The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen

作     者：Bruno G.N.Andrade Flavia A.Bressani Rafael R.C.Cuadrat Polyana C.Tizioto Priscila S.N.de Oliveira Gerson B.Mourão Luiz L.Coutinho James M.Reecy James E.Koltes Paul Walsh Alexandre Berndt Julio C.P.Palhares Luciana C.A.Regitano Bruno G.N.Andrade;Flavia A.Bressani;Rafael R.C.Cuadrat;Polyana C.Tizioto;Priscila S.N.de Oliveira;Gerson B.Mour(a)o;Luiz L.Coutinho;James M.Reecy;James E.Koltes;Paul Walsh;Alexandre Berndt;Julio C.P.Palhares;Luciana C.A.Regitano

作者机构：Embrapa Southeast LivestockSão CarlosBrazil Department of Molecular EpidemiologyGerman Institute of Human Nutrition Potsdam-Rehbrücke(DIfE)NuthetalGermany NGS Genomic SolutionsPiracicabaBrazil Department of Animal ScienceUniversity of São Paulo/ESALQPiracicabaBrazil Department of Animal ScienceIowa State UniversityAmesIAUSA NSilico Life ScienceDublinIreland 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2020年第11卷第2期

页      面：577-586页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：This study was conducted with funding from EMBRAPA,São Paulo Research Foundation scholarship to BGNA(grant number:2017/12642–8) the National Council for Scientific and Technological Development(CNPq,grant number:428153/2018)and fellowships to LCAR and LLC 

主　　题：Archaea Bacteria Bos indicus Metabarcoding Methanobrevibacter Microbiome Microbiota 

摘      要：Background:The success of different species of ruminants in the colonization of a diverse range of environments is due to their ability to digest and absorb nutrients from cellulose,a complex polysaccharide found in leaves and *** rely on a complex and diverse microbial community,or microbiota,in a unique compartment known as the rumen to break down this *** in microbial populations of the rumen can affect the host’s development,health,and ***,accessing the rumen is stressful for the ***,the development and use of alternative sampling methods are needed if this technique is to be routinely used in cattle *** this end,we tested if the fecal microbiome could be used as a proxy for the rumen microbiome due to its *** investigated the taxonomic composition,diversity and inter-relations of two different GIT compartments,rumen and feces,of 26 Nelore(Bos indicus)bulls,using Next Generation Sequencing(NGS)metabarcoding of bacteria,archaea and ciliate ***:We identified 4265 Amplicon Sequence Variants(ASVs)from bacteria,571 from archaea,and 107 from protozoa,of which 143(96 bacteria and 47 archaea)were found common between both *** most prominent bacterial phyla identified were Bacteroidetes(41.48%)and Firmicutes(56.86%)in the ruminal and fecal microbiomes,respectively,with Prevotella and Ruminococcaceae UCG-005 the most relatively abundant genera identified in each *** most abundant archaeal phylum identified was Euryarchaeota,of which Methanobrevibacter gottschalkii,a methanogen,was the prevalent archaeal species identified in both *** were found exclusively identified in the rumen with Bozasella/Triplumaria being the most frequent genus ***-occurrence among ruminal and fecal ASVs reinforces the relationship of microorganisms within a biological ***,the co-occurrence of shared archaeal ASVs between microbiomes indicat