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A 3D-Bioprinted dual growth factor-releasing intervertebral disc scaffold induces nucleus pulposus and annulus fibrosus reconstruction

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Bioactive Materials 2021年第1期6卷 179-190页

作者： Binbin Sun Meifei Lian Yu Han Xiumei Mo Wenbo Jiang zhiguang qiao Kerong Dai Department of Orthopaedic Surgery Shanghai Key Laboratory of Orthopaedic ImplantsShanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineShanghai200080China Clinical and Translational Research Center for 3D Printing Technology Shanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Department of Prosthodontics Shanghai Ninth People's HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyShanghai200011China State Key Lab for Modification of Chemical Fibers&Polymer Materials College of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghai201620China Department of Orthopaedic Surgery Renji HospitalSouth CampusShanghai Jiao Tong University School of MedicineShanghai201112China

Regeneration of Intervertebral disc(IVD)is a scientific challenge because of the complex structure and composition of tissue,as well as the difficulty in achieving bionic ***,an anatomically correct IVD scaffold composed of biomaterials,cells,and growth factors were fabricated via three-dimensional(3D)bioprinting *** tissue growth factor(CTGF)and transforming growth factor-β3(TGF-β3)were loaded onto polydopamine nanoparticles,which were mixed with bone marrow mesenchymal stem cells(BMSCs)for regenerating and simulating the structure and function of the nucleus pulposus and annular *** vitro experiments confirmed that CTGF and TGF-β3 could be released from the IVD scaffold in a spatially controlled manner,and induced the corresponding BMSCs to differentiate into nucleus pulposus like cells and annulus fibrosus like ***,the fabricated IVD scaffold was implanted into the dorsum subcutaneous of nude *** reconstructed IVD exhibited a zone-specific matrix that displayed the corresponding histological and immunological phenotypes:primarily type II collagen and glycosaminoglycan in the core zone,and type I collagen in the surrounding *** testing results demonstrated that it exhibited good biomechanical function of the reconstructed *** results presented herein reveal the clinical application potential of the dual growth factors-releasing IVD scaffold fabricated via 3D ***,the evaluation in large mammal animal models needs to be further studied.

关键词： Intervertebral disc(IVD) Regenerative medicine 3D bioprinting Mesenchymal stem cells(MSCs) Growth factor(GF)

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High-precision,gelatin-based,hybrid,bilayer scaffolds using melt electro-writing to repair cartilage injury

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Bioactive Materials 2021年第7期6卷 2173-2186页

作者： Yu Han Bo Jia Meifei Lian Binbin Sun Qiang Wu Benlin Sun zhiguang qiao Kerong Dai Department of Orthopedic Surgery Shanghai Key Laboratory of Orthopedic ImplantsShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Clinical and Translational Research Center for 3D Printing Technology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Department of Orthopedics Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai200080China Department of Prosthodontics Shanghai Ninth People’s HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyShanghai200011China Department of Orthopedic Surgery Renji HospitalSouth CampusShanghai Jiao Tong University School of MedicineShanghai201112China

Articular cartilage injury is a common disease in the field of *** cartilage has poor self-repairing ability,medical intervention is *** melt electro-writing(MEW)technology,tissue engineering scaffolds with high porosity and high precision can be ***,ordinary materials,especially natural polymer materials,are difficult to *** this study,gelatin was mixed with poly(lactic-co-glycolic acid)to prepare high-concentration and high-viscosity printer ink,which had good printability and formability.A composite scaffold with full-layer TGF-β1 loading mixed with hydroxyapatite was prepared,and the scaffold was implanted at the cartilage injury site;microfracture surgery was conducted to induce the mesenchyme in the bone *** stem cells thereby promoted the repair of damaged *** summary,this study developed a novel printing method,explored the molding conditions based on MEW printing ink,and constructed a bioactive cartilage repair *** scaffold can use autologous bone marrow mesenchymal stem cells and induce their differentiation to promote cartilage repair.

