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The structure of microbial populations in nelore GIT reveals inter-dependency of methanogens in feces and rumen

Journal of Animal science and Biotechnology 2020年第2期11卷 577-586页

作者： Bruno G.n.Andrade Flavia A.Bressani Rafael R.C.Cuadrat Polyana C.Tizioto priscila s.n.de oliveira Gerson B.Mourão Luiz L.Coutinho James M.Reecy James E.Koltes Paul Walsh Alexandre Berndt Julio C.P.Palhares Luciana C.A.Regitano Embrapa southeast Livestock São CarlosBrazil department of Molecular Epidemiology German Institute of Human Nutrition Potsdam-Rehbrücke(DIfE)NuthetalGermany nGs Genomic solutions PiracicabaBrazil department of Animal science University of São Paulo/ESALQPiracicabaBrazil department of Animal science Iowa State UniversityAmesIAUSA nsilico Life science DublinIreland

Background:The success of different species of ruminants in the colonization of a diverse range of environments is due to their ability to digest and absorb nutrients from cellulose,a complex polysaccharide found in leaves and *** rely on a complex and diverse microbial community,or microbiota,in a unique compartment known as the rumen to break down this *** in microbial populations of the rumen can affect the host’s development,health,and ***,accessing the rumen is stressful for the ***,the development and use of alternative sampling methods are needed if this technique is to be routinely used in cattle *** this end,we tested if the fecal microbiome could be used as a proxy for the rumen microbiome due to its *** investigated the taxonomic composition,diversity and inter-relations of two different GIT compartments,rumen and feces,of 26 nelore(Bos indicus)bulls,using next Generation sequencing(nGs)metabarcoding of bacteria,archaea and ciliate ***:We identified 4265 Amplicon sequence Variants(AsVs)from bacteria,571 from archaea,and 107 from protozoa,of which 143(96 bacteria and 47 archaea)were found common between both *** most prominent bacterial phyla identified were Bacteroidetes(41.48%)and Firmicutes(56.86%)in the ruminal and fecal microbiomes,respectively,with Prevotella and Ruminococcaceae UCG-005 the most relatively abundant genera identified in each *** most abundant archaeal phylum identified was Euryarchaeota,of which Methanobrevibacter gottschalkii,a methanogen,was the prevalent archaeal species identified in both *** were found exclusively identified in the rumen with Bozasella/Triplumaria being the most frequent genus ***-occurrence among ruminal and fecal AsVs reinforces the relationship of microorganisms within a biological ***,the co-occurrence of shared archaeal AsVs between microbiomes indicat

关键词： Archaea Bacteria Bos indicus Metabarcoding Methanobrevibacter Microbiome Microbiota

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Effect of silymarin on biochemical indicators in patients with liver disease: systematic review with meta-analysis

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World Journal of Gastroenterology 2017年第27期23卷 5004-5017页

作者： Camila Ribeiro de Avelar Emile Miranda Pereira priscila Ribas de Farias Costa Rosangela Passos de Jesus Lucivalda Pereira Magalhaes de oliveira Postgraduation Program Federal University of Bahia departament of science nutrition Federal University of Bahia

To evaluate the effect of silymarin on the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AsT) and gamma glutamyl transpeptidase (γGT) in patients with liver diseases. METHODsA systematic review with meta-analysis of ramdomized and controlled clinical trials was performed, evaluating the effects of sylimarin in patients with hepatic diseases, published by January 31, 2016. Clinical trials were sought on the basis of The Cochrane Central Register of Controlled Trials in the Cochrane Library, PubMed/Medline, scopus, Web of science, Lilacs and Clinical Trials. The trials with adult and elderly patients of both sexes, with Liver Diseases who took oral silymarin supplementation, as extract or isolated, as well as silymarin combined with other nutrients, were included. The trials should provide information about the intervention, such as dosages and detailing of the product used, besides the mean and standard deviation of serum levels of ALT, AsT and γGT of the baseline and at the end of the intervention. REsULTsAn amount of 10904 publications were identified. From those, only 17 were included in the systematic review and 6 in the meta-analysis, according to the used selection criteria. In this meta-analysis, the results indicated a reduction of 0.26 IU/mL (95%CI: -0.46-0.07, P = 0.007) at the level of ALT and 0.53 IU/mL (95%CI: -0.74-0.32, P = 0.000) at the serum levels of AsT after using the silymarin, both, statistically significant, but with no clinical relevance. There was no significant change in the γGT levels. subgroup analyzes were also performed for the biochemical markers in relation to the type of intervention, whether silymarin isolated or associated with other nutrients and the time of intervention (whether ≥ 6 mo or significant differences were not found. The evaluated studies presented a high degree of heterogeneity and low methodological quality in the carried out analysis. COnCLUsIOnsilymarin minimally reduced, but w

