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Sequence Analysis and Structural Prediction of the Severe Acute respiratory Syndrome Coronavirus nsp5

中华医学会呼吸病学年会——2011（第十二次全国呼吸病学学术会议）

作者： qi guo) 1 guangdong medical college 2 guangzhou institute of respiratory diseases(State Key Laboratory of respiratory diseases)

Background The CURB-65 score is a simple well validated tool for the assessment of severity in community-acquired pneumonia(CAP).Whether each of the criteria is of equal weight is not clear. The purpose of this study was to determine the weight of CURB-65. Methods 1230 adult patients admitted to our hospital from 2005 to 2009 for CAP were reviewed retrospectively.

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 473-479页

作者： Jia-Hai LU Ding-Mei ZHANG guo-Ling WANG Zhong-Min guo Juan LI Bing-Yan TAN Li-Ping OU-YANG Wen-Hua LING Xin-Bing YU Nan-Shan ZHONG~1 The Public Health School of Sun Yat-Sen University,guangzhou 510080,China 1 guangzhou institute of respiratory diseases,guangzhou medical college,guangzhou 510120,China The Public Health School of Sun Yat-Sen University Guangzhou 510080 China. jiahailu@***

The non-structural proteins (nsp or replicase proteins) of coronaviruses are relativelyconserved and can be effective targets for *** studies have been conducted into the function of thesevere acute respiratory syndrome coronavirus (SARS-CoV) *** this study,bioinformatics methodswere employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD *** and sequential comparison was performed to analyze the mutation trend of the polymerasensp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool(BLASTN) and a protein-protein basic local alignment search tool (BLASTP).The results indicated that thensp5 gene was steady during the epidemic process and the protein was homologous with other coronavirusnsp5 *** protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodiumdodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).This study provided the foundation forfurther exploration of the protein's biological function,and contributed to the search for anti-SARS-CoVdrugs.

关键词： severe acute respiratory syndrome (SARS) severe acute respiratory syndrome coronavirus (SARS-CoV) non-structural protein (nsp) replicase secondary structure 3-D structure

Purification and partial characterizations of coagulant protein Fla from Daboia russelli siamensis (Myanmar) venom

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Acta Pharmacologica Sinica 2007年第10期28卷 1580-1584页

作者： Huan-huan SUN~(1,3),qi CHEN~(2,3),Xi LIN~1,Jia-shu CHEN~(1,4),Peng-xin qiU~1,Guang-mei YAN~~1 Department of Pharmacology,Zhongshan medical college,Sun Yat-Sen University guangzhou 510080,China ~2 guangdong institute forDrug Control,guangzhou 510000,China Department of Pharmacology Zhongshan Medical College Sun Yat-Sen University Guangzhou China guangdong institute for Drug Control Guangzhou China

Aim:To purify and characterize the coagulant protein FIa from Daboia russellisiamensis (Myanmar) ***:FIa was purified from Daboia russellisiamensis (Myanmar) venom by ion-exchange chromatography on CM-SephadexC-50,and gel filtration on Sephadex G-75 and a Superdex 75 *** hemo-static activity of FIa was determined by the method of Williams and *** chromogenic substrates were used respectively to determine the activa-tion of factor X and *** fibrinogen-clotting activity of FIa wasdetermined by the method of Gao et *** saline was used as a negativecontrol while factor Xa and thrombin were used as positive controls,***:FIa,a coagulant protein,was achieved by ion-exchange chromatogra-phy and gel filtration with a molecular weight of 34 479 and an isoelectric point *** was shown to have strong hemostatic *** hemostatic activity of0.5 mg FIa was equal to that of 1.5625 u *** primarily activated factor X,however,had no influence on prothrombin,nor did it cleave or clot ***:FIa is a factor X-activating enzyme,which could activate factor X tofactor Xa,but has no effect on prothrombin and fibrinogen.

关键词： snake venoms Daboia russelli siamensis factor X blood coagulation

Preparation and development of equine hyperimmune globulin F（ab'）2 against severe acute respiratory syndrome coronavirus

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Acta Pharmacologica Sinica 2005年第12期26卷 1479-1484页

作者： Jia-hai LU~(2,8,9) Zhong-min guo~(2,9) Wen-yu HAN~(3,9) guo-ling WANG~(2,9) Ding-mei ZHANG~2 Yi-fei WANG~4 Sheng-yun SUN~4 qin-he YANG~4 Huan-ying ZHENG~5 Bing L WONG~6 Nan-shan ZHONG~7 2 Sun Yat-sen University,guangzhou 510080,China 3 The Agriculture School of Jilin University,Changchun 130062,China 4 Ji-nan University,guangzhou 510632,China 5 Center for Disease Control and Prevention of guangdong Province,guangzhou 510300,China 5 Advantek Biologics,Hong Kong,China 7 guangzhou institute of respiratory diseases,guangzhou medical college,guangzhou 510120,China Sun Yat-sen University Guangzhou 510080 China. jiahailu@***

