检索结果-南通市图书馆

生物相容性量子点的表征及其在细胞标记中的应用

在线全文

学校读者我要写书评

暂无评论

光谱学与光谱分析 2010年第5期30卷 1290-1294页

作者：刘夏陈丹妮屈军乐杨坚泰罗永祥 Roy Indrajit 王晓梅林晓潭钟磊 prasad n paras 许改霞牛憨笨深圳大学光电工程学院光电子器件与系统(教育部广东省)重点实验室广东深圳518060 美国纽约州立大学布法罗分校激光光子与生物光子学研究所布法罗美国142603000 深圳大学医学院深圳市生物医学工程重点实验室广东深圳518060

利用无机低温水相合成法制备表面包覆L-半胱氨酸的CdTe/ZnTe核壳型量子点,测量不同溶液以及激发功率激光作用下该量子点的光谱特性。结果表明,该量子点吸收谱和荧光光谱峰值位置不随pH值变化,而荧光光强随pH值升高呈近似线性上升趋势;... 详细信息

利用无机低温水相合成法制备表面包覆L-半胱氨酸的CdTe/ZnTe核壳型量子点,测量不同溶液以及激发功率激光作用下该量子点的光谱特性。结果表明,该量子点吸收谱和荧光光谱峰值位置不随pH值变化,而荧光光强随pH值升高呈近似线性上升趋势;不同缓冲液对该量子点荧光光强无影响,但随孵育时间延长,荧光强度略有下降;在强激光照射下QDs会被快速光漂白,选择适当的激光功率(<100μW),可降低漂白速率,实现长时间稳定测量。因此,该量子点具有较好的生物稳定性和光稳定性,将其与血铁蛋白相连形成靶向共轭纳米粒,可成功用于HeLa细胞标记。细胞内量子点光漂白实验表明,细胞微环境会影响量子点的光稳定性,加速量子点的光漂白。

关键词： CdTe/ZnTe QDs 细胞标记

Fluorescence lifetime imaging for studying DnA compaction and gene activities

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2021年第12期10卷 2349-2359页

作者： Svitlana M.Levchenko Artem Pliss Xiao Peng paras n.prasad Junle Qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhenGuangdong 518060China Department of Cell Biophysics Faculty of BiochemistryBiophysics and BiotechnologyJagiellonian University30-387 KrakowPoland Institute for Lasers Photonics and BiophotonicsUniversity at BuffaloState University of New YorkBuffaloNY 14260-3000USA

Optical imaging is a most useful and widespread technique for the investigation of the structure and function of the cellular ***,an analysis of immensely convoluted and irregularly compacted DnA polymer is highly challenging even by modern super-resolution microscopy *** we propose fluorescence lifetime imaging(FLIM)for the advancement of studies of genomic structure including DnA compaction,replication as well as monitoring of gene *** proposed FLIM assay employs two independent mechanisms for DnA compaction *** mechanism relies on the inverse quadratic relation between the fluorescence lifetimes of fluorescence probes incorporated into DnA and their local refractive index,variable due to DnA compaction *** mechanism is based on the Förster resonance energy transfer(FRET)process between the donor and the acceptor fluorophores,both incorporated into *** these proposed mechanisms were validated in cultured *** obtained data unravel a significant difference in compaction of the gene-rich and gene-poor pools of genomic *** show that the gene-rich DnA is loosely compacted compared to the dense DnA domains devoid of active genes.

关键词： expression. lifetime donor

Initial Experience of nIR-II Fluorescence Imaging-Guided Surgery in Foot and Ankle Surgery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering 2024年第9期40卷 19-27页

作者： Xiaoxiao Fan Jie Yang Huwei ni Qiming Xia Xiaolong Liu Tianxiang Wu Lin Li paras n.prasad Chao Liu Hui Lin Jun Qian Department of General Surgery Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016China Centre for Optical and Electromagnetic Research&International Research Center for Advanced Photonics College of Optical Science and EngineeringZhejiang UniversityHangzhou 310058China Department of Orthopedics Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016China Institute for Lasers Photonicsand BiophotonicsDepartment of ChemistryState University of New YorkBuffaloNY 14260-3000USA Zhejiang Engineering Research Center of Cognitive Healthcare Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016China College of Biomedical Engineering and Instrument Science Zhejiang UniversityHangzhou 310027China

Optical imaging in the second near-infrared(nIR-II;900-1880 nm)window is currently a popular research topic in the field of biomedical *** study aimed to explore the application value of nIR-II fluorescence imaging in foot and ankle surgeries.A lab-established nIR-II fluorescence surgical navigation system was developed and used to navigate foot and ankle surgeries which enabled obtaining more high-spatial-frequency information and a higher signal-to-background ratio(SBR)in nIR-II fluorescence images compared to nIR-I fluorescence images;our result demonstrates that nIR-II imaging could provide higher-contrast and larger-depth images to *** types of clinical application scenarios(diabetic foot,calcaneal fracture,and lower extremity trauma)were included in this *** the nIR-II fluorescence imaging technique,we observed the ischemic region in the diabetic foot before morphological alterations,accurately determined the boundary of the ischemic region in the surgical incision,and fully assessed the blood supply condition of the ***-II fluorescence imaging can help surgeons precisely judge surgical margins,detect ischemic lesions early,and dynamically trace the perfusion *** believe that portable and reliable nIR-II fluorescence imaging equipment and additional functional fluorescent probes can play crucial roles in precision surgery.

