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猪圆环病毒2型cap蛋白单克隆抗体的制备与鉴定

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中国兽医科学 2024年第2期54卷 217-225页

作者：张英婕周文锋邹亚文白一涵蒋一凡湛洋王乃东杨毅杜红梅王东亮湖南农业大学动物医学院兽用蛋白质工程疫苗湖南省重点实验室/兽用疫苗逆向创制湖南省工程研究中心湖南长沙410128 湖南大学生物学院湖南长沙410012 常德市畜牧水产事务中心湖南常德415003

本研究旨在制备猪圆环病毒2型(PcV2)cap蛋白单克隆抗体(m Ab),为PcV2免疫学诊断方法的建立和应用提供特异性抗体。首先通过原核大肠杆菌表达系统表达PcV2 cap蛋白,将纯化后的cap蛋白在体外组装成病毒样颗粒(VLPs),以VLPs作为免疫原免疫B... 详细信息

本研究旨在制备猪圆环病毒2型(PcV2)cap蛋白单克隆抗体(m Ab),为PcV2免疫学诊断方法的建立和应用提供特异性抗体。首先通过原核大肠杆菌表达系统表达PcV2 cap蛋白,将纯化后的cap蛋白在体外组装成病毒样颗粒(VLPs),以VLPs作为免疫原免疫BALB/c小鼠,使用杂交瘤细胞技术筛选能稳定分泌抗PcV2 cap蛋白单克隆抗体的杂交瘤细胞株。进一步制备单抗腹水并纯化,通过Western-blot和IFA方法对单克隆抗体的特异性进行鉴定。采用逐步截取法合成45条覆盖PcV2 cap蛋白全长的短肽,并通过间接ELISA鉴定单克隆抗体识别的表位区域。利用两株mAbs对人工感染PcV2的猪肺脏组织进行免疫组化(IHc)检测鉴定。结果表明,成功筛选两株识别PcV2 cap蛋白单克隆杂交瘤细胞株,分别命名为1A6和1A8,其中1A6重链为Ig G2b亚型,1A8重链为Ig G1亚型,轻链均为κ链,纯化后的抗体效价均达1∶656 100。IFA结果显示,两株mAbs均能与PcV2感染的PK-15细胞发生特异性反应;Western-blot结果显示,两株mAbs均与PcV2 cap蛋白发生特异性反应,且不与PcV3和PcV4 cap蛋白发生交叉反应;所获抗体均识别PcV2 cap蛋白c端211-225 aa肽段;IHc检测结果表明,两株mAbs均能与感染PcV2的临床样品发生特异性免疫反应。本研究成功获得了两株单克隆抗体1A6和1A8,均能识别PcV2 cap蛋白,具有良好的特异性,为PcV2免疫学诊断方法的建立及cap蛋白生物学功能研究提供了重要生物材料。

关键词：猪圆环病毒2型 cap蛋白原核表达系统病毒样颗粒单克隆抗体

Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy

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Bioactive Materials 2022年第3期7卷 541-553页

作者： Yuhan Zhou Ting Ye chengzhi Ye chao Wan Siyue Yuan Yushuai Liu Tianyu Li Fagang jiang Jonathan F.Lovell Honglin Jin Jing chen cancer center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022PR China Department of Pediatrics Renmin Hospital of Wuhan UniversityWuhan430060PR China Department of Ophthalmology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022PR China Department of Biomedical Engineering University at BuffaloState University of New York.BuffaloNew York14260USA college of Biomedicine and Health and college of Life Science and Technology Huazhong Agricultural UniversityWuhan430070China

Autologous tumor cells and cell-derived secretions(cDS)can induce antitumor immune *** conditions in which cells are cultured and treated impact cDS,and cellular insults alter their composition and *** this study,we generated cDS from tumor cells exposed to normal culture conditions,hypoxia,cisplatin,radiotherapy,photodynamic therapy,or hypochlorous acid(HOcl).In vitro HOcl-cDS showed the strongest stimulatory effects on dendritic cells and macrophages compared to cDS generated by hypoxia,cisplatin,radiotherapy or photodynamic *** improve HOcl-cDS activity at the tumor site,we loaded HOcl-cDS into a melittin-encapsulated hydrogel *** injected intratumorally,the HOcl-cDS hydrogel promoted tumor cell death,cytotoxic T lymphocyte infiltration,and tumor-associated macrophage reprogramming towards an M1 *** hydrogel inhibited tumor growth and prolonged the survival of mice bearing B16-F10 ***,hydrogel-delivered HOcl-cDS augmented the antitumor effects of immune checkpoint *** results underscore the importance of the cDS generation method and delivery approach for improving cancer immunotherapy.

