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The structure of microbial populations in nelore GIT reveals inter-dependency of methanogens in feces and rumen

Journal of Animal science and Biotechnology 2020年第2期11卷 577-586页

作者： Bruno G.n.Andrade Flavia A.Bressani Rafael R.C.Cuadrat Polyana C.Tizioto priscila s.n.de oliveira Gerson B.Mourão Luiz L.Coutinho James M.Reecy James E.Koltes Paul Walsh Alexandre Berndt Julio C.P.Palhares Luciana C.A.Regitano Embrapa southeast Livestock São CarlosBrazil department of Molecular Epidemiology German Institute of Human Nutrition Potsdam-Rehbrücke(DIfE)NuthetalGermany nGs Genomic solutions PiracicabaBrazil department of Animal science University of São Paulo/ESALQPiracicabaBrazil department of Animal science Iowa State UniversityAmesIAUSA nsilico Life science DublinIreland

Background:The success of different species of ruminants in the colonization of a diverse range of environments is due to their ability to digest and absorb nutrients from cellulose,a complex polysaccharide found in leaves and *** rely on a complex and diverse microbial community,or microbiota,in a unique compartment known as the rumen to break down this *** in microbial populations of the rumen can affect the host’s development,health,and ***,accessing the rumen is stressful for the ***,the development and use of alternative sampling methods are needed if this technique is to be routinely used in cattle *** this end,we tested if the fecal microbiome could be used as a proxy for the rumen microbiome due to its *** investigated the taxonomic composition,diversity and inter-relations of two different GIT compartments,rumen and feces,of 26 nelore(Bos indicus)bulls,using next Generation sequencing(nGs)metabarcoding of bacteria,archaea and ciliate ***:We identified 4265 Amplicon sequence Variants(AsVs)from bacteria,571 from archaea,and 107 from protozoa,of which 143(96 bacteria and 47 archaea)were found common between both *** most prominent bacterial phyla identified were Bacteroidetes(41.48%)and Firmicutes(56.86%)in the ruminal and fecal microbiomes,respectively,with Prevotella and Ruminococcaceae UCG-005 the most relatively abundant genera identified in each *** most abundant archaeal phylum identified was Euryarchaeota,of which Methanobrevibacter gottschalkii,a methanogen,was the prevalent archaeal species identified in both *** were found exclusively identified in the rumen with Bozasella/Triplumaria being the most frequent genus ***-occurrence among ruminal and fecal AsVs reinforces the relationship of microorganisms within a biological ***,the co-occurrence of shared archaeal AsVs between microbiomes indicat

关键词： Archaea Bacteria Bos indicus Metabarcoding Methanobrevibacter Microbiome Microbiota

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Update on the use of 45s5 bioactive glass in the treatment of bone defects in regenerative medicine

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World Journal of Orthopedics 2024年第3期15卷 204-214页

作者： Dayane Maria Braz nogueira Marcelie priscila de oliveira Rosso Daniela Vieira Buchaim Mariana schutzer Ragghianti Zangrando Rogério Leone Buchaim department of Prosthodontics and Periodontics Bauru School of DentistryUniversity of São PauloBauru 17012-901Brazil department of Biological sciences Bauru School of DentistryUniversity of São PauloBauru 17012-901Brazil Medical school University Center of AdamantinaAdamantina 17800-000Brazil Postgraduate Program in structural and Functional Interactions in Rehabilitation Postgraduate DepartmentUniversity of MaríliaMarília 17525-902Brazil

Bone regeneration is a critical area in regenerative medicine,particularly in orthopedics,demanding effective biomedical materials for treating bone defects.45s5 bioactive glass(45s5 BG)is a promising material because of its osteoconductive and bioactive *** research in this field continues to advance,keeping up-to-date on the latest and most successful applications of this material is *** achieve this,we conducted a comprehensive search on Pub-Med/MEDLInE,focusing on English articles published in the last *** search used the keywords“bioglass 45s5 AnD bone defect”in *** found 27 articles,and after applying the inclusion criteria,we selected 15 studies for detailed *** of these studies compared 45s5 BG with other cement or scaffold *** comparisons demonstrate that the addition of various composites enhances cellular biocompatibility,as evidenced by the cells and their osteogenic ***,the use of 45s5 BG is enhanced by its antimicrobial properties,opening avenues for additional investigations and applications of this biomaterial.

