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Relationships of growth and mortality to enzymatic activity,and the relative mRNA expression of cultured scallops Patinopecten yessoensis in the Yellow Sea, China

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Journal of Oceanology and Limnology 2019年第4期37卷 1409-1422页

作者： LIU Chao WU Fucun que huayong ZHANG Guofan Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China National & Local Joint Engineering Laboratory of Ecological Mariculture Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

The bottom aquaculture of yesso scallop (Patinopecten yessoensis) has developed rapidly in the north of Yellow Sea, China, but not without accompanying productivity problems. We, therefore, conducted field surveys to investigate factors related to growth and mortality rates of bottom-cultured scallops after release. Specifically, we focused on the effects of total antioxidant capacity (TAOC) and three key enzymes involved in antioxidant and metabolic function:glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LDH), and superoxide dismutase (SOD). Across summer and winter, we also measured the relative mRNA expression of GPT and SOD to understand their seasonal variation in the scallops, along with how such variation correlated to growth and mortality. Results show that bottom cultured scallops experienced mass mortality during the first six months post-release. During winter (December), scallops grew more rapidly and suffered less mortality than in summer (August). The observed lower performance probably resulted from less advantageous environmental factors during the summer seasons, such as high temperature and low dissolved oxygen. These environmental stressors enhance protein consumption while decreasing energetic resources in scallops. Furthermore, scallops in summer exhibited high antioxidant levels that probably competed for energy with process integral to growth and survival. These negative factors combined to elevate mortality rates. In conclusion, we provided evidence suggesting correlations between metabolic/antioxidant activity and growth and mortality of bottom-cultured yesso scallops. These correlations implied us an accurate method to estimate the performance of bottom culture system. Suggestions about innovative aquaculture techniques were also discussed in the study. Our results might provide a possible guideline to the improvement of bottom culture techniques for this commercially valuable seafood species.

关键词： yesso scallop (Patinopecten yessoensis) bottom culture growth and mortality rate antioxidant and metabolic activity relative mRNA expression

The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas

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Journal of Oceanology and Limnology 2019年第4期37卷 1317-1323页

作者： HUANG Wen XU Fei LI Li que huayong ZHANG Guofan Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China School of Marine Sciences Guangxi University Nanning 530004 China University of Chinese Academy of Sciences Beijing 100049 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266237 China Center for Ocean Mega-Science Chinese Academy of Sciences Qingdao 266071 China

Thyroid hormones (THs) are indispensable for each phyla in Chordata, while their functions in the non-chordate invertebrates are indistinct. Studies on the TH system in non-chordate invertebrates are important for understanding the evolution of TH system and may be applied in aquaculture or biofouling control at the same time. Iodothyronine deiodinases are keys to studying the TH system, as they are critical enzymes in maintaining TH homeostasis by catalyzing the initiation and termination of the effects of thyroid hormone in vertebrates. Here, we report the primary physiological effects of T4, the outer ring deiodinase activity, and a similar transcription regulation of two oyster deiodinases by TH receptor (CgTR) in an invertebrate, Pacific oyster Crassostrea gigas. L-thyroxine (T4) may have an important physiological function in the oyster, suggested by the growth retardation effect of excessive T4 in umbo larvae stage. The outer ring deiodinase activity transforming T4 to T3 (3, 3', 5-triiodothyronine) was then detected in the Pacific oyster in vivo, which may be conducted by two oyster deiodinases (CgDx and CgDy). Transcription regulation of CgTR onto these two deiodinase genes was also verified by electrophoretic mobility shift assay and dual luciferase reporter assay in mammalian cells. These results contribute to a better understanding of the evolution of the TH system.

关键词： iodothyronine deiodinase mollusk thyroid hormone (TH) TH responsive elements

De novo transcriptome sequencing reveals candidate genes involved in orange shell coloration of bay scallop Argopecten irradians

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Journal of Oceanology and Limnology 2018年第4期36卷 1408-1416页

作者： TENG Wen CONG Rihao que huayong ZHANG Guofan Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China National & Local Joint Engineering Laboratory of Ecological Mariculture Qingdao 266000 China

Molluscan shell color has received persistent attention for its distinctive diversity and complexity. In the present study, six transcriptome libraries obtained from two developmental stages, pre-pigmentation and post-pigmentation, were used for paired-end sequencing in the bay scallop Argopecten irradians. In total, 289 839 646 paired-end reads were assembled into 70 929 transcripts. Using BLASTX and BLASTN, 30 896 unigenes were successfully annotated against the SWISS-PROT, NR, and KOG database. Gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes classification identified numbers of unigenes involved in biomineralization and pigmentation. Digital gene expression analysis revealed that melanin, trace metal elements and porphyrins are potentially involved in shell coloration of A. irradians.

