Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration

作     者：Kewa Gao Siqi He Priyadarsini Kumar Diana Farmer Jianda Zhou Aijun Wang 

作者机构：Department of Burns and Plastic SurgeryThe Third Xiangya Hospital of Central South UniversityChangsha 410013Hunan ProvinceChina Surgical Bioengineering LaboratoryDepartment of SurgeryUniversity of California DavisSacramentoCA 95817United States Institute for Pediatric Regenerative MedicineShriners Hospitals for ChildrenSacramentoCA 95817United States Department of Biomedical EngineeringUniversity of California DavisDavisCA 95817United States 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2020年第12卷第2期

页      面：123-138页

核心收录：

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：the Shriners Hospital for Children Postdoctoral Research Fellowship award,No.84704-NCA-19 UC Davis School of Medicine Dean’s Fellowship award and funding from the NIH,No.5R01NS100761-02 and No.R03HD091601-01 the California Institute of Regenerative Medicine,No.PC1-08103 and No.CLIN1-11404 Shriners Hospitals for Children,No.85120-NCA-16,No.85119-NCA-18,No.85108-NCA-19 and No.87200-NCA-19 March of Dimes Foundation,No.5FY1682 

主　　题：Placenta Endothelial colony forming cells Chorionic villi Angiogenesis Tissue engineering 

摘      要：BACKGROUND Endothelial colony-forming cells(ECFCs)have been implicated in the process of vascularization,which includes vasculogenesis and *** is a de novo formation of blood vessels,and is an essential physiological process that occurs during embryonic development and tissue *** is the growth of new capillaries from pre-existing blood vessels,which is observed both prenatally and *** placenta is an organ composed of a variety of fetal-derived cells,including ECFCs,and therefore has significant potential as a source of fetal ECFCs for tissue *** To investigate the possibility of isolating clonal ECFCs from human early gestation chorionic villi(CV-ECFCs)of the placenta,and assess their potential for tissue *** The early gestation chorionic villus tissue was dissociated by enzyme *** expressing CD31 were selected using magnetic-activated cell sorting,and plated in endothelial-specific growth *** 2-3 wks in culture,colonies displaying cobblestone-like morphology were manually picked using cloning *** characterized CV-ECFCs by flow cytometry,immunophenotyping,tube formation assay,and Dil-Ac-LDL uptake *** transduction of CVECFCs was performed using a Luciferase/tdTomato-containing lentiviral vector,and transduction efficiency was tested by fluorescent microscopy and flow *** of CV-ECFCs with a delivery vehicle was determined using an FDA approved,small intestinal submucosa extracellular matrix *** After four passages in 6-8 wks of culture,we obtained a total number of 1.8×107 CV-ECFCs using 100 mg of early gestational chorionic villus *** analyses by flow cytometry demonstrated that CV-ECFCs highly expressed the endothelial markers CD31,CD144,CD146,CD105,CD309,only partially expressed CD34,and did not express CD45 and ***-ECFCs were capable of acetylated low-density lipoprotein uptake and tub