关键词： Biocompatible materials Bioglass Bone regeneration

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Maternal supplementation with n-3 fatty acids affects placental lipid metabolism, inflammation, oxidative stress, the endocannabinoid system, and the neonate cytokine concentrations in dairy cows

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Journal of Animal science and Biotechnology 2024年第5期15卷 2012-2030页

作者： priscila dos santos silva Gitit Kra Yana Butenko Jayasimha Rayalu Daddam Yishai Levin Maya Zachut department of Ruminant science Institute of Animal SciencesARO Volcani InstituteRishon LeZionIsrael department of Animal science The Robert H.Smith Faculty of AgricultureFood and EnvironmentThe Hebrew University of JerusalemRehovotIsrael department of Animal sciences Michigan State UniversityEast LansingMIUSA The nancy and stephen Grand Israel national Center for Personalized Medicine Weizmann Institute of ScienceRehovotIsrael

Background The placenta plays a crucial role in supporting and influencing fetal *** compared the effects of prepartum supplementation with omega-3(n-3)fatty acid(FA)sources,flaxseed oil(FLX)and fish oil(FO),on the expression of genes and proteins related to lipid metabolism,inflammation,oxidative stress,and the endocannabinoid system(ECs)in the expelled placenta,as well as on FA profile and inflammatory response of ***-pregnant Holstein dairy cows were supplemented with saturated fat(CTL),FLX,or *** cotyledons(n=5)were collected immediately after expulsion,and extracted RnA and proteins were analyzed by RTPCR and proteomic *** blood was assessed for FA composition and concentrations of inflammatory *** FO increased the gene expression of fatty acid binding protein 4(FABP4),interleukin 10(IL-10),catalase(CAT),cannabinoid receptor 1(CnR1),and cannabinoid receptor 2(CnR2)compared with CTL *** expression of ECs-enzyme FA-amide hydrolase(FAAH)was lower in FLX and FO than in *** analysis identified 3,974 proteins;of these,51–59 were differentially abundant between treatments(P≤0.05,|fold change|≥1.5).Top canonical pathways enriched in FLX *** and in FO *** were triglyceride metabolism and inflammatory *** n-3 FA increased the placental abundance of FA binding proteins(FABPs)3 and *** abundance of CnR1 cannabinoid-receptor-interacting-protein-1(CnRIP1)was reduced in FO *** silico modeling affirmed that bovine FABPs bind to *** FLX increased the abundance of inflammatory CD44-antigen and secreted-phosphoprotein-1,whereas prostaglandin-endoperoxide synthase 2 was decreased in FO *** *** FO enriched neonatal plasma with n-3 FAs,and both FLX and FO reduced interleukin-6 concentrations compared with *** Maternal n-3 FA from FLX and FO differentially affected the bovine placenta;both enhanced lipid metabolism and modulated oxidative s

关键词： Antioxidants Dairy cows Endocannabinoid system Inflammation Omega-3 fatty acids Placenta

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dehydrated natural Feed for Cats: Influence on Apparent Digestibility Coefficient, Feces scores, and serum Immune, Antioxidant, and Metabolic Biomarkers

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Food and nutrition sciences 2024年第7期15卷 594-611页

作者： Bruno Giorgio de oliveira Cécere Guilherme Luiz deolindo Bianca F. Bissacotti priscila Marquezin Copetti Maria Rosa Chitolina schetinger Bruna Klein Matheus delamea Baldissera Thiago Pereira Ribeiro Walter Hugo Cuelho suarez Daniel Pigatto Monteiro Aleksandro schafer Da silva Graduate Multicenter Program in Biochemistry and Molecular Biology University of the State of Santa Catarina Lages Brazil department of Biochemistry and Molecular Biology Universidade Federal de Santa Maria (UFSM) Santa Maria Brazil department of Animal science Universidade Federal de Santa Maria (UFSM) Chapec Brazil department of Health science Universidade Franciscana (UFN) Santa Maria Brazil department of Animal nutrition Tectron-Tecnologia e Inovao Toledo Brazil

The present study aimed to determine whether natural dehydrated feed (DnF) can replace dry extruded feed while preserving the required daily intake, nutrient digestibility and benefits for immunity and antioxidant response in cats. The animals were divided into two groups of seven animals each. One group received DnF, and the other consumed commercial extruded dry feed (DCF). The experiment lasted 45 days and we measured daily consumption and collected blood and feces samples. The animals in the DnF group showed greater digestibility of fiber and mineral matter than DCF. Cats in the DnF group had a higher volume of feces. DnF cats had lower leukocyte and granulocyte counts and cholesterol levels. serum enzymatic antioxidant responses and non-enzymatic were higher in DnF cats. Cats in the DnF group showed lower serum acute phase proteins in addition to a tendency toward a higher concentration of transferrin than in the DCF group. Fecal Escherichia coli and total coliform counts of cats fed DnF were higher than DCF. We conclude that the daily consumption of cats is adequate. This feed had greater digestibility of fiber and mineral matter, in addition to an improvement in antioxidant and immunological responses.