关键词： Gelatin-based hybrid bilayer scaffolds Melt electro-writing Cartilage injury Bone marrow mesenchymal stem cells

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A prototype RF power source for CSNS/RCS

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Science China(Physics,Mechanics & Astronomy) 2011年第S2期54卷 218-221页

作者： WeiLing Huang (1) Hong Sun (1) HaiQi Zhao (2) Yu Liu (2) YanDong Li (2) zhiguang qiao (2) Xiao Li (1) ChunLin Zhang (1) 1. Accelerator Center Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China2. 23rd Institute of China Aerospace Science and Industry Corporation Beijing 100854 China

A prototype RF power source has been built to supply high RF power to a ferrite-loaded cavity, which is a part of R&D of the Rapid Cycling Synchrotron of China Spallation Neutron Source (CSNS/RCS). A direct fast RF feedback amplifier, a 4:1 impedance transformer and auto tuning grid were locally located to compensate the heavy beam loading of CSNS/RCS. Design and commissioning of the RF power source is discussed here, also with some advice on system improvement.

关键词： RF power source synchrotron beam loading RF feedback amplifier

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A prototype RF power source for CSNS/RCS

A prototype RF power source for CSNS/RCS

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第一届中国粒子加速器会议

作者： HUANG WeiLing SUN Hong ZHAO HaiQi LIU Yu LI YanDong qiao zhiguang LI Xiao ZHANG ChunLin Accelerator Center Institute of High Energy PhysicsChinese Academy of Sciences

A prototype RF power source has been built to supply high RF power to a ferrite-loaded cavity,which is a part of R & D of the Rapid Cycling Synchrotron of China Spallation Neutron Source(CSNS/RCS).A direct fast RF feedback amplifier,a 4:1 impedance transformer and auto tuning grid were locally located to compensate the heavy beam loading of CSNS/*** and commissioning of the RF power source is discussed here,also with some advice on system improvement.

关键词： RF power source synchrotron beam loading RF feedback amplifier

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Unravelling biosynthesis and biodegradation potentials of microbial dark matters in hypersaline lakes

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Environmental Science and Ecotechnology 2024年第4期20卷 177-188页

作者： zhiguang Qiu Yuanyuan Zhu Qing Zhang Xuejiao qiao Rong Mu Zheng Xu Yan Yan Fan Wang Tong Zhang Wei-Qin Zhuang Ke Yu School of Environment and Energy Peking University Shenzhen Graduate SchoolShenzhen518055China AI for Science(AI4S)-Preferred Program Peking UniversityShenzhen518055China Southern University of Sciences and Technology Yantian Hospital Shenzhen518081China Institute of Biomedicine and Biotechnology Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China State Key Laboratory of Isotope Geochemistry CAS Center for Excellence in Deep Earth ScienceGuangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou510640China School of Atmospheric Sciences Sun Yat-sen UniversitySouthern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai519082China Department of Civil Engineering University of Hong Kong999077Hong KongChina Department of Civil and Environmental Engineering Faculty of EngineeringUniversity of AucklandNew Zealand

Biosynthesis and biodegradation of microorganisms critically underpin the development of biotechnology,new drugs and therapies,and environmental ***,most uncultured microbial species along with their metabolic capacities in extreme environments,remain *** we unravel the metabolic potential of microbial dark matters(MDMs)in four deep-inland hypersaline lakes in Xinjiang,*** metagenomic binning,we uncovered a rich diversity of 3030 metagenomeassembled genomes(MAGs)across 82 phyla,revealing a substantial portion,2363 MAGs,as previously unclassified at the genus *** unknown MAGs displayed unique distribution patterns across different lakes,indicating a strong correlation with varied physicochemical *** analysis revealed an extensive array of 9635 biosynthesis gene clusters(BGCs),with a remarkable 9403 being novel,suggesting untapped biotechnological ***,some MAGs from potentially new phyla exhibited a high density of these *** biosynthesis,our study also identified novel biodegradation pathways,including dehalogenation,anaerobic ammonium oxidation(Anammox),and degradation of polycyclic aromatic hydrocarbons(PAHs)and plastics,in previously unknown microbial *** findings significantly enrich our understanding of biosynthesis and biodegradation processes and open new avenues for biotechnological innovation,emphasizing the untapped potential of microbial diversity in hypersaline environments.