关键词： molluscs shell coloration differentially expressed genes gene ontology

Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas

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Current Zoology 2013年第1期59卷 109-115页

作者： Xiaotong WANG Xiaorui SONG Tong WANG Qihui ZHU Guoying MIAO Yuanxin CHEN Xiaodong FANG huayong que Li LI Guofan ZHANG Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China BGI-Shenzhen Shenzhen 518083 China

Mollusc shell matrix proteins （SMPs） are important functional components embedded in the shell and play a role in shell formation. A SMP （Pif177） was identified previously from the nacreous layer of the Japanese pearl oyster Pinctadafucata, and its cleavage products （named pfPif97 and pfPif80 proteins） were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue （cgPif97） shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages in- dicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was af- fected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose in- creased, suggesting that the cgPip7 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species [Current Zoology 59 （1）： 109-115, 2013].

关键词： Mollusca Crassostrea gigas Pif97 gene Biornineralization Shell formation Calcite

Microstructure and thermal properties of dissimilar M300–CuCr1Zr alloys by multi-material laser-based powder bed fusion

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International Journal of Minerals,Metallurgy and Materials 2024年第1期31卷 118-128页

作者： Xiaoshuang Li Dmitry Sukhomlinov Zaiqing que Aerosint SA B-4040 HerstalBelgium Aalto University Department of Chemical and Metallurgical EngineeringEspoo 02150Finland VTT Technical Research Centre of Finland Espoo 02044Finland

Multi-material laser-based powder bed fusion (PBF-LB) allows manufacturing of parts with 3-dimensional gradient and additional functionality in a single step. This research focuses on the combination of thermally-conductive CuCr1Zr with hard M300 tool *** interface configurations of M300 on CuCr1Zr and CuCr1Zr on M300 were investigated. Ultra-fine grains form at the interface due to the low mutual solubility of Cu and steel. The material mixing zone size is dependent on the configurations and tunable in the range of0.1–0.3 mm by introducing a separate set of parameters for the interface layers. Microcracks and pores mainly occur in the transition *** of these defects, the thermal diffusivity of bimetallic parts with 50vol% of CuCr1Zr significantly increases by 70%–150%compared to pure M300. The thermal diffusivity of CuCr1Zr and the hardness of M300 steel can be enhanced simultaneously by applying the aging heat treatment.

关键词： multi-material additive manufacturing laser-based powder bed fusion thermal diffusivity dissimilar metals copper alloy

Combustion mechanism of benzene in iron ore sintering process:experimental and simulation

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Journal of Iron and Steel Research International 2024年第1期31卷 195-203页

作者： Zhi-gang que Jin-ming Shi Xian-bin Ai Institute of Energy Research Jiangxi Academy of ScienceNanchang330096JiangxiChina Jiangxi Carbon Neutralization Research Center Nanchang330096JiangxiChina

Benzene is a typical component of volatile organic compounds(VOCs)in the iron ore sintering flue *** combustion behavior of benzene directly affects the emission of VOCs in iron ore sintering *** effects of temperature,benzene,and oxygen concentrations on the conversion ratio of benzene were investigated by experiments and numerical *** experiments were carried out in a tube reactor at temperatures of 773-1098 K,benzene concentrations of 0.01-0.03 vol.%,and oxygen concentrations of 10-21 vol.%.The numerical simulation was performed with the plug flow model in the CHEMKIN program based on a kinetic model that consists of 132 chemical species and 772 elementary step-like *** experimental results reveal that increasing the temperature and benzene concentration could signifi-cantly promote benzene *** is attributed to the increase in the reaction rates of all steps in the pathway for forming CO_(2)and H_(2)*** addition,due to the large equivalent ratio of oxygen to benzene,the conversion ratio of benzene remained constant at different oxygen *** simulation results were in good agreement with the experimental results and indicated that six elementary reactions dominated the formations of CO_(2)and H_(2)*** oxidations of C_(6)H_(5)O,CO,and C_(5)H_(4)O intermediates to CO_(2)were the limiting steps in the reaction pathways.

