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桑辛素对胶质瘤细胞株U87迁移与侵袭的影响

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实用肿瘤学杂志 2024年第3期38卷 168-172页

作者：唐冬高文宏张华平陈谦学长江大学附属荆州医院神经外科荆州434020 武汉大学人民医院神经外科

目的研究桑辛素在体外对人脑胶质母细胞瘤U87细胞迁移和侵袭能力的影响,并探讨其作用机制。方法利用细胞毒性及活性检测试剂盒(Cell counting kit-8,CCK-8)检测桑辛素对U87细胞增殖能力的影响;利用细胞划痕实验及Transwell侵袭实验检测... 详细信息

目的研究桑辛素在体外对人脑胶质母细胞瘤U87细胞迁移和侵袭能力的影响,并探讨其作用机制。方法利用细胞毒性及活性检测试剂盒(Cell counting kit-8,CCK-8)检测桑辛素对U87细胞增殖能力的影响;利用细胞划痕实验及Transwell侵袭实验检测桑辛素对U87细胞迁移和侵袭的影响;Western blot检测桑辛素对U87细胞中波形蛋白(Vimentin)、基质金属蛋白酶2(Matrix metalloproteinas,MMP-2)表达水平的影响。结果CCK-8检测结果显示,与0μg/mL的桑辛素相比,1、2、4、6μg/mL的桑辛素能够显著抑制U87细胞的增殖(P<0.001);划痕实验结果表明桑辛素浓度2、4、6μg/mL组迁移率分别为(20.597±1.225)%、(14.734±1.528)%和(7.811±1.496)%,显著低于0μg/mL组的(40.566±3.284)%(P<0.001);Transwell侵袭实验结果显示,桑辛素浓度2、4、6μg/mL组划痕愈合率分别为(85.000±6.557)、(41.000±6.245)和(13.333±3.215)个,显著低于0μg/mL组(116.667±14.572)(P<0.01);Western blot检测结果显示随着桑辛素浓度的增加,U87细胞中Vimentin的表达水平升高(P<0.001),而MMP-2的表达水平降低(P<0.001)。结论桑辛素能够有效抑制U87细胞的迁移和侵袭能力,其作用在一定的范围内与桑辛素的浓度呈正相关性。

关键词：桑辛素胶质母细胞瘤迁移侵袭

抑制牛磺酸上调基因1通过上调脑源性神经生长因子减轻大鼠脑缺血再灌注损伤

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武汉大学学报（医学版） 2024年第8期45卷 898-902页

作者：李斐江洪祥张惠凯张新元陈谦学武汉大学人民医院神经外科湖北武汉430060

目的:探讨牛磺酸上调基因1(TUG1)在大鼠脑缺血再灌注损伤(CIRI)中的作用及其可能的机制。方法:建立CIRI大鼠模型,分别转染反义寡核苷酸(ASO)-TUG1及其空白对照ASO-NC,将大鼠分为对照组、CIRI组、CIRI+ASO-NC组、CIRI+ASO-TUG1组,采用mNS... 详细信息

目的:探讨牛磺酸上调基因1(TUG1)在大鼠脑缺血再灌注损伤(CIRI)中的作用及其可能的机制。方法:建立CIRI大鼠模型,分别转染反义寡核苷酸(ASO)-TUG1及其空白对照ASO-NC,将大鼠分为对照组、CIRI组、CIRI+ASO-NC组、CIRI+ASO-TUG1组,采用mNSS评估各组大鼠的神经功能,TTC染色观察各组大鼠的脑梗死体积,PCR检测大鼠脑组织中TUG1和脑源性神经生长因子(BDNF) mRNA的表达水平,Western Blot和免疫组化实验检测各组BDNF蛋白的表达。结果:CIRI组大鼠脑组织中TUG1的表达水平显著高于对照组(P<0.05);转染ASO-TUG1可显著降低TUG1的表达水平(P<0.05);CIRI+ASO-TUG1组大鼠的mNSS评分和脑梗死体积显著低于CIRI组和CIRI+ASO-NC组(P<0.05);CIRI+ASO-TUG1组大鼠脑组织中BDNF mRNA和蛋白表达水平均高于CIRI组和CIRI+ASO-NC组(P<0.05)。结论:降低TUG1的表达则可显著增加CIRI大鼠脑组织中BDNF基因和蛋白的表达水平,减少脑梗死体积,改善神经功能。