关键词： Biosynthesis Biodegradation Hypersaline lakes Metagenome-assembled genomes Microbial dark matters

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Postoperative infection caused by Acinetobacter baumannii misdiagnosed as a free-living amoeba species in a humeral head hemiarthroplasty patient:a case report

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Infectious Diseases of Poverty 2018年第1期7卷 335-343页

作者： Jiaxin Tang Huaimin Zhu Li Cai Tingting Tang Jian Tang Yuehua Sun Ming Liu Kerong Dai zhiguang qiao Chao Yu Shanghai Key Laboratory of Orthopaedic Implants Department of Orthopaedic SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Department of Microbiology and Parasitology Second Military Medical UniversityShanghai 200433China Shanghai Municipal Center for Disease Control&Prevention Shanghai 200336China Medical 3D Printing Innovation Research Center Shanghai Jiao Tong University School of MedicineShanghai 200125China Engineering Research Center of Digital Medicine and Clinical Translation Ministry of EducationShanghai 200030China

Background:Acinetobacter baumannii is ubiquitous,facultative intracellular,and opportunistic bacterial *** unique abilities allow it to survive in a diverse range of environments,including health care settings,leading to nosocomial *** its exceptional ability to develop resistance to multiple antibiotics leaves few drug options for *** has been recognized as a leading cause of nosocomial pneumonia and bacteremia over the *** presentation:In this case,a 73-year-old woman presented with a Neer Group VI proximal humeral *** hours after a successfully performed hemiarthroplasty,she developed continuous *** examination revealed that the vitals were *** and radiographic examinations revealed only elevated procalcitonin *** culture revealed no bacterial or fungal *** treatment and empirical broad-spectrum antibiotic therapy showed no apparent ***:We report a postoperative infection caused by Acinetobacter *** infectious pathogen was identified via molecular DNA sequencing and was initially misidentified as a free-living amoeba species upon microscopic *** patient was mistreated with antiamebic combination *** symptoms persisted for over 4 months and were eventually followed by her death.

关键词： Acinetobacter baumannii Free-living amoebae Hemiarthroplasty Case report

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射孔与下泵生产一次完成工艺

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石油钻采工艺 2005年第1期27卷 49-50,60页

作者：乔金中李志广刘长军常会军孟祥农大港油田集团公司钻采中心天津大港300280 大港油田集团公司作业一区天津大港300280

　油井求产时,按常规工序,射孔与下泵作业分 2次完成,由此引起施工周期长、作业费用高、易井喷等问题。鉴于此,提出了射孔与下泵生产一次完成的技术,并从能实现过泵定位、投棒射孔、地层测试等功能的射采联作泵,射孔联作配套工具,工艺程... 详细信息

　油井求产时,按常规工序,射孔与下泵作业分 2次完成,由此引起施工周期长、作业费用高、易井喷等问题。鉴于此,提出了射孔与下泵生产一次完成的技术,并从能实现过泵定位、投棒射孔、地层测试等功能的射采联作泵,射孔联作配套工具,工艺程序,完井管柱设计 4个方面进行了研究,将射孔与下泵抽油工序合二为一,射孔作业后无需起出管柱,便可进行油井投产。经 132井次的现场应用,工艺先进可行,射孔成功率 100%,平均单井泵效为 51. 2%。实践表明,该工艺可实现射孔、下泵作业一体化,简化施工工序,降低修井成本,并能有效防止井喷事故,避免对地层的二次污染,可大面积推广应用。

关键词：射孔联作油井抽油配套工具完井管柱泵效生产费用成本

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姜黄素减缓老年小鼠的骨丢失

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复旦学报（自然科学版） 2021年第2期60卷 238-243,252页

作者：陈玉凤李晶张东曹勇赵祥龙谢亦璘陆继红任一萍乔之光乔中东上海交通大学生命科学技术学院上海200240 如新(中国)日用品保健品有限公司抗衰老科研中心上海201401 上海交通大学医学院附属仁济医院南院骨科上海201112

