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Sensitive determination of inosine RNA modification in single cell by chemical derivatization coupled with mass spectrometry analysis

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Chinese Chemical Letters 2023年第10期34卷 98-101页

作者： Wan-bing Tao Neng-Bin Xie qing-Yun Cheng Yu-qi Feng Bi-Feng yuan College of Chemistry and Molecular Sciences Department of Radiation and Medical OncologyZhongnan Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China School of Public Health Wuhan UniversityWuhan 430071China Research Center of Public Health Renmin Hospital of Wuhan UniversityWuhan 430060China Cancer Precision Diagnosis and Treatment and Translational Medicine Hubei Engineering Research Center Zhongnan Hospital of Wuhan UniversityWuhan Research Center for Infectious Diseases and CancerChinese Academy of Medical SciencesWuhan 430071China

Inosine is a vital RNA modification across three kingdoms of *** has been demonstrated that inosine plays important roles in modulation of the fate of *** the current study,we developed a highly sensitive method to determine inosine in a single cell by N-cyclohexyl-N’-β-(4-methylmorpholinium)ethylcarbodiimide p-toluenesulfonate(CMCT)derivatization in combination with mass spectrometry *** results showed that the detection sensitivity of inosine was increased by 556-fold after CMCT derivatization,with the limit of detection(LOD)being 4.5 *** the established method,we could detect inosine from 13.0 pg of total RNA of HEK293T ***,inosine in RNA from a single cell could also be clearly detected due to the improved detection ***,we found the level of inosine in RNA of sleep-deprived mice was significantly increased compared to the control mice,indicating that inosine is associated with sleep behavior and might be a potential indicator of sleep *** together,the chemical derivatization coupled with mass spectrometry analysis offers a valuable tool in determination of endogenous RNA modifications in a single cell,which should benefit the functional study of RNA modification in rare clinical samples.

关键词： Inosine RNA modification CMCT Derivatization Mass spectrometry Single cell

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Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： Xihang Chen Zhifeng Chen Menghua Li Weiwei Guo Shuolong yuan Liangwei Xu Chang Lin Xi Shi Wei Chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

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Comparisons of Yield and Growth Behaviors of Hybrid Rice Under Different Nitrogen Management Methods in Tropical and Subtropical Environments

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Journal of Integrative Agriculture 2013年第4期12卷 621-629页

作者： Ibrahim Md PENG Shao-bing TANG qi-yuan HUANG Min JIANG Peng ZOU Ying-bin College of Agronomy Hunan Agricultural University Crop Physiology and Production Center (CPPC) Huazhong Agricultural University

To compare the grain yield and growth behaviors of hybrid rice, field experiments were conducted in a subtropical environment in Changsha, Hunan Province, China, and in two tropical environments in Gazipur and Habiganj in Bangladesh during 2009 to 2011. Three hybrid rice cultivars were grown under three nitrogen （N） management treatments in each experiment. The results showed that grain yield was significantly affected by locations, N treatments and their interaction but not by cultivars. Changsha produced 8-58% higher grain yields than Bangladesh locations. Sink size （spikelet number per unit land area） was responsible for these yield differences. Larger panicle size （spikelet number per panicle） contributed to greater sink size in Changsha. Aboveground total biomass was greater in Changsha than in Bangladesh locations, whereas harvest index was higher in Bangladesh locations than in Changsha. Crop growth rate （CGR） was greater at Changsha than Bangladesh locations during vegetative phase, while the difference was relatively small and not consistent during the later growth phases. Higher leaf area index and leaf area duration were partly responsible for the greater CGR in Changsha. Real-time N management （RTNM） produced lower grain yields than fixed-time N management in more than half of the experiments. Our study suggested that further improvement in rice yield in the tropical environments similar to those of Bangladesh will depend mainly on the ability to increase panicle size as well as CGR during vegetative phase, and the chlorophyll meter threshold value used in RTNM needs to be modified according to environmental conditions and cultivar characteristics to achieve a desirable grain yield.

关键词： crop growth rate biomass production hybrid rice leaf area duration leaf area index real-time nitrogenmanagement

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Digital Watermarking Secure Scheme for Remote Sensing Image Protection

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China Communications 2020年第4期17卷 88-98页

作者： Guanghui yuan qi Hao Space star technology co. LTDBeijing 100086China

As a means of copyright protection for multimedia data, digital watermarking technology has attracted more and more attention in various research fields. Researchers have begun to explore the feasibility of applying it to remote sensing data recently. Because of the particularity of remote sensing image, higher requirements are put forward for its security and management, especially for the copyright protection, illegal use and authenticity identification of remote sensing image data. Therefore, this paper proposes to use image watermarking technology to achieve comprehensive security protection of remote sensing image data, while the use of cryptography technology increases the applicability and security of watermarking technology. The experimental results show that the scheme of remote sensing image digital watermarking technology has good performance in the imperceptibility and robustness of watermarking.