关键词： Second near-infrared fluorescence imaging Foot and ankle surgery Indocyanine green Imaging-guided surgery

Water‑Dispersible CsPbBr_(3) Perovskite nanocrystals with Ultra‑Stability and its Application in Electrochemical CO_(2) Reduction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

nano-Micro Letters 2021年第11期13卷 190-202页

作者： Keqiang Chen Kun Qi Tong Zhou Tingqiang Yang Yupeng Zhang Zhinan Guo Chang‑Keun Lim Jiayong Zhang Igor Zutic Han Zhang paras n.prasad Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of Microscale OptoelectronicsShenzhen UniversityShenzhen 518060People’s Republic of China Institute for Lasers Photonicsand BiophotonicsDepartment of ChemistryUniversity at BuffaloState University of New YorkBuffaloNY 14260USA Department of Physics University at BuffaloState University of New YorkBuffaloNY 14260USA Department of Chemical and Materials Engineering School of EngineeringNazarbayev UniversityNur‑Sultan City 010000Kazakhstan Jiangsu Key Laboratory of Micro and nano Heat Fluid Flow Technology and Energy Application School of Physical Science and TechnologySuzhou University of Science and TechnologySuzhouJiangsu 215009People’s Republic of China

Thanks to the excellent optoelectronic properties,lead halide perovskites(LHPs)have been widely employed in highperformance optoelectronic devices such as solar cells and lightemitting ***,overcoming their poor stability against water has been one of the biggest challenges for most ***,we report a novel hot-injection method in a Pb-poor environment combined with a well-designed purification process to synthesize water-dispersible CsPbBr_(3) nanocrystals(nCs).The as-prepared nCs sustain their superior photoluminescence(91%quantum yield in water)for more than 200 days in an aqueous environment,which is attributed to a passivation effect induced by excess CsBr *** to the ultra-stability of these LHP nCs,for the first time,we report a new application of LHP nCs,in which they are applied to electrocatalysis of CO_(2) reduction ***,they show significant electrocatalytic activity(faradaic yield:32%for CH4,40%for CO)and operation stability(>350 h).

关键词： CsPbBr_(3)nanocrystals Water-dispersible Ultra-stability Electrochemical CO_(2)reduction

Black phosphorus-based photothermal therapy with aCD47-mediated immune checkpoint blockade for enhanced cancer immunotherapy

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2020年第1期9卷 479-493页

作者： Zhongjian Xie Minhua Peng Ruitao Lu Xiangying Meng Weiyuan Liang Zhongjun Li Meng Qiu Bin Zhang Guohui nie ni Xie Han Zhang paras n.prasad Shenzhen International Institute for Biomedical Research Shenzhen 518116 GuangdongPR China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhen 518055 GuangdongPR China School of Basic Medical Sciences Guangzhou Medical UniversityGuangzhou 511436 GuangdongPR China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of Microscale Optoelectronicsand Otolaryngology Department and Biobank of the First Affiliated HospitalShenzhen Second People’s HospitalHealth Science CenterShenzhen UniversityShenzhen 518060PR China Key Laboratory of Marine Chemistry Theory and Technology(Ocean University of China) Ministry of EducationQingdao 266100PR China Department of Chemistry Institute for LasersPhotonicsand BiophotonicsUniversity at BuffaloStateUniversity of New YorkBuffaloNYUSA

Here,we describe a combination strategy of black phosphorus(BP)-based photothermal therapy together with anti-CD47 antibody(aCD47)-based immunotherapy to synergistically enhance cancer *** resistance to immune checkpoint blockades in most cancers due to immune escape from host surveillance,along with the initiation of metastasis through immunosuppressive cells in the tumour microenvironment,remains a significant challenge for cancer ***47,an agent for CD47/SIRPαaxis blockade,induces modest phagocytic activity and a low response rate for monotherapy,resulting in failures in clinical *** showed that BP-mediated ablation of tumours through photothermal effects could serve as an effective strategy for specific immunological stimulation,improving the inherently poor immunogenicity of tumours,which is particularly useful for enhancing cancer *** in combination with aCD47 blockade activates both innate and adaptive immunities and promotes local and systemic anticancer immune responses,thus offering a synergistically enhanced effect in suppression of tumour progression and in inducing abscopal effects for inhibition of metastatic *** combination strategy provides a promising platform in which photothermal agents could help to enhance the therapeutic efficacy of immunotherapy.