Aim:The resurgence of severe acute respiratory syndrome(SARS)is still a threatbecause the causative agent remaining in animal reservoirs is not fully understood,and sporadic cases continue to be *** high titers of anti-SARShyperimmune globulin to provide an alternative pathway for emergent future pre-vention and treatment of ***:SARS coronavims(CoV)F69(AY313906)and Z2-Y3(AY394989)were isolated and identified from 2 different Cantoneseonset SARS *** was prepared from SARS-CoV F69 *** horses were immunized 4 times and serum was collected periodically tomeasure the profile of specific IgG and neutralizing antibodies using indirect en-zyme-linked immunosorbent assay and a microneutralization *** were col-lected in large amounts at the peak,where IgG was precipitated using ammoniumsulphate and subsequently digested with *** product was then purifiedusing anion-exchange chromatography to obtain F(ab′)2 fragments,Results:Thespecific IgG and neutralizing antibody titers peaked at approximately week 7 afterthe first immunization,with a maximum value of 1:*** sera collected at thepeak were then *** of approximately 15 g F(ab′)2 was obtained from1 litre antiserum and the purity was above 90% with the titer of 1:5120,which couldneutralize the other strain(SARS-CoV Z2-Y3)as ***:This researchprovides a viable strategy for the prevention and treatment of SARS coronavirusinfection with equine hyperimmune globulin,with the purpose of combating anyresurgence of SARS.

关键词： severe acute respiratory syndrome coronavirus neutralizing antibodies hyperimmune globulin cross protection F(ab’)2 fragments

Association between MTHFR c.677C>T variant and erectile dysfunction among males attending fertility clinic

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Asian Journal of Andrology 2024年第1期26卷 41-45页

作者： Shun Bai Ming-Zhen Li Yang-Yang Wan Xue-Chun Hu Yi-Xun Liu Xian-Hong Tong Tong-Hang guo Lu Zong Ran Liu Yuan-qi Zhao Ping Xiang Bo Xu Xiao-Hua Jiang Department of Gynecology and Obstetrics Reproductive and Genetic HospitalThe First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China NHC Key Laboratory of Male Reproduction and Genetics Guangdong Provincial Reproductive Science Institute(Guangdong Provincial Fertility Hospital)Guangzhou 510600China Department of Urology The First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Wannan medical college Wuhu 241002China

Genetic risk factors have been shown to contribute to the development of sexual ***,the role of methylenetetrahydrofolate reductase(MTHFR)gene variants in the risk of erectile dysfunction(ED)remains *** this study,we recruited 1254 participants who underwent ED assessed by the International Index of Erectile *** MTHFR c.677C>T variant was also measured by fluorescence polymerase chain reaction(PCR).No significant difference in the genotypic frequency of the MTHFR C677T polymorphism(CC,CT,and TT)was observed between men from the ED and non-ED *** addition,on binary logistic regression analysis,both crude and adjusted models showed that the risk of ED was not significantly associated with the C677T ***,a significantly higher frequency of the 677TT polymorphism was found in severe and moderate ED(P=O.02).The positive correlation between the MTHFR 677TT polymorphism and severe ED was confirmed by logistic regression analysis,even after adjusting for potential confounders(odds ratio[OR]=2.46,95%confidence interval[CI]:1.15-5.50,P=0.02).These findings suggest a positive correlation between the MTHFR 677TT polymorphism and the risk of severe *** of MTHFR gene polymorphisms may provide complementary information for ED patients during routineclinicaldiagnosis.

关键词： erectile dysfunction gene variant genetic risk factors methylenetetrahydrofolate reductase sexual function

Method for the multi-view estimation of fish mass using a two-stage neural network with edge-sensitive module

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International Journal of Agricultural and Biological Engineering 2024年第3期17卷 222-229页

作者： Zeyu Jiao Yingjie Cai qi Zhang Zhenyu Zhong guangdong Key Laboratory of Modern Control Technology Institute of Intelligent ManufacturingGuangdong Academy of SciencesGuangzhou 510070China Department of Electronic Engineering The Chinese University of Hong KongHong Kong 999077China college of Economics and Management Northwest A&F UniversityYangling 712100ShaanxiChina

The estimation of fish mass is one of the most basic and important tasks in *** the mass of fish at different growth stages is of great significance for feeding,monitoring the health status of fish,and making breeding plans to increase *** existing estimation methods for fish mass often stay in the 2D plane,and it is difficult to obtain the 3D information on fish,which will lead to the *** solve this problem,a multi-view method was proposed to obtain the 3D information of fish and predict the mass of fish through a two-stage neural network with an edge-sensitive *** the first stage,the side-and downward-view images of the fish and some 3D information,such as side area,top area,length,deflection angle,and pitch angle,were captured to estimate the size of the fish through two vertically placed *** the area of the fish at different views was estimated accurately through the pre-trained image segmentation neural network with an edgesensitive *** the second stage,a fully connected neural network was constructed to regress the fish mass based on the 3D information obtained in the previous *** experimental results indicate that the proposed method can accurately estimate the fish mass and outperform the existing estimation methods.