关键词： systematic review Liver diseases Milk thistle silymarin Meta-analysis

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Buzz-pollination in neotropical bees:genus-dependent frequencies and lack of optimal frequency for pollen release

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Insect science 2020年第1期27卷 133-142页

作者： Conrado Augusto Rosi-denadai priscila Cassia souza Araujo Lucio Antonio de oliveira Campos Lirio Cosme Jr. Raul narciso Carvalho Guedes departamento de Entomologia Universidade Federal de VigosaVigosaMinas GeraisBrazil departamento de Biologia Geral Universidade Federal de VigosaVigosaMinas GeraisBrazil

Over 50 genera of bees release pollen from flower anthers using thoracic vibrations,a phenomenon known as *** efficiency of this process is directly affected by the mechanical properties of the buzzes,namely the duration,amplitude,and ***,although the effects of the former two properties are well described,the role of buzz frequency on pollen release remains ***,nearly all of the existing studies describing vibrational properties of natural buzz-pollination are limited to bumblebees(Bombus)and carpenter bees(Xvlocopa)constraining our current understanding of this behavior and its ***,we attempted to minimize this shortcoming by testing whether flower anthers exhibit optimal frequency for pollen release and whether bees tune their buzzes to match these(optimal)*** true,certain frequencies will trigger more pollen release and lighter bees will reach buzz frequencies closer to this optimum to compensate their smaller buzz *** strategies were used to test these hypotheses:(i)the use of(artificial)vibrational playbacks in a broad range of buzz frequencies and amplitudes to assess pollen release by tomato plants(solarium Ivcopersicum L.)and(ii)the recording of natural buzzes of neotropical bees visiting tomato plants during *** playback experiment indicates that although buzz frequency does affect pollen release,no optimal frequency exists for *** addition,the recorded results of natural buzz-pollination reveal that buzz frequencies vary with bee genera and are not correlated with body ***,neither bees nor plants are tuned to optimal pollen release *** frequency of buzz-pollination is a likely consequence of the insect flight machinery adapted to reach higher accelerations,while flower plant response to buzz-pollination is the likely result of its pollen granular properties.

关键词： buzz amplitude buzz frequency floral sonication pollination stingless bees vibration

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Black,pinto and white beans lower hepatic lipids in hamsters fed high fat diets by excretion of bile acids

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Food Production, Processing and nutrition 2020年第1期2卷 249-257页

作者： priscila L.s.Alves Jose de J Berrios James Pan Wallace H.Yokoyama Agricultural Research service United States Department of AgricultureHealthy Processed Foods ResearchWestern Regional Research Center800 Buchanan St.AlbanyCA 94710USA

Hypolipidemic and anti-obesity properties of extruded black,pinto and white beans were examined in male syrian hamsters fed high fat diets for 3 *** efficiency and effectiveness of extruder processing to eliminate heatlabile antinutrients were also *** fed a high fat diet containing 40%extruded black beans gained the same amount of weight as animals on a low-fat chow diet(based on AIn-93G).Total plasma cholesterol of the hamsters fed bean flour-based diets tended to be lower,ranging between 298 and 356 mg/dL,compared to 365 mg/dL for the control group and total and free liver cholesterol was about 50 and 33%lower,respectively,in the livers(freeze-dried)of hamsters fed the black turtle bean(BB)and pinto bean(PB)*** nitrogen content of the feces from hamsters fed the PB and white bean(WB)diets were 2.6x higher than the control and the excretion of deoxycholic acid(DCA)and lithocholic acid(LCA)was higher in all legume fed hamsters The results show that extrusion efficiently and economically reduces anti-nutritive factors that inhibit the digestion and absorption of proteins and carbohydrates.

