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Bioactive extracellular matrix scaffolds engineered with proangiogenic proteoglycan mimetics and loaded with endothelial progenitor cells promote neovascularization and diabetic wound healing

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Bioactive Materials 2022年第4期7卷 460-473页

作者： Siqi He Tanaya Walimbe Hongyuan Chen Kewa Gao priyadarsini kumar Yifan Wei Dake Hao Ruiwu Liu Diana L.Farmer Kit S.Lam Jianda Zhou Alyssa Panitch Aijun Wang Department of Burns and Plastic Surgery The Third Xiangya Hospital of Central South UniversityChina Department of Surgery UC DavisUnited States Department of Biomedical Engineering UC DavisUnited States Department of Biochemistry and Molecular Medicine UC DavisUnited States Institute for Pediatric Regenerative Medicine Shriners Hospitals for Children Northern CaliforniaUnited States

Diabetic ischemic wound treatment remains a critical clinical challenge.Neovascularization plays a significant role in wound healing during all stages of the tissue repair process.Strategies that enhance angiogenesis and neovascularization and improve ischemic pathology may promote the healing of poor wounds,particularly diabetic wounds in highly ischemic conditions.We previously identified a cyclic peptide LXW7 that specifically binds to integrinαvβ3 on endothelial progenitor cells(EPCs)and endothelial cells(ECs),activates vascular endothelial growth factor(VEGF)receptors,and promotes EC growth and maturation.In this study,we designed and synthesized a multi-functional pro-angiogenic molecule by grafting LXW7 and collagen-binding peptides(SILY)to a dermatan sulfate(DS)glycosaminoglycan backbone,named LXW7-DS-SILY,and further employed this multi-functional molecule to functionalize collagen-based extracellular matrix(ECM)scaffolds.We confirmed that LXW7-DS-SILY modification significantly promoted EPC attachment and growth on the ECM scaffolds in vitro and supported EPC survival in vivo in the ischemic environment.When applied in an established Zucker Diabetic Fatty(ZDF)rat ischemic skin flap model,LXW7-DS-SILY-functionalized ECM scaffolds loaded with EPCs significantly improved wound healing,enhanced neovascularization and modulated collagen fibrillogenesis in the ischemic environment.Altogether,this study provides a promising novel treatment to accelerate diabetic ischemic wound healing,thereby reducing limb amputation and mortality of diabetic patients.

关键词： Diabetic ischemic wound ECM scaffold Endothelial progenitor cells Neovascularization Wound healing

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Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration

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World Journal of Stem Cells 2020年第2期12卷 123-138页

作者： Kewa Gao Siqi He priyadarsini kumar Diana Farmer Jianda Zhou Aijun Wang Department of Burns and Plastic Surgery The Third Xiangya Hospital of Central South UniversityChangsha 410013Hunan ProvinceChina Surgical Bioengineering Laboratory Department of SurgeryUniversity of California DavisSacramentoCA 95817United States Institute for Pediatric Regenerative Medicine Shriners Hospitals for ChildrenSacramentoCA 95817United States Department of Biomedical Engineering University of California DavisDavisCA 95817United States

BACKGROUND Endothelial colony-forming cells(ECFCs)have been implicated in the process of vascularization,which includes vasculogenesis and angiogenesis.Vasculogenesis is a de novo formation of blood vessels,and is an essential physiological process that occurs during embryonic development and tissue regeneration.Angiogenesis is the growth of new capillaries from pre-existing blood vessels,which is observed both prenatally and postnatally.The placenta is an organ composed of a variety of fetal-derived cells,including ECFCs,and therefore has significant potential as a source of fetal ECFCs for tissue engineering.AIM To investigate the possibility of isolating clonal ECFCs from human early gestation chorionic villi(CV-ECFCs)of the placenta,and assess their potential for tissue engineering.METHODS The early gestation chorionic villus tissue was dissociated by enzyme digestion.Cells expressing CD31 were selected using magnetic-activated cell sorting,and plated in endothelial-specific growth medium.After 2-3 wks in culture,colonies displaying cobblestone-like morphology were manually picked using cloning cylinders.We characterized CV-ECFCs by flow cytometry,immunophenotyping,tube formation assay,and Dil-Ac-LDL uptake assay.Viral transduction of CVECFCs was performed using a Luciferase/tdTomato-containing lentiviral vector,and transduction efficiency was tested by fluorescent microscopy and flow cytometry.Compatibility of CV-ECFCs with a delivery vehicle was determined using an FDA approved,small intestinal submucosa extracellular matrix scaffold.RESULTS After four passages in 6-8 wks of culture,we obtained a total number of 1.8×107 CV-ECFCs using 100 mg of early gestational chorionic villus tissue.Immunophenotypic analyses by flow cytometry demonstrated that CV-ECFCs highly expressed the endothelial markers CD31,CD144,CD146,CD105,CD309,only partially expressed CD34,and did not express CD45 and CD90.CV-ECFCs were capable of acetylated low-density lipoprotein uptake and tube formation,similar to cord blood-derived ECFCs(