关键词： Alternative Feed Antioxidant Companion Animals Health

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Effect of silymarin on biochemical indicators in patients with liver disease: systematic review with meta-analysis

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World Journal of Gastroenterology 2017年第27期23卷 5004-5017页

作者： Camila Ribeiro de Avelar Emile Miranda Pereira priscila Ribas de Farias Costa Rosangela Passos de Jesus Lucivalda Pereira Magalhaes de oliveira Postgraduation Program Federal University of Bahia departament of science nutrition Federal University of Bahia

To evaluate the effect of silymarin on the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AsT) and gamma glutamyl transpeptidase (γGT) in patients with liver diseases. METHODsA systematic review with meta-analysis of ramdomized and controlled clinical trials was performed, evaluating the effects of sylimarin in patients with hepatic diseases, published by January 31, 2016. Clinical trials were sought on the basis of The Cochrane Central Register of Controlled Trials in the Cochrane Library, PubMed/Medline, scopus, Web of science, Lilacs and Clinical Trials. The trials with adult and elderly patients of both sexes, with Liver Diseases who took oral silymarin supplementation, as extract or isolated, as well as silymarin combined with other nutrients, were included. The trials should provide information about the intervention, such as dosages and detailing of the product used, besides the mean and standard deviation of serum levels of ALT, AsT and γGT of the baseline and at the end of the intervention. REsULTsAn amount of 10904 publications were identified. From those, only 17 were included in the systematic review and 6 in the meta-analysis, according to the used selection criteria. In this meta-analysis, the results indicated a reduction of 0.26 IU/mL (95%CI: -0.46-0.07, P = 0.007) at the level of ALT and 0.53 IU/mL (95%CI: -0.74-0.32, P = 0.000) at the serum levels of AsT after using the silymarin, both, statistically significant, but with no clinical relevance. There was no significant change in the γGT levels. subgroup analyzes were also performed for the biochemical markers in relation to the type of intervention, whether silymarin isolated or associated with other nutrients and the time of intervention (whether ≥ 6 mo or significant differences were not found. The evaluated studies presented a high degree of heterogeneity and low methodological quality in the carried out analysis. COnCLUsIOnsilymarin minimally reduced, but w

关键词： systematic review Liver diseases Milk thistle silymarin Meta-analysis

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Buzz-pollination in neotropical bees:genus-dependent frequencies and lack of optimal frequency for pollen release

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Insect science 2020年第1期27卷 133-142页

作者： Conrado Augusto Rosi-denadai priscila Cassia souza Araujo Lucio Antonio de oliveira Campos Lirio Cosme Jr. Raul narciso Carvalho Guedes departamento de Entomologia Universidade Federal de VigosaVigosaMinas GeraisBrazil departamento de Biologia Geral Universidade Federal de VigosaVigosaMinas GeraisBrazil

Over 50 genera of bees release pollen from flower anthers using thoracic vibrations,a phenomenon known as *** efficiency of this process is directly affected by the mechanical properties of the buzzes,namely the duration,amplitude,and ***,although the effects of the former two properties are well described,the role of buzz frequency on pollen release remains ***,nearly all of the existing studies describing vibrational properties of natural buzz-pollination are limited to bumblebees(Bombus)and carpenter bees(Xvlocopa)constraining our current understanding of this behavior and its ***,we attempted to minimize this shortcoming by testing whether flower anthers exhibit optimal frequency for pollen release and whether bees tune their buzzes to match these(optimal)*** true,certain frequencies will trigger more pollen release and lighter bees will reach buzz frequencies closer to this optimum to compensate their smaller buzz *** strategies were used to test these hypotheses:(i)the use of(artificial)vibrational playbacks in a broad range of buzz frequencies and amplitudes to assess pollen release by tomato plants(solarium Ivcopersicum L.)and(ii)the recording of natural buzzes of neotropical bees visiting tomato plants during *** playback experiment indicates that although buzz frequency does affect pollen release,no optimal frequency exists for *** addition,the recorded results of natural buzz-pollination reveal that buzz frequencies vary with bee genera and are not correlated with body ***,neither bees nor plants are tuned to optimal pollen release *** frequency of buzz-pollination is a likely consequence of the insect flight machinery adapted to reach higher accelerations,while flower plant response to buzz-pollination is the likely result of its pollen granular properties.

