Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas作者机构：Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China BGI-Shenzhen Shenzhen 518083 China
出 版 物：《Current Zoology》 (动物学报（英文版）)
年 卷 期：2013年第59卷第1期
页 面：109-115页
核心收录：
学科分类：0710[理学-生物学] 090801[农学-水产养殖] 07[理学] 0908[农学-水产] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 071007[理学-遗传学]
基 金：supported by the National Basic Research Program of China (973 Program) 国家自然科学基金 the earmarked fund for Modern AgroIndustry Technology Research System 山东省泰山学者建设工程专项 Taishan Scholars Climb Program of Shandong
主 题：Mollusca Crassostrea gigas Pif97 gene Biornineralization Shell formation Calcite
摘 要：Mollusc shell matrix proteins （SMPs） are important functional components embedded in the shell and play a role in shell formation. A SMP （Pif177） was identified previously from the nacreous layer of the Japanese pearl oyster Pinctadafucata, and its cleavage products （named pfPif97 and pfPif80 proteins） were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue （cgPif97） shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages in- dicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was af- fected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose in- creased, suggesting that the cgPip7 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species [Current Zoology 59 （1）： 109-115, 2013].
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