关键词：牛磺酸上调基因1 脑缺血再灌注损伤脑源性神经生长因子神经功能

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Expression and sub-cellular localization of leucine-rich repeats and immunoglobulin-like domains are related to antioxidant enzymes in human ependymoma and oligodendroglioma

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Neural Regeneration Research 2011年第11期6卷 860-864页

作者： Wei Yi Lin Liu Okechi Humphrey qianxue chen Shulan Huang Department of Neurosurgery Renmin Hospital of Wuhan University Wuhan 430060 Hubei Province China Department of Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

The current study investigated correlations between the expression of leucine-rich repeats and immunoglobulin-like domain 1 （LRIG1） and antioxidant enzymes and related proteins, including manganese superoxide dismutase, glutamate cysteine ligase catalytic or regulatory subunit, thioredoxin and thioredoxin reductase, in both human ependymoma and oligodendroglioma. Results revealed that the cytoplasmic expression of LRIG1 was associated with expression of glutamate cysteine ligase catalytic subunit in the human ependymoma, while the nuclear expression of LRIG1 was associated with expression of thioredoxin reductase. In human oligodendroglioma, the cytoplasmic expression of LRIG1 was associated with expression of the glutamate cysteine ligase catalytic subunit. Both the nuclear and perinuclear expressions of LRIG1 were associated with expression of glutamate cysteine ligase regulatory subunit. These results indicated that several antioxidant enzymes and related proteins contributed to LRIG1 expression, and that these may participate in the antioxidation of the cells.

关键词： leucine-rich repeats and immunoglobulin-like domain 1 sub-cellular localization ependymoma oligodendroglioma antioxidant enzymes

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胶质母细胞瘤诊疗研究进展

肿瘤防治研究 2022年第11期49卷 1103-1106页

作者：陈谦学武汉大学人民医院神经外科武汉430061

胶质母细胞瘤是最常见的原发性颅内恶性肿瘤。尽管在过去的几十年里进行了大量的研究,胶质母细胞瘤的预后仍然很差。近年来随着多组学等研究在胶质母细胞瘤中的应用,加深了人们对胶质母细胞瘤的认识,主要体现在诊断上对其分类进行了重... 详细信息

胶质母细胞瘤是最常见的原发性颅内恶性肿瘤。尽管在过去的几十年里进行了大量的研究,胶质母细胞瘤的预后仍然很差。近年来随着多组学等研究在胶质母细胞瘤中的应用,加深了人们对胶质母细胞瘤的认识,主要体现在诊断上对其分类进行了重新修订。治疗上,在原有的手术和放化疗等标准治疗基础上发展了靶向治疗、免疫治疗和电场治疗等新型治疗方法。本文对胶质母细胞瘤近年来在诊疗中取得的最新进展进行简要述评。

关键词：胶质母细胞瘤分子病理靶向治疗免疫治疗电场治疗

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Brain-targeting,acid-responsive antioxidant nanoparticles for stroke treatment and drug delivery

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Bioactive Materials 2022年第10期7卷 57-65页

作者： Shenqi Zhang Bin Peng Zeming chen Jiang Yu Gang Deng Youmei Bao Chao Ma Fengyi Du Wendy C.Sheu W.Taylor Kimberly J.Marc Simard Daniel Coman qianxue chen Fahmeed Hyder Jiangbing Zhou Kevin N.Sheth Department of Neurosurgery USA Department of Biomedical Engineering USA Department of Radiology and Biomedical Imaging USA Department of Neurology Yale UniversityNew HavenCT06510USA Department of Neurosurgery Renmin Hospital of Wuhan UniversityWuhanHubei430060China Department of Neurology Division of Neurocritical CareMassachusetts General HospitalBostonMAUSA Department of Neurosurgery University of Maryland School of MedicineBaltimoreMD21201USA