关键词： cancer immunotherapy Hydrogel Melittin Anti-PD-1 cell-derived secretions

Enhancement of the surface emission at the fundamental frequency and the transmitted high-order harmonics by pre-structured targets

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High Power Laser Science and Engineering 2019年第2期7卷 111-119页

作者： K.q.Pan D.Yang L.Guo Z.c.Li S.W.Li c.Y.Zheng S.E.jiang B.H.Zhang X.T.He Laser Fusion Research center China Academy cf Engineering PhysicsMianyang 621900.China center for Applied Physics and Technology Peking UniversityBeijing 100871China Institute of Applied Physics and computational Mathematics Beijing 100088China

Laser interaction with an ultra-thin pre-structured target is investigated with the help of both two-dimensional and threedimensional particle-in-cell *** the existence of aperiodic structure on the target surface,the laser seems to penetrate through the target at its fundamental frequency even if the plasma density of the target is much higher than the laser’s relativistically critical *** particle-in-cell simulations show that the transmitted laser energy behind the pre-structured target is increased by about two orders of magnitude compared to that behind the flat *** analyses show that the transmitted energy behind the pre-structured target is actually re-emitted by electron’islands’ formed by the surface plasma waves on the target *** other words,the radiation with the fundamental frequency is actually’surface emission,on the target rear *** the intensity of the component with the fundamental frequency,the intensity of the high-order harmonics behind the pre-structured target is also much enhanced compared to that behind the flat *** enhancement of the high-order harmonics is also related to the surface plasma waves generated on the target surfaces.

关键词： high-order harmonics generation high power laser laser-plasma interaction particle-in-cell simulation

Repurposed benzydamine targeting cDK2 suppresses the growth of esophageal squamous cell carcinoma

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Frontiers of Medicine 2023年第2期17卷 290-303页

作者： Yubing Zhou Xinyu He Yanan jiang Zitong Wang Yin Yu Wenjie Wu chenyang Zhang Jincheng Li Yaping Guo Xinhuan chen Zhicai Liu Jimin Zhao Kangdong Liu Zigang Dong The Pathophysiology Department School of Basic Medical SciencesAcademy of Medical SciencesZhengzhou UniversityZhengzhou 450000China The china-US(Henan)Hormel cancer Institute Zhengzhou 450000China State Key Laboratory of Esophageal cancer Prevention and Treatment Zhengzhou 450000China Provincial cooperative Innovation center for cancer chemoprevention Zhengzhou UniversityZhengzhou 450000China cancer chemoprevention International collaboration Laboratory Zhengzhou 450000China Oncology Department The Tumor Hospital of Linzhou CityLinzhou 456500China

Esophageal squamous cell carcinoma (EScc) is one of the leading causes of cancer death worldwide. It is urgent to develop new drugs to improve the prognosis of EScc patients. Here, we found benzydamine, a locally acting non-steroidal anti-inflammatory drug, had potent cytotoxic effect on EScc cells. Benzydamine could suppress EScc proliferation in vivo and in vitro. In terms of mechanism, cDK2 was identified as a target of benzydamine by molecular docking, pull-down assay and in vitro kinase assay. Specifically, benzydamine inhibited the growth of EScc cells by inhibiting cDK2 activity and affecting downstream phosphorylation of McM2, c-Myc and Rb, resulting in cell cycle arrest. Our study illustrates that benzydamine inhibits the growth of EScc cells by downregulating the cDK2 pathway.

关键词： benzydamine cyclin-dependent kinase 2 patient-derived xenograft esophageal squamous cell carcinoma

IcU气管插管患者口腔黏膜压力性损伤风险列线图模型的构建

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中华现代护理杂志 2024年第13期30卷 1764-1770页

作者：王志伟何小燕陶珍珍蒋洋洋祁进芳李振刚董正惠新疆医科大学护理学院乌鲁木齐830011 青海省第五人民医院肿瘤内二科西宁810007 新疆医科大学第六附属医院护理部乌鲁木齐830063 新疆医科大学第一附属医院重症医学科乌鲁木齐830011

目的探讨IcU气管插管患者口腔黏膜压力性损伤(OMPI)的危险因素,并构建列线图模型。方法采用便利抽样法,选取2023年1—5月新疆医科大学第一附属医院IcU收治的640例经口气管插管患者为研究对象,按照是否发生OMPI分为发生组(n=286)和未发生... 详细信息