骨质疏松症是一种年龄相关性骨骼疾病,近年来随着人口老龄化的加剧,其发病率逐渐上升.在这项研究中,我们采用Micro-CT扫描从骨组织微结构角度证实了小鼠随着年龄的增长,它的骨质会逐渐丢失,同时也观察到姜黄素可以有效地减缓老年小鼠的... 详细信息

骨质疏松症是一种年龄相关性骨骼疾病,近年来随着人口老龄化的加剧,其发病率逐渐上升.在这项研究中,我们采用Micro-CT扫描从骨组织微结构角度证实了小鼠随着年龄的增长,它的骨质会逐渐丢失,同时也观察到姜黄素可以有效地减缓老年小鼠的骨丢失.通过小鼠胫骨切片染色和傅里叶显微红外扫描的方法,我们发现姜黄素可以通过增加胫骨骨小梁数目、提高骨矿盐和碳酸盐取代程度、降低结晶度与胶原交联比来阻止老年小鼠伴随年龄增长发生的骨丢失.结果提示我们姜黄素可能通过改变胫骨组织的微结构与微观成分来调控骨重建过程,它可作为治疗年龄相关性骨质疏松症的潜在有用成分.

关键词：骨质疏松姜黄素小鼠

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Optimized transmission coordination mechanisms for opportunistic routing

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High Technology Letters 2011年第3期17卷 236-244页

作者： Chen Wei Qin zhiguang Liu qiao Jin Jing School of Computer Science ＆ Engineering University of Electronic Science ＆ Technology of China Chengdu 610081 P. R. China

The successful rate of transmission coordination of opportunistic routing （OK） is analyzea systematically, and then two efficient transmission coordination mechanisms （TCMs） are proposed for OR： a batch sliding window-based TCM and a bit map-based TCM, to reduce the total number of packet transmissions and improve end-to-end throughput for OR. The batch sliding window-based TCM avoids the oscillation of the successful rate of transmission coordination by transmitting packets in continuous batch mode so as to improve the average successful rate of transmission coordination efficiently, while the bit map-based TCM improves end-to-end throughput of OR by reducing the overhead of transmission coordination. Exhaustive simulations show that the average end-to-end throughput gains of the two proposed TCMs are 15.4% and 6.4% over the batch map-based TCM, respectively.

关键词： opportunistic routing (OR) batch sliding window transmission coordination multi-hop wireless networks bit map

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Recent progress in the structural study of ion channels as insecticide targets

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Insect Science 2022年第6期29卷 1522-1551页

作者： Hadiatullah Hadiatullah Yongliang Zhang Arthur Samurkas Yunxuan Xie Rajamanikandan Sundarraj Han Zuilhof Jianjun qiao zhiguang Yuchi Tianjin Key Laboratory for Modern Drug Delivery&High-Efficiency Collaborative Innovation Center of Chemical Science and EngineeringSchoolof Pharmaceutical Science and TechnologyTianjin UniversityTianjinChina Department of MolecularPharmacology Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin Laboratory of Organic Chemistry Wageningen University&Research WageningenThe Netherlands Department of Environmental Science Tianjin UniversityTianjinChina Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjinChina

Ion channels,many expressed in insect neural and muscular systems,have drawn huge attention as primary targets of *** the recent technical breakthroughs in structural biology,especially in cryo-electron microscopy(cryo-EM),many new high-resolution structures of ion channel targets,apo or in complex with insecticides,have been solved,shedding light on the molecular mechanism of action of the insecticides and resistance *** structures also provide accurate templates for structure-based insecticide screening and rational *** review summarizes the recent progress in the structural studies of 5 ion channel families:the ryanodine receptor(RyR),the nicotinic acetylcholine receptor(nAChR),the voltage-gated sodium channel(VGSC),the transient receptor potential(TRP)channel,and the ligand-gated chloride channel(LGCC).We address the selectivity of the channel-targeting insecticides by examining the conservation of key coordinating residues revealed by the *** possible resistance mechanisms are proposed based on the locations of the identified resistance mutations on the 3D structures of the target channels and their impacts on the binding of ***,we discuss how to develop“green”insecticides with a novel mode of action based on these high-resolution structures to overcome the resistance.

关键词： cryo-electron microscopy structure crystal structure insecticide ion chan-nel resistance

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