关键词： Beans Extrusion Anti-obesity Bile acid Mice

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Genomic regions and pathways associated with gastrointestinal parasites resistance in santa Inês breed adapted to tropical climate

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Journal of Animal science and Biotechnology 2018年第1期9卷 35-50页

作者： Mariana Piatto Berton Rafael Medeiros de oliveira silva Elisa Peripolli nedenia Bonvino stafuzza Jesús Fernández Martin Maria saura álvarez Beatriz Villanueva Gavina Miguel Angel Toro Georgget Banchero priscila silva oliveira Joanir Pereira Eler Fernando Baldi José Bento sterman Ferraz departamento de Zootecnia Faculdade de Ciências Agrárias e VeterináriasUniversidade Estadual PaulistaVia de acesso Prof.Paulo Donato Castellane sno Jaboticabal SP CEP 14884 900 Brazil Instituto nacional de Investigación y Tecnología Agraria y Alimentaria InIA Crta.de la Coruna km75 28040 Madrid Spain departamento de Producción Agraria School of AgriculturalFood and Byosystems EngineeringUniversisdad Politécnica de MadridCampus Ciudad Universitaria Avda.Complutense 3-Avda.Puerta Hierro 28040 Madrid Spain Instituto nacional de Investigación Agropecuária(InIA) Ruta 50 Kin. 12 Colonia Uruguay Faculdade de Zootecnia e Engenharia de Alimentos Nucleo de Apoio à Pesquisa em Melhoramento AnimalBiotecnologia e TransgeniaUniversidade de Sao Paulo Rua Duque de Caxias Norte 225 Pirassununga SP CEP 13635 900Brazil

Background： The aim of this study was to estimate variance components and to identify genomic regions and pathways associated with resistance to gastrointestinal parasites, particularly Haemonchus contortus, in a breed of sheep adapted to tropical climate. Phenotypes evaluations were performed to verify resistance to gastrointestinal parasites, and were divided into two categories： i） farm phenotypes, assessing body condition score（BCs）, degree of anemia assessed by the famacha chart（FAM）, fur score（Fs） and feces consistency（FC）; and ii） lab phenotypes,comprising blood analyses for hematocrit（HCT）, white blood cell count（WBC）, red blood cell count（RBC）,hemoglobin（HGB）, platelets（PLT） and transformed（log10） egg per gram of feces（EPG_（log））. A total of 576 animals were genotyped with the Ovine snP12k Bead Chip（Illumina, Inc.）, that contains 12,785 bialleleic snP markers. The variance components were estimated using a single trait model by single step genomic BLUP ***： The overall linkage disequilibrium（LD） mean between pairs of markers measured by r2 was 0.23. The overall LD mean between markers considering windows up to 10 Mb was 0.07. The mean LD between adjacent snPs across autosomes ranged from 0.02 to 0.10. Heritability estimates were low for EPG_（log）（0.11）, moderate for RBC（0.18）, PLT（0.17） HCT（0.20）, HGB（0.16） and WBC（0.22）, and high for FAM（0.35）. A total of 22, 21, 23, 20, 26, 25 and 23 windows for EPG_（log）for FAM, WBC, RBC, PLT, HCT and HGB traits were identified, respectively. Among the associated windows, 10 were shown to be common to HCT and HGB traits on OAR1, OAR2, OAR3, OAR5, OAR8 and ***： The traits indicating gastrointestinal parasites resistance presented an adequate genetic variability to respond to selection in santa Inês breed, and it is expected a higher genetic gain for FAM trait when compared to the others. The level of LD estimated for markers separated by less than 1 Mb indicated that the Ovine snP12k Bead Chip m

关键词： Gwas Linkage disequilibrium arasites resistance santa Inês breed

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delayed spontaneous reversibility of left bundle branch block in non-ischemic cardiomyopathy: a case report