关键词： data security watermark remote sensing image protection

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Universal Concentration Scaling on Rheometric Properties of Polydisperse and High Molecular Weight Polyacrylamide Aqueous Solutions

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Chinese Journal of Polymer Science 2022年第11期40卷 1432-1440页

作者： yuan-qi Fan Alfredo Lanzaro Xue-Feng yuan Institute for Systems Rheology Guangzhou UniversityHigher Education Mega-CenterGuangzhou510006China

A great progress has been made over the last decades in studying concentration scaling on rheometric properties of monodisperse polymer ***,the effects of polydisperse polymer solutions on such a concentration scaling remain *** this work,rheometric properties of industrially relevant polydisperse and high molecular weight polyacrylamide(PAAm)aqueous solution have been *** results show a concentration scaling of the characteristic relaxation time,the plateau modulus and the zero-shear viscosity across a concentration range from 10c^(*)to 250c^(*).The time-concentration superposition principle is validated and extended in the data analysis of the terminal dynamic *** concentration scaling exponent of their shifting factors is significantly smaller than the results of monodisperse polymer solutions in good andθsolvents reported in the *** steady shear viscosity and shear stress of 18M PAAm aqueous solutions with relatively lower concentration(≤35c^(*))could also be superimposed into a master curve with the shear-thinning exponent of 0.73±0.03 and0.27±0.03,respectively,over a wide range of shear rates in about six orders of ***,for 18M PAAm aqueous solutions with higher concentration(≥48c^(*))in an intermediate shear thinning regime,the scaling exponent shows a pronounced concentration *** shear thinning exponent of steady shear viscosity varies from 0.73 to 0.57 as concentration is increased,and then increases from 0.57 to 0.90from sufficiently high shear *** increasing shear rate,the shear-thinning exponent of 18M PAAm aqueous solutions at all concentrations converges to the lower bounded value observed in the relatively less concentrated(≤35c^(*))18M PAAm aqueous solutions,i.e.,0.73±0.02 for shear viscosity and 0.27±0.02 for steady shear stress,*** reveals that the concentration effects of polydisperse polymer solutions could be greatly reduced by the dynamic"molecular indi

关键词： Polymer solution Polydispersity Rheometric properties Time-concentration superposition principle

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The epidermal immune microenvironment plays a dominant role in psoriasis development,as revealed by mass cytometry

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Cellular & Molecular Immunology 2022年第12期19卷 1400-1413页

作者： yuan Zhou Fan Xu Xue-Yan Chen bing-Xi Yan Zhao-yuan Wang Si-qi Chen Min Zheng Xiao-Yong Man Department of Dermatology Second Affiliated HospitalZhejiang University School of MedicineHangzhouChina

Psoriasis is a common chronic inflammatory skin *** diversity and heterogeneity of immune cells in human skin have been studied in recent years,but the spatial distribution of immune cells at the single-cell level in the human psoriatic epidermis and dermis remains *** this study,we mapped psoriatic skin immune cells from paired lesional,perilesional,and nonlesional skin samples using mass *** dendritic cells(DCs)were found in the psoriatic epidermis and *** dermal CD1c^(+)CD11b^(+)cDC2s migrated to the epidermis in the perilesional skin during the preinitiation ***1c^(+)CD11b^(+)cDC2s rapidly replaced EpCAM^(+)CD11c^(low) LC cells and initiated ***,CD207^(+)CD11c^(hi) LC and CD5^(+)T cells accumulated in the psoriatic epidermis and orchestrated epidermal inflammation in *** immune cell pool in the psoriatic dermis primarily included APCs and T ***,unlike that in the dermis,the epidermal immune environment was more significant and coincided with the inflammation occurring during psoriasis.

关键词： Psoriasis Cutaneous immune Mass cytometry Dendritic cell

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Dauricine can inhibit the activity of proliferation of urinary tract tumor cells

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Asian Pacific Journal of Tropical Medicine 2012年第12期5卷 973-976页

作者： Jun Wang yuan Li Xiong-bing Zu Min-Feng Chen Lin qi Department of Urology Xiangya HospitalCentral South University

Objective:To explore the anti-tumor effects of asiatic moonseed rhizome extraction-dauricine on bladder cancer EJ cell strain,prostate cancer PC-3Mcell strain and primary cell culture ***:The main effective component-phenolic alkaloids of Menispermum dauricum was extracted and separated from asiatic moonseed rhizome by chemical *** method was used to detect dauricine anti-tumor ***:Dauricine had an obvious proliferation inhibition effect on the main tumor cells in urinary *** minimum drug sensitivity concentration was between ***μg/mL,and the inhibition ratio increased with the increase of ***:Dauricine,the main effective component extracted from asiatic moonseed rhizome,had a good inhibition effect on tumor cells in urinary *** the same time,Dauricine has certain inhibition effects on the primary cultured tumor cell.