Stroke is the leading cause of death and ***,there is no effective pharmacological treatment for this disease,which can be partially attributed to the inability to efficiently deliver therapeutics to the *** we report the development of natural compound-derived nanoparticles(NPs),which function both as a potent therapeutic agent for stroke treatment and as an efficient carrier for drug delivery to the ischemic ***,we screened a collection of natural nanomaterials and identified betulinic acid(BA)as one of the most potent antioxidants for stroke ***,we engineered BA NPs for preferential drug release in acidic ischemic tissue through chemically converting BA to betulinic amine(BAM)and for targeted drug delivery through surface conjugation of AMD3100,a CXCR4 *** resulting AMD3100-conjugated BAM NPs,or A-BAM NPs,were then assessed as a therapeutic agent for stroke treatment and as a carrier for delivery of NA1,a neuroprotective *** show that intravenous administration of A-BAM NPs effectively improved recovery from stroke and its efficacy was further enhanced when NA1 was *** to their multifunctionality and significant efficacy,we anticipate that A-BAM NPs have the potential to be translated both as a therapeutic agent and as a drug carrier to improve the treatment of stroke.

关键词： Drug delivery Acid-triggered release Antioxidant nanoparticles NA1 Ischemic stroke

Red light-driven generation of reactive oxygen species for the targeted oxidation of glioma cells and thiols over covalent organic framework

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Chinese Chemical Letters 2023年第12期34卷 509-513页

作者： Heng Wei Xia Li Fengwei Huang Shujuan Wu Huimin Ding qianxue chen Mingchang Li Xianjun Lang Renmin Hospital of Wuhan University College of Chemistry and Molecular SciencesWuhan UniversityWuhan 430072China

Reactive oxygen species(RoS)are essential for biological processes like cell signaling and chemical processes like organic ***,the sufficient generation of RoS plays a significant role in targeted tumor treatments or oxidation of ***,a hydrazone-linked porphyrin covalent organic framework(Por-DETH-COF)is developed for red light-induced generation of ROS like singlet oxygen(^(1)O_(2))or superoxide(O_(2)·^(-))to undertake different but targeted ***,^(1)O_(2)is adopted in photodynamic therapy(PDT)for the oxidation of glioma *** PDT efficiency of Por-DETH-COF on the apoptosis of glioma cells is explored through flow cytometry and western blot *** apoptosis rate of glioma cells significantly increases over Por-DETH-COF under 660nm red light illumination,suggestive of the potency of^(1)O_(2).Second,O_(2)^(·-)is employed for the targeted oxidation of thiols.A series of thiols could be efficiently oxidized to corresponding disulfides over Por-DETH-COF under 660 nm red light illumination,indicative of the significance of O_(2)^(·-).This work highlights the potential of covalent organic frameworks in generating Ros for precise medical applications of complex chemical environments.

关键词： Reactive oxygen species Covalent organic framework Oxidation Photodynamic therapy Glioma cells

基于机器学习早期预测蛛网膜下腔出血后迟发性脑缺血和不良预后的研究进展

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中国脑血管病杂志 2022年第7期19卷 504-508页

作者：胡平叶立果邓钢陈谦学武汉大学人民医院神经外科 430060

迟发性脑缺血是动脉瘤性蛛网膜下腔出血患者中最常见的并发症,也是大多数幸存者出现运动功能缺陷、认知功能障碍等不良预后和生活质量下降的最重要的可预防原因。早期准确预测蛛网膜下腔出血后迟发性脑缺血和不良预后对患者及时干预非... 详细信息

迟发性脑缺血是动脉瘤性蛛网膜下腔出血患者中最常见的并发症,也是大多数幸存者出现运动功能缺陷、认知功能障碍等不良预后和生活质量下降的最重要的可预防原因。早期准确预测蛛网膜下腔出血后迟发性脑缺血和不良预后对患者及时干预非常关键,机器学习作为人工智能领域的一个分支,是预测上述结局事件的最重要方法。作者就近年报道过早期预测蛛网膜下腔出血后迟发性脑缺血和不良预后的机器学习算法模型以及评价指标的文献进行综述,以期帮助临床医师能够通过机器学习算法模型早期预测迟发性脑缺血和不良预后的发生,为临床决策提供依据。

关键词：迟发性脑缺血蛛网膜下腔出血预后人工智能机器学习综述

The important role of Computed Tomography Angiography to predict operation effect and prognosis of severe traumatic brain injury

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The important role of Computed Tomography Angiography to pre...