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Journal of Geriatric Cardiology 2020年第3期17卷 164-168页

作者： Marcus VH Carvalho priscila C Kroll Vinicius n Carvalho department of Cardiothoracic Medicine JundiaíMedical SchoolJundiaíSPBrazil Transplant Hospital E J Zerbini Sao PauloSPBrazil Municipal Hospital Mario Gatti CampinasSao PauloSPBrazil

Left bundle branch block(LBBB)causes a delay in left ventricular contraction with an unsynchronized ventricular *** is an independent determinant of morbi-mortality mainly when associated with cardiomyopathy and left ventricular dysfunction.[1] LBBB due to non-ischemic cardiomyopathy is considered *** irreversibility occurs because LBBB and cardiomyopathy act in a synergic manner in order to maintain both ***,there are a few reports in the literature showing that some patients have had an improvement in cardiac function with normalization of QRs and have experienced a reverse remodelling with pharmacological therapy only.[2–4]

关键词： desynchronization Heart failure Left bundle branch block Reverse remodelling

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Transmission of cryptococcosis by liver transplantation:A case report and review of literature

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World Journal of Hepatology 2020年第5期12卷 253-261页

作者： Gustavo de sousa Arantes Ferreira Andre Luis Conde Watanabe natalia de Carvalho Trevizoli Fernando Marcus Felippe Jorge Carolina de Fatima Couto priscila Brizolla de Campos Gabriel oliveira nunes Caja department of Liver Transplantation Instituto de Cardiologia do Distrito FederalBrasilia 70673900Brazil

BACKGROUnD Cryptococcosis is a fungal infection caused by the yeast-like encapsulated basidiomycetous fungus of the Cryptococcus neoformans(***)species *** fungi are ubiquitous in soil and bird droppings,and infection by them is an important global health concern,particularly in immunosuppressed patients,such as organ transplant recipients and those infected by the human immunodeficiency *** fungus usually enters the body through the respiratory tract,but extremely rare cases of infection acquired by transplantation of solid organs have been *** sUMMARY We report a case of disseminated cryptococcosis in a liver transplant recipient,diagnosed 2 wk after the *** patient initially presented with fever,hyponatremia and elevated transaminase levels,manifesting intense headache after a few *** cultures were positive for *** biopsy showed numerous fungal elements surrounded by gelatinous matrix and sparse granulomatous *** resonance imaging of the brain showed multiple small lesions with low signal in T2,peripheric enhancement and edematous halo,diffuse through the parenchyma but more concentrated in the subcortical *** with amphotericin B for 3 wk,followed by maintenance therapy with fluconazole,led to complete resolution of the *** recipients of both kidneys from the same donor also developed disseminated cryptococcosis,confirming the transplant as the source of *** organ donor lived in a rural area,surrounded by tropical rainforest,and had negative blood cultures prior to organ *** This case highlights the risk of transmission of fungal diseases,specifically of ***,through liver graft during liver transplantation.

关键词： Cryptococcosis Liver transplantation Cryptococcus neoformans Case report Immunosuppression Fungal infection

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Active packaging for lipid foods and development challenges for marketing

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Food Bioscience 2022年第1期45卷 20-33页

作者： Erica da Costa Moncao Cristiani Viegas Brandao Grisi Janaína de Moura Fernandes priscila santos souza Antonia Lucia de souza Programa de Pos-Graduacao Em Ciencia e Tecnologia de Alimentos Universidade Federal da ParaíbaJoao PessoaPB58051Brazil Programa de Pos-Graduacao Em Tecnologia Agroalimentar Universidade Federal da ParaíbaBananeiras-PB58220-000Brazil Programa de Pos-Graduacao Em Química Universidade Federal da ParaíbaJoao PessoaPB58051Brazil

Consumer demand for natural products with low concentration of additives and controlled environmental impact has been a major challenge for the lipid food packaging *** of this fact,active packaging such as oxygen absorbers and antioxidant releasers are trends in the *** this work,research developed on oxygen absorbing packaging and antioxidant-releasing packaging will be *** work will also reveal the challenges to *** that slows down lipid oxidation in food presents satisfactory results and greater efficacy than vacuum *** challenges for the development of active packaging that consist of biopolymers and natural additives are related to the physical and mechanical properties of the *** research should develop biodegradable active packaging that are resistant to industrial ***,there are plenty of natural resources not yet studied and that may be applicable for the development of active oxygen-absorbing and antioxidant active packaging for food.