关键词： immunotherapy cancer mediated

Uptake of transferrin-conjugated quantum dots in single living cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Optics Letters 2010年第10期8卷 940-943页

作者：陈丹妮许改霞 Bahi Ahmed Ali Ken-Tye Yong 周翠红王晓梅屈军乐 paras n.prasad 牛憨笨 College of Optoelectronic Science and Engineering Huazhong University of Science and Technology Institute of Optoelectronics Key Lab of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province Shenzhen Key Lab of Biomedicine Engineering Shenzhen University College of Medicine Shenzhen University Mubarak City for Scientific Research and Technology Applications School of Electrical and Electronic Engineering Nanyang Technological University Institute for Laser Photonics and Biophotonics State University of New York at Buffalo

We study the uptake and distribution of transferrin （Tf）-conjugated CdSe/CdS/ZnS quantum dots （QDs） in single living HeLa cells with both fluorescence confocal microscopy and three-dimensional （3D） reconstruction technique. By increasing the co-incubation time or the dosage of QDs-Tf, we find that the uptake of QDs-Tf bioconjugates in the cells increases correspondingly, but with different uptake rates. Additionally, the distribution of QDs-Tf, in single live HeLa cells is time dependent. To our knowledge, this is the first study on quantitatively analyzing the uptake and distribution of bioconjugated QDs in single living cells. Such QDs nanoplatform can be further modified for developing biomedical evaluation tool in cancer diagnosis and targeted drug delivery.

关键词： Confocal microscopy Drug delivery Three dimensional

Hot-band absorption of indocyanine green for advanced anti-stokes fluorescence bioimaging

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2021年第10期10卷 1877-1888页

作者： Jing Zhou Xiaoxiao Fan Di Wu Jie Liu Yuhuang Zhang Zikang Ye Dingwei Xue Mubin He Liang Zhu Zhe Feng Andrey n.Kuzmin Wen Liu paras n.prasad Jun Qian State Key Laboratory of Modern Optical Instrumentations Centre for Optical and Electromagnetic ResearchCollege of Optical Science and EngineeringInternational Research Center for Advanced PhotonicsZhejiang UniversityHangzhou 310058China Sir Run-Run Shaw Hospital School of MedicineZhejiang UniversityHangzhou 310016China Key Laboratory of Flexible Electronics(KLOFE)Institute of Advanced Materials(IAM) Nanjing Tech University(Nanjing Tech)Nanjing 211800China Department of Chemistry Zhejiang UniversityHangzhou 310058China Interdisciplinary Institute of neuroscience and Technology(ZIInT) College of Biomedical Engineering and Instrument ScienceZhejiang UniversityHangzhou 310027China Institute for Lasers Photonicsand BiophotonicsDepartment of ChemistryUniversity at BuffaloState University of New YorkBuffaloNY 14260USA Key Laboratory of Optical Information Detection and Display Technology of Zhejiang Zhejiang Normal UniversityJinhua 321004China

Bright anti-Stokes fluorescence(ASF)in the first near-infrared spectral region(nIR-I,800 nm–900 nm)under the excitation of a 915 nm continuous wave(CW)laser,is observed in Indocyanine Green(ICG),a dye approved by the Food and Drug Administration for clinical *** dependence of fluorescence intensity on excitation light power and temperature,together with fluorescence lifetime measurement,establish this ASF to be originated from absorption from a thermally excited vibrational level(hot-band absorption),as shown in our experiments,which is stronger than the upconversion fluorescence from widely-used rare-earth ion doped *** test the utility of this ASF nIR-I probe for advanced bioimaging,we successively apply it for biothermal sensing,cerebral blood vessel tomography and blood stream ***,in combination with L1057 nanoparticles,which absorb the ASF of ICG and emit beyond 1100 nm,these two probes generate multi-mode images in two fluorescent channels under the excitation of a single 915 nm CW *** channel is used to monitor two overlapping organs,urinary system&blood vessel of a live mouse,while the other shows urinary system *** in intraoperative real-time monitoring,such multi-mode imaging method can be beneficial for visual guiding in anatomy of the urinary system to avoid any accidental injury to the surrounding blood vessels during surgery.