关键词： fish mass multi-view estimation two-stage neural network edge-sensitive module image segmentation

Cadmium and Zinc Accumulation in Maize Grain as Affected by Cultivars and Chemical Fixation Amendments

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Pedosphere 2011年第5期21卷 650-656页

作者： guo Xiao-Fang WEI Ze-Bin WU qi-Tang qiU Jin-Rong ZHOU Jian-Li Key Laboratory of Ecological Agriculture of Ministry of Agriculture of China College of Natural Resources and Environ- ment South China Agricultural University Guangzhou 510642 (China) South China institute of Environmental Sciences Ministry of Environmental Protection Guangzhou 510655 (China) college of Agriculture Yangtze University Jingzhou 434025(China)

A field experiment was conducted to investigate the effects of soil amendments(lime,nano-Si foliar solution and used diatomite) on the growth and metal uptake of three maize(Zea mays L.) cultivars grown in a Cd and Zn-contaminated acidic *** addition of lime significantly increased the maize grain yields and decreased the concentrations of Zn and Cd in the grains and shoots of maize when compared with the *** the three maize cultivars,Yunshi-5 accumulated the lowest amounts of Cd and Zn in the *** concentrations of Zn and Cd in the grain of Yunshi-5 conformed to the Chinese feed *** data revealed that a combination of low metal-accumulating maize and chemical fixation could effectively provide a barrier to prevent metals from entering the human food chain.

关键词： grain yield heavy metal low metal-accumulating cultivar Zea mays L.

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Integrated multi-omics analysis reveals liver metabolic reprogramming by fish iridovirus and antiviral function of alpha-linolenic acid

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Zoological Research 2024年第3期45卷 520-534页

作者： Lin Liu Ya Zhang Meng-Di Yuan Dong-Miao Xiao Wei-Hua Xu qi Zheng qi-Wei qin You-Hua Huang Xiao-Hong Huang college of Marine Sciences South China Agricultural UniversityGuangdong Laboratory for Lingnan Modern AgricultureGuangzhouGuangdong 510642China Nansha-South China Agricultural University Fishery Research institute GuangzhouGuangdong 511464China Southern Marine Science and Engineering guangdong Laboratory ZhuhaiGuangdong 519082China

Iridovirus poses a substantial threat to global aquaculture due to its high mortality rate;however,the molecular mechanisms underpinning its pathogenesis are not well ***,a multi-omics approach was applied to groupers infected with Singapore grouper iridovirus(SGIV),focusing on the roles of key *** showed that SGIV induced obvious histopathological damage and changes in metabolic enzymes within the ***,SGIV significantly reduced the contents of lipid droplets,triglycerides,cholesterol,and *** analysis indicated that the altered metabolites were enriched in 19 pathways,with a notable down-regulation of lipid metabolites such as glycerophosphates and alpha-linolenic acid(ALA),consistent with disturbed lipid homeostasis in the *** of transcriptomic and metabolomic data revealed that the top enriched pathways were related to cell growth and death and nucleotide,carbohydrate,amino acid,and lipid metabolism,supporting the conclusion that SGIV infection induced liver metabolic *** integrative transcriptomic and proteomic analysis indicated that SGIV infection activated crucial molecular events in a phagosome-immune depression-metabolism dysregulation-necrosis signaling *** note,integrative multi-omics analysis demonstrated the consumption of ALA and linoleic acid(LA)metabolites,and the accumulation of L-glutamic acid(GA),accompanied by alterations in immune,inflammation,and cell death-related *** experimental data showed that ALA,but not GA,suppressed SGIV replication by activating antioxidant and anti-inflammatory responses in the ***,these findings provide a comprehensive resource for understanding host response dynamics during fish iridovirus infection and highlight the antiviral potential of ALA in the prevention and treatment of iridoviral diseases.

关键词： Iridovirus Liver damage Metabolic reprogramming SGIV Alpha-linolenic acid Antiinflammatory

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The IRTI and CPUS double-blind controlled clinical study on DVT

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The IRTI and CPUS double-blind controlled clinical study on ...