关键词： Oxygen absorbers Antioxidants Food packaging Lipids Oxidation

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Bioengineering breakthroughs:The impact of stem cell models on advanced therapy medicinal product development

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World Journal of stem Cells 2024年第10期16卷 860-872页

作者： José Mauro Granjeiro priscila Grion de Miranda Borchio Icaro Paschoal Brito Ribeiro Katiucia Batista silva Paiva Division of Biological Metrology The National Institute of MetrologyQualityand TechnologyDuque de Caxias 25250020Rio de JaneiroBrazil Laboratory of Regenerative Medicine Arthur SáEarp Neto UniversityPetropolis 25680120Rio de JaneiroBrazil Laboratory of Extracellular Matrix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508000São PauloBrazil

The burgeoning field of bioengineering has witnessed significant strides due to the advent of stem cell models,particularly in their application in advanced therapy medicinal products(ATMPs).In this review,we examine the multifaceted impact of these developments,emphasizing the potential of stem cell models to enhance the sophistication of ATMPs and to offer alternatives to animal *** cell-derived tissues are particularly promising because they can reshape the preclinical landscape by providing more physiologically relevant and ethically sound platforms for drug screening and disease *** also discuss the critical challenges of reproducibility and accuracy in measurements to ensure the integrity and utility of stem cell models in research and ***,this review highlights the imperative of stem cell models to align with regulatory standards,ensuring using stem cells in ATMPs translates into safe and effective clinical *** regulatory approval serving as a gateway to clinical adoption,the collaborative efforts between scientists and regulators are vital for the progression of stem cell applications from bench to *** advocate for a balanced approach that nurtures innovation within the framework of rigorous validation and regulatory compliance,ensuring that stem cell-base solutions are maximized to promote public trust and patient health in ATMPs.

关键词： stem cells Advanced therapy medicinal products Tissue-engineered products Health Three-dimensional cell culture

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The Control of Lactobacillus *** Extracellular Compound Produced by Pseudomonas aeruginosa in the Fermentation Process of Fuel Ethanol Industry in Brazil

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Journal of sustainable Bioenergy systems 2013年第3期3卷 194-201页

作者： Cintia Greice Matsuoca Gois Lucilene Lopes-santos Jamile priscila de oliveira Beranger Admilton Goncalves de oliveira Flavia Regina spago Galdino Andrade departamento de Microbiologia Laboratorio de Ecologia MicrobianaUniversidade Estadual de LondrinaLondrinaBrazil

This work evaluated the effect of secondary bacterial metabolites produced by Pseudomonas sp LV strain in control of Lactobacillus sp. population in the microcosm of the vat during ethanol fermentation. The fraction F4 produced by Pseudomonas aeruginosa was extracted with dichloromethane and fractionating by vacuum liquid chromatography obtained in a methanol phase. The evaluation of antibiotic activity of F4 fraction mixed or not with sulphuric acid and Kamoram?. The antibiotic activity of F4 fraction was determined as well as the fermentation efficiency. Also was determined yeast cell viability, budding formation, the viability of budding cells, and number of populations of saccharomyces cerevisiae and Lactobacillus sp. The results showed that the F4 fraction had high selective antibiotic activity against Lactobacillus sp. but not for s. cerevisae, and no inhibitory effect was observed in the fermentation process by yeast. Also F4 fraction decreased flocculation and foam formation. The F4 has an antibiotic activity against Lactobacillus sp. and should be used as an alternative to control bacteria contamination and foam and flocculation formation in the fuel ethanol fermentation process. The F4 fraction could reduce the use of antibiotics in the control of Lactobacillus sp. population during the fuel ethanol production.

关键词： Fuel Ethanol Fermentation Contamination Lactobacillus Yeast Pseudomonas

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