目的探讨IcU气管插管患者口腔黏膜压力性损伤(OMPI)的危险因素,并构建列线图模型。方法采用便利抽样法,选取2023年1—5月新疆医科大学第一附属医院IcU收治的640例经口气管插管患者为研究对象,按照是否发生OMPI分为发生组(n=286)和未发生组(n=354)。采用二项Logistic回归分析探讨IcU气管插管患者发生OMPI的危险因素,基于独立危险因素创建风险列线图模型,并通过Bootstrap重复抽样法进行内部验证。结果640例IcU气管插管患者中286例患者发生了OMPI。二项Logistic回归分析结果显示,APAcHEⅡ高、改良Beck口腔评分≥11分、使用镇静药物、俯卧位通气、气管导管留置时间长、氧合指数cU气管插管患者发生OMPI的危险因素(PcU气管插管患者OMPI风险列线图模型,结果显示,IcU气管插管患者OMPI风险列线图模型预测性能(受试者工作特征曲线下面积为0.918,95%置信区间:0.897~0.938)和校准度(χ^(2)值为4.647,P=0.795)良好;当阈值概率处于0~1时,决策曲线显示该模型临床效用较好。结论本研究构建的IcU气管插管患者OMPI风险列线图模型具有良好的校准度和预测性能,可作为筛查高风险患者的有效工具。

关键词：重症监护病房气管插管口腔黏膜压力性损伤列线图

A Spiral Graphene Framework containing Highly Ordered Graphene Microtubes for Polymer composites with Superior Through-Plane Thermal conductivity

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chinese Journal of chemistry 2022年第3期40卷 329-336页

作者： Jinrui Gong Xue Tan qilong Yuan Zhiduo Liu Junfeng Ying Le Lv qingwei Yan Wubo chu chen Xue Jinhong Yu Kazuhito Nishimura Nan jiang cheng-Te Lin Wen Dai Key Laboratory of Marine Materials and Related Technologies Zhejiang Key Laboratory of Marine Materials and Protective TechnologiesNingbo Institute of Materials Technology and Engineering(NIMTE)Chinese Academy of SciencesNingboZhejiang 315201China center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBejjing 100049China centre for quantum Physics Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement(MOE)Beijing Key Lab of Nonophotonics&Ultrafine Optoelectronic SystemsSchool of PhysicsBeijing Institute of TechnologyBeijing 100081China college of Materials Science and Engineering Hunan UniversityChangshaHunan 410082China Advanced Nano-processing Engineering Lob Mechanical Systems EngineeringKogakuin UniversityTokyo 192-0015Japan

As the power density of electronic devices increases,there has been an urgent demand to develop highly conductive polymer composites to address the accompanying thermal management *** to the ultra-high intrinsic thermal conductivity,graphene is considered a very promising filler to improve the thermal conductivity of ***,graphene-based polymer composites prepared by the conventional mixing method generally have limited thermal conductivity,even under high graphene loading,due to the failure to construct efficient heat transfer pathways in the polymer ***,a spiral graphene framework(SGF)containing continuous and highly ordered graphene microtubes was developed based on a modified cVD *** embedding into the epoxy(EP)matrix,the graphene microtubes can act as efficient heat pathways,endowing the SGF/EP composites with a high through-plane thermal conductivity of 1.35 W·m^(-1)·K^(-1) at an ultralow graphene loading of 0.86 wt%.This result gives a thermal conductivity enhancement per 1 wt%filler loading of 710%,significantly outperforming various graphene structures as *** addition,we demonstrated the practical application of the SGF/EP composite as a thermal interface material for efficient thermal man-agement of the light-emitting diode(LED).

关键词： Graphene chemical vapor deposition Polymer Thermal conductivity Thermal management material

多参数Z谱MRI对临床胶质瘤分层诊断可能有更好的作用

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国际医学放射学杂志 2022年第2期 239-240页

作者： c.L.Su S.J.Xu D.L.Lin H.q.He Z.H.chen F.c.Damen 朱能谨

摘要目的利用多重对比Z谱MRI对胶质瘤级别、异柠檬酸脱氢酶（IDH）基因型以及1p/19q共缺失状态进行全面、无创的风险因素分层。方法回顾性研究了113例胶质瘤

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氟吡菌酰胺在铁皮石斛中的残留与膳食风险

浙江农业学报 2024年第7期36卷 1666-1676页

作者：王晓梅骆玉琴赵学平陆兰菲方楠王祥云蒋金花何红梅张昌朋王强中国计量大学生命科学学院浙江杭州310018 浙江省农业科学院农产品质量安全与营养研究所浙江杭州310021