关键词： absorption excitation anatomy

Multi-Objective High-Fidelity Optimization Using nSGA-III and MO-RPSOLC

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Computer Modeling in Engineering & Sciences 2021年第11期129卷 443-464页

作者： n.Ganesh Uvaraja Ragavendran Kanak Kalita paras Jain Xiao-Zhi Gao Department of Computer Science and Engineering Vel Tech Multi Tech Dr.Rangarajan Dr.Sakunthala Engineering CollegeChennai600062India Department of Electronics and Telecommunication Engineering MPSTME SVKM’S Narsee Monjee Institute of Management StudiesShirpur425405India Department of Mechanical Engineering Vel Tech Rangarajan Dr.Sagunthala R&D Institute of Science and TechnologyAvadi600062India School of Computing Science and Engineering VIT Bhopal UniversitySehore466114India School of Computing University of Eastern FinlandKuopio70211Finland

Optimizing the performance of composite structures is a real-world application with significant *** this paper,a high-fidelity finite element method(FEM)is combined with the iterative improvement capability of metaheuristic optimization algorithms to obtain optimized composite *** FEM module comprises of ninenode isoparametric plate bending element in conjunction with the first-order shear deformation theory(FSDT).A recently proposed memetic version of particle swarm optimization called RPSOLC is modified in the current research to carry out multi-objective Pareto *** performance of the MO-RPSOLC is found to be comparable with the *** work successfully highlights the use of FEM-MO-RPSOLC in obtaining highfidelity Pareto solutions considering simultaneous maximization of the fundamental frequency and frequency separation in laminated composites by optimizing the stacking sequence.

关键词： Composites finite element optimization Pareto swarm intelligence

Light-induced tumor theranostics based on chemical-exfoliated borophene

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light(Science & Applications) 2022年第12期11卷 2886-2899页

作者： Zhongjian Xie Yanhong Duo Taojian Fan Yao Zhu Shuai Feng Chuanbo Li Honglian Guo Yanqi Ge Shakeel Ahmed Weichun Huang Huiling Liu Ling Qi Rui Guo Defa Li paras n.prasad Han Zhang Institute of Pediatrics Shenzhen Children’s HospitalShenzhenGuangdongChina Shenzhen Engineering Laboratory of phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationShenzhen Institute of Translational MedicineDepartment of OtolaryngologyShenzhen Second People’s Hospitalthe First Affiliated HospitalInstitute of Microscale OptoelectronicsShenzhen University518060 ShenzhenChina Department of Microbiology Tumor and Cell Biology(MTC)Karolinska InstituteStockholmSweden Shenzhen Medical Ultrasound Engineering Center Department of UltrasonographyShenzhen People’s HospitalSecond Clinical Medical College of Jinan UniversityFirst Clinical Medical College of Southern University of Science and Technology518020 ShenzhenChina Optoelectronics Research Center School of ScienceMinzu University of China100081 BeijingPR China nantong Key Lab of Intelligent and new Energy Materials College of Chemistry and Chemical EngineeringNantong University226019 NantongJiangsuChina Key Laboratory of Biomaterials of Guangdong Higher Education Institutes Guangdong Provincial Engineering and Technological Research Centre for Drug Carrier DevelopmentDepartment of Biomedical EngineeringJinan University510632 GuangzhouChina Department of Core Medical Laboratory the Sixth Affiliated Hospital of Guangzhou Medical UniversityQingyuan People’s HospitalQingyuanGuang Dong ProvinceChina Department of Laboratory Medicine Shenzhen Children’s HospitalShenzhenGuangdongChina Institute for Lasers Photonicsand Biophotonics and Department of ChemistryUniversity at BuffaloState University of New YorkBuffaloNYUSA

Among 2D materials(Xenes)which are at the forefront of research activities,borophene,is an exciting new entry due to its uniquely varied optical,electronic,and chemical properties in many polymorphic forms with widely varying band gaps including the lightest 2D metallic *** this paper,we used a simple selective chemical etching to prepare borophene with a strong near IR light-induced photothermal *** photothermal efficiency is similar to plasmonic Au nanoparticles,with the added benefit of borophene being degradable due to electron deficiency of *** introduce this selective chemical etching process to obtain ultrathin and large borophene nanosheets(thickness of ~4 nm and lateral size up to ~600 nm)from the precursor of AlB_(2).We also report first-time observation of a selective Acid etching behavior showing HCl etching of Al to form a residual B lattice,while HF selectively etches B to yield an Al *** demonstrate that through surface modification with polydopamine(PDA),a biocompatible smart delivery nanoplatform of B@PDA can respond to a tumor environment,exhibiting an enhanced cellular uptake *** demonstrate that borophene can be more suitable for safe photothermal theranostic of thick tumor using deep penetrating near IR light compared to gold nanoparticles which are not degradable,thus posing longterm toxicity *** about 40 kinds of borides,we hope that our work will open door to more discoveries of this top-down selective etching approach for generating borophene structures with rich unexplored thermal,electronic,and optical properties for many other technological applications.

关键词： chemical uniquely electronic