关键词： Placenta Endothelial colony forming cells Chorionic villi Angiogenesis Tissue engineering

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Exosomal microRNAs from mesenchymal stem/stromal cells: Biology and applications in neuroprotection

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World Journal of Stem Cells 2021年第7期13卷 776-794页

作者： Aida Nasirishargh priyadarsini kumar Lalithasri Ramasubramanian Kaitlin Clark Dake Hao Sabrina V Lazar Aijun Wang Surgical Bioengineering Laboratory Department of SurgeryUniversity of CaliforniaDavis School of MedicineSacramentoCA 95817United States Institute for Pediatric Regenerative Medicine Shriners Hospitals for ChildrenSacramentoCA 95817United States Department of Biomedical Engineering University of California DavisDavisCA 95616United States

Mesenchymal stem/stromal cells(MSCs)are extensively studied as cell-therapy agents for neurological diseases.Recent studies consider exosomes secreted by MSCs as important mediators for MSCs’neuroprotective functions.Exosomes transfer functional molecules including proteins,lipids,metabolites,DNAs,and coding and non-coding RNAs from MSCs to their target cells.Emerging evidence shows that exosomal microRNAs(miRNAs)play a key role in the neuroprotective properties of these exosomes by targeting several genes and regulating various biological processes.Multiple exosomal miRNAs have been identified to have neuroprotective effects by promoting neurogenesis,neurite remodeling and survival,and neuroplasticity.Thus,exosomal miRNAs have significant therapeutic potential for neurological disorders such as stroke,traumatic brain injury,and neuroinflammatory or neurodegenerative diseases and disorders.This review discusses the neuroprotective effects of selected miRNAs(miR-21,miR-17-92,miR-133,miR-138,miR-124,miR-30,miR146a,and miR-29b)and explores their mechanisms of action and applications for the treatment of various neurological disease and disorders.It also provides an overview of state-of-the-art bioengineering approaches for isolating exosomes,optimizing their yield and manipulating the miRNA content of their cargo to improve their therapeutic potential.

关键词： Mesenchymal stromal cells Mesenchymal stromal cell-derived exosomes Exosomal microRNAs Neuroprotective effect

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Securing 3D Point and Mesh Fog Data Using Novel Chaotic Cat Map

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Computers, Materials & Continua 2023年第3期74卷 6703-6717页

作者： K.priyadarsini Arun kumar Sivaraman Abdul Quadir Md Areej Malibari Department of Data Science and Business Systems School of ComputingCollege of Engineering and TechnologySRM Institute of Science and TechnologyKattankulathurChennai603203India School of Computer Science and Engineering Vellore Institute of TechnologyChennai600127India Department of Industrial and Systems Engineering College of EngineeringPrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh11671Saudi Arabia

With the rapid evolution of Internet technology,fog computing has taken a major role in managing large amounts of data.The major concerns in this domain are security and privacy.Therefore,attaining a reliable level of confidentiality in the fog computing environment is a pivotal task.Among different types of data stored in the fog,the 3D point and mesh fog data are increasingly popular in recent days,due to the growth of 3D modelling and 3D printing technologies.Hence,in this research,we propose a novel scheme for preserving the privacy of 3D point and mesh fog data.Chaotic Cat mapbased data encryption is a recently trending research area due to its unique properties like pseudo-randomness,deterministic nature,sensitivity to initial conditions,ergodicity,etc.To boost encryption efficiency significantly,in this work,we propose a novel Chaotic Cat map.The sequence generated by this map is used to transform the coordinates of the fog data.The improved range of the proposed map is depicted using bifurcation analysis.The quality of the proposed Chaotic Cat map is also analyzed using metrics like Lyapunov exponent and approximate entropy.We also demonstrate the performance of the proposed encryption framework using attacks like brute-force attack and statistical attack.The experimental results clearly depict that the proposed framework produces the best results compared to the previous works in the literature.