建立一种可同时检测氟吡菌酰胺及其代谢物2-(三氯甲基)苯甲酰胺(BZM)在铁皮石斛(鲜样和干样)中残留量的分析方法,采用盆栽实验探究氟吡菌酰胺和BZM在铁皮石斛上的残留行为,评估氟吡菌酰胺的长期膳食风险。建立的检测方法为样品经乙腈提... 详细信息

建立一种可同时检测氟吡菌酰胺及其代谢物2-(三氯甲基)苯甲酰胺(BZM)在铁皮石斛(鲜样和干样)中残留量的分析方法,采用盆栽实验探究氟吡菌酰胺和BZM在铁皮石斛上的残留行为,评估氟吡菌酰胺的长期膳食风险。建立的检测方法为样品经乙腈提取后,用十八烷基硅烷键合硅胶、N-丙基乙二胺和石墨化碳净化,之后用超高效液相色谱串联质谱法进行检测。在0.001~0.1 mg·L^(-1)范围内,所建立的氟吡菌酰胺和BZM检测方法线性关系良好,决定系数(R^(2))≥0.9981。在0.01、0.1、15 mg·kg^(-1)的添加水平下,氟吡菌酰胺和BZM在铁皮石斛中的回收率分别为85.71%~97.98%、94.14%~112.21%,相对标准偏差分别为0.39%~5.93%、1.22%~6.73%。41.7%的氟吡菌酰胺悬浮剂以推荐剂量(有效成分375.3 g·hm^(-2))或2倍推荐剂量施用1次,氟吡菌酰胺在铁皮石斛鲜茎和干茎上的消解均符合一级动力学方程,消解半衰期为19.20~28.76 d,属于易降解农药。在实验条件下,施药后75 d,氟吡菌酰胺和BZM在铁皮石斛中的残留量分别不超过1.98、0.09 mg·kg^(-1)。经测算,氟吡菌酰胺的全膳食国家估算每日摄入量为0.3806 mg,对应的风险商为60.41%,说明41.7%的氟吡菌酰胺悬浮剂在推荐剂量下施用于铁皮石斛上并不会对人体健康产生不可接受的风险。

关键词：氟吡菌酰胺铁皮石斛超高效液相色谱串联质谱农药残留膳食风险评估

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粮饲双审玉米新品种‘兆玉215’的选育及特征特性分析

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分子植物育种 2024年第4期22卷 1213-1221页

作者：邹成林郑德波韦肖宇谭贤杰蒋维萍韦慧黄爱花韦新兴翟瑞宁黄开健谭华广西壮族自治区农业科学院玉米研究所南宁530007 广西兆和种业有限公司南宁530007

粮饲通用型玉米品种在保障国家粮食安全和促进畜牧业发展方面具有重要意义。为培育适合热带地区种植的粮饲通用型玉米品种,为粮饲通用型玉米品种在广西种植提供参考,本研究以q901为母本,以P1107为父本,选育出玉米新品种‘兆玉215’。广... 详细信息

粮饲通用型玉米品种在保障国家粮食安全和促进畜牧业发展方面具有重要意义。为培育适合热带地区种植的粮饲通用型玉米品种,为粮饲通用型玉米品种在广西种植提供参考,本研究以q901为母本,以P1107为父本,选育出玉米新品种‘兆玉215’。广西普通玉米区域试验表明‘兆玉215’两季平均籽粒产量为8030 kg/hm^(2),较对照品种‘桂单162’增产4.0%;生产试验表明‘兆玉215’两季平均籽粒产量为7740 kg/hm^(2),较对照品种增产6.4%。广西青贮玉米区域试验表明‘兆玉215’两季平均全株生物产量为55420 kg/hm^(2),较对照品种增产4.9%;生产试验表明‘兆玉215’两季平均全株生物产量为49587 kg/hm^(2),较对照品种增产7.4%。‘兆玉215’籽粒品质容重为795 g/L,粗蛋白含量8.50%,粗脂肪含量5.1%,粗淀粉含量72.40%,赖氨酸含量0.21%;全株品质粗蛋白含量7.18%,淀粉含量36.8%,中性洗涤纤维含量49.4%,酸性洗涤纤维含量15.3%,干物质含量30.4%。‘兆玉215’在青贮收获期高抗大斑病和茎腐病,抗小斑病和穗腐病。‘兆玉215’在2020年和2021年分别通过了广西普通玉米品种审定(审定号:桂审玉2020090号)和广西青贮玉米品种审定(审定号:桂审玉2021089号),是广西首批粒用和青贮双审定玉米品种,可作为粮饲通用型玉米品种在广西或其相似生态地区种植。

关键词：粮饲双审玉米兆玉215 选育特性