关键词： PAMD Dauricine Urinary system EJ cell strain PC-3Mcell strain

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Nanowire-mediated miRNA delivery

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Science China(Life Sciences) 2024年第11期67卷 2532-2534页

作者： yuan Wang bing Chen Laboratory for Biomaterials and Drug Delivery Department of AnesthesiologyDivision of Critical Care MedicineBoston Children’s HospitalHarvard Medical SchoolBoston 02115USA College of Electronic and Optical Engineering and College of Flexible Electronics(Future Technology) Nanjing University of Posts and TelecommunicationsNanjing 210023China

In the ever-evolving nanomedicine and biotechnology,the pursuit of advanced drug delivery systems that induce precise positioning and targeting in the complex biological landscape for personalized and efficient therapies has become a paramount endeavor(Vargason et al.,2021).Central to this trend is the delivery of functional microRNAs(miRNAs),which play crucial roles in regulating gene expression(Li et al.,2021).Specifically,by binding to target messenger RNAs(mRNAs),miRNAs can control protein synthesis or lead to mRNA degradation through various cellular processes such as differentiation,proliferation,apoptosis,and immune *** processes are essential for regenerative medicine,immune modulation,and disease treatment(e.g.,cancers,chronic infections and autoimmune disorders)where gene expression is dysregulated(Kara et al.,2022).

关键词： precise landscape

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Physical Layer Security Scheme With AoI-Awareness for Industrial IoT Based on Covert Communications

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IEEE/CAA Journal of Automatica Sinica 2025年第1期 276-278页

作者： Yaping Li Zhi-Xin Liu Jia-Wei Su Ya-Zhou yuan the Key Laboratory of Industrial Computer Control Engineering of Hebei Province Yanshan University

Dear Editor,Industrial Internet of things(IIoT) is a typical application of cyberphysical system(CPS). In the IIoT, wireless communication is an inevitable trend to replace the deployment-limited wired transmission for cases with large-scale and mobile devices. However, wireless communication gives rise to critical issues related to physical security, such as malicious detections and attacks [1].

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Microstructure and giant baro-caloric effect induced by low pressure in Heusler Co_(51)Fe_(1)V_(33)Ga_(15) alloy undergoing martensitic transformation

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Journal of Materials Science & Technology 2021年第14期73卷 76-82页

作者： Kai Liu Hai Zeng Ji qi Xiaohua Luo Xuanwei Zhao Xianming Zheng yuan yuan Changcai Chen Shengcan Ma Ren Xie bing Li Zhenchen Zhong Jiangxi Key Laboratory for Rare Earth Magnetic Materials and Devices/Institute for Rare Earth Magnetic Materials and Devices(IREMMD) College of Rare EarthsJiangxi University of Science and TechnologyGanzhou341000China School of Materials Science and Engineering Faculty of Materials Metallurgy and ChemistryJiangxi University of Science and TechnologyGanzhou341000China Henan Key Laboratory of Photovoltaic Materials and School of Physics&Electronics Henan UniversityKaifeng475004China Shenyang National Laboratory(SYNL)for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang110016China School of Materials Science and Engineering University of Science and Technology of ChinaHefeiChina Guizhou Province Key Laboratory for Photoelectrics Technology and Application College of PhysicsGuizhou UniversityGuiyang550025China

Solid-state refrigeration based on the magneto-or mechano-caloric effect,including elasto-and barocaloric in ferroic phase transition materials is promising to replace the current vapor compression refrigeration in consideration of environmental-friendliness and ***,both high driven field and small thermal changes in all of these caloric materials hinder the development of solid-state *** we report a giant baro-caloric effect near room temperature induced by a low hydrostatic pressure in Co-based Co_(51)Fe_(1) V_(33)Ga_(15) Heusler *** maximum adiabatic temperature change under the applied pressure change ofΔp=0.1-100 MPa can be as high asΔ_(Tad)^(Max)=7.7 K(Δ_(Tad)^(Max)/Δpreaches up to~7.7 K kbar-1),surpassing theΔ_(Tad)^(Max)/Δpvalue reported hitherto in baro-caloric *** addition,the microstructure is also studied by using the electron *** with the austenite and martensite,the submicron V-rich particles are precipitated in this alloy,which are believed to account for enhancing mechanical properties.

关键词： Solid-state refrigeration Barocaloric effect Adiabatic temperature change Isostatic pressure Martensitic transformation Co_(51)Fe_(1)V_(33)Ga_(15)alloy

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