关键词： buzz amplitude buzz frequency floral sonication pollination stingless bees vibration

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Black,pinto and white beans lower hepatic lipids in hamsters fed high fat diets by excretion of bile acids

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食物生产加工与营养(英文) 2020年第1期2卷 249-257页

作者： priscila L.s.Alves Jose de J Berrios James Pan Wallace H.Yokoyama Agricultural Research service United States Department of AgricultureHealthy Processed Foods ResearchWestern Regional Research Center800 Buchanan St.AlbanyCA 94710USA

Hypolipidemic and anti-obesity properties of extruded black,pinto and white beans were examined in male syrian hamsters fed high fat diets for 3 *** efficiency and effectiveness of extruder processing to eliminate heatlabile antinutrients were also *** fed a high fat diet containing 40%extruded black beans gained the same amount of weight as animals on a low-fat chow diet(based on AIn-93G).Total plasma cholesterol of the hamsters fed bean flour-based diets tended to be lower,ranging between 298 and 356 mg/dL,compared to 365 mg/dL for the control group and total and free liver cholesterol was about 50 and 33%lower,respectively,in the livers(freeze-dried)of hamsters fed the black turtle bean(BB)and pinto bean(PB)*** nitrogen content of the feces from hamsters fed the PB and white bean(WB)diets were 2.6x higher than the control and the excretion of deoxycholic acid(DCA)and lithocholic acid(LCA)was higher in all legume fed hamsters The results show that extrusion efficiently and economically reduces anti-nutritive factors that inhibit the digestion and absorption of proteins and carbohydrates.

关键词： Beans Extrusion Anti-obesity Bile acid Mice

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Genomic regions and pathways associated with gastrointestinal parasites resistance in santa Inês breed adapted to tropical climate

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Journal of Animal science and Biotechnology 2018年第1期9卷 35-50页

作者： Mariana Piatto Berton Rafael Medeiros de oliveira silva Elisa Peripolli nedenia Bonvino stafuzza Jesús Fernández Martin Maria saura álvarez Beatriz Villanueva Gavina Miguel Angel Toro Georgget Banchero priscila silva oliveira Joanir Pereira Eler Fernando Baldi José Bento sterman Ferraz departamento de Zootecnia Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual PaulistaVia de acesso Prof.Paulo Donato Castellane sno Jaboticabal SP CEP 14884 900 Brazil Instituto nacional de Investigación y Tecnología Agraria y Alimentaria InIA Crta.de la Coruna km75 28040 Madrid Spain departamento de Producción Agraria School of AgriculturalFood and Byosystems EngineeringUniversisdad Politécnica de MadridCampus Ciudad Universitaria Avda.Complutense 3-Avda.Puerta Hierro 28040 Madrid Spain Instituto nacional de Investigación Agropecuária(InIA) Ruta 50 Kin. 12 Colonia Uruguay Faculdade de Zootecnia e Engenharia de Alimentos Nucleo de Apoio à Pesquisa em Melhoramento AnimalBiotecnologia e TransgeniaUniversidade de Sao Paulo Rua Duque de Caxias Norte 225 Pirassununga SP CEP 13635 900Brazil

Background： The aim of this study was to estimate variance components and to identify genomic regions and pathways associated with resistance to gastrointestinal parasites, particularly Haemonchus contortus, in a breed of sheep adapted to tropical climate. Phenotypes evaluations were performed to verify resistance to gastrointestinal parasites, and were divided into two categories： i） farm phenotypes, assessing body condition score（BCs）, degree of anemia assessed by the famacha chart（FAM）, fur score（Fs） and feces consistency（FC）; and ii） lab phenotypes,comprising blood analyses for hematocrit（HCT）, white blood cell count（WBC）, red blood cell count（RBC）,hemoglobin（HGB）, platelets（PLT） and transformed（log10） egg per gram of feces（EPG_（log））. A total of 576 animals were genotyped with the Ovine snP12k Bead Chip（Illumina, Inc.）, that contains 12,785 bialleleic snP markers. The variance components were estimated using a single trait model by single step genomic BLUP ***： The overall linkage disequilibrium（LD） mean between pairs of markers measured by r2 was 0.23. The overall LD mean between markers considering windows up to 10 Mb was 0.07. The mean LD between adjacent snPs across autosomes ranged from 0.02 to 0.10. Heritability estimates were low for EPG_（log）（0.11）, moderate for RBC（0.18）, PLT（0.17） HCT（0.20）, HGB（0.16） and WBC（0.22）, and high for FAM（0.35）. A total of 22, 21, 23, 20, 26, 25 and 23 windows for EPG_（log）for FAM, WBC, RBC, PLT, HCT and HGB traits were identified, respectively. Among the associated windows, 10 were shown to be common to HCT and HGB traits on OAR1, OAR2, OAR3, OAR5, OAR8 and ***： The traits indicating gastrointestinal parasites resistance presented an adequate genetic variability to respond to selection in santa Inês breed, and it is expected a higher genetic gain for FAM trait when compared to the others. The level of LD estimated for markers separated by less than 1 Mb indicated that the Ovine snP12k Bead Chip m