关键词： Benzene Volatile organic compound Iron ore sinter Combustion Simulation Temperature Oxygen

Fantastic voyage: The journey of NLRP3 inflammasome activation

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Genes & Diseases 2024年第2期11卷 819-829页

作者： Xiangyong que Sihao Zheng Qibin Song Huadong Pei Pingfeng Zhang Cancer Center Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Department of Oncology Georgetown Lombardi Comprehensive Cancer CenterGeorgetown University Medical CenterWashingtonDC 20057USA

NLRP3 inflammasome,an intracellular multiprotein complex,can be activated by a range of pathogenic microbes or endogenous hazardous *** activation results in the release of cytokines such as IL-1β and IL-18,as well as Gasdermin D which eventually causes *** activation of NLRP3 inflammasome is under strict control and regulation by numerous pathways and *** excessive activation can lead to a persistent inflammatory response,which is linked to the onset and progression of severe *** studies have revealed that the subcellular localization of NLRP3 changes significantly during the activation *** this review,we review the current understanding of the molecular mechanism of NLRP3 inflammasome activation,focusing on the subcellular localization of NLRP3 and the associated regulatory *** aim to provide a comprehensive understanding of the dynamic transportation,activation,and degradation processes of NLRP3.

关键词： Inflammasome activation Innate immunity NLRP3 NLRP3 inflammasome Subcellular localization

An improved deviation model for transonic stages in axial compressors

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Chinese Journal of Aeronautics 2024年第7期37卷 93-108页

作者： Xiaochen WANG Xuesong LI Xiaodong REN Chunwei GU Xiaobin que Guoyu ZHOU Department of Energy and Power Engineering Tsinghua UniversityBeijing 100084China China United Gas Turbine Technology Co.Ltd. Beijing 102209China

Deviation model is an important model for through-flow analysis in axial *** analysis in classical deviation models is developed under the assumption of onedimensional flow,which is controlled by the continuity *** consider three-dimensional characteristics in transonic flow,this study proposes an improved theoretical analysis method combining force analysis of the blade-to-blade flow with conventional analysis of the continuity *** of shock structures on transverse force,streamwise velocity and streamline curvature in the blade-to-blade flow are analyzed,and support the analytical modelling of density flow ratio between inlet and outlet ***,a novel deviation model for transonic stages in axial compressors is proposed in this *** empirical coefficients are corrected based on the experimental data of a linear cascade,and the prediction accuracy is validated with the experimental data of a three-stage transonic *** novel model provides accurate predictions for meridional flow fields at the design point and performance curves at design speed,and shows obvious improvements on classical models by Carter and C¸etin.

关键词： Axial compressor Transonic flow Deviation model Through-flow method Aerodynamic performance

Infiltration,runoff,and slope stability behaviors of infinite slope with macropores based on an improved Green–Ampt model

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Journal of Mountain Science 2024年第7期21卷 2220-2235页

作者： LI Shanghui WU Guoxiong que Yun JIANG Zhenliang CHENG Gaoyun College of Intelligent Construction Fuzhou University of International Studies and TradeFuzhou 350202China College of Civil Engineering Chongqing Jiao Tong UniversityChongqing 400074China Chongqing Vocational College of Architectural Engineering Chongqing 400072China College of Civil Engineering Fuzhou UniversityFuzhou 350000China Department of Civil and Environmental Engineering Hong Kong University of Science and TechnologyHong Kong 999077China T.Y.Lin International Engineering Consulting(China)Co.Ltd Chongqing 401120China

Infiltration–runoff–slope instability mechanism of macropore slope under heavy rainfall is *** paper studied its instability mechanism with an improved Green–Ampt(GA)model considering the dual-porosity(i.e.,matrix and macropore)and ponding condition,and proposed the infiltration equations,infiltration–runoff coupled model,and safety factor calculation *** show that the infiltration processes of macropore slope can be divided into three stages,and the proposed model is rational by a comparative *** wetting front depth of the traditional unsaturated slope is 17.2%larger than that of the macropore slope in the early rainfall stage and 27%smaller than that of the macropore slope in the late rainfall ***,macropores benefit the slope stability in the early rainfall but not in the *** flow does not occur initially but becomes pronounced with increasing rainfall *** equal depth of the wetting front in the two domains is regarded as the onset criteria of macropore *** analysis shows that macropore flow is delayed by increasing proportion of macropore domain(ω_(f)),whereas promoted by increasing ratio of saturated permeability coefficients between the two domains(μ).The increasing trend of ponding depth is sharp at first and then grows ***,when rainfall duration is less than 3 h,ωf andμhave no significant effect on the safety factor,whereas it decreases with increasingωf and increases with increasingμunder longer duration(≥3 h).With the increase ofω_(f),the slope maximum instability time advances by 10.5 h,and with the increase ofμ,the slope maximum instability time delays by 3.1 h.

关键词： Macropore slope Green–Ampt infiltration model Equivalent wetting front Ponding response time Slope stability