关键词： Chaotic cat map fog computing encryption 3D point fog 3D mesh

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Surveillance of multidrug resistant uropathogenic bacteria in hospitalized patients in Indian

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Asian Pacific Journal of Tropical Biomedicine 2013年第4期3卷 315-324页

作者： Monali priyadarsini Mishra Nagen kumar Debata Rabindra Nath Padhy Department of Microbiology IMS & Sum HospitalSiksha 'O' Anusandhan UniversityKalinga Nagar.Bhubaneswar751003OdishaIndia Central Research Laboratory IMS & Sum HospitalSiksha 'O' Anusandhan UniversityKalinga Nagar.Bhubaneswar751003OdishaIndia

Objective:To record surveillance,antibiotic resistance of uropathogens of hospitalized patients over a period of 18 months.Methods:Urine samples from wards and cabins were used for isolating urinary tract infection(UTI)-causing bacteria that were cultured on suitable selective media and identified by biochemical tests;and their antibiograms were ascertained by Kirby-Bauer's disc diffusion method,in each 6-month interval of the study period,using 18 antibiotics of five different classes.Results:From wards and cabins,1 245 samples were collected,from which 996 strains of bacteria belonging to 11 species were isolated,during April 2011 to September2012.Two Gram-positive,Staphylococcus aureus(S.aureus)and Enterococcus faecalis(E.faecalis),and nine Gram-negative bacteria,Acinetobacter baumannii,Citrobactcr sp.,Escherichia coli,Enterobacter aerogenes,Klebsiella pneumoniae.Klebsiella oxytoca,Proteus mirabilis,Proteus vulgaris and Pseudomonas aeruginosa were isolated.Both S.aureus and E.faecalis were vancomycin resistant,and resistant-strains of all pathogens increased in each 6-month period of study.Particularly,all Gram-negatives were resistant to nitrofurantoin and co-trimoxazole,the most preferred antibiotics of empiric therapy for UTI.Conclusions:Antibiograms of 11 UTI-causing bacteria recorded in this study indicated moderately higher numbers of strains resistant to each antibiotic studied,generating the fear of precipitating fervent episodes in public health particularly with bacteria,Acinetobacter baumannii,Escherichia coli,Klebsiella pneumoniae and S.aureus.Moreover,vancomycin resistance in strains of S.aureus and E.faecalis is a matter of concern.

关键词： UTI MDR bacteria Hospitalized patients Antibiograms Nosocomial infections Antibiotics Staphylococcus aureus Acinetobacter baumannii Escherichia coli Klebsiella pneumoniae

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Prevalence and antimicrobial susceptibility patterns of bacteria in ICU patients with lower respiratory tract infection: A cross-sectional study

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Journal of Acute Disease 2020年第4期9卷 157-160页

作者： Birasen Behera Kundan kumar Sahu priyadarsini Bhoi Jatindra Nath Mohanty Department of Microbiology IMS and SUM HospitalSiksha“O”Anusandhan University(Deemed to be)K8Kalinga NagarBhubaneswar-751003OdishaIndia Medical Research Laboratory IMS and SUM HospitalSiksha“O”Anusandhan University(Deemed to be)K8Kalinga NagarBhubaneswar-751003OdishaIndia

Objective: To investigate the prevalence of isolated organisms in patients with lower respiratory tract infections and the antibiotic susceptibilities at a tertiary care center. Methods: In this observational and cross-sectional analysis, 114 patients admitted in the intensive care unit were enrolled. The endotracheal aspirates and bronchoalveolar lavage were collected. The bacteria were isolated and identified, and finally, antimicrobial sensitive pattern of the isolated bacteria was examined. Results: The prevalence of infection was 72.72% in male patients and 27.28% in females. The predominant bacteria were Klebsiella pneumoniae (37.50%) followed by Acinetobacter spp. (36.36%), Pseudomonas aeruginosa (7.95%),Escherichia coli (6.81%), Proteus mirabilis (2.27%), atypical Escherichia coli (1.13%), Enterococcus spp. (1.13%),Elizabethkingia meningoseptica (1.13%),Staphylococcus aureus (1.13%),Proteus vulgaris (1.13%), Citrobacter freundii (1.13%), and Citrobacter koseri (1.13%). High resistance to cephalosporins (82.18%) was demonstrated in all Gram-negative bacteria. Bacteria showed susceptibility to colistin (88.75%) followed by tigecycline (83.11%), gentamycin (36.18%), and amikacin (49.23%). Conclusions: As the most frequent respiratory organisms, Klebsiella pneumoniae and Acinetobacter spp. have increased resistance to cephalosporins and susceptibility to colistin followed by tigecycline.

关键词： Antimicrobial susceptibility Lower respiratory tract infection Klebsiella pneumonia Cephalosporins

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