Enzyme Activity

模拟秋季酸雨对三角枫叶片光合生理特性的影响

CCAST“生物学启发的理论问题”海峡两岸第二届研讨会

作者： qianzhong li lirong Zhang liaofu Luo ' Laboratory of Theoretical Physics and Biology, Inner Mongolia University, Hohhot,010021,China

Based on the properties of allosteric enzymes,a general allosteric model is proposed forallosteric enzyme with n binding *** is proved theoretically that MWC model is a special caseof the *** can explain not only the ...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

西北植物学报 2016年第12期36卷 2484-2490页

作者：唐玲李倩中李淑顺闻婧江苏省农业科学院园艺研究所江苏省高效园艺作物遗传改良重点实验室南京210014

以三角枫幼苗为材料,采用盆栽方法,研究了pH 5.6、pH 4.0、pH 3.0和pH 2.0酸度模拟酸雨胁迫对三角枫叶片光合生理特性的影响,以探讨酸雨胁迫下三角枫的光合生理响应机制。结果显示:(1)随着酸雨浓度的增加和胁迫时间的延长,三角枫叶片相... 详细信息

以三角枫幼苗为材料,采用盆栽方法,研究了pH 5.6、pH 4.0、pH 3.0和pH 2.0酸度模拟酸雨胁迫对三角枫叶片光合生理特性的影响,以探讨酸雨胁迫下三角枫的光合生理响应机制。结果显示:(1)随着酸雨浓度的增加和胁迫时间的延长,三角枫叶片相对叶绿素含量下降幅度逐步增大;叶片丙二醛含量呈逐渐上升的趋势,且各处理均显著高于对照;叶片质膜透性和脯氨酸含量均呈先升高后降低的趋势,且pH 2.0处理的叶片质膜透性在试验20d时迅速增大,升高幅度最大(146.3%)。(2)叶片净光合速率、蒸腾速率、水分利用效率、表观光能利用效率以及表观CO2利用效率在胁迫下也均呈显著下降趋势,且以pH 3.0和pH 2.0处理降幅最大。研究表明,pH 4.0的模拟酸雨对三角枫叶片的光合生理指标无显著影响,而pH≤3.0的强酸雨胁迫使三角枫叶片叶绿素含量显著下降、膜保护系统受损、光合作用效率显著下降;三角枫能适应弱酸雨(pH≥4.0)环境,可作为酸雨地区的园林绿化树种。

关键词：三角枫模拟酸雨光合生理特性

盐胁迫对鸡爪槭幼苗生长及其叶绿素荧光参数的影响

在线全文

学校读者我要写书评

暂无评论

西北植物学报 2015年第10期35卷 2050-2055页

作者：唐玲李倩中荣立苹李淑顺江苏省农业科学院园艺研究所/江苏省高效园艺作物遗传改良重点实验室南京210014

以鸡爪槭幼苗为材料,采用盆栽方法,研究了不同盐浓度[0.042%(对照)、0.2%、0.4%和0.6%]对鸡爪槭幼苗生长的伤害和叶绿素荧光参数的影响。结果显示:当土壤NaCl含量为0.2%、0.4%和0.6%时,鸡爪槭幼苗分别表现为轻度、中度和重度盐害;叶片... 详细信息

以鸡爪槭幼苗为材料,采用盆栽方法,研究了不同盐浓度[0.042%(对照)、0.2%、0.4%和0.6%]对鸡爪槭幼苗生长的伤害和叶绿素荧光参数的影响。结果显示:当土壤NaCl含量为0.2%、0.4%和0.6%时,鸡爪槭幼苗分别表现为轻度、中度和重度盐害;叶片含水量、叶绿素a和b及叶绿素总含量均随盐浓度的增加而显著下降,花色素苷含量则表现为随盐浓度的增大而显著上升,分别比对照高出48.7%、280.3%和382.7%;叶片叶绿素荧光参数PSⅡ潜在活性(Fv/Fo)、潜在量子效率(Fv/Fm)、光化学量子产量(Yield)、光合电子传递速率(ETR)、实际光化学效率(ΦPSⅡ)和光化学猝灭系数(qP)均随着盐浓度的增大呈显著下降趋势,但非光化学猝灭系数(NPQ)在低盐胁迫时则较对照显著提高,0.2%NaCl处理时比对照显著增加33.3%,而高盐胁迫下则显著下降。研究表明,盐胁迫显著抑制了鸡爪槭幼苗叶片叶绿素合成和光合作用进行,而幼苗叶片在低盐胁迫下则可能通过增加PSⅡ反应中心非辐射热能量耗散来保护光合机构不受损害,从而表现出一定的耐盐胁迫能力。

关键词：鸡爪槭盐胁迫叶片含水量色素叶绿素荧光

基于改进状态转移算法的串级平推流反应器动力学参数估计

在线全文

学校读者我要写书评

暂无评论

化工学报 2019年第2期70卷 607-616页

作者：薛永飞王雅琳孙备李钱钟孙家舟中南大学信息科学与工程学院湖南长沙410083 中国科学院自动化研究所北京100190

实际化工过程反应系统通常由若干个相互关联的平推流反应器串级构成,要建立其机理模型,并估计其动力学参数,就必须反复求解大规模非线性微分方程组,计算代价较高。针对智能优化算法在求解昂贵优化问题时计算成本过高,而工业过程模型往... 详细信息

实际化工过程反应系统通常由若干个相互关联的平推流反应器串级构成,要建立其机理模型,并估计其动力学参数,就必须反复求解大规模非线性微分方程组,计算代价较高。针对智能优化算法在求解昂贵优化问题时计算成本过高,而工业过程模型往往只需要找到一组满意解即可的特点,提出了一种基于改进状态转移算法的串级平推流反应器动力学参数估计方法。该算法在保留标准状态转移算法全局寻优能力和快速收敛能力的同时,利用对立算子优化初始种群,并根据误差阈值判定满意解中止条件,可有效节约计算时间。以实际炼油企业的加氢裂化装置为研究对象,采用所提方法对其反应系统的动力学参数进行估计,仿真结果表明了所提方法的有效性。

关键词：串级平推流反应器反应动力学动力学模型参数估值状态转移算法昂贵优化满意解

在线全文

学校读者我要写书评

暂无评论

台州头门港进港航道试挖槽回淤分析

水运工程 2023年第3期 109-113,182页

作者：徐海李前钟黄建华浙江省水利河口研究院(浙江省海洋规划设计研究院) 浙江杭州310020

通过对台州头门港进港航道试挖槽开挖后历次水下地形和断面监测资料进行分析,得出试挖槽回淤特征,并探讨回淤机理。结果表明:13个月的监测期内,试挖槽经历了自然缓慢回淤→骤淤冲刷交替→自然缓慢冲刷的发育过程,最终达到动态平衡稳定,... 详细信息

通过对台州头门港进港航道试挖槽开挖后历次水下地形和断面监测资料进行分析,得出试挖槽回淤特征,并探讨回淤机理。结果表明:13个月的监测期内,试挖槽经历了自然缓慢回淤→骤淤冲刷交替→自然缓慢冲刷的发育过程,最终达到动态平衡稳定,试挖槽平均水深基本满足11.7 m的设计通航水深要求。正常天气条件下,悬沙落淤是试挖槽的主要回淤形式;大风天气条件下,台风的风浪掀沙作用使试挖槽发生骤淤,槽内发育浮泥层,寒潮的作用微弱。

关键词：进港航道试挖槽回淤监测骤淤