关键词： Gwas Linkage disequilibrium arasites resistance santa Inês breed

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delayed spontaneous reversibility of left bundle branch block in non-ischemic cardiomyopathy: a case report

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Journal of Geriatric Cardiology 2020年第3期17卷 164-168页

作者： Marcus VH Carvalho priscila C Kroll Vinicius n Carvalho department of Cardiothoracic Medicine JundiaíMedical SchoolJundiaíSPBrazil Transplant Hospital E J Zerbini Sao PauloSPBrazil Municipal Hospital Mario Gatti CampinasSao PauloSPBrazil

Left bundle branch block(LBBB)causes a delay in left ventricular contraction with an unsynchronized ventricular *** is an independent determinant of morbi-mortality mainly when associated with cardiomyopathy and left ventricular dysfunction.[1] LBBB due to non-ischemic cardiomyopathy is considered *** irreversibility occurs because LBBB and cardiomyopathy act in a synergic manner in order to maintain both ***,there are a few reports in the literature showing that some patients have had an improvement in cardiac function with normalization of QRs and have experienced a reverse remodelling with pharmacological therapy only.[2–4]

关键词： desynchronization Heart failure Left bundle branch block Reverse remodelling

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The Use of secondary Grape Biomass in Beef Cattle nutrition on Carcass Characteristics, Quality and shelf Life of Meat

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Food and nutrition sciences 2024年第6期15卷 447-469页

作者： Vitor L. Molosse Guilherme L. deolindo Rafael V. P. Lago Bruna Klein Claiton A. Zotti Marcelo Vedovato Marcylene V. da silveira priscila M. Copetti Maria R. C. schetinger Juscivete F. Favero Eliana L. Fiorentin Aleksandro s. da silva Universidade do Estado de santa Catarina (UdesC) Chapec Brazil department of Animal science UDESC Chapec Brazil department of Animal science Universidade do Oeste de Santa Catarina Xanxere Brazil dean Lee Research and Extension Center Louisiana State University Alexandria LA USA department of Biochemistry Universidade Federal de Santa Maria Santa Maria Brazil department of Medicina Veterinria UNOCHAPEC Chapec Brasil

We determined whether the inclusion of 100 g/kg dry matter of grape pomace silage (GPs) and grape pomace bran (GPB) as substitutes for other traditional fiber sources in the diet of steers (Charolais x nellore) would improve carcass characteristics, meat quality and composition, and shelf life. Twenty-four animals (248 ± 19.32 kg of initial body weight) were fed a high concentrate diet for 121 days. Carcass characteristics were measured, and the longissimus dorsi muscle was analyzed for fatty acid (FA) profile and composition. The meat was sliced and stored in air-permeable packages for 10 days. On each sampling day (d 1, 3, 7, and 10), oxidative stability, bacterial load, lipid and protein oxidation, and staining were analyzed. The experimental diets influenced the pH of cold carcasses only. The GPs group had a higher pH than the control. The GPs and GPB groups showed improved oxidant status (i.e., lower lipid peroxidation and concentrations of reactive oxygen species were in the meat of both groups than in control). On the first day of storage, the antioxidant enzyme glutathione s-transferase activity was more significant in the meat of the GPs and GPB groups than in the control. The bacterial loads in the meat were attenuated by GPs inclusion;there were lower total coliform counts and a trend toward lower counts for enterobacteria in the control group. The diets altered the FA profile of the meat;i.e., the GPB diet allowed for a more significant amount of the n-6 omegas in the meat, while the GPs diet showed a tendency for a more significant amount of n-6 and 9 omegas. Both diets (GPs and GPB) increased the amounts of long-chain FAs. The GPs diet decreased saturated FA levels. We conclude that the dietary treatments GPs and GPB are a promising alternative to maintain meat quality standards throughout in real-world retail conditions. These treatments gave rise to an improvement in the nutritional value of the meat due to the more significant amounts of FAs that im

关键词： Animal nutrition Antioxidant Biomass Grape Meat Quality Microbiology Residue

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