PDCA式案例教学法在神经外科住院医师规范化培训中的应用

第十五届中国医师协会神经外科医师年会

作者： Junhui chen Mingchang Li Yuhai Wang qianxue chen Department of Neurosurgery Renmin Hospital of Wuhan University Department of Neurosurgery 904~(th) Hospital of Joint Logistic Support Force of PLAWuxi Clinical College of Anhui Medical University Department of Orthopedic 904~(th) Hospital of Joint Logistic Support Force of PLAWuxi Clinical College of Anhui Medical University

Background It was difficult to predict the operative effect and outcome for severe traumatic brain injury(TBI) before *** present of active contrast extravasation at computed tomography angiography(CTA) can predicts in-hospital mortality in patients with nontraumatic intracerebral *** study aims to approve an evaluated method of CTA to predict the effect of operation and outcome in severe *** From 8760 patients with a TBI treated between January 2010 and January 2016,we retrospectively reviewed 358 severe TBI patients who presented to our emergency department and underwent CTA at admission and a follow-up CTA *** experienced readers,blinded to clinical data,reviewed the CTAs to assess for the presence of cerebrovascular changes included cerebrovascular shift(CS),cerebral vasospasm(CVS),large artery occlusion(LAO) and deep venous system occlusion(DVSO).Medical records were reviewed for baseline clinical characteristics and the relationship between cerebrovascular changes and operation effect and 6 months *** Cerebrovascular changes was identified in 197(55.0%) of 358 severe *** hundred seventeen(32.7%) had CS,and just 9(7.7%) of them died,63(53.8%) patients had good ***-four(21.0%) patients had CVS,31(41.9%) had a good outcome,25(33.8%) had severe disability and 18(24.3%) *** were twenty-five(7.0%) patients had LAO and thirty(8.4%) patients had DVSO,all these 55(100%) patients were died regardless whether they had *** Cerebrovascular injuries and changes are frequent after severe TBI,and correlates with prognosis *** is an important tool in evaluating the severity,predicting operation effect and prognosis,and guiding therapy of severe ***,this is a single-hospital retrospectively ***,well-designed,multicentre,randomized controlled trials needed to evaluate the value of the CTA for severe TBI.

关键词： severe TBI CTA Cerebrovascular CVS

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中国继续医学教育 2023年第10期15卷 86-90页

作者：李斐陈谦学武汉大学人民医院神经外科湖北武汉430060

目的探讨基于计划(plan)→执行(do)→检查(check)→处理(action)(PDCA)模式的案例教学法(case-based learning,CBL)在神经外科住院医师规范化培训的临床教学效果。方法选取2020年1月—2022年2月216名在武汉大学人民医院神经外科接受住... 详细信息

目的探讨基于计划(plan)→执行(do)→检查(check)→处理(action)(PDCA)模式的案例教学法(case-based learning,CBL)在神经外科住院医师规范化培训的临床教学效果。方法选取2020年1月—2022年2月216名在武汉大学人民医院神经外科接受住院医师规范化培训的住院医师,随机平均分为对照组和观察组,对照组采用传统教学方法,观察组采用PDCA式CBL教学模式,采用问卷调查及综合考评的方法评估两种不同方法的教学效果。结果理论考试成绩显示观察组学生的成绩(91.83±5.65)分高于对照组(80.02±6.40)分,临床操作考试成绩显示观察组学生的临床实践能力测试成绩(86.00±6.77)分高于对照组(75.89±5.56)分,差异均有统计学意义(P<0.001);问卷调查结果显示观察组学生的教学认可度和满意度均高于对照组,差异有统计学意义(P<0.01)。结论PDCA式CBL教学模式有利于培养学生的学习兴趣,提高住院医师的临床实践能力,提高临床课程的教学质量,在神经外科住院医师规范化培训的教学过程中具备一定的优势。

关键词： PDCA循环案例教学法神经外科住院医师